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The map label for this gene is amiA

Identifier: 41409524

GI number: 41409524

Start: 3805194

End: 3806372

Strand: Reverse

Name: amiA

Synonym: MAP3426c

Alternate gene names: NA

Gene position: 3806372-3805194 (Counterclockwise)

Preceding gene: 41409525

Following gene: 41409522

Centisome position: 78.81

GC content: 72.6

Gene sequence:

>1179_bases
ATGAGCCTGGCCGACGCCGCCACCTCCTGGCTGGCCGCGCACAACCAAGACCTGATCGAGTGGCGTCGGCACATCCACCG
CTATCCCGAGTTGGGCCGCCAGGAATACGCCACCACCCAGTTCGTCGCCGAGCGGTTGGCCGAGGCCGGACTCAACCCCA
AGGTGCTGCCCGGCGGGACCGGGCTGACCTGCGACCTCGGCCCCGAGCACGAGCCCCGGATCGCGCTGCGGGCCGACATG
GACGCGCTGCCGATGGCCGAGCGCACCGGCGCGCCCTACGCCTCCACCATGCCCAACGTGGCGCACGCCTGCGGGCACGA
CGCGCACACCGCCATCCTGCTGGGCACCGCGCTGGTGCTGGCGTCGGTGCCGGAACTGCCGGTCGGGGTGCGGTTGATCT
TCCAGGCCGCCGAGGAACTGATGCCCGGCGGGGCGATCGACGCCATCGCCGCCGGCGCCATCACCGGGGTGTCGCGGATC
TTCGCCCTGCACTGCGACCCCCGGCTGGAGGTCGGCCAGATCGCGGTCCGGCACGGCCCGATCACCTCGGCGGCCGACCA
GATCGAGATCACCCTGTATTCGCCGGGCGGGCACACCTCGCGGCCGCACCTGACCGCCGACCTGGTGTACGGGCTGGGCA
CCCTGATCACCGGGCTGCCCGGGGTGCTGTCGCGGCGCATCGAACCGCGCAACGGCACCGTGCTGGTGTGGGGCGCGGTC
AACGCCGGGGTGGCGGCCAACGCCATCCCGCAGACCGGGGTGCTGGCCGGCACCGTGCGCACCGCCAGCCGGCAGACCTG
GGTGGGTCTCGAGGAGGTGATCCGGGAGACGGTGTCCGGGCTGCTCGCGCCGCTGGGCATCGAGCACACGCTGCAGTACC
GGCGCGGCGTGCCGCCGGTGGTCAACGAGGACGTCTCGACGCGCATCCTCACCCACGCGATCGAGGCGCTCGGCCCCGAC
GCCCTGGCCGACACCCGCCAGTCCGGTGGCGGCGAAGACTTCTCCTGGTACCTCGAGGAGATTCCCGGCGCGATGGCGCG
GCTGGGGGTGTGGCCCGGGGTGGGGCCACAGCTGGACCTGCACCAGCCGACGTTCAACCTCGACGAGCGGGCCCTGGCGA
TCGGGGTGCGGGTGATGGCCAACATCGTCGAACAGTCGGCGCTGTTCCAGGTGTTCTAG

Upstream 100 bases:

>100_bases
CAATCATGCTGGCCCGCACCGTCGTCGCGCTGGCCCAGACGCCGGCCGAGCGCGATCGGGTGCTGGCCGCGCACGAGCGC
AGACGCGGCGGGGCGCACCC

Downstream 100 bases:

>100_bases
GCCGCTAGGCCGCGACGGAAAGTCAGGGCCCGATGTTGCGGGCCGGGCGGGTGCGCAGGTCGTGCACGTAATCGGCTGGC
GCGCCGGCGATTTCGGCGGC

Product: AmiA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSLADAATSWLAAHNQDLIEWRRHIHRYPELGRQEYATTQFVAERLAEAGLNPKVLPGGTGLTCDLGPEHEPRIALRADM
DALPMAERTGAPYASTMPNVAHACGHDAHTAILLGTALVLASVPELPVGVRLIFQAAEELMPGGAIDAIAAGAITGVSRI
FALHCDPRLEVGQIAVRHGPITSAADQIEITLYSPGGHTSRPHLTADLVYGLGTLITGLPGVLSRRIEPRNGTVLVWGAV
NAGVAANAIPQTGVLAGTVRTASRQTWVGLEEVIRETVSGLLAPLGIEHTLQYRRGVPPVVNEDVSTRILTHAIEALGPD
ALADTRQSGGGEDFSWYLEEIPGAMARLGVWPGVGPQLDLHQPTFNLDERALAIGVRVMANIVEQSALFQVF

Sequences:

>Translated_392_residues
MSLADAATSWLAAHNQDLIEWRRHIHRYPELGRQEYATTQFVAERLAEAGLNPKVLPGGTGLTCDLGPEHEPRIALRADM
DALPMAERTGAPYASTMPNVAHACGHDAHTAILLGTALVLASVPELPVGVRLIFQAAEELMPGGAIDAIAAGAITGVSRI
FALHCDPRLEVGQIAVRHGPITSAADQIEITLYSPGGHTSRPHLTADLVYGLGTLITGLPGVLSRRIEPRNGTVLVWGAV
NAGVAANAIPQTGVLAGTVRTASRQTWVGLEEVIRETVSGLLAPLGIEHTLQYRRGVPPVVNEDVSTRILTHAIEALGPD
ALADTRQSGGGEDFSWYLEEIPGAMARLGVWPGVGPQLDLHQPTFNLDERALAIGVRVMANIVEQSALFQVF
>Mature_391_residues
SLADAATSWLAAHNQDLIEWRRHIHRYPELGRQEYATTQFVAERLAEAGLNPKVLPGGTGLTCDLGPEHEPRIALRADMD
ALPMAERTGAPYASTMPNVAHACGHDAHTAILLGTALVLASVPELPVGVRLIFQAAEELMPGGAIDAIAAGAITGVSRIF
ALHCDPRLEVGQIAVRHGPITSAADQIEITLYSPGGHTSRPHLTADLVYGLGTLITGLPGVLSRRIEPRNGTVLVWGAVN
AGVAANAIPQTGVLAGTVRTASRQTWVGLEEVIRETVSGLLAPLGIEHTLQYRRGVPPVVNEDVSTRILTHAIEALGPDA
LADTRQSGGGEDFSWYLEEIPGAMARLGVWPGVGPQLDLHQPTFNLDERALAIGVRVMANIVEQSALFQVF

Specific function: ATP-dependent carboxylate-amine ligase [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the peptidase M20 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011793
- InterPro:   IPR006336
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF04107 GCS2; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 41658; Mature: 41527

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLADAATSWLAAHNQDLIEWRRHIHRYPELGRQEYATTQFVAERLAEAGLNPKVLPGGT
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GLTCDLGPEHEPRIALRADMDALPMAERTGAPYASTMPNVAHACGHDAHTAILLGTALVL
CCEECCCCCCCCCEEEECCCCCCCCHHHCCCCCHHHCCHHHHHCCCCHHHHHHHHHHHHH
ASVPELPVGVRLIFQAAEELMPGGAIDAIAAGAITGVSRIFALHCDPRLEVGQIAVRHGP
HCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHEEEECCC
ITSAADQIEITLYSPGGHTSRPHLTADLVYGLGTLITGLPGVLSRRIEPRNGTVLVWGAV
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEECC
NAGVAANAIPQTGVLAGTVRTASRQTWVGLEEVIRETVSGLLAPLGIEHTLQYRRGVPPV
CCCCHHCCCCCCCCEEEHHHHCCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
VNEDVSTRILTHAIEALGPDALADTRQSGGGEDFSWYLEEIPGAMARLGVWPGVGPQLDL
CCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCC
HQPTFNLDERALAIGVRVMANIVEQSALFQVF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLADAATSWLAAHNQDLIEWRRHIHRYPELGRQEYATTQFVAERLAEAGLNPKVLPGGT
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GLTCDLGPEHEPRIALRADMDALPMAERTGAPYASTMPNVAHACGHDAHTAILLGTALVL
CCEECCCCCCCCCEEEECCCCCCCCHHHCCCCCHHHCCHHHHHCCCCHHHHHHHHHHHHH
ASVPELPVGVRLIFQAAEELMPGGAIDAIAAGAITGVSRIFALHCDPRLEVGQIAVRHGP
HCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHEEEECCC
ITSAADQIEITLYSPGGHTSRPHLTADLVYGLGTLITGLPGVLSRRIEPRNGTVLVWGAV
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEECC
NAGVAANAIPQTGVLAGTVRTASRQTWVGLEEVIRETVSGLLAPLGIEHTLQYRRGVPPV
CCCCHHCCCCCCCCEEEHHHHCCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
VNEDVSTRILTHAIEALGPDALADTRQSGGGEDFSWYLEEIPGAMARLGVWPGVGPQLDL
CCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCC
HQPTFNLDERALAIGVRVMANIVEQSALFQVF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA