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The map label for this gene is menF [H]

Identifier: 57650236

GI number: 57650236

Start: 1056672

End: 1057883

Strand: Direct

Name: menF [H]

Synonym: SACOL1051

Alternate gene names: 57650236

Gene position: 1056672-1057883 (Clockwise)

Preceding gene: 57650235

Following gene: 57650237

Centisome position: 37.61

GC content: 30.86

Gene sequence:

>1212_bases
ATGCGTTTGAATGATAATCGAACGTCATATTTTGGCTACAAAGCAATTCAATTATTCAAAAATAATTCTAAAAATAAACA
ATCTATTTTTAAAGACTGGGAAAAATTAAAACATAACATCACATTTATACATCCGCAATCTGAGAAACATCATCTTCGAG
TTGTTGGAGGGTTCCAATTTTCAAGTCATAAATCAGATGATGAATGGCGAGAGTTTGGACTAAATCATTTTGTATTACCT
GAAGTTTTAATTTCAACTGATAATAATGGGACATTTTTAACTTATACAGTTAAAAGGGAAAGTTTTACTGTTGAGGCATT
GAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAG
ATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGCA
CGTAGACGGTTAATAAAGTTCGATAAAGATATCAGTATTCCATATATTCTAAAGCAAGCATATTCTAAAGAAAAAAACAG
TTATATATTCTTGTTAGAATCACAAGATTCTATATTCTTTTCACAAACACCTGAACAATTAATAAAGGTCAATAATAAAA
TACTATCGACTAAAGCTGTAGCAGGTACAATTAAACGTTCACAAGATGAGGACGAAGATACAAAAAATGTTGAAGCATTT
TTAAAAGATAATAAAAACTTAATCGAACATCGATTTGTTGTTGACAGTATTTTACATGATATTAAACCTTATATCACTGA
ATTACATTATGATAAGACGCCTAAAATTCTAAAAAATGATCATTTATATCACTTGTACACTGAAATAAAGGCGCCACTGA
AGGATGATTCGTATATTAGTTTAATTGATAATTTACATCCAACACCTGCTTTAGGTGGCTATCCAAAAGAATTTGCAATG
GATTTTATTGAGCAGAAAGAATTTGGTACGCGAGGATTATATGGTGCACCGGTTGGCTATATAGATATATACGATGATTG
TGAATTTATTGTTGCAATTCGTTCGATGCTTATTAAGAAAGCACAAGCAACTTTATTTGCTGGGTGTGGCATTGTTAAAG
ATTCTGATCCAGATAGTGAATTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAATGCATTAGGAGTCGATATGAAT
GGGAAATCATAA

Upstream 100 bases:

>100_bases
CTCAAAGGAATGGGTTTCAGTTGAAGTGAAATTATCACAGTCACTAGACCCGAGCACATTATTTCATCTCACTGACAATG
AGGCAGGAGATCGCTTTTAT

Downstream 100 bases:

>100_bases
AGCAGCTTTAACGAAGCAAGTTTTTACATTTGCATCTGAGTTATATGCGTACGGCGTAAGGGAAGTAGTTATCAGTCCGG
GATCACGCTCAACGCCACTT

Product: isochorismate synthase family protein

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLP
EVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLA
RRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAF
LKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAM
DFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
GKS

Sequences:

>Translated_403_residues
MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLP
EVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLA
RRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAF
LKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAM
DFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
GKS
>Mature_403_residues
MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLP
EVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLA
RRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAF
LKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAM
DFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
GKS

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=254, Percent_Identity=31.1023622047244, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082077, Length=265, Percent_Identity=29.0566037735849, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787518, Length=258, Percent_Identity=28.6821705426357, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788114, Length=199, Percent_Identity=28.1407035175879, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=209, Percent_Identity=31.1004784688995, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 46619; Mature: 46619

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQF
CCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEE
SSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDI
CCCCCCHHHHHHCCCCEECCEEEEEECCCCEEEEEEEECCCEEHHHHHHHHHHHCCCCCC
DVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQA
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
YSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAF
HCCCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYIS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEHHCCCCCCCCHHH
LIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKK
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
AQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS
HHHHHHHCCCCCCCCCCCHHHHHCCCEEEECHHHHCCCCCCCC
>Mature Secondary Structure
MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQF
CCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEE
SSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDI
CCCCCCHHHHHHCCCCEECCEEEEEECCCCEEEEEEEECCCEEHHHHHHHHHHHCCCCCC
DVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQA
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
YSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAF
HCCCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYIS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEHHCCCCCCCCHHH
LIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKK
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
AQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS
HHHHHHHCCCCCCCCCCCHHHHHCCCEEEECHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]