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The map label for this gene is echA4

Identifier: 41410232

GI number: 41410232

Start: 4613382

End: 4614320

Strand: Direct

Name: echA4

Synonym: MAP4134

Alternate gene names: 41410232

Gene position: 4613382-4614320 (Clockwise)

Preceding gene: 41410231

Following gene: 41410233

Centisome position: 95.52

GC content: 68.9

Gene sequence:

>939_bases
ATGACCCACGCGATCAGGCCGGTCGACTTCGACAACCTGAAAACGATGACCTATGAGGTCACCGACCGGGTTGCCCGGAT
CACCTTCAACCGCCCGGAGAAGGGCAACGCGATCGTCGCGGACACCCCGCTGGAGCTCTCCGCGCTGGTGGAGCGGGCCG
ACCTGGATCCCAACGTGCACGTCATCCTGGTCTCCGGCCGCGGCGCGGGTTTCTGCGCGGGCTTCGACCTGAGCGCCTAC
GCCGATCGCACCGGCTCGGCCGGCGGGACCGGTGCCTACGCGGGCACCGTGCTGGACGGAAAAACCCAGGCGGTCAACCA
TTTAACCGATCAGCCGTGGGACCCGATGATCGACTATCAGATGATGAGCCGTTTCGTGCGGGGCTTCTCCTCCCTGATGC
ACGCCGACAAACCGACGGTGGTCAAGATCCACGGCTACTGCGTGGCCGGCGGCACCGACATCGCGTTGCACGCCGACCAG
GTGATCGCCGCCGCCGACGCCAAGATCGGCTACCCGCCGACGCGGGTGTGGGGCGTGCCGGCCGCCGGCCTGTGGGCGCA
CCGGCTGGGCGATCAGCGCGCCAAGCGGCTGCTGTTCACCGGCGATTGCATCACCGGCGCGCAAGCCGCCGAATGGGGTC
TGGCCGTCGAGGCGCCCGATCCGGCCGATCTCGACGAGCGCACCGAGCGGCTAGTGCAGCGGATCGCGGCGGTGCCGGTC
AACCAGCTGATCATGGTCAAGCTCGCGCTGAATTCCGCTCTGCTGCAACAGGGGGTGGCCACCAGCCGGATGGTCAGCAC
GGTGTTCGACGGCGTCGCCCGGCACACCCCGGAGGGCCACGCCTTCGTCGCCGACGCCGTGAAGCACGGATTCCGTGAGG
CGGTGCGGCATCGCGACGAACCGTTCGGCGACCACGGCCGGCAGGCCTCGCAGGTGTAG

Upstream 100 bases:

>100_bases
CTGGCGCCCATCCTCGAGCGGGCCCTGGTCAAGGGCTGAACCACAACATGACTTGCGCCGGCGACGATGCAGAGCACGGC
GATGGGGAGGAGCGGCGCCG

Downstream 100 bases:

>100_bases
CGATGCCGGATCTGACAGCGCGGTCGGTGGTGCTCAGCGTGCTGCTCGGTGCGCACCCCGCGCACGCCCGGGCCAGCGAA
TTGGTCAGGCTCACTTCCGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTHAIRPVDFDNLKTMTYEVTDRVARITFNRPEKGNAIVADTPLELSALVERADLDPNVHVILVSGRGAGFCAGFDLSAY
ADRTGSAGGTGAYAGTVLDGKTQAVNHLTDQPWDPMIDYQMMSRFVRGFSSLMHADKPTVVKIHGYCVAGGTDIALHADQ
VIAAADAKIGYPPTRVWGVPAAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPDPADLDERTERLVQRIAAVPV
NQLIMVKLALNSALLQQGVATSRMVSTVFDGVARHTPEGHAFVADAVKHGFREAVRHRDEPFGDHGRQASQV

Sequences:

>Translated_312_residues
MTHAIRPVDFDNLKTMTYEVTDRVARITFNRPEKGNAIVADTPLELSALVERADLDPNVHVILVSGRGAGFCAGFDLSAY
ADRTGSAGGTGAYAGTVLDGKTQAVNHLTDQPWDPMIDYQMMSRFVRGFSSLMHADKPTVVKIHGYCVAGGTDIALHADQ
VIAAADAKIGYPPTRVWGVPAAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPDPADLDERTERLVQRIAAVPV
NQLIMVKLALNSALLQQGVATSRMVSTVFDGVARHTPEGHAFVADAVKHGFREAVRHRDEPFGDHGRQASQV
>Mature_311_residues
THAIRPVDFDNLKTMTYEVTDRVARITFNRPEKGNAIVADTPLELSALVERADLDPNVHVILVSGRGAGFCAGFDLSAYA
DRTGSAGGTGAYAGTVLDGKTQAVNHLTDQPWDPMIDYQMMSRFVRGFSSLMHADKPTVVKIHGYCVAGGTDIALHADQV
IAAADAKIGYPPTRVWGVPAAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPDPADLDERTERLVQRIAAVPVN
QLIMVKLALNSALLQQGVATSRMVSTVFDGVARHTPEGHAFVADAVKHGFREAVRHRDEPFGDHGRQASQV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=26.953125, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=27.6, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787660, Length=234, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 33614; Mature: 33483

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHAIRPVDFDNLKTMTYEVTDRVARITFNRPEKGNAIVADTPLELSALVERADLDPNVH
CCCCCCCCCCCCCCEEEHHHHHHEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCEE
VILVSGRGAGFCAGFDLSAYADRTGSAGGTGAYAGTVLDGKTQAVNHLTDQPWDPMIDYQ
EEEEECCCCCEEECCCCHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCHH
MMSRFVRGFSSLMHADKPTVVKIHGYCVAGGTDIALHADQVIAAADAKIGYPPTRVWGVP
HHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEHHHHHEECCCCCCCCCCCCCCCC
AAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPDPADLDERTERLVQRIAAVPV
CHHHHHHHHHHHHHHEEEEECCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCC
NQLIMVKLALNSALLQQGVATSRMVSTVFDGVARHTPEGHAFVADAVKHGFREAVRHRDE
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
PFGDHGRQASQV
CCCCCCCCCCCC
>Mature Secondary Structure 
THAIRPVDFDNLKTMTYEVTDRVARITFNRPEKGNAIVADTPLELSALVERADLDPNVH
CCCCCCCCCCCCCEEEHHHHHHEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCEE
VILVSGRGAGFCAGFDLSAYADRTGSAGGTGAYAGTVLDGKTQAVNHLTDQPWDPMIDYQ
EEEEECCCCCEEECCCCHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCHH
MMSRFVRGFSSLMHADKPTVVKIHGYCVAGGTDIALHADQVIAAADAKIGYPPTRVWGVP
HHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEHHHHHEECCCCCCCCCCCCCCCC
AAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPDPADLDERTERLVQRIAAVPV
CHHHHHHHHHHHHHHEEEEECCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCC
NQLIMVKLALNSALLQQGVATSRMVSTVFDGVARHTPEGHAFVADAVKHGFREAVRHRDE
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
PFGDHGRQASQV
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA