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The map label for this gene is lacA [C]

Identifier: 57652292

GI number: 57652292

Start: 2631968

End: 2632567

Strand: Reverse

Name: lacA [C]

Synonym: SACOL2570

Alternate gene names: 57652292

Gene position: 2632567-2631968 (Counterclockwise)

Preceding gene: 57652296

Following gene: 57652291

Centisome position: 93.7

GC content: 36.5

Gene sequence:

>600_bases
ATGACTGAAAAAGAAAAAATGTTAGCAGAAAAATGGTACGATGCAAACTTTGATCAATACTTAATCAATGAACGTGCACG
AGCGAAAGATATTTGCTTTGAATTAAATCATACAAGACCGAGTGCTACAAATAAAAGAAAGGAATTAATTGATCAATTAT
TTCAAACAACAACAGACAATGTAAGTATTTCGATTCCTTTTGATACAGATTATGGTTGGAACGTTAAACTAGGAAAAAAT
GTCTATGTAAACACCAATTGTTACTTTATGGATGGTGGACAGATTACAATTGGCGATAATGTTTTTATAGGACCTAATTG
TGGATTCTACACAGCGACACATCCACTTAATTTCCATCATAGAAATGAAGGTTTTGAAAAAGCCGGACCCATTCATATTG
GCAGTAATACTTGGTTTGGCGGTCATGTAGCCGTGTTACCAGGGGTGACGATTGGAGAAGGCAGTGTGATTGGTGCTGGT
AGTGTTGTCACTAAAGATATTCCGCCACACAGCTTAGCGGTTGGAAACCCTTGTAAAGTCGTTCGTAAAATTGATAATGA
CCTACCATCCGAAACATTAAATGACGAAACGATAAAGTAG

Upstream 100 bases:

>100_bases
TCATGATTTCATAATGTTATAGCGTATTAGTTGCATAGGCGAAGGTTTATTTGATTAACTTTTAAACAAGTAAATAAGTT
ATTAGGAGTTGAAAGGTGTA

Downstream 100 bases:

>100_bases
CAGTTGCGACCCATATAAATTTTAAAAAGTAACCAATCAATTTCAAAATACTTGAGAATAAGTGATAAAACATAAAAATG
AAAGCGATTGCAAAACTAAC

Product: galactoside O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKN
VYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK

Sequences:

>Translated_199_residues
MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKN
VYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK
>Mature_198_residues
TEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNV
YVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGS
VVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=186, Percent_Identity=42.4731182795699, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786664, Length=183, Percent_Identity=39.344262295082, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788367, Length=146, Percent_Identity=32.8767123287671, Blast_Score=72, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322243, Length=184, Percent_Identity=47.2826086956522, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22119; Mature: 21988

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATNKRKELIDQLFQTTTDN
CCHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
VSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHH
EEEEEECCCCCCCEEEECCEEEECCEEEEEECCEEEECCEEEECCCCCCEEECCCCCCCC
RNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKV
CCCCCCCCCCEEECCCCCCCCEEEEECCEEECCCCEEECCCEEECCCCCCCEECCCHHHH
VRKIDNDLPSETLNDETIK
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATNKRKELIDQLFQTTTDN
CHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
VSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHH
EEEEEECCCCCCCEEEECCEEEECCEEEEEECCEEEECCEEEECCCCCCEEECCCCCCCC
RNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKV
CCCCCCCCCCEEECCCCCCCCEEEEECCEEECCCCEEECCCEEECCCCCCCEECCCHHHH
VRKIDNDLPSETLNDETIK
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]