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The map label for this gene is cysG

Identifier: 41410102

GI number: 41410102

Start: 4464404

End: 4466062

Strand: Direct

Name: cysG

Synonym: MAP4004

Alternate gene names: 41410102

Gene position: 4464404-4466062 (Clockwise)

Preceding gene: 41410101

Following gene: 41410103

Centisome position: 92.43

GC content: 73.24

Gene sequence:

>1659_bases
ATGACGACGCGAGGGCGTAAGCCGACACCCGGCCGCATCACGTACGTGGGCTCCGGCCCGGGTGACCCGGGACTGTTGAC
GGCCCGGGCCGCGACGGTGCTGGCGAACGCCGCGCTGGTGTTCACCGACCCCGACGTGCCCGAGCCGGTGCTGGCGCTGA
TCGGCAAGGACCTGCCACCGGTGTCCGGCCCGGCGCCGGCCGAGCCGGCGTCCAACGGCGAGGGCTCCGGCTCCGAGCAG
GGGCAGGCGCAGCCGGCGGTGATCTCCGGTGGCCCGGACATCCGCCCGGCGCTGGGTGATCCCGCCGAGGTGGCCAAGAC
GCTGACCGCCGAGGCGCGCACCGGTGTCGACGTGGTGCGGCTGGTGGCCGGTGACCCGCTGACCGTCGACGCCGTCATCA
CCGAGGTCAACGCCATCGCCCGCAGCCACCTGCACATCGAGATCGTGCCCGGGCTGGCGCCCACCAACGCCGTCCCGACC
TACGCGGGCCTGCCGCTGGGCTCCTCGCACACCATGGCCGACGTGCGCGGTGACGTGGACTGGGAGGCGCTGGCCGCCGC
GCCCGGCCCGCTGATCCTGCAGGCCACCGCGTCGCACCTGGCCGACGCGGCGCGCACCCTGATCGAACACGAACTGGCCG
AGAACACCCCGTGCGTGGTCACCGCGCACGGCACCACCTGCCAGCAGCGCTCCATCGAGACCACGCTGCAGGGGCTGACC
GACTCGGCGGTGCTGGGCGGCACCGACCCCGCCGGCCCGCTGACCGGTCCGCTGGTGGTGACCATCGGCAAGACGGTGAG
CGCCCGGACCAAGCTGAACTGGTGGGAGAGCCGCGCCCTGTACGGCTGGACCGTGCTGGTGCCCCGCACCAAGGACCAGG
CCGGCGAGATGAGCGAGCGGCTGACGTCGTACGGCGCGCTGCCGATCGAGGTGCCGACCATCGCCGTCGAGCCGCCGCGC
AGCCCCGCGCAGATGGAGCGTGCCGTCAAGGGATTGGTCGACGGCCGCTTCCAGTGGGTGGTGTTCACCTCCACCAACGC
GGTGCGCGCGGTGTGGGAGAAGTTCGGCGAGTTCGGTCTGGACGCGCGCGCGTTCTCCGGGGTGAAGATCGCCTGTGTGG
GCGAGTCGACGGCCGACCGGGTGCGGGCGTTCGGGATCAGCCCCGAGCTGGTGCCGGCCGGCGAGCAGTCCTCGCTGGGT
CTGCTCGACGACTTCCCGCCCTACGACAGCGTTTTCGACCCGGTGAACCGGGTGCTGTTGCCGCGGGCCGACATCGCCAC
CGAGACGCTGGCCGAGGGGCTGCGCGAACGGGGTTGGGAGATCGAGGATGTCACGGCCTACCGGACGGTGCGGGCCGCGC
CGCCGCCGGCGGAGACCCGGGAGATGATCAAGACCGGCGGCTTCGACGCGGTGTGCTTCACGTCCAGCTCCACGGTGCGC
AACCTGGTCGGCATCGCCGGCAAGCCGCACGCCCGGACGATCATCGCCTGCATCGGTCCCAAGACCGCCGAGACCGCCGC
CGAGTTCGGCCTGCGGGTGGATGTCCAGCCCGAGACCGCGGCGGTGGGCCCGCTGGTCGACGCTCTGGCCGAACACGCCG
CGCGGTTGCGCGCCGAGGGGGCGCTGCCACCGCCGCGCAAGAAGAGCCGCAGGCGCTAG

Upstream 100 bases:

>100_bases
TCGCCGCCGAGCTGTTCGAGCTGGGCGCGCGCGAGTTGATGAGCGGAGCGCGGCACGACCCGGCGCGAGGAAATTAAGTG
CGAGTTACCTGGGAGCGACA

Downstream 100 bases:

>100_bases
CGTCTCATGGCTTACCCGCGGCAGCGCCCGCGCCGGCTTCGCTCGACCCCGGCGCTGCGCCGCCTGGTGGCCCAAACCTC
CTTGGAGCCAAGGCATTTGG

Product: hypothetical protein

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MTTRGRKPTPGRITYVGSGPGDPGLLTARAATVLANAALVFTDPDVPEPVLALIGKDLPPVSGPAPAEPASNGEGSGSEQ
GQAQPAVISGGPDIRPALGDPAEVAKTLTAEARTGVDVVRLVAGDPLTVDAVITEVNAIARSHLHIEIVPGLAPTNAVPT
YAGLPLGSSHTMADVRGDVDWEALAAAPGPLILQATASHLADAARTLIEHELAENTPCVVTAHGTTCQQRSIETTLQGLT
DSAVLGGTDPAGPLTGPLVVTIGKTVSARTKLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGALPIEVPTIAVEPPR
SPAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGISPELVPAGEQSSLG
LLDDFPPYDSVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPAETREMIKTGGFDAVCFTSSSTVR
NLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPETAAVGPLVDALAEHAARLRAEGALPPPRKKSRRR

Sequences:

>Translated_552_residues
MTTRGRKPTPGRITYVGSGPGDPGLLTARAATVLANAALVFTDPDVPEPVLALIGKDLPPVSGPAPAEPASNGEGSGSEQ
GQAQPAVISGGPDIRPALGDPAEVAKTLTAEARTGVDVVRLVAGDPLTVDAVITEVNAIARSHLHIEIVPGLAPTNAVPT
YAGLPLGSSHTMADVRGDVDWEALAAAPGPLILQATASHLADAARTLIEHELAENTPCVVTAHGTTCQQRSIETTLQGLT
DSAVLGGTDPAGPLTGPLVVTIGKTVSARTKLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGALPIEVPTIAVEPPR
SPAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGISPELVPAGEQSSLG
LLDDFPPYDSVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPAETREMIKTGGFDAVCFTSSSTVR
NLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPETAAVGPLVDALAEHAARLRAEGALPPPRKKSRRR
>Mature_551_residues
TTRGRKPTPGRITYVGSGPGDPGLLTARAATVLANAALVFTDPDVPEPVLALIGKDLPPVSGPAPAEPASNGEGSGSEQG
QAQPAVISGGPDIRPALGDPAEVAKTLTAEARTGVDVVRLVAGDPLTVDAVITEVNAIARSHLHIEIVPGLAPTNAVPTY
AGLPLGSSHTMADVRGDVDWEALAAAPGPLILQATASHLADAARTLIEHELAENTPCVVTAHGTTCQQRSIETTLQGLTD
SAVLGGTDPAGPLTGPLVVTIGKTVSARTKLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGALPIEVPTIAVEPPRS
PAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGISPELVPAGEQSSLGL
LDDFPPYDSVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPAETREMIKTGGFDAVCFTSSSTVRN
LVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPETAAVGPLVDALAEHAARLRAEGALPPPRKKSRRR

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=277, Percent_Identity=27.0758122743682, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 57768; Mature: 57637

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRGRKPTPGRITYVGSGPGDPGLLTARAATVLANAALVFTDPDVPEPVLALIGKDLPP
CCCCCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCCC
VSGPAPAEPASNGEGSGSEQGQAQPAVISGGPDIRPALGDPAEVAKTLTAEARTGVDVVR
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LVAGDPLTVDAVITEVNAIARSHLHIEIVPGLAPTNAVPTYAGLPLGSSHTMADVRGDVD
ECCCCCEEHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
WEALAAAPGPLILQATASHLADAARTLIEHELAENTPCVVTAHGTTCQQRSIETTLQGLT
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCC
DSAVLGGTDPAGPLTGPLVVTIGKTVSARTKLNWWESRALYGWTVLVPRTKDQAGEMSER
CCCEECCCCCCCCCCCCEEEEECCCEECCEECCHHHCCCEEEEEEEEECCCCHHHHHHHH
LTSYGALPIEVPTIAVEPPRSPAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFGEFGL
HHHCCCCEEECCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCC
DARAFSGVKIACVGESTADRVRAFGISPELVPAGEQSSLGLLDDFPPYDSVFDPVNRVLL
CCHHCCCEEEEEECCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPAETREMIKTGGFDAVCFTSSSTVR
CCCHHHHHHHHHHHHHCCCCCEEEHHEEEEECCCCCHHHHHHHHHCCCCEEEEECCHHHH
NLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPETAAVGPLVDALAEHAARLRAEG
HHHHCCCCCCCCEEEEEECCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCC
ALPPPRKKSRRR
CCCCCCHHCCCC
>Mature Secondary Structure 
TTRGRKPTPGRITYVGSGPGDPGLLTARAATVLANAALVFTDPDVPEPVLALIGKDLPP
CCCCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCCC
VSGPAPAEPASNGEGSGSEQGQAQPAVISGGPDIRPALGDPAEVAKTLTAEARTGVDVVR
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LVAGDPLTVDAVITEVNAIARSHLHIEIVPGLAPTNAVPTYAGLPLGSSHTMADVRGDVD
ECCCCCEEHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
WEALAAAPGPLILQATASHLADAARTLIEHELAENTPCVVTAHGTTCQQRSIETTLQGLT
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCC
DSAVLGGTDPAGPLTGPLVVTIGKTVSARTKLNWWESRALYGWTVLVPRTKDQAGEMSER
CCCEECCCCCCCCCCCCEEEEECCCEECCEECCHHHCCCEEEEEEEEECCCCHHHHHHHH
LTSYGALPIEVPTIAVEPPRSPAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFGEFGL
HHHCCCCEEECCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCC
DARAFSGVKIACVGESTADRVRAFGISPELVPAGEQSSLGLLDDFPPYDSVFDPVNRVLL
CCHHCCCEEEEEECCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPAETREMIKTGGFDAVCFTSSSTVR
CCCHHHHHHHHHHHHHCCCCCEEEHHEEEEECCCCCHHHHHHHHHCCCCEEEEECCHHHH
NLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPETAAVGPLVDALAEHAARLRAEG
HHHHCCCCCCCCEEEEEECCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCC
ALPPPRKKSRRR
CCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]