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The map label for this gene is lpdA

Identifier: 41409522

GI number: 41409522

Start: 3803135

End: 3804613

Strand: Reverse

Name: lpdA

Synonym: MAP3424c

Alternate gene names: 41409522

Gene position: 3804613-3803135 (Counterclockwise)

Preceding gene: 41409524

Following gene: 41409521

Centisome position: 78.77

GC content: 72.01

Gene sequence:

>1479_bases
ATGGCCCGCCTCCTCCTCCCGCCGCGCTGCGGCGGGCATCGTCGCCGGGCTAGGTTGGGGGTCGTGGTGACCCGCATCGT
GATCCTCGGCGGAGGCCCGGCCGGTTACGAAGCCGCCCTGGTGGCCGCCTCCTCCCACCCCGACAGCACCCACGTCACGG
TGATCGACTCCGAGGGAATCGGCGGCGCGGCCGTGCTGGACGACTGCGTGCCGTCCAAGACATTCATCGCCTCGACCTGG
CTGCGCACCGAGCTGCGCCGCGCGCCGCGGCTGGGCTTCGAGATCGACATCGACGACGCCAAGATCTCGCTGCCCCAGAT
CCACGCCCGGGTCAAGCAACTGGCCACCGAGCAGTCCGCCGACATCACCGAACAGCTGCTCGGCGTGGGCGTGCACGTGG
TCGCCGGCCGCGGCGAGCTGATCGACCCCACCCCGGGGCTGGCCCGCCACCGCATCAAGGCCACCGCCCCCGACGGCAGC
AGCAGCGAGTACGAGGCCGACGTCGTGCTGATCGCCACCGGCGCCAGCCCGCGGATCCTGCCGTCGGCCCAGCCCGACGG
CGAGCGGATCCTGACCTGGCGCCAGCTCTACAACCTCGACGCGCTGCCCGACCACCTCATCGTGGTCGGCTCGGGGGTGA
CCGGCGCCGAGTTCGTGCACGCCTACACCGAATTGGGCGTGCCGGTGACCGTGGTGGCCAGCCGCGACCGGGTGCTGCCC
TACGAGGACGCCGACGCCGCCCTGGTGCTGGAAGAGGCGTTCTCCGAGCGCGGCGTGCAGCTGGTGAAGAACGCGCGCGC
GCAATCGGTCACCCGCACGCCCGACGGGGTGCTGGTCACCCTGACCGACGGGCGCACCGTGGCGGGCAGCCACGCCCTGA
TGACTATCGGCTCGGTGCCCAACACCGCCGGCCTGGGCCTGGAGCGGGTCGGCATCGAGCTGGGCCGCGGCGGCTACCTG
ACGGTGGACCGGGTGTCGCGGACCTCGGTGCCCGGCATCTACGCCGCCGGCGACTGCACCGGCCTGCTGCCGCTGGCCTC
GGTGGCCGCCATGCAGGGCCGCATCGCGATGTATCACGCGCTCGGCGAGGGCGTCAGCCCGATCCGGCTGCGCACCGTCG
CGGCGACGGTGTTCACCCGGCCCGAGATCGCCGCCGTCGGCGTGCCGCAGACCATGATCGACGACGGTTCGGTGTCGGCG
CGCACCATCATGCTGCCGCTGCGCACCAATGCGCGGGCCAAGATGTCGGGGCTGCGGCAGGGTTTCGTGAAGGTGTTCTG
CCGCAAGTCCACCGGGGTGGTGATCGGCGGCGTGGTGGTGGCGCCGATCGCCTCCGAGCTGATCCTGCCGATCGCGGTGG
CGGTGCAGAACCGCATCACCGTCAACGAATTGGCGCAGACGCTGGCGGTTTACCCCTCGCTGTCCGGTTCGATCACCGAG
GCGGCCCGGCGGCTGATGGCCCACGACGACCTGGACTAA

Upstream 100 bases:

>100_bases
AGAGCGGCACGGGGAAAGCTTAGACGTCCGGGCTGCCTCGGTCTTCCCCCGAGCCGGGCGCCGCCGCGCTGCGGCGGGCA
TCGTCGCCGGGCTGGGTGGG

Downstream 100 bases:

>100_bases
CGGTTAGCCTTGGTGCAGCACGCCCTACTGACGAGTAACCGGACAGGAGCCCTGCAGTGAGCGATCCGATCCACGCACTC
GGCGAGCCCGGCTCGCCGGC

Product: flavoprotein disulfide reductase

Products: NA

Alternate protein names: NAD(P)H quinone reductase; NAD(P)H: menadione oxidoreductase; NADH-menadione reductase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MARLLLPPRCGGHRRRARLGVVVTRIVILGGGPAGYEAALVAASSHPDSTHVTVIDSEGIGGAAVLDDCVPSKTFIASTW
LRTELRRAPRLGFEIDIDDAKISLPQIHARVKQLATEQSADITEQLLGVGVHVVAGRGELIDPTPGLARHRIKATAPDGS
SSEYEADVVLIATGASPRILPSAQPDGERILTWRQLYNLDALPDHLIVVGSGVTGAEFVHAYTELGVPVTVVASRDRVLP
YEDADAALVLEEAFSERGVQLVKNARAQSVTRTPDGVLVTLTDGRTVAGSHALMTIGSVPNTAGLGLERVGIELGRGGYL
TVDRVSRTSVPGIYAAGDCTGLLPLASVAAMQGRIAMYHALGEGVSPIRLRTVAATVFTRPEIAAVGVPQTMIDDGSVSA
RTIMLPLRTNARAKMSGLRQGFVKVFCRKSTGVVIGGVVVAPIASELILPIAVAVQNRITVNELAQTLAVYPSLSGSITE
AARRLMAHDDLD

Sequences:

>Translated_492_residues
MARLLLPPRCGGHRRRARLGVVVTRIVILGGGPAGYEAALVAASSHPDSTHVTVIDSEGIGGAAVLDDCVPSKTFIASTW
LRTELRRAPRLGFEIDIDDAKISLPQIHARVKQLATEQSADITEQLLGVGVHVVAGRGELIDPTPGLARHRIKATAPDGS
SSEYEADVVLIATGASPRILPSAQPDGERILTWRQLYNLDALPDHLIVVGSGVTGAEFVHAYTELGVPVTVVASRDRVLP
YEDADAALVLEEAFSERGVQLVKNARAQSVTRTPDGVLVTLTDGRTVAGSHALMTIGSVPNTAGLGLERVGIELGRGGYL
TVDRVSRTSVPGIYAAGDCTGLLPLASVAAMQGRIAMYHALGEGVSPIRLRTVAATVFTRPEIAAVGVPQTMIDDGSVSA
RTIMLPLRTNARAKMSGLRQGFVKVFCRKSTGVVIGGVVVAPIASELILPIAVAVQNRITVNELAQTLAVYPSLSGSITE
AARRLMAHDDLD
>Mature_491_residues
ARLLLPPRCGGHRRRARLGVVVTRIVILGGGPAGYEAALVAASSHPDSTHVTVIDSEGIGGAAVLDDCVPSKTFIASTWL
RTELRRAPRLGFEIDIDDAKISLPQIHARVKQLATEQSADITEQLLGVGVHVVAGRGELIDPTPGLARHRIKATAPDGSS
SEYEADVVLIATGASPRILPSAQPDGERILTWRQLYNLDALPDHLIVVGSGVTGAEFVHAYTELGVPVTVVASRDRVLPY
EDADAALVLEEAFSERGVQLVKNARAQSVTRTPDGVLVTLTDGRTVAGSHALMTIGSVPNTAGLGLERVGIELGRGGYLT
VDRVSRTSVPGIYAAGDCTGLLPLASVAAMQGRIAMYHALGEGVSPIRLRTVAATVFTRPEIAAVGVPQTMIDDGSVSAR
TIMLPLRTNARAKMSGLRQGFVKVFCRKSTGVVIGGVVVAPIASELILPIAVAVQNRITVNELAQTLAVYPSLSGSITEA
ARRLMAHDDLD

Specific function: May contribute to virulence by increasing resistance to reactive oxygen intermediates. It can reduce 2,6-dimethyl-1,4- benzoquinone (DMBQ), 5-hydroxy-1,4-naphthaquinone (5-HNQ) and menadione. NAD(P)H is the physiological reductant rather than NADH [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=25.4859611231102, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI50301238, Length=439, Percent_Identity=25.2847380410023, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=443, Percent_Identity=25.5079006772009, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI22035672, Length=439, Percent_Identity=24.8291571753986, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI148277071, Length=434, Percent_Identity=24.6543778801843, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI33519430, Length=434, Percent_Identity=24.6543778801843, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI33519428, Length=434, Percent_Identity=24.6543778801843, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI33519426, Length=434, Percent_Identity=24.6543778801843, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI148277065, Length=434, Percent_Identity=24.6543778801843, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI291045268, Length=438, Percent_Identity=25.1141552511416, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1786307, Length=466, Percent_Identity=26.6094420600858, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=25.0530785562633, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=24.295010845987, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=25.3456221198157, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789065, Length=235, Percent_Identity=25.531914893617, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=26.3498920086393, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71983419, Length=435, Percent_Identity=26.4367816091954, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71983429, Length=435, Percent_Identity=26.4367816091954, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=25.0526315789474, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=26.0416666666667, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325166, Length=436, Percent_Identity=22.7064220183486, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325240, Length=491, Percent_Identity=23.0142566191446, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=26.4392324093817, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24640553, Length=493, Percent_Identity=26.5720081135903, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640549, Length=493, Percent_Identity=25.7606490872211, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640551, Length=493, Percent_Identity=26.369168356998, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=25.2566735112936, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.5.2 [H]

Molecular weight: Translated: 51743; Mature: 51612

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLLLPPRCGGHRRRARLGVVVTRIVILGGGPAGYEAALVAASSHPDSTHVTVIDSEGI
CCCEECCCCCCCCHHHHHHHHEEEEEEEECCCCCCCEEEEEEECCCCCCEEEEEEECCCC
GGAAVLDDCVPSKTFIASTWLRTELRRAPRLGFEIDIDDAKISLPQIHARVKQLATEQSA
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCEECHHHHHHHHHHHHHHCCC
DITEQLLGVGVHVVAGRGELIDPTPGLARHRIKATAPDGSSSEYEADVVLIATGASPRIL
HHHHHHHHCCEEEEECCCCCCCCCCCHHHCEEEEECCCCCCCCEEEEEEEEECCCCCCCC
PSAQPDGERILTWRQLYNLDALPDHLIVVGSGVTGAEFVHAYTELGVPVTVVASRDRVLP
CCCCCCCHHEEEHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
YEDADAALVLEEAFSERGVQLVKNARAQSVTRTPDGVLVTLTDGRTVAGSHALMTIGSVP
CCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCEEEEECCCC
NTAGLGLERVGIELGRGGYLTVDRVSRTSVPGIYAAGDCTGLLPLASVAAMQGRIAMYHA
CCCCCCHHHHCEEECCCCEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCEEEEEH
LGEGVSPIRLRTVAATVFTRPEIAAVGVPQTMIDDGSVSARTIMLPLRTNARAKMSGLRQ
HCCCCCCEEEEEEEHHHCCCCCEEEECCCHHEECCCCCCEEEEEEEECCCCHHHHHHHHH
GFVKVFCRKSTGVVIGGVVVAPIASELILPIAVAVQNRITVNELAQTLAVYPSLSGSITE
HHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
AARRLMAHDDLD
HHHHHHHCCCCC
>Mature Secondary Structure 
ARLLLPPRCGGHRRRARLGVVVTRIVILGGGPAGYEAALVAASSHPDSTHVTVIDSEGI
CCEECCCCCCCCHHHHHHHHEEEEEEEECCCCCCCEEEEEEECCCCCCEEEEEEECCCC
GGAAVLDDCVPSKTFIASTWLRTELRRAPRLGFEIDIDDAKISLPQIHARVKQLATEQSA
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCEECHHHHHHHHHHHHHHCCC
DITEQLLGVGVHVVAGRGELIDPTPGLARHRIKATAPDGSSSEYEADVVLIATGASPRIL
HHHHHHHHCCEEEEECCCCCCCCCCCHHHCEEEEECCCCCCCCEEEEEEEEECCCCCCCC
PSAQPDGERILTWRQLYNLDALPDHLIVVGSGVTGAEFVHAYTELGVPVTVVASRDRVLP
CCCCCCCHHEEEHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
YEDADAALVLEEAFSERGVQLVKNARAQSVTRTPDGVLVTLTDGRTVAGSHALMTIGSVP
CCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCEEEEECCCC
NTAGLGLERVGIELGRGGYLTVDRVSRTSVPGIYAAGDCTGLLPLASVAAMQGRIAMYHA
CCCCCCHHHHCEEECCCCEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCEEEEEH
LGEGVSPIRLRTVAATVFTRPEIAAVGVPQTMIDDGSVSARTIMLPLRTNARAKMSGLRQ
HCCCCCCEEEEEEEHHHCCCCCEEEECCCHHEECCCCCCEEEEEEEECCCCHHHHHHHHH
GFVKVFCRKSTGVVIGGVVVAPIASELILPIAVAVQNRITVNELAQTLAVYPSLSGSITE
HHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
AARRLMAHDDLD
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]