Click here to switch to the map view.

The map label for this gene is nei

Identifier: 41409514

GI number: 41409514

Start: 3794265

End: 3795017

Strand: Direct

Name: nei

Synonym: MAP3416

Alternate gene names: 41409514

Gene position: 3794265-3795017 (Clockwise)

Preceding gene: 41409513

Following gene: 41409516

Centisome position: 78.56

GC content: 71.98

Gene sequence:

>753_bases
ATGCCGGAGGGTGACACCGTCTGGCACACCGCCGCCGTGCTGCGCGAGCATCTGCTCGGCGAGACGCTGACCCGCTGCGA
CATCCGGGTGCCGCGGTTCGCCACCGTCGACCTCACCGGGCAGGTGGTCGACGAGATCCTCAGCCGCGGAAAGCATCTGT
TCATCCGGGTGGGCGCCGCCAGCATCCACTCGCACCTGAAGATGGAGGGCAGCTGGCGGGTCGGCCCGCGGGTGCGGGTC
GACCACCGGGCGCGCATCGTCCTGGAAACCGGCGCCGCGACCGCGGTCGGCGTCGATCTGGGCGTGCTGCAGATCCTGGA
CCGCGATCGCGACGGCGAGGCCGTCGCGCATCTGGGACCCGATCTGCTCGGCGAGGATTGGGACCCCGCGCGGGCCGCCG
CCAACCTGGCGGCCCGCCCGCAGCGGCCGATCGCCGAGGCGCTGCTGGATCAGCGGGTGCTGGCCGGGATCGGCAACGTC
TACTGCAACGAGTTGTGCTTTGTCAGCGGGCATCTGCCCACCACGCCGGTGAGCGCCGTCGCCGACCCGCGGCGGCTCGT
CTCGCGTGCCCGGGACATGTTGTGGCTCAACCGCTTCCGTTGGAACCGGTGCACCACCGGCGACACCCGCAACGGCCGGC
AGCTGTGGGTCTACGGGCGGGCCGGCCAACCGTGCCGCCGCTGCGGCACCCCCATCGAGTTCGACGACTCCGGGGACCGG
GTGACCTACTGGTGCCCGTCCTGCCAGCGTTAA

Upstream 100 bases:

>100_bases
TGGCGCCGCGCGGCGACGGTGCCGCCGGCAACGACGCGCTGACGGCGCTCGCCGACGCCGGGTTCGCCCGCACCCCGCGC
GGAATGCGGCTGCGCTGACC

Downstream 100 bases:

>100_bases
TCGCGGGCGTGGGCCAGGAAGAACTCCGCGATGGCCTCCGACCCGTCCAGCGTGTGCGTGGTGGGGCCGATGACCGCGGC
GGGCAGGTACTGCTTGCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase Rv3297/MT3396; Putative AP lyase Rv3297/MT3396 [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPEGDTVWHTAAVLREHLLGETLTRCDIRVPRFATVDLTGQVVDEILSRGKHLFIRVGAASIHSHLKMEGSWRVGPRVRV
DHRARIVLETGAATAVGVDLGVLQILDRDRDGEAVAHLGPDLLGEDWDPARAAANLAARPQRPIAEALLDQRVLAGIGNV
YCNELCFVSGHLPTTPVSAVADPRRLVSRARDMLWLNRFRWNRCTTGDTRNGRQLWVYGRAGQPCRRCGTPIEFDDSGDR
VTYWCPSCQR

Sequences:

>Translated_250_residues
MPEGDTVWHTAAVLREHLLGETLTRCDIRVPRFATVDLTGQVVDEILSRGKHLFIRVGAASIHSHLKMEGSWRVGPRVRV
DHRARIVLETGAATAVGVDLGVLQILDRDRDGEAVAHLGPDLLGEDWDPARAAANLAARPQRPIAEALLDQRVLAGIGNV
YCNELCFVSGHLPTTPVSAVADPRRLVSRARDMLWLNRFRWNRCTTGDTRNGRQLWVYGRAGQPCRRCGTPIEFDDSGDR
VTYWCPSCQR
>Mature_249_residues
PEGDTVWHTAAVLREHLLGETLTRCDIRVPRFATVDLTGQVVDEILSRGKHLFIRVGAASIHSHLKMEGSWRVGPRVRVD
HRARIVLETGAATAVGVDLGVLQILDRDRDGEAVAHLGPDLLGEDWDPARAAANLAARPQRPIAEALLDQRVLAGIGNVY
CNELCFVSGHLPTTPVSAVADPRRLVSRARDMLWLNRFRWNRCTTGDTRNGRQLWVYGRAGQPCRRCGTPIEFDDSGDRV
TYWCPSCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1790066, Length=280, Percent_Identity=27.1428571428571, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786932, Length=263, Percent_Identity=28.1368821292776, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27801; Mature: 27670

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01242 ZF_FPG_1 ; PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGDTVWHTAAVLREHLLGETLTRCDIRVPRFATVDLTGQVVDEILSRGKHLFIRVGAA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCEEEEEECHH
SIHSHLKMEGSWRVGPRVRVDHRARIVLETGAATAVGVDLGVLQILDRDRDGEAVAHLGP
HHHHHHEECCCCCCCCEEEECCCEEEEEECCCCEEEHHHHHHHHHHCCCCCCCCHHHCCH
DLLGEDWDPARAAANLAARPQRPIAEALLDQRVLAGIGNVYCNELCFVSGHLPTTPVSAV
HHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHH
ADPRRLVSRARDMLWLNRFRWNRCTTGDTRNGRQLWVYGRAGQPCRRCGTPIEFDDSGDR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCEECCCCCE
VTYWCPSCQR
EEEECCCCCC
>Mature Secondary Structure 
PEGDTVWHTAAVLREHLLGETLTRCDIRVPRFATVDLTGQVVDEILSRGKHLFIRVGAA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCEEEEEECHH
SIHSHLKMEGSWRVGPRVRVDHRARIVLETGAATAVGVDLGVLQILDRDRDGEAVAHLGP
HHHHHHEECCCCCCCCEEEECCCEEEEEECCCCEEEHHHHHHHHHHCCCCCCCCHHHCCH
DLLGEDWDPARAAANLAARPQRPIAEALLDQRVLAGIGNVYCNELCFVSGHLPTTPVSAV
HHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHH
ADPRRLVSRARDMLWLNRFRWNRCTTGDTRNGRQLWVYGRAGQPCRRCGTPIEFDDSGDR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCEECCCCCE
VTYWCPSCQR
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]