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The map label for this gene is kstD [H]

Identifier: 41409496

GI number: 41409496

Start: 3773500

End: 3775038

Strand: Reverse

Name: kstD [H]

Synonym: MAP3398c

Alternate gene names: 41409496

Gene position: 3775038-3773500 (Counterclockwise)

Preceding gene: 41409504

Following gene: 41409495

Centisome position: 78.16

GC content: 72.97

Gene sequence:

>1539_bases
GTGGACCGCGGAGTGCGACGTGCTGGTGGCGGGATCCGGTGCCGGCGGCGTCACCGGCGCCTACACCGCGGCGCGCGAGG
GCCTCGAGGTGCTGCTCGTCGAGGCGACGTCGAATTCGGTGGCACGACCGCCTATTCGGGCGGCGGCGGCGTCTGGTTCC
CGTGCAATCCGGTGCTGATCCGCGCGGGCACCGACGACACCATCGAGGACGCGCTCACCTACTACCACGCCGTGGTCGGC
GACCGCACCCCGCGCGAGCTGCAGGAAACCTATGTGCGCGGCGGGGCCCCGCTGATCGAATACCTGGAGGCCGACCCGCA
CCTGGCGTTCGTGCCGTTGCCCTGGCCCGACTACTACGGCAAGGCGCCCAAGGCGCGGCTGGACGGCCAACGCCACATCG
CCGCCAAGCCGCTGCCGGTGGCCGCCGCCCCCGAATTCCGGACGCTGATCCGCGGGCCCTTGGACACCGACCGGCTCGGC
GCCGAACCGCCGTCCGACTACTACCTCGGGGGCCGCGCCCTGATTGCGCGATTCCTGCGGGCCATCGGCGAGTTCCCGAC
GGCCACGCTGCGACGCGACACCGCACTGGTTGAGCTCGTGCGCAGCGACGGCCGGGTGGTCGGCGCGGTGGTCGAGACCG
GCGGTGAGCGCCGCGCCGTCCGCGCCCGGCGCGGGGTGCTGCTGGCCGCCGGGGGCTTCGAGGCCAACCCGGACTTGCGC
CGCGCCTACGGGGTGCCGGGGCAGGCGCGAGACACCATGGGCGGCCCGGGAAGTCGCGGCCTGGCGCTGCAGGCGGGCAT
CGCCGTCGGCGCCGACACCGACCTGCTGGACCAGGCGTGGTGGTCGCCGGGCCTGACCCATCCCGACGGCCGCTCGGCGT
TCGCGCTGTGGTTCACCGGCGGCATCTTTGTCAACCAGTCCGGCGACCGGTTCGTCAACGAGTCCATGGCCTACGACCGG
GCCGGCCGGCAGATCCTGGCCCAACTGCGGGACGGGTCGCTCAGGCTGCCGTACTGGATGATCTACGACGACAAGGAGGG
TGCGGTGCCGCCGGTGAAGGCGACCAACGTCTCCCTCGTCGAGACCGAGAAATACGTCGCCGCCGGCTTGTGGCACACCG
CGGACAACCTGCCCGAGCTGGCCGCCAAGATCGGCGTTCCCGCGCAGCGGCTGACGGCAACGGTGTTGCGGTTCAACGGG
TTTGCCGCCGCCGGCGTCGACGAGGACTTCGGCCGGGGCGACGAGGCGTTCGACCGGGCCTTCTCGGCGGGCGCCTCCCC
GCTGGTGCCCATCGACACACCGCCGTATCATGCCGCCGCGTTCGGCGTCTCCGATCTGGGCACCAAGGGCGGTCTGCGCA
CCGACACCGCGGCCCGCGTGCTGGACACCGCCGGCCGGCCCATCCCCGGGCTGTACGCGGCGGGCAACACCATGGCCGCC
CCCAGCGGCACCACCTATCCCGGCGGCGGCAACCCGATCGGAACCAGCATGCTGTTCAGCCATCTGGCCGTGCTGGACAT
GCGCGACCGCCGGCCGTGA

Upstream 100 bases:

>100_bases
GAGTGTTCCGCGGCGGGTTCGGCGGTGTGGTCGGTAACGCTCGTCATGGGAGACGAGCTTAGCGGCGCGGATAGGCTGCG
CTGACTATGGGCACGGTGCA

Downstream 100 bases:

>100_bases
GTTCTAGGCTGGGTCCGGTGACCGACCGAGACCGGCTTCGAGCCCCGTTGGACGCCGCCGCGCTGCGCGCCGAACTGATC
GGCACCGGGCTGGGCTGGCG

Product: 3-ketosteroid-delta-1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MDRGVRRAGGGIRCRRRHRRLHRGARGPRGAARRGDVEFGGTTAYSGGGGVWFPCNPVLIRAGTDDTIEDALTYYHAVVG
DRTPRELQETYVRGGAPLIEYLEADPHLAFVPLPWPDYYGKAPKARLDGQRHIAAKPLPVAAAPEFRTLIRGPLDTDRLG
AEPPSDYYLGGRALIARFLRAIGEFPTATLRRDTALVELVRSDGRVVGAVVETGGERRAVRARRGVLLAAGGFEANPDLR
RAYGVPGQARDTMGGPGSRGLALQAGIAVGADTDLLDQAWWSPGLTHPDGRSAFALWFTGGIFVNQSGDRFVNESMAYDR
AGRQILAQLRDGSLRLPYWMIYDDKEGAVPPVKATNVSLVETEKYVAAGLWHTADNLPELAAKIGVPAQRLTATVLRFNG
FAAAGVDEDFGRGDEAFDRAFSAGASPLVPIDTPPYHAAAFGVSDLGTKGGLRTDTAARVLDTAGRPIPGLYAAGNTMAA
PSGTTYPGGGNPIGTSMLFSHLAVLDMRDRRP

Sequences:

>Translated_512_residues
MDRGVRRAGGGIRCRRRHRRLHRGARGPRGAARRGDVEFGGTTAYSGGGGVWFPCNPVLIRAGTDDTIEDALTYYHAVVG
DRTPRELQETYVRGGAPLIEYLEADPHLAFVPLPWPDYYGKAPKARLDGQRHIAAKPLPVAAAPEFRTLIRGPLDTDRLG
AEPPSDYYLGGRALIARFLRAIGEFPTATLRRDTALVELVRSDGRVVGAVVETGGERRAVRARRGVLLAAGGFEANPDLR
RAYGVPGQARDTMGGPGSRGLALQAGIAVGADTDLLDQAWWSPGLTHPDGRSAFALWFTGGIFVNQSGDRFVNESMAYDR
AGRQILAQLRDGSLRLPYWMIYDDKEGAVPPVKATNVSLVETEKYVAAGLWHTADNLPELAAKIGVPAQRLTATVLRFNG
FAAAGVDEDFGRGDEAFDRAFSAGASPLVPIDTPPYHAAAFGVSDLGTKGGLRTDTAARVLDTAGRPIPGLYAAGNTMAA
PSGTTYPGGGNPIGTSMLFSHLAVLDMRDRRP
>Mature_512_residues
MDRGVRRAGGGIRCRRRHRRLHRGARGPRGAARRGDVEFGGTTAYSGGGGVWFPCNPVLIRAGTDDTIEDALTYYHAVVG
DRTPRELQETYVRGGAPLIEYLEADPHLAFVPLPWPDYYGKAPKARLDGQRHIAAKPLPVAAAPEFRTLIRGPLDTDRLG
AEPPSDYYLGGRALIARFLRAIGEFPTATLRRDTALVELVRSDGRVVGAVVETGGERRAVRARRGVLLAAGGFEANPDLR
RAYGVPGQARDTMGGPGSRGLALQAGIAVGADTDLLDQAWWSPGLTHPDGRSAFALWFTGGIFVNQSGDRFVNESMAYDR
AGRQILAQLRDGSLRLPYWMIYDDKEGAVPPVKATNVSLVETEKYVAAGLWHTADNLPELAAKIGVPAQRLTATVLRFNG
FAAAGVDEDFGRGDEAFDRAFSAGASPLVPIDTPPYHAAAFGVSDLGTKGGLRTDTAARVLDTAGRPIPGLYAAGNTMAA
PSGTTYPGGGNPIGTSMLFSHLAVLDMRDRRP

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 54760; Mature: 54760

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRGVRRAGGGIRCRRRHRRLHRGARGPRGAARRGDVEFGGTTAYSGGGGVWFPCNPVLI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCEEE
RAGTDDTIEDALTYYHAVVGDRTPRELQETYVRGGAPLIEYLEADPHLAFVPLPWPDYYG
ECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCHHHCC
KAPKARLDGQRHIAAKPLPVAAAPEFRTLIRGPLDTDRLGAEPPSDYYLGGRALIARFLR
CCCCHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHCCCCCCCCCEECHHHHHHHHHH
AIGEFPTATLRRDTALVELVRSDGRVVGAVVETGGERRAVRARRGVLLAAGGFEANPDLR
HHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCCCCCHH
RAYGVPGQARDTMGGPGSRGLALQAGIAVGADTDLLDQAWWSPGLTHPDGRSAFALWFTG
HHCCCCCCCCCCCCCCCCCCEEEEECEEECCCCHHHHHHHCCCCCCCCCCCCEEEEEEEC
GIFVNQSGDRFVNESMAYDRAGRQILAQLRDGSLRLPYWMIYDDKEGAVPPVKATNVSLV
CEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCCCCCCCCCCEEEE
ETEKYVAAGLWHTADNLPELAAKIGVPAQRLTATVLRFNGFAAAGVDEDFGRGDEAFDRA
EHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHCCCCHHHHHHH
FSAGASPLVPIDTPPYHAAAFGVSDLGTKGGLRTDTAARVLDTAGRPIPGLYAAGNTMAA
HHCCCCCEEECCCCCCHHHHCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCEEEC
PSGTTYPGGGNPIGTSMLFSHLAVLDMRDRRP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDRGVRRAGGGIRCRRRHRRLHRGARGPRGAARRGDVEFGGTTAYSGGGGVWFPCNPVLI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCEEE
RAGTDDTIEDALTYYHAVVGDRTPRELQETYVRGGAPLIEYLEADPHLAFVPLPWPDYYG
ECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCHHHCC
KAPKARLDGQRHIAAKPLPVAAAPEFRTLIRGPLDTDRLGAEPPSDYYLGGRALIARFLR
CCCCHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHCCCCCCCCCEECHHHHHHHHHH
AIGEFPTATLRRDTALVELVRSDGRVVGAVVETGGERRAVRARRGVLLAAGGFEANPDLR
HHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCCCCCHH
RAYGVPGQARDTMGGPGSRGLALQAGIAVGADTDLLDQAWWSPGLTHPDGRSAFALWFTG
HHCCCCCCCCCCCCCCCCCCEEEEECEEECCCCHHHHHHHCCCCCCCCCCCCEEEEEEEC
GIFVNQSGDRFVNESMAYDRAGRQILAQLRDGSLRLPYWMIYDDKEGAVPPVKATNVSLV
CEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCCCCCCCCCCEEEE
ETEKYVAAGLWHTADNLPELAAKIGVPAQRLTATVLRFNGFAAAGVDEDFGRGDEAFDRA
EHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHCCCCHHHHHHH
FSAGASPLVPIDTPPYHAAAFGVSDLGTKGGLRTDTAARVLDTAGRPIPGLYAAGNTMAA
HHCCCCCEEECCCCCCHHHHCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCEEEC
PSGTTYPGGGNPIGTSMLFSHLAVLDMRDRRP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]