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The map label for this gene is nfdA [H]

Identifier: 41410147

GI number: 41410147

Start: 4508816

End: 4510540

Strand: Direct

Name: nfdA [H]

Synonym: MAP4049

Alternate gene names: 41410147

Gene position: 4508816-4510540 (Clockwise)

Preceding gene: 41410145

Following gene: 41410148

Centisome position: 93.35

GC content: 70.32

Gene sequence:

>1725_bases
ATGGCGTGGCTCCTCCTCATCGCTTGGCTCTGCATCGTCGCCGGCGCGACTCATGGGGCCAGACTAGAACGTGTTGCAAT
TCCGGTCAGCCGCACCCTCGGACCGATAGCATCCGTGCACATGGGGCAGGCAGATCTAGTCCTCACCGGAACCGTACTGA
CCGTCGACGAGGCGCGGCCCACCGCCGAGGCGCTGGCCGTCGCCGACGGCCGCATCGTCGCCGTGGGAACCCGCGCCGAA
ATCGCGGCGCACGTCGGCCCGCACACCCAAACCGTCGACATCGGCGACGGCTGCGTGATGCCGGGATTCGTTGAGGCGCA
CGGGCATCCGCTGATGGAGGCGGTCGCGCTGTCGGACCGCATCGTGGACATCCGCCCGGTCACCCTGGCCAACGCGGACG
ACGTCGTCGCCGCCGTCAAGAGTGAGGTCGGCAAGCGGGGCGAAGCCGGTGCCTACCTCAACGGCTGGGATCCGCTGCTG
CAGCAGGGACTTCCGCAACCGACGCTGGCTTGGCTCGACGACATCGCGCCCGACGGCCCGCTGGTGATCATCCACAACTC
CGGGCACAAGGCGTTCTTCAACTCCCGAGCCGCCCAGCGGCACGGCCTGAACCGCGACACCCCCGACCCCAAGGGCGCCC
GCTACGGCCGCGACGCGGACGGCGAACTCGACGGCACCGCCGAGGAGACCGGCGCGGTGTTTCCGCTACTCGACGGCGCC
ATCGACACCGCTGGCTACCCGCGGATGCTGCACGCCGAGTGCGCCCGGCTGAACCGGGCCGGGCTGACCACCTGTTCGGA
GATGGCGTTCGACCCGCGGTTTCGGCCGCTGGTCGAACAACTGCGCGGGCAGCTGACGGTGCGGCTGCGCAGCTACGAGA
TCTCCAACCCGCAGCTGCGCACCGACGCCACCCTGGGCGAGGGCGACGACATGCTGCGCCAGGTCGGCATCAAGATCTGG
GTGGACGGCTCGCCCTGGATCGGCAACATCGCGCTGTCGTTCCCCTACCTGGACACCGAGGCCACCCGCACCATCGGCGT
CATCCCCGGCTCCTGCGGCTGCGCCAACTACACGACGGAGCAGTTGCACGAGATCGTCAGCGCCTACTTCCCGCTGGGCT
GGCAGCTGGCCTGCCACGTGCAGGGCGACGCCGGCGTCGACACGATCCTGGACGTCTACGAGGAGGCCCTCAAACGCCAT
CCGCGCGACGACCACCGGCTGCGGCTCGAGCACGTCGGCGCCATCCGGCCCGATCAACTCCAAAGGGCCGCCGACCTCGG
CGTCACCTGCAGCATCTTCGTCGACCAGATCCACTACTGGGGCGACGTCCTCGTCGACGGCCTGTTCGGCGAGGAACGCG
GATCCCGTTGGATGCCAGCGGGTTCCGCGGTGGCCACCGGCATGCGCATCTCGCTGCACAACGACCCGCCGGTCACCCCG
GAGGAGCCGCTGCGCAACATCAGCGTCGCCGCCACCCGGAAGGCGCCCAGTGGGCGGGTGCTGGCGCCCGAGGAGCGGCT
GACGGTCGAGCAGGCGATCCGGGCGCAGACCCTCGACGCGGCCTGGCAGCTGTTCGCCGACGACGTGATCGGCTCGCTCG
AGGTCGGCAAGTACGCCGACCTGGTGGTGCTGTCCGCCGACCCGCGCGCGGTGCCGCCCGAGCGGATCGCCGACCTGCAG
GTGCGGGCGACGTATCTGGCCGGCCGCCGGGTGTACCCACAGTGA

Upstream 100 bases:

>100_bases
GGTGTCGCCGAGATGCAGCACCGGCTTGTCTTTGTTGCGCTTGAGCGCACCGACGGTCAGGTGTCCGGAATGAATGGGAT
GGCGCAGCAGCTCGTCACCC

Downstream 100 bases:

>100_bases
TTCCGCCGCTGCAGGACCTGCTGGACCGGCTGCACGTGGTCGCGCTGCCGATGCGGGTGCGATTCCGCGGCATCACCACC
CGCGAGGTCGCGCTGATCGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MAWLLLIAWLCIVAGATHGARLERVAIPVSRTLGPIASVHMGQADLVLTGTVLTVDEARPTAEALAVADGRIVAVGTRAE
IAAHVGPHTQTVDIGDGCVMPGFVEAHGHPLMEAVALSDRIVDIRPVTLANADDVVAAVKSEVGKRGEAGAYLNGWDPLL
QQGLPQPTLAWLDDIAPDGPLVIIHNSGHKAFFNSRAAQRHGLNRDTPDPKGARYGRDADGELDGTAEETGAVFPLLDGA
IDTAGYPRMLHAECARLNRAGLTTCSEMAFDPRFRPLVEQLRGQLTVRLRSYEISNPQLRTDATLGEGDDMLRQVGIKIW
VDGSPWIGNIALSFPYLDTEATRTIGVIPGSCGCANYTTEQLHEIVSAYFPLGWQLACHVQGDAGVDTILDVYEEALKRH
PRDDHRLRLEHVGAIRPDQLQRAADLGVTCSIFVDQIHYWGDVLVDGLFGEERGSRWMPAGSAVATGMRISLHNDPPVTP
EEPLRNISVAATRKAPSGRVLAPEERLTVEQAIRAQTLDAAWQLFADDVIGSLEVGKYADLVVLSADPRAVPPERIADLQ
VRATYLAGRRVYPQ

Sequences:

>Translated_574_residues
MAWLLLIAWLCIVAGATHGARLERVAIPVSRTLGPIASVHMGQADLVLTGTVLTVDEARPTAEALAVADGRIVAVGTRAE
IAAHVGPHTQTVDIGDGCVMPGFVEAHGHPLMEAVALSDRIVDIRPVTLANADDVVAAVKSEVGKRGEAGAYLNGWDPLL
QQGLPQPTLAWLDDIAPDGPLVIIHNSGHKAFFNSRAAQRHGLNRDTPDPKGARYGRDADGELDGTAEETGAVFPLLDGA
IDTAGYPRMLHAECARLNRAGLTTCSEMAFDPRFRPLVEQLRGQLTVRLRSYEISNPQLRTDATLGEGDDMLRQVGIKIW
VDGSPWIGNIALSFPYLDTEATRTIGVIPGSCGCANYTTEQLHEIVSAYFPLGWQLACHVQGDAGVDTILDVYEEALKRH
PRDDHRLRLEHVGAIRPDQLQRAADLGVTCSIFVDQIHYWGDVLVDGLFGEERGSRWMPAGSAVATGMRISLHNDPPVTP
EEPLRNISVAATRKAPSGRVLAPEERLTVEQAIRAQTLDAAWQLFADDVIGSLEVGKYADLVVLSADPRAVPPERIADLQ
VRATYLAGRRVYPQ
>Mature_573_residues
AWLLLIAWLCIVAGATHGARLERVAIPVSRTLGPIASVHMGQADLVLTGTVLTVDEARPTAEALAVADGRIVAVGTRAEI
AAHVGPHTQTVDIGDGCVMPGFVEAHGHPLMEAVALSDRIVDIRPVTLANADDVVAAVKSEVGKRGEAGAYLNGWDPLLQ
QGLPQPTLAWLDDIAPDGPLVIIHNSGHKAFFNSRAAQRHGLNRDTPDPKGARYGRDADGELDGTAEETGAVFPLLDGAI
DTAGYPRMLHAECARLNRAGLTTCSEMAFDPRFRPLVEQLRGQLTVRLRSYEISNPQLRTDATLGEGDDMLRQVGIKIWV
DGSPWIGNIALSFPYLDTEATRTIGVIPGSCGCANYTTEQLHEIVSAYFPLGWQLACHVQGDAGVDTILDVYEEALKRHP
RDDHRLRLEHVGAIRPDQLQRAADLGVTCSIFVDQIHYWGDVLVDGLFGEERGSRWMPAGSAVATGMRISLHNDPPVTPE
EPLRNISVAATRKAPSGRVLAPEERLTVEQAIRAQTLDAAWQLFADDVIGSLEVGKYADLVVLSADPRAVPPERIADLQV
RATYLAGRRVYPQ

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 62009; Mature: 61878

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWLLLIAWLCIVAGATHGARLERVAIPVSRTLGPIASVHMGQADLVLTGTVLTVDEARP
CHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHCCHHHHCCCCCCEEEEEEEEEECCCCC
TAEALAVADGRIVAVGTRAEIAAHVGPHTQTVDIGDGCVMPGFVEAHGHPLMEAVALSDR
CHHHHEECCCEEEEEECHHHHHHHCCCCCEEEECCCCCCCCCCHHHCCCHHHHHHHHHCC
IVDIRPVTLANADDVVAAVKSEVGKRGEAGAYLNGWDPLLQQGLPQPTLAWLDDIAPDGP
EEEEEEEEECCCHHHHHHHHHHHCCCCCCCCEECCCHHHHHCCCCCCHHHHHHHCCCCCC
LVIIHNSGHKAFFNSRAAQRHGLNRDTPDPKGARYGRDADGELDGTAEETGAVFPLLDGA
EEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCC
IDTAGYPRMLHAECARLNRAGLTTCSEMAFDPRFRPLVEQLRGQLTVRLRSYEISNPQLR
CCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEE
TDATLGEGDDMLRQVGIKIWVDGSPWIGNIALSFPYLDTEATRTIGVIPGSCGCANYTTE
CCCCCCCCHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCHH
QLHEIVSAYFPLGWQLACHVQGDAGVDTILDVYEEALKRHPRDDHRLRLEHVGAIRPDQL
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCHHHH
QRAADLGVTCSIFVDQIHYWGDVLVDGLFGEERGSRWMPAGSAVATGMRISLHNDPPVTP
HHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCH
EEPLRNISVAATRKAPSGRVLAPEERLTVEQAIRAQTLDAAWQLFADDVIGSLEVGKYAD
HHHHHCCEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LVVLSADPRAVPPERIADLQVRATYLAGRRVYPQ
EEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
AWLLLIAWLCIVAGATHGARLERVAIPVSRTLGPIASVHMGQADLVLTGTVLTVDEARP
HHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHCCHHHHCCCCCCEEEEEEEEEECCCCC
TAEALAVADGRIVAVGTRAEIAAHVGPHTQTVDIGDGCVMPGFVEAHGHPLMEAVALSDR
CHHHHEECCCEEEEEECHHHHHHHCCCCCEEEECCCCCCCCCCHHHCCCHHHHHHHHHCC
IVDIRPVTLANADDVVAAVKSEVGKRGEAGAYLNGWDPLLQQGLPQPTLAWLDDIAPDGP
EEEEEEEEECCCHHHHHHHHHHHCCCCCCCCEECCCHHHHHCCCCCCHHHHHHHCCCCCC
LVIIHNSGHKAFFNSRAAQRHGLNRDTPDPKGARYGRDADGELDGTAEETGAVFPLLDGA
EEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCC
IDTAGYPRMLHAECARLNRAGLTTCSEMAFDPRFRPLVEQLRGQLTVRLRSYEISNPQLR
CCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEE
TDATLGEGDDMLRQVGIKIWVDGSPWIGNIALSFPYLDTEATRTIGVIPGSCGCANYTTE
CCCCCCCCHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCHH
QLHEIVSAYFPLGWQLACHVQGDAGVDTILDVYEEALKRHPRDDHRLRLEHVGAIRPDQL
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCHHHH
QRAADLGVTCSIFVDQIHYWGDVLVDGLFGEERGSRWMPAGSAVATGMRISLHNDPPVTP
HHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCH
EEPLRNISVAATRKAPSGRVLAPEERLTVEQAIRAQTLDAAWQLFADDVIGSLEVGKYAD
HHHHHCCEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LVVLSADPRAVPPERIADLQVRATYLAGRRVYPQ
EEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA