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The map label for this gene is metZ

Identifier: 41409971

GI number: 41409971

Start: 4334202

End: 4335428

Strand: Direct

Name: metZ

Synonym: MAP3873

Alternate gene names: 41409971

Gene position: 4334202-4335428 (Clockwise)

Preceding gene: 41409970

Following gene: 41409978

Centisome position: 89.74

GC content: 68.62

Gene sequence:

>1227_bases
ATGAGTCAGGCCGGCGACGATTCGGTCCGCACGCCCCCCGCGCTGCCCGACGGCGTCAGCCAGGCGACCATCGGCGTGCG
CGGCGGACTACTGCGTTCGGAATTCGACGAGACCGCCGAGGCGCTGTATCTGACCTCCGGCTACGTCTACGAGTCGGCCG
CCGTTGCCGAGCAGTCGTTCACCGGCGAACTGGACCATTTCGTCTACTCCCGCTACGGCAACCCCACCGTGACCATGTTC
GAGGAGCGGCTGCGCCTGCTCGAAGGCGCGCCGGCGGCGTTCGCCACCGCGAGCGGCATGGCGGCGGTGTTCACCTCGCT
GGGCGCGCTGCTGGCCGCGGGCGACCGGCTGGTCGCCGCGCGCAGCCTGTTCGGCTCCTGCTTCGTGGTGTGCAACGAGA
TCCTGCCGCGCTGGGGCGTGCAGACCGTGTTCGTCGACGGCGACGACCTCGCGCAGTGGGAGGAGGCGTTGTCGGTGCCG
ACGGCGGCGGTGTTCTTCGAGACGCCGTCGAACCCGATGCAGTCGCTGGTGGACATCGCCGCGGTCACCGAGCTCGCCCA
CGCCGCGGGCGCAAAGGTGGTGCTGGACAACGTTTTCGCCACCCCGTTGCTGCAGCAGGGCATTCCGCTCGGGGTGGACG
TGGTGGTGTACTCGGGTACCAAACACATCGACGGCCAGGGCCGGGTGCTGGGCGGGGCCATCCTCGGGGACAGGGACTAC
ATCGACGGGCCGGTGCAGAAGCTGATGCGGCACACCGGTCCGGCGATGAGCGCGTTCAACGCCTGGGTGCTGCTGAAAGG
CCTTGAGACCATGGCCATCCGGGTGGAACACAGCAATTCCTCGGCGCACCGGATCGCCGAATTCCTGGAGACCCATCCCG
CGGTGCGCTGGGTCCGCTACCCGTATCTGCCGTCCCACCCGCAGTACGACCTGGCCAAGCGCCAGATGTCCGGCGGCGGA
ACGGTGATCACCTTCGCGCTGGACTGCCCCGACGACAAGGCCAAGCAGCGGGCCTTCGAGGTGCTGGACAAGCTGACGCT
GATCGACATCTCCAACAACCTCGGTGACGCCAAATCCCTTGTCACACATCCCGCTACCACCACCCACCGGGCGATGGGCC
CGGAGGGCCGTGCGGCGATCGGGCTGGGCGACGGCGTGGTCCGCATCTCGGTCGGGCTGGAAGGCACCGACGACTTGATC
GCCGACATCGACCGGGCGCTGGGTTAG

Upstream 100 bases:

>100_bases
ACGTGCTGGACGGCTTCGAGGGTCATCTCGACGCCAACGGCCACCGCGGTGAAACAGGTTGGCGGGCAATCGGTTTGCCC
TGGAAGCAGGGATAGCCCGG

Downstream 100 bases:

>100_bases
CCGGGTCAGCCGGCCTGCTTTTCGGCCGAGTCGACGTGCTCGGCGGCCGCCAGCGTCCCGTCCTGGGCCTGGCGCTCCAG
CCACTCCACCACCGGCCGCG

Product: O-succinylhomoserine sulfhydrylase

Products: cystathionine; succinate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSQAGDDSVRTPPALPDGVSQATIGVRGGLLRSEFDETAEALYLTSGYVYESAAVAEQSFTGELDHFVYSRYGNPTVTMF
EERLRLLEGAPAAFATASGMAAVFTSLGALLAAGDRLVAARSLFGSCFVVCNEILPRWGVQTVFVDGDDLAQWEEALSVP
TAAVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGIPLGVDVVVYSGTKHIDGQGRVLGGAILGDRDY
IDGPVQKLMRHTGPAMSAFNAWVLLKGLETMAIRVEHSNSSAHRIAEFLETHPAVRWVRYPYLPSHPQYDLAKRQMSGGG
TVITFALDCPDDKAKQRAFEVLDKLTLIDISNNLGDAKSLVTHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEGTDDLI
ADIDRALG

Sequences:

>Translated_408_residues
MSQAGDDSVRTPPALPDGVSQATIGVRGGLLRSEFDETAEALYLTSGYVYESAAVAEQSFTGELDHFVYSRYGNPTVTMF
EERLRLLEGAPAAFATASGMAAVFTSLGALLAAGDRLVAARSLFGSCFVVCNEILPRWGVQTVFVDGDDLAQWEEALSVP
TAAVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGIPLGVDVVVYSGTKHIDGQGRVLGGAILGDRDY
IDGPVQKLMRHTGPAMSAFNAWVLLKGLETMAIRVEHSNSSAHRIAEFLETHPAVRWVRYPYLPSHPQYDLAKRQMSGGG
TVITFALDCPDDKAKQRAFEVLDKLTLIDISNNLGDAKSLVTHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEGTDDLI
ADIDRALG
>Mature_407_residues
SQAGDDSVRTPPALPDGVSQATIGVRGGLLRSEFDETAEALYLTSGYVYESAAVAEQSFTGELDHFVYSRYGNPTVTMFE
ERLRLLEGAPAAFATASGMAAVFTSLGALLAAGDRLVAARSLFGSCFVVCNEILPRWGVQTVFVDGDDLAQWEEALSVPT
AAVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGIPLGVDVVVYSGTKHIDGQGRVLGGAILGDRDYI
DGPVQKLMRHTGPAMSAFNAWVLLKGLETMAIRVEHSNSSAHRIAEFLETHPAVRWVRYPYLPSHPQYDLAKRQMSGGGT
VITFALDCPDDKAKQRAFEVLDKLTLIDISNNLGDAKSLVTHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEGTDDLIA
DIDRALG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=337, Percent_Identity=32.9376854599407, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI299473759, Length=337, Percent_Identity=29.3768545994065, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI25453487, Length=336, Percent_Identity=28.2738095238095, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1790375, Length=388, Percent_Identity=35.0515463917526, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1789383, Length=350, Percent_Identity=26.5714285714286, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560056, Length=341, Percent_Identity=34.8973607038123, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=341, Percent_Identity=34.8973607038123, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=380, Percent_Identity=30.2631578947368, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323333, Length=407, Percent_Identity=33.1695331695332, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6319307, Length=348, Percent_Identity=34.7701149425287, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=378, Percent_Identity=25.6613756613757, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=263, Percent_Identity=26.615969581749, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=344, Percent_Identity=25.2906976744186, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922554, Length=353, Percent_Identity=32.5779036827195, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48

Molecular weight: Translated: 43472; Mature: 43340

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAGDDSVRTPPALPDGVSQATIGVRGGLLRSEFDETAEALYLTSGYVYESAAVAEQSF
CCCCCCCCCCCCCCCCCCCCHHEHHHCCCHHHHHHHHHHHEEEEECCCEEHHHHHHHHHC
TGELDHFVYSRYGNPTVTMFEERLRLLEGAPAAFATASGMAAVFTSLGALLAAGDRLVAA
CCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RSLFGSCFVVCNEILPRWGVQTVFVDGDDLAQWEEALSVPTAAVFFETPSNPMQSLVDIA
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
AVTELAHAAGAKVVLDNVFATPLLQQGIPLGVDVVVYSGTKHIDGQGRVLGGAILGDRDY
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCEEEEEEEECCCCC
IDGPVQKLMRHTGPAMSAFNAWVLLKGLETMAIRVEHSNSSAHRIAEFLETHPAVRWVRY
CCHHHHHHHHHCCHHHHHHHHHHHEECCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEC
PYLPSHPQYDLAKRQMSGGGTVITFALDCPDDKAKQRAFEVLDKLTLIDISNNLGDAKSL
CCCCCCCCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHEEEEEECCCCCCHHHH
VTHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEGTDDLIADIDRALG
HCCCCCCCHHCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQAGDDSVRTPPALPDGVSQATIGVRGGLLRSEFDETAEALYLTSGYVYESAAVAEQSF
CCCCCCCCCCCCCCCCCCCHHEHHHCCCHHHHHHHHHHHEEEEECCCEEHHHHHHHHHC
TGELDHFVYSRYGNPTVTMFEERLRLLEGAPAAFATASGMAAVFTSLGALLAAGDRLVAA
CCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RSLFGSCFVVCNEILPRWGVQTVFVDGDDLAQWEEALSVPTAAVFFETPSNPMQSLVDIA
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
AVTELAHAAGAKVVLDNVFATPLLQQGIPLGVDVVVYSGTKHIDGQGRVLGGAILGDRDY
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCEEEEEEEECCCCC
IDGPVQKLMRHTGPAMSAFNAWVLLKGLETMAIRVEHSNSSAHRIAEFLETHPAVRWVRY
CCHHHHHHHHHCCHHHHHHHHHHHEECCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEC
PYLPSHPQYDLAKRQMSGGGTVITFALDCPDDKAKQRAFEVLDKLTLIDISNNLGDAKSL
CCCCCCCCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHEEEEEECCCCCCHHHH
VTHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEGTDDLIADIDRALG
HCCCCCCCHHCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; L-cysteine

Specific reaction: O-succinyl-L-homoserine + L-cysteine = cystathionine + succinate

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]