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The map label for this gene is hemB

Identifier: 41410103

GI number: 41410103

Start: 4466069

End: 4467049

Strand: Direct

Name: hemB

Synonym: MAP4005

Alternate gene names: 41410103

Gene position: 4466069-4467049 (Clockwise)

Preceding gene: 41410102

Following gene: 41410104

Centisome position: 92.47

GC content: 70.03

Gene sequence:

>981_bases
ATGGCTTACCCGCGGCAGCGCCCGCGCCGGCTTCGCTCGACCCCGGCGCTGCGCCGCCTGGTGGCCCAAACCTCCTTGGA
GCCAAGGCATTTGGTGCTGCCGATGTTCGTCGCCGACGGTATCGACGAACCGCGTCCGATCGCCTCGATGCCGAAGGTGG
TGCAGCACACCCGCGACTCGCTGCGCGCCGCCGCCGCCAGCGCCGTCGCCGCCGGGGTGGGCGGTCTGATGCTGTTCGGT
GTGCCGCGCGACGAGGACAAGGATCCGATCGGTTCGGCCGGCACCGACCCCGACGGCATCCTCAACGTCGCGCTGCGCGA
TCTGGCCAAGGACCTCGGCGATGCCACCGTGCTGATGGCCGACACCTGCCTGGACGAGTTCACCGACCACGGGCACTGCG
GTGTGCTGGATGACCGGGGCCGGGTGGACAACGACGCCACGGTGGCGCGCTACGTGAATCTCGCTGTGGCGCAAGCGGAG
TCGGGCGCCCACGTGGTCGGGCCGAGCGGGATGATGGACGGTCAGGTGGGCGCGATTCGCGACGGACTGGACGCCGCCGG
CTTCACCGACGTGGTGATCCTGGCCTACGCCGCCAAGTTCGCCTCGGCGTTCTACGGCCCGTTCCGGGAGGCGGTCAGCT
CCAGCCTGTCCGGGGATCGGCGCACGTATCAGCAGGAGACCGGCAACTCCCGCGAGGCGCTGCGCGAGATCCGGCTGGAC
CTCGACGAGGGCGCCGACATCGTGATGGTCAAGCCGGCGATGAGCTATCTGGACGTGGTGGCCGCCGCGGCGGAGGTCTC
GCCGGTCCCGGTGGCCGCCTACCAGGTGTCGGGGGAGTACGCGATGATTTGTGCTGCGGCCGCGAACAATTGGATCGACG
AGCGCGCCGCGGCGCTGGAGTCTCTGACCAGCATCCGACGCGCCGGAGCCGATATCGTTCTGAGCTATTGGGCCGCGGAT
GCGGCAGGGTGGCTGTCGTGA

Upstream 100 bases:

>100_bases
GGGCCCGCTGGTCGACGCTCTGGCCGAACACGCCGCGCGGTTGCGCGCCGAGGGGGCGCTGCCACCGCCGCGCAAGAAGA
GCCGCAGGCGCTAGCGTCTC

Downstream 100 bases:

>100_bases
GACGGGGCTTGCGTTGACATCAGACACGACCGGGGACGACGTCCAGCCGAAGCGGTTGTTCTACGAGCCGGGGGCCAGCT
GGTGGTGGGTGGCCTGCGGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MAYPRQRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDEPRPIASMPKVVQHTRDSLRAAAASAVAAGVGGLMLFG
VPRDEDKDPIGSAGTDPDGILNVALRDLAKDLGDATVLMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVNLAVAQAE
SGAHVVGPSGMMDGQVGAIRDGLDAAGFTDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQETGNSREALREIRLD
LDEGADIVMVKPAMSYLDVVAAAAEVSPVPVAAYQVSGEYAMICAAAANNWIDERAAALESLTSIRRAGADIVLSYWAAD
AAGWLS

Sequences:

>Translated_326_residues
MAYPRQRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDEPRPIASMPKVVQHTRDSLRAAAASAVAAGVGGLMLFG
VPRDEDKDPIGSAGTDPDGILNVALRDLAKDLGDATVLMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVNLAVAQAE
SGAHVVGPSGMMDGQVGAIRDGLDAAGFTDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQETGNSREALREIRLD
LDEGADIVMVKPAMSYLDVVAAAAEVSPVPVAAYQVSGEYAMICAAAANNWIDERAAALESLTSIRRAGADIVLSYWAAD
AAGWLS
>Mature_325_residues
AYPRQRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDEPRPIASMPKVVQHTRDSLRAAAASAVAAGVGGLMLFGV
PRDEDKDPIGSAGTDPDGILNVALRDLAKDLGDATVLMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVNLAVAQAES
GAHVVGPSGMMDGQVGAIRDGLDAAGFTDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQETGNSREALREIRLDL
DEGADIVMVKPAMSYLDVVAAAAEVSPVPVAAYQVSGEYAMICAAAANNWIDERAAALESLTSIRRAGADIVLSYWAADA
AGWLS

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=309, Percent_Identity=41.1003236245955, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI87081728, Length=317, Percent_Identity=48.5804416403785, Blast_Score=276, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6321398, Length=317, Percent_Identity=37.8548895899054, Blast_Score=214, Evalue=1e-56,
Organism=Drosophila melanogaster, GI21358291, Length=315, Percent_Identity=39.0476190476191, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 34543; Mature: 34412

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYPRQRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDEPRPIASMPKVVQHTRDS
CCCCCHHHHHHHCCHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCHHHHHHHHHHHHHH
LRAAAASAVAAGVGGLMLFGVPRDEDKDPIGSAGTDPDGILNVALRDLAKDLGDATVLMA
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHH
DTCLDEFTDHGHCGVLDDRGRVDNDATVARYVNLAVAQAESGAHVVGPSGMMDGQVGAIR
HHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHH
DGLDAAGFTDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQETGNSREALREIRLD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
LDEGADIVMVKPAMSYLDVVAAAAEVSPVPVAAYQVSGEYAMICAAAANNWIDERAAALE
HCCCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEHHHHCCHHHHHHHHHH
SLTSIRRAGADIVLSYWAADAAGWLS
HHHHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AYPRQRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDEPRPIASMPKVVQHTRDS
CCCCHHHHHHHCCHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCHHHHHHHHHHHHHH
LRAAAASAVAAGVGGLMLFGVPRDEDKDPIGSAGTDPDGILNVALRDLAKDLGDATVLMA
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHH
DTCLDEFTDHGHCGVLDDRGRVDNDATVARYVNLAVAQAESGAHVVGPSGMMDGQVGAIR
HHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHH
DGLDAAGFTDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQETGNSREALREIRLD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
LDEGADIVMVKPAMSYLDVVAAAAEVSPVPVAAYQVSGEYAMICAAAANNWIDERAAALE
HCCCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEHHHHCCHHHHHHHHHH
SLTSIRRAGADIVLSYWAADAAGWLS
HHHHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]