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The map label for this gene is cofE [H]

Identifier: 41409473

GI number: 41409473

Start: 3748069

End: 3749418

Strand: Direct

Name: cofE [H]

Synonym: MAP3375

Alternate gene names: 41409473

Gene position: 3748069-3749418 (Clockwise)

Preceding gene: 41409472

Following gene: 41409474

Centisome position: 77.6

GC content: 74.81

Gene sequence:

>1350_bases
GTGAGTCCCGCCGGCGAGCACGGCACCGCCGCCCCGATCGAGATCCTGCCGGTGGCCGGGCTGCCCGAATTCCGCCCCGG
CGACGATCTGGGGGCGGCCGTCGCCAAGGCCGCGCCGTGGCTGCGCGACGGCGACGTCGTCGTCGTCACCAGCAAGGCCG
TCTCCAAGTGCGAGGGCCGGCTGGTGCCGGCGCCGGCCGATCCCGAGGAACGCGATCGGCTGCGGCGCAAGCTGGTCGAC
GAGGAAGCCGTCCGGGTGCTGGCCCGCAAGGGCCGCACCCTGATTACCGAGAACCGGCACGGGCTGGTGCAGGCCGCCGC
CGGGGTAGACGGATCCAACGTCGGCCGCGACGAGCTGGCGCTGCTGCCGCTGGACCCCGACGCCAGCGCCGCGGCGCTGC
GCGCGCGGCTGCGCGAGCTACTGGGCGTCGAGGTCGCCGTGCTGGTCACCGACACCATGGGCCGCGCCTGGCGCAACGGC
CAGACCGACGCCGCGGTGGGGGCGGCGGGACTGGCTGTGCTGCACGGCTATTCGGGTGCGGTGGATCAGCACGGCAACGA
GCTGCTGGTCACCGAGGTGGCGATCGCCGACGAGATCGCCGCGGCCGCCGATCTGGTGAAGGGCAAACTGACCGCGATGC
CGGTGGCGGTGGTGCGCGGGCTGTCGGTGACCGACGACGGGTCGACCGCCCGGCAGTTGTTGCGGCCGGGCACCGAGGAC
CTGTTCTGGCTGGGCACCGCCGAGGCCATCGAGTTGGGCCGCCGCCAGGCGCAGCTGCTGCGCCGTTCGGTGCGCCGGTT
CAGCGCCGAGCCGGTGCCCGCGGAGCTGGTGCGCGAGGCCGTCGCCGAGGCCCTGACCGCGCCGGCCCCGCACCACACCC
GCCCGGTGCGGTTCGTCTGGCTGCAGACCCCGGCGGTGCGGACCCGGCTGCTGGACGCGATGAAGGACAAGTGGCGCGCC
GACCTGGCCGGCGACGGCCGGCCCGCCGAGTCGATCGAACGCCGGGTGGCGCGCGGCCAGATCCTCTACGACGCGCCCGA
AGTCGTCATCCCGATGCTGGTCCCCGACGGCGCGCACAGCTATCCCGACGCCGCCCGCACCGACGCCGAGCACACCATGT
TCACCGTGGCGGTCGGCGCGGCCGTGCAGGCCCTGCTGGTCGGGTTGGCGGTGCGCGGCCTGGGCAGCTGCTGGATCGGC
TCCACGATCTTCGCCGCCGACCTGGTGCGCACCGAGCTCGGGTTGCCCGCCGACTGGGAGCCGTTGGGGGCCATCGCCAT
CGGCTATGCCGCCGAACCCGCCGGGCCGCGGGGTCCGGCGGACCCTGGGGATCTGCTGATCCGCAAGTGA

Upstream 100 bases:

>100_bases
TCGAGGGGGTGGCGGTGCGCGCGGTGCCGCTGATGATGACCGACCCCGCGGCGACCGCCGAGATGGTCAGCGCGGGGCTG
CAGCTGGCGGGCGTGACGCC

Downstream 100 bases:

>100_bases
TACTGAGCGCGTGACGACGTTCGCGGGCAAGGCGGCGGCCTCGGCCGACAAGGTCCGCGGCGGCTACTACACGCCGGCGC
CGGTGGCGCGGTTCCTGGCC

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSPAGEHGTAAPIEILPVAGLPEFRPGDDLGAAVAKAAPWLRDGDVVVVTSKAVSKCEGRLVPAPADPEERDRLRRKLVD
EEAVRVLARKGRTLITENRHGLVQAAAGVDGSNVGRDELALLPLDPDASAAALRARLRELLGVEVAVLVTDTMGRAWRNG
QTDAAVGAAGLAVLHGYSGAVDQHGNELLVTEVAIADEIAAAADLVKGKLTAMPVAVVRGLSVTDDGSTARQLLRPGTED
LFWLGTAEAIELGRRQAQLLRRSVRRFSAEPVPAELVREAVAEALTAPAPHHTRPVRFVWLQTPAVRTRLLDAMKDKWRA
DLAGDGRPAESIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVGLAVRGLGSCWIG
STIFAADLVRTELGLPADWEPLGAIAIGYAAEPAGPRGPADPGDLLIRK

Sequences:

>Translated_449_residues
MSPAGEHGTAAPIEILPVAGLPEFRPGDDLGAAVAKAAPWLRDGDVVVVTSKAVSKCEGRLVPAPADPEERDRLRRKLVD
EEAVRVLARKGRTLITENRHGLVQAAAGVDGSNVGRDELALLPLDPDASAAALRARLRELLGVEVAVLVTDTMGRAWRNG
QTDAAVGAAGLAVLHGYSGAVDQHGNELLVTEVAIADEIAAAADLVKGKLTAMPVAVVRGLSVTDDGSTARQLLRPGTED
LFWLGTAEAIELGRRQAQLLRRSVRRFSAEPVPAELVREAVAEALTAPAPHHTRPVRFVWLQTPAVRTRLLDAMKDKWRA
DLAGDGRPAESIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVGLAVRGLGSCWIG
STIFAADLVRTELGLPADWEPLGAIAIGYAAEPAGPRGPADPGDLLIRK
>Mature_448_residues
SPAGEHGTAAPIEILPVAGLPEFRPGDDLGAAVAKAAPWLRDGDVVVVTSKAVSKCEGRLVPAPADPEERDRLRRKLVDE
EAVRVLARKGRTLITENRHGLVQAAAGVDGSNVGRDELALLPLDPDASAAALRARLRELLGVEVAVLVTDTMGRAWRNGQ
TDAAVGAAGLAVLHGYSGAVDQHGNELLVTEVAIADEIAAAADLVKGKLTAMPVAVVRGLSVTDDGSTARQLLRPGTEDL
FWLGTAEAIELGRRQAQLLRRSVRRFSAEPVPAELVREAVAEALTAPAPHHTRPVRFVWLQTPAVRTRLLDAMKDKWRAD
LAGDGRPAESIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVGLAVRGLGSCWIGS
TIFAADLVRTELGLPADWEPLGAIAIGYAAEPAGPRGPADPGDLLIRK

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225
- InterPro:   IPR019943
- InterPro:   IPR000415 [H]

Pfam domain/function: PF01996 F420_ligase; PF00881 Nitroreductase [H]

EC number: NA

Molecular weight: Translated: 47399; Mature: 47267

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAGEHGTAAPIEILPVAGLPEFRPGDDLGAAVAKAAPWLRDGDVVVVTSKAVSKCEGR
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCC
LVPAPADPEERDRLRRKLVDEEAVRVLARKGRTLITENRHGLVQAAAGVDGSNVGRDELA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCEE
LLPLDPDASAAALRARLRELLGVEVAVLVTDTMGRAWRNGQTDAAVGAAGLAVLHGYSGA
EEECCCCCHHHHHHHHHHHHHCCEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
VDQHGNELLVTEVAIADEIAAAADLVKGKLTAMPVAVVRGLSVTDDGSTARQLLRPGTED
HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCC
LFWLGTAEAIELGRRQAQLLRRSVRRFSAEPVPAELVREAVAEALTAPAPHHTRPVRFVW
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEE
LQTPAVRTRLLDAMKDKWRADLAGDGRPAESIERRVARGQILYDAPEVVIPMLVPDGAHS
EECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCCCC
YPDAARTDAEHTMFTVAVGAAVQALLVGLAVRGLGSCWIGSTIFAADLVRTELGLPADWE
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
PLGAIAIGYAAEPAGPRGPADPGDLLIRK
CCCCEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SPAGEHGTAAPIEILPVAGLPEFRPGDDLGAAVAKAAPWLRDGDVVVVTSKAVSKCEGR
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCC
LVPAPADPEERDRLRRKLVDEEAVRVLARKGRTLITENRHGLVQAAAGVDGSNVGRDELA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCEE
LLPLDPDASAAALRARLRELLGVEVAVLVTDTMGRAWRNGQTDAAVGAAGLAVLHGYSGA
EEECCCCCHHHHHHHHHHHHHCCEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
VDQHGNELLVTEVAIADEIAAAADLVKGKLTAMPVAVVRGLSVTDDGSTARQLLRPGTED
HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCC
LFWLGTAEAIELGRRQAQLLRRSVRRFSAEPVPAELVREAVAEALTAPAPHHTRPVRFVW
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEE
LQTPAVRTRLLDAMKDKWRADLAGDGRPAESIERRVARGQILYDAPEVVIPMLVPDGAHS
EECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCCCC
YPDAARTDAEHTMFTVAVGAAVQALLVGLAVRGLGSCWIGSTIFAADLVRTELGLPADWE
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
PLGAIAIGYAAEPAGPRGPADPGDLLIRK
CCCCEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA