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The map label for this gene is hisF [H]

Identifier: 57651065

GI number: 57651065

Start: 2770889

End: 2771647

Strand: Reverse

Name: hisF [H]

Synonym: SACOL2697

Alternate gene names: 57651065

Gene position: 2771647-2770889 (Counterclockwise)

Preceding gene: 57651066

Following gene: 57651064

Centisome position: 98.66

GC content: 37.29

Gene sequence:

>759_bases
ATGATTAAAAAACGTATCATTCCATGTTTAGATGTCAAAGATGGTCGTGTCGTTAAAGGGATTCAATTTAAAGGATTAAG
GGATATTGGGAATCCTGTTGATTTAGCAATGTATTACAATGAAGCGGGTGCTGATGAATTAGTATTTTTAGACATCTCTA
AGACGGAAGAGGGTCATAGCTTAATGCTAGAAGTGATTGAACAGACAGCGTCACGCTTGTTTATCCCTCTTACTGTAGGG
GGTGGGATTCAAAGTCTCGATGATATTACCCAATTGCTAAATCATGGTGCAGATAAAGTATCATTAAATTCAAGTGCTTT
AAAAAATCCACAGCTCATTAAACAAGCGAGTGATAAATTCGGTAGACAATGCATCTGCATAGCAATTGATAGCTATTATG
ATCCTGAAAGAAAAGCACATTATTGTTGTACGACTGGTGGTAAAAAAATGACAAATATTAAAGTATATGACTGGGTACAG
CAAGTAGAACAGTTAGGTGCAGGTGAGCTCCTCGTTACAAGTATGGGACATGATGGTATGAAACAAGGCTTTGATATTGA
ACACCTAGCAAATATTAAGTCTCTTGTAAATATTCCAATCATTGCTTCTGGTGGTGGTGGCAATGCACAACACTTTGTAG
AATTATTTGATCAGACGGATGTTTCTGCAGGTTTAGCTGCAAGTATATTACATGATCGAGAAACGACGGTTCAATCTATT
AAAGAAGTGATACGGCAAGGGGGTATAGCAGTAAGATGA

Upstream 100 bases:

>100_bases
TTAGACATCAGCAAGATATTCAACGATTAGCGTCGCTAAATGTTCACGCTGCTATTATAGGAAAGGCTGCACATCAAGCA
TCTTTTTGGGAGGGGCTAAA

Downstream 100 bases:

>100_bases
CCAACTATAAAATTGATTTTAGCAAAGGTTTAGTGCCAGCAATTTTACAAGATAATCAAACAAAACAAGTATTGATGTTG
GGTTATATGAACCAAGAAGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR

Sequences:

>Translated_252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR
>Mature_252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=43.359375, Blast_Score=230, Evalue=6e-62,
Organism=Escherichia coli, GI87082028, Length=184, Percent_Identity=27.1739130434783, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=33.2268370607029, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27499; Mature: 27499

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHS
CCCCCCCCCCCCCCCCEEECEEECCHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCHHH
LMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKF
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGM
CCEEEEEEECCCCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHCCCCCEEEEECCCCHH
KQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
HCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHH
KEVIRQGGIAVR
HHHHHHCCCCCC
>Mature Secondary Structure
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHS
CCCCCCCCCCCCCCCCEEECEEECCHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCHHH
LMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKF
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGM
CCEEEEEEECCCCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHCCCCCEEEEECCCCHH
KQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
HCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHH
KEVIRQGGIAVR
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA