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The map label for this gene is birA

Identifier: 41409495

GI number: 41409495

Start: 3772658

End: 3773503

Strand: Reverse

Name: birA

Synonym: MAP3397c

Alternate gene names: 41409495

Gene position: 3773503-3772658 (Counterclockwise)

Preceding gene: 41409496

Following gene: 41409494

Centisome position: 78.13

GC content: 76.12

Gene sequence:

>846_bases
GTGAGTTCTAGGCTGGGTCCGGTGACCGACCGAGACCGGCTTCGAGCCCCGTTGGACGCCGCCGCGCTGCGCGCCGAACT
GATCGGCACCGGGCTGGGCTGGCGCCGGCTCGACGTCGTCGAGCAGACCGGCTCCACCAACGCCGACCTGCTGGCGCGCG
CGGCGGCGGGCGACGACGTCGCCGGGGCGGTGCTGATCGCCGAGCACCAGACCGCCGGGCGGGGGCGGCACGGCCGGGGC
TGGTCGGCCACGCCGCGCGCCCAGGTCACCATGTCGGTCGGGGTCAGCGTCGTCGACGTCCCGGCCGCCGGGTGGGGCTG
GCTGCCGCTGGCCACCGGGGTGGCCGTGGTCGACACGGTGGCCCCGTTGCTGCAGCCGGCGGGGGTGGCGGTCGGGCTGA
AGTGGCCCAACGACGTGCTGGCCGGGCCGGCCGGCACGCCGGGCAAGCTGGCCGGAATCCTCGCCGAGGTGTCCAAGCCG
GTGGTGGTTATCGGCTTGGGGCTCAACGTGACTCAGTCCGCCGACGAGATCGACGGCCCCGGCGCCACCTCGCTGCTCGA
CCTCGGGGTGGCCGCGCCCGACCGCACCGCGCTGGTGTCCGGGCTGTTGCGCGAGCTCGGCCGGCGCATCGTCAGCTGGC
AGGCCGCGCGCGGGGCGGACTGGGCGCTGGCCGCCGACTACCGGGCCCGCAGCCTGACCATCGGCACCCGGGTGCGCGCC
GACTTGCCCGGCGGCAAACACGTCGAGGGCACCGCCAGCGGCATCGACGACCAGGGCCGGCTGTGTCTGGACAGCGGCGG
GCAAACCGTCGTCGTCGCCGCCGGCGACGTGGTGCATCTGCGCTAG

Upstream 100 bases:

>100_bases
CCGCCCCCAGCGGCACCACCTATCCCGGCGGCGGCAACCCGATCGGAACCAGCATGCTGTTCAGCCATCTGGCCGTGCTG
GACATGCGCGACCGCCGGCC

Downstream 100 bases:

>100_bases
CCGCACCCGCCCGGCACCGGGCCCGGCTAAGGTCGGCGGGCATGGGCTACCCGGATAACGTTCTGGCCGCCGGCGAACAC
GTCATCGTGCACCGCCACCC

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSSRLGPVTDRDRLRAPLDAAALRAELIGTGLGWRRLDVVEQTGSTNADLLARAAAGDDVAGAVLIAEHQTAGRGRHGRG
WSATPRAQVTMSVGVSVVDVPAAGWGWLPLATGVAVVDTVAPLLQPAGVAVGLKWPNDVLAGPAGTPGKLAGILAEVSKP
VVVIGLGLNVTQSADEIDGPGATSLLDLGVAAPDRTALVSGLLRELGRRIVSWQAARGADWALAADYRARSLTIGTRVRA
DLPGGKHVEGTASGIDDQGRLCLDSGGQTVVVAAGDVVHLR

Sequences:

>Translated_281_residues
MSSRLGPVTDRDRLRAPLDAAALRAELIGTGLGWRRLDVVEQTGSTNADLLARAAAGDDVAGAVLIAEHQTAGRGRHGRG
WSATPRAQVTMSVGVSVVDVPAAGWGWLPLATGVAVVDTVAPLLQPAGVAVGLKWPNDVLAGPAGTPGKLAGILAEVSKP
VVVIGLGLNVTQSADEIDGPGATSLLDLGVAAPDRTALVSGLLRELGRRIVSWQAARGADWALAADYRARSLTIGTRVRA
DLPGGKHVEGTASGIDDQGRLCLDSGGQTVVVAAGDVVHLR
>Mature_280_residues
SSRLGPVTDRDRLRAPLDAAALRAELIGTGLGWRRLDVVEQTGSTNADLLARAAAGDDVAGAVLIAEHQTAGRGRHGRGW
SATPRAQVTMSVGVSVVDVPAAGWGWLPLATGVAVVDTVAPLLQPAGVAVGLKWPNDVLAGPAGTPGKLAGILAEVSKPV
VVIGLGLNVTQSADEIDGPGATSLLDLGVAAPDRTALVSGLLRELGRRIVSWQAARGADWALAADYRARSLTIGTRVRAD
LPGGKHVEGTASGIDDQGRLCLDSGGQTVVVAAGDVVHLR

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=234, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28625; Mature: 28494

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRLGPVTDRDRLRAPLDAAALRAELIGTGLGWRRLDVVEQTGSTNADLLARAAAGDDV
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHEECCCCCCC
AGAVLIAEHQTAGRGRHGRGWSATPRAQVTMSVGVSVVDVPAAGWGWLPLATGVAVVDTV
CEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHH
APLLQPAGVAVGLKWPNDVLAGPAGTPGKLAGILAEVSKPVVVIGLGLNVTQSADEIDGP
HHHHCCCCEEEEECCCCHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHCCCC
GATSLLDLGVAAPDRTALVSGLLRELGRRIVSWQAARGADWALAADYRARSLTIGTRVRA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCEEEE
DLPGGKHVEGTASGIDDQGRLCLDSGGQTVVVAAGDVVHLR
ECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCEEEEC
>Mature Secondary Structure 
SSRLGPVTDRDRLRAPLDAAALRAELIGTGLGWRRLDVVEQTGSTNADLLARAAAGDDV
CCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHEECCCCCCC
AGAVLIAEHQTAGRGRHGRGWSATPRAQVTMSVGVSVVDVPAAGWGWLPLATGVAVVDTV
CEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHH
APLLQPAGVAVGLKWPNDVLAGPAGTPGKLAGILAEVSKPVVVIGLGLNVTQSADEIDGP
HHHHCCCCEEEEECCCCHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHCCCC
GATSLLDLGVAAPDRTALVSGLLRELGRRIVSWQAARGADWALAADYRARSLTIGTRVRA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCEEEE
DLPGGKHVEGTASGIDDQGRLCLDSGGQTVVVAAGDVVHLR
ECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]