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The map label for this gene is eco57IBM [H]

Identifier: 41409474

GI number: 41409474

Start: 3749429

End: 3751009

Strand: Direct

Name: eco57IBM [H]

Synonym: MAP3376

Alternate gene names: 41409474

Gene position: 3749429-3751009 (Clockwise)

Preceding gene: 41409473

Following gene: 41409475

Centisome position: 77.63

GC content: 73.88

Gene sequence:

>1581_bases
GTGACGACGTTCGCGGGCAAGGCGGCGGCCTCGGCCGACAAGGTCCGCGGCGGCTACTACACGCCGGCGCCGGTGGCGCG
GTTCCTGGCCCACTGGGTGCGCCGGGCCGGGCCGAGGATCGTCGAACCCTCCTGCGGCGACGGCGCGATCCTGCGCGAGC
TGGCCCGGCTCAGCGACCGGGTGCACGGCGTCGAGCTGATCCCGCACGAGGCGGCCAAGGCCCGGCGGTTCGCACCGGTG
TCCGCCGAGAGCCTGTTCAGCTGGCTCGCCACCGCCGAGGACGGCGGCTGGGACGGCGTCGCCGGCAATCCGCCCTACAT
CCGGTTCGGCAATTGGGCGTCGCCGCAACGCGATCCGGCGCTGGCGTTGCTGCGGCGCGAGGGTTTACGGCCGACCCGGC
TGACCAACGCCTGGGTGCCGTTCGTGGTGGCCGGCACCGTGCTGGTGCGCGACGGCGGCCGGGTGGGGCTGGTGCTTCCG
GCCGAGCTGCTGCAGGTCGGCTACGCCGCGCAGCTGCGCGAGTTCCTGCTGAGCCGATTCCGCGACATCACGCTGCTGAC
CTTCGAGCGGCTGGTGTTCGACGGCGTCCTGCAGGAGGTGGTGTTGTTCTGCGGCGTGGTCGGGACGGGTCCGGCGCGCA
TCCGCACCGTGACCCTGGCCGGCGCGGACGCCCTGGCGGACATCGACGTCGAGCGCCTGGCGTCCGCACCCGCGCTGCTG
CACGAGCACGAGAAGTGGACCAAGTACTTTCTGGACCCGGCCGCGATCGAGCTGCTGCGCGCGCTCAAGGCCTCGGCGAC
GCTGACCCGGCTGGGCGCGCTCGCCGAGGTGGACGTGGGCATCGTGACCGGCCGCAACGCCTTTTTCACCTTCACCGACG
AGCAGGTGACCGCGTTGGGGCTGCGCCGGCACTGCGTGCCCCTCGTCTCGCGCAGCGCCCAGCTGTCCGGGCTGATCTAC
GACACCGATTGCCGGGCAAGCGATCTCGCCGCCCGGCAGCGCGGCTGGCTGCTCAACGCGCCGCGCGAGCCGGCCGATCC
CGCGTTGACCGCGCACATCCGCGCCGGGGAGGCGGCCGGGGTGCACCGCGGCTACAAGTGCTCGATCCGCACGCCGTGGT
GGAGCACGCCGTCGCTGTGGCAGCCCGACCTGTTCCTGCTGCGCCAGATCCACGCCGCGCCCCGGCTGACGGTCAACGCC
GCGGGGCCCACCAGCACCGACACCGTGCACCGGGTGCGGGTTGGGCCGGGCGTCGACCCGGCGGCGCTGGCCGCGGTGTT
CCACAACAGCGTCACGTTCGCGTTCGCCGAGATCATGGGCCGCAGCTACGGCGGGGGCGTGCTGGAGCTGGAGCCGCGCG
AGGCCGAGCAGCTGCCGCTCCCCCGGCCGGAGTGCGCGGACGCCGATCTGGTCGGCGATGTCGACCTGTTGCTGAAGGCC
GGCGAGCTGGACAAGGCGCTGGACGTCGTGGACCGCCGGGTGCTGATCGACGCGCTGGGCGTCCCGCCCCGGGCGGTGGC
GGACTGCCGCGCGGCCTGGGCGTGTCTGCGGGATCGCCGGAAACGGCGGGCATCCCGGTGA

Upstream 100 bases:

>100_bases
CCGTTGGGGGCCATCGCCATCGGCTATGCCGCCGAACCCGCCGGGCCGCGGGGTCCGGCGGACCCTGGGGATCTGCTGAT
CCGCAAGTGATACTGAGCGC

Downstream 100 bases:

>100_bases
GCGTGCGCGACTCGGTGCTGGCGCTGCTGACGCAGTGGGATCCGCCCGATGCGGCCCAGGATTCGTTGCGGCACGCGGTG
TTGGCGTTCGTGCACGCCCG

Product: hypothetical protein

Products: NA

Alternate protein names: M.Eco57IB; Adenine-specific methyltransferase Eco57IB [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTTFAGKAAASADKVRGGYYTPAPVARFLAHWVRRAGPRIVEPSCGDGAILRELARLSDRVHGVELIPHEAAKARRFAPV
SAESLFSWLATAEDGGWDGVAGNPPYIRFGNWASPQRDPALALLRREGLRPTRLTNAWVPFVVAGTVLVRDGGRVGLVLP
AELLQVGYAAQLREFLLSRFRDITLLTFERLVFDGVLQEVVLFCGVVGTGPARIRTVTLAGADALADIDVERLASAPALL
HEHEKWTKYFLDPAAIELLRALKASATLTRLGALAEVDVGIVTGRNAFFTFTDEQVTALGLRRHCVPLVSRSAQLSGLIY
DTDCRASDLAARQRGWLLNAPREPADPALTAHIRAGEAAGVHRGYKCSIRTPWWSTPSLWQPDLFLLRQIHAAPRLTVNA
AGPTSTDTVHRVRVGPGVDPAALAAVFHNSVTFAFAEIMGRSYGGGVLELEPREAEQLPLPRPECADADLVGDVDLLLKA
GELDKALDVVDRRVLIDALGVPPRAVADCRAAWACLRDRRKRRASR

Sequences:

>Translated_526_residues
MTTFAGKAAASADKVRGGYYTPAPVARFLAHWVRRAGPRIVEPSCGDGAILRELARLSDRVHGVELIPHEAAKARRFAPV
SAESLFSWLATAEDGGWDGVAGNPPYIRFGNWASPQRDPALALLRREGLRPTRLTNAWVPFVVAGTVLVRDGGRVGLVLP
AELLQVGYAAQLREFLLSRFRDITLLTFERLVFDGVLQEVVLFCGVVGTGPARIRTVTLAGADALADIDVERLASAPALL
HEHEKWTKYFLDPAAIELLRALKASATLTRLGALAEVDVGIVTGRNAFFTFTDEQVTALGLRRHCVPLVSRSAQLSGLIY
DTDCRASDLAARQRGWLLNAPREPADPALTAHIRAGEAAGVHRGYKCSIRTPWWSTPSLWQPDLFLLRQIHAAPRLTVNA
AGPTSTDTVHRVRVGPGVDPAALAAVFHNSVTFAFAEIMGRSYGGGVLELEPREAEQLPLPRPECADADLVGDVDLLLKA
GELDKALDVVDRRVLIDALGVPPRAVADCRAAWACLRDRRKRRASR
>Mature_525_residues
TTFAGKAAASADKVRGGYYTPAPVARFLAHWVRRAGPRIVEPSCGDGAILRELARLSDRVHGVELIPHEAAKARRFAPVS
AESLFSWLATAEDGGWDGVAGNPPYIRFGNWASPQRDPALALLRREGLRPTRLTNAWVPFVVAGTVLVRDGGRVGLVLPA
ELLQVGYAAQLREFLLSRFRDITLLTFERLVFDGVLQEVVLFCGVVGTGPARIRTVTLAGADALADIDVERLASAPALLH
EHEKWTKYFLDPAAIELLRALKASATLTRLGALAEVDVGIVTGRNAFFTFTDEQVTALGLRRHCVPLVSRSAQLSGLIYD
TDCRASDLAARQRGWLLNAPREPADPALTAHIRAGEAAGVHRGYKCSIRTPWWSTPSLWQPDLFLLRQIHAAPRLTVNAA
GPTSTDTVHRVRVGPGVDPAALAAVFHNSVTFAFAEIMGRSYGGGVLELEPREAEQLPLPRPECADADLVGDVDLLLKAG
ELDKALDVVDRRVLIDALGVPPRAVADCRAAWACLRDRRKRRASR

Specific function: This methylase recognizes the double-stranded sequence 5'-CTGAAG-3' in one strand and 5'-CTTCAG-3' in the other, causes specific methylation on A-5 on one strand, and protects the DNA from cleavage by the Eco57I endonuclease [H]

COG id: COG0827

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 57103; Mature: 56972

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFAGKAAASADKVRGGYYTPAPVARFLAHWVRRAGPRIVEPSCGDGAILRELARLSDR
CCCCCCCHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHH
VHGVELIPHEAAKARRFAPVSAESLFSWLATAEDGGWDGVAGNPPYIRFGNWASPQRDPA
CCCEEECCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHH
LALLRREGLRPTRLTNAWVPFVVAGTVLVRDGGRVGLVLPAELLQVGYAAQLREFLLSRF
HHHHHHCCCCCCHHCCCCHHHHHCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHH
RDITLLTFERLVFDGVLQEVVLFCGVVGTGPARIRTVTLAGADALADIDVERLASAPALL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHCCHHHHHCCHHHH
HEHEKWTKYFLDPAAIELLRALKASATLTRLGALAEVDVGIVTGRNAFFTFTDEQVTALG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHHH
LRRHCVPLVSRSAQLSGLIYDTDCRASDLAARQRGWLLNAPREPADPALTAHIRAGEAAG
HHHHHHHHHHCCHHHCEEEEECCCCHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCCC
VHRGYKCSIRTPWWSTPSLWQPDLFLLRQIHAAPRLTVNAAGPTSTDTVHRVRVGPGVDP
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCH
AALAAVFHNSVTFAFAEIMGRSYGGGVLELEPREAEQLPLPRPECADADLVGDVDLLLKA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHCCCCCCCCCCCCCCCCCHHHHHCC
GELDKALDVVDRRVLIDALGVPPRAVADCRAAWACLRDRRKRRASR
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTFAGKAAASADKVRGGYYTPAPVARFLAHWVRRAGPRIVEPSCGDGAILRELARLSDR
CCCCCCHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHH
VHGVELIPHEAAKARRFAPVSAESLFSWLATAEDGGWDGVAGNPPYIRFGNWASPQRDPA
CCCEEECCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHH
LALLRREGLRPTRLTNAWVPFVVAGTVLVRDGGRVGLVLPAELLQVGYAAQLREFLLSRF
HHHHHHCCCCCCHHCCCCHHHHHCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHH
RDITLLTFERLVFDGVLQEVVLFCGVVGTGPARIRTVTLAGADALADIDVERLASAPALL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHCCHHHHHCCHHHH
HEHEKWTKYFLDPAAIELLRALKASATLTRLGALAEVDVGIVTGRNAFFTFTDEQVTALG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHHH
LRRHCVPLVSRSAQLSGLIYDTDCRASDLAARQRGWLLNAPREPADPALTAHIRAGEAAG
HHHHHHHHHHCCHHHCEEEEECCCCHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCCC
VHRGYKCSIRTPWWSTPSLWQPDLFLLRQIHAAPRLTVNAAGPTSTDTVHRVRVGPGVDP
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCH
AALAAVFHNSVTFAFAEIMGRSYGGGVLELEPREAEQLPLPRPECADADLVGDVDLLLKA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHCCCCCCCCCCCCCCCCCHHHHHCC
GELDKALDVVDRRVLIDALGVPPRAVADCRAAWACLRDRRKRRASR
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1334261; 1334260 [H]