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The map label for this gene is echA1_2

Identifier: 41409756

GI number: 41409756

Start: 4063585

End: 4064379

Strand: Direct

Name: echA1_2

Synonym: MAP3658

Alternate gene names: 41409756

Gene position: 4063585-4064379 (Clockwise)

Preceding gene: 41409755

Following gene: 41409757

Centisome position: 84.14

GC content: 71.19

Gene sequence:

>795_bases
GTGCGGCACGTGAGCACTGGGAACGAAGAAGCCGGCGAACCCGAGGTCCTGGTCGAACAACGGGATCGGATCCTCATCAT
CACGATCAACCGGCCGAAGGCCAAGAACGCGGTCAACGCCGCGGTGGCCAACGGACTGGCCGCCGCCGTCGACCGGCTGG
ACAACGAGCCCGGTCTGTCGGTCGGCGTCCTGACCGGGGCCGGCGGCTCCTTCTGCGCGGGTATGGATCTGAAGGCCTTC
GCCCGCGGCGAGCTGCCCATCGTCGAGGGCCGCGGCATGGGTTTCACCGAACGCCCGCCGGCCAAGCCGCTGATCGCGGC
GGTCGAGGGTTACGCGCTGGCCGGCGGCACCGAGCTGGCGCTGGCCACCGACCTGATCGTCGCCTCCAAGGACGCGGCCT
TCGGCATTCCGGAAGTCAAGCGCGGCCTGGTCGCCGGGGGCGGCGGGCTGCTGCGGCTGCCGCAGCGCATCCCGTCCGCG
ATCGCCATGGAGCTGGCGCTGACCGGTGACAACCTCAGCGCCGAGCGGGCGCACGCGCTCGGCATGGTCAACGTGCTGGC
CGAGCCGGGTGCCGCGCTGGACGAGGCGATCGCGCTGGCCGAGAAGATCGCGGCGAACGGGCCGCTGGCGGTGGCCGCCA
CCAAGCGGATCATCGTCGAGTCCCGCGGCTGGAGCCCGGAGACCATGTTCGCCGAGCAGAACAAGCTGCTGGCCCCCGTG
TTCTCCTCCAACGACGCCAAGGAAGGCGCCATCGCGTTCGCCGAGAAGCGGCCCCCCAAGTGGACGGGCACCTGA

Upstream 100 bases:

>100_bases
GCGGTAACCGGAATCTCGGGGTACGTCAACGCCGTTCCGGCCGACCGTTACCATCGGTCCGACGGTCGCAGTTGGTGCGC
CACCGATACCCGAAGGAGCT

Downstream 100 bases:

>100_bases
TCGGTCCCGGGCGGTGATCTAAGCTACAGTCGGACATGACACTTTTGGCCGGCTTTTTGTAACGTGCTGACACCGCAGGA
AGACCGAGGATGAGCATTTC

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRHVSTGNEEAGEPEVLVEQRDRILIITINRPKAKNAVNAAVANGLAAAVDRLDNEPGLSVGVLTGAGGSFCAGMDLKAF
ARGELPIVEGRGMGFTERPPAKPLIAAVEGYALAGGTELALATDLIVASKDAAFGIPEVKRGLVAGGGGLLRLPQRIPSA
IAMELALTGDNLSAERAHALGMVNVLAEPGAALDEAIALAEKIAANGPLAVAATKRIIVESRGWSPETMFAEQNKLLAPV
FSSNDAKEGAIAFAEKRPPKWTGT

Sequences:

>Translated_264_residues
MRHVSTGNEEAGEPEVLVEQRDRILIITINRPKAKNAVNAAVANGLAAAVDRLDNEPGLSVGVLTGAGGSFCAGMDLKAF
ARGELPIVEGRGMGFTERPPAKPLIAAVEGYALAGGTELALATDLIVASKDAAFGIPEVKRGLVAGGGGLLRLPQRIPSA
IAMELALTGDNLSAERAHALGMVNVLAEPGAALDEAIALAEKIAANGPLAVAATKRIIVESRGWSPETMFAEQNKLLAPV
FSSNDAKEGAIAFAEKRPPKWTGT
>Mature_264_residues
MRHVSTGNEEAGEPEVLVEQRDRILIITINRPKAKNAVNAAVANGLAAAVDRLDNEPGLSVGVLTGAGGSFCAGMDLKAF
ARGELPIVEGRGMGFTERPPAKPLIAAVEGYALAGGTELALATDLIVASKDAAFGIPEVKRGLVAGGGGLLRLPQRIPSA
IAMELALTGDNLSAERAHALGMVNVLAEPGAALDEAIALAEKIAANGPLAVAATKRIIVESRGWSPETMFAEQNKLLAPV
FSSNDAKEGAIAFAEKRPPKWTGT

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=35.8267716535433, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI70995211, Length=224, Percent_Identity=32.5892857142857, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=33.852140077821, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=26.4, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI150378541, Length=248, Percent_Identity=32.6612903225806, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37594471, Length=208, Percent_Identity=27.4038461538462, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=28.9772727272727, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594469, Length=208, Percent_Identity=27.4038461538462, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68989263, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI221142681, Length=249, Percent_Identity=42.1686746987952, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=34.6303501945525, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=31.8725099601594, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=27.9527559055118, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=28.0632411067194, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=34.0314136125654, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790281, Length=204, Percent_Identity=29.9019607843137, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=37.6518218623482, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=35.0194552529183, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560910, Length=246, Percent_Identity=34.5528455284553, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=212, Percent_Identity=36.7924528301887, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=26.7716535433071, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=27.7056277056277, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=29.6703296703297, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=222, Percent_Identity=30.6306306306306, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=222, Percent_Identity=30.6306306306306, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=222, Percent_Identity=30.6306306306306, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=252, Percent_Identity=26.984126984127, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=185, Percent_Identity=25.9459459459459, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=35.632183908046, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=35.632183908046, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=34.8484848484849, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21357171, Length=273, Percent_Identity=31.1355311355311, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650670, Length=196, Percent_Identity=36.734693877551, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19920382, Length=218, Percent_Identity=30.2752293577982, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=27.734375, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921000, Length=177, Percent_Identity=37.8531073446328, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583077, Length=177, Percent_Identity=37.8531073446328, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583079, Length=177, Percent_Identity=37.8531073446328, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24654903, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27215; Mature: 27215

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHVSTGNEEAGEPEVLVEQRDRILIITINRPKAKNAVNAAVANGLAAAVDRLDNEPGLS
CCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
VGVLTGAGGSFCAGMDLKAFARGELPIVEGRGMGFTERPPAKPLIAAVEGYALAGGTELA
EEEEECCCCCEEECCCHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHCCEEECCCCEEE
LATDLIVASKDAAFGIPEVKRGLVAGGGGLLRLPQRIPSAIAMELALTGDNLSAERAHAL
EEEEEEEECCCCCCCCHHHHHCEEECCCCEEECHHHCCHHHEEEEEEECCCCCHHHHHHH
GMVNVLAEPGAALDEAIALAEKIAANGPLAVAATKRIIVESRGWSPETMFAEQNKLLAPV
HHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCHHEECCCCCEEEEE
FSSNDAKEGAIAFAEKRPPKWTGT
ECCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MRHVSTGNEEAGEPEVLVEQRDRILIITINRPKAKNAVNAAVANGLAAAVDRLDNEPGLS
CCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
VGVLTGAGGSFCAGMDLKAFARGELPIVEGRGMGFTERPPAKPLIAAVEGYALAGGTELA
EEEEECCCCCEEECCCHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHCCEEECCCCEEE
LATDLIVASKDAAFGIPEVKRGLVAGGGGLLRLPQRIPSAIAMELALTGDNLSAERAHAL
EEEEEEEECCCCCCCCHHHHHCEEECCCCEEECHHHCCHHHEEEEEEECCCCCHHHHHHH
GMVNVLAEPGAALDEAIALAEKIAANGPLAVAATKRIIVESRGWSPETMFAEQNKLLAPV
HHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCHHEECCCCCEEEEE
FSSNDAKEGAIAFAEKRPPKWTGT
ECCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA