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The map label for this gene is menD [H]

Identifier: 57650237

GI number: 57650237

Start: 1057870

End: 1059543

Strand: Direct

Name: menD [H]

Synonym: SACOL1052

Alternate gene names: 57650237

Gene position: 1057870-1059543 (Clockwise)

Preceding gene: 57650236

Following gene: 57650238

Centisome position: 37.65

GC content: 33.99

Gene sequence:

>1674_bases
ATGGGAAATCATAAAGCAGCTTTAACGAAGCAAGTTTTTACATTTGCATCTGAGTTATATGCGTACGGCGTAAGGGAAGT
AGTTATCAGTCCGGGATCACGCTCAACGCCACTTGCACTTGCATTTGAAGCACATCCAAATATTAAAACATGGATACACC
CCGATGAGCGAAGTGCAGCGTTTTTTGCAGTTGGGTTAATTAAAGGCAGTGAAAGACCTGTCGCTATATTATGTACGTCA
GGTACAGCAGCAGCGAATTATACGCCTGCAATTGCTGAAAGCCAAATTAGTAGAATTCCTTTAATCGTTTTAACAAGTGA
CCGTCCGCATGAATTAAGAAGTGTAGGCGCACCACAAGCGATTAATCAAGTAAATATGTTTAATAATTATGTAAGTTATG
AGTTCGATATGCCTATTGCGGATGATAGTAAAGAGACCATTGATGCAATTTACTATCAAATGCAAATTGCTAGTCAATAT
TTATATGGACCACATAAAGGGCCAATTCATTTTAACTTGCCATTTAGAGATCCGTTAACACCTGATTTGAATGCAACAGA
ATTGTTAACTTCTGAGATGAAGATTTTACCGCACTATCAAAAAAGTATAGATGCATCGGCATTAAGACACATTTTAAATA
AGAAAAAAGGTTTAATTATTGTAGGGGATATGCAGCACCAAGAAGTTGATCAAATACTAACGTATTCAACGATATATGAT
TTGCCTATTTTAGCTGATCCTTTAAGTCATTTAAGAAAATTTGATCATCCGAATGTTATCTGTACATATGATTTGCTGTT
TAGAAGCGGCTTAGACTTAAATGTGGATTTCGTAATTCGTGTTGGGAAACCAGTGATTTCTAAAAAGTTAAATCAATGGT
TAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCA
TATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATG
GCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTG
AATTGATTAAGAAAACATCTGAAAAAGATGCATTATTTATTAGTAATAGTATGCCTATCAGAGATGTAGATAACTTGTTA
TTGAATAAAAATATAGATGTCTATGCGAATCGTGGTGCGAATGGTATTGATGGTATCGTTTCAACTGCACTGGGTATGGC
TGTGCATAAACGAATAACATTATTGATAGGTGATTTATCATTTTATCATGATATGAATGGACTATTAATGTCAAAATTAA
ATAATATTCAGATGAATATTGTATTATTGAACAACGATGGTGGCGGTATTTTTTCATATTTACCACAAAAAGAAAGTGCA
ACTGACTATTTTGAACGGTTGTTTGGCACACCGACGGGATTGGATTTCGAGTATACAGCTAAGTTATATCAATTCGATTT
TAAACGTTTTAACAGTGTTTCAGAATTTAAAAATGCCACATTGTTATCTGAAACTTCGACGATTTATGAATTGATAACGA
ATCGCGAAGATAACTTTAAACAGCATCAAATTTTATACCAGAAATTGAGTGAAATGATTCATGACACATTATAA

Upstream 100 bases:

>100_bases
GCTGGGTGTGGCATTGTTAAAGATTCTGATCCAGATAGTGAATTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAA
TGCATTAGGAGTCGATATGA

Downstream 100 bases:

>100_bases
ATTTTATGAAGCAAACGTTGAGACCAATCAAGTTTTAGTATTTCTGCATGGATTTCTTAGCGACAGCCGTACTTATCATA
ATCACATCGAAAAATTTACT

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTS
GTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYD
LPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDIS
YEISANDFFRSLMEDTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLL
LNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESA
TDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL

Sequences:

>Translated_557_residues
MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTS
GTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYD
LPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDIS
YEISANDFFRSLMEDTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLL
LNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESA
TDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL
>Mature_556_residues
GNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSG
TAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQYL
YGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYDL
PILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISY
EISANDFFRSLMEDTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLLL
NKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESAT
DYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=572, Percent_Identity=29.5454545454545, Blast_Score=215, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 63079; Mature: 62948

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAA
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCE
FFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQA
EEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHCCCCHH
INQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQYLYGPHKGPIHFNLPFRDPLT
HHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
PDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYD
CCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHC
LPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAF
CCHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEE
QILVQNNDKIDVFPIAPDISYEISANDFFRSLMEDTTVNRVSWLEKWQCLEKKGRKEIKC
EEEEECCCEEEEEECCCCCEEEECHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHH
YLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLLLNKNIDVYANRGANGIDGIV
HHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHH
STALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESA
HHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCH
TDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEHHHHHHHHHHHHCCCCCHH
QHQILYQKLSEMIHDTL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAA
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCE
FFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQA
EEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHCCCCHH
INQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQYLYGPHKGPIHFNLPFRDPLT
HHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
PDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYD
CCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHC
LPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAF
CCHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEE
QILVQNNDKIDVFPIAPDISYEISANDFFRSLMEDTTVNRVSWLEKWQCLEKKGRKEIKC
EEEEECCCEEEEEECCCCCEEEECHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHH
YLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLLLNKNIDVYANRGANGIDGIV
HHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHH
STALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESA
HHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCH
TDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEHHHHHHHHHHHHCCCCCHH
QHQILYQKLSEMIHDTL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA