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The map label for this gene is tgs3 [H]

Identifier: 41409445

GI number: 41409445

Start: 3715674

End: 3717095

Strand: Reverse

Name: tgs3 [H]

Synonym: MAP3347c

Alternate gene names: 41409445

Gene position: 3717095-3715674 (Counterclockwise)

Preceding gene: 41409447

Following gene: 41409444

Centisome position: 76.96

GC content: 69.83

Gene sequence:

>1422_bases
ATGGTTAGCCGGTTGTCCCCGGCGGATGCGTCGTTCTACCGGCTGGAGAACACCGCCACCCCCATGTACGTCGGATCGCT
GGCGATCCTGCGCCGCCCGCGTGCCGGGCTGAGCTACGAGACGCTGCTGCACACCATCGAGCAGCGGCTGCCGCAGATAC
CGCGCTACCGGCAGAAGGTGCGGGAGGTGCCGGTCGGCACCGCCCGGCCGGTGTGGATCGACGACGGCGACTTCGACATC
ACCTATCACGTGCGCCGCTCGGCGCTGCCGTCGCCGGGCAGTGACGAGCAGCTGCACGAGCTGATCGCCCGGCTGGCCGC
CCGGCCGCTGGACAAGACGCGGCCGCTGTGGGAGATGTATCTGGTCGAGGGCCTGTCCAAGAACCGGCTGGCGCTCTACA
CCAAGTCCCATCAAGCGCTGATCAACGGGATGACGGCGCTGGAGATCAACCACGTCATCGCCGATCGGACCAAACGCCCG
CCGGCCTTCCCCGAAGACATCTGGGTCCCGGAACGCGACCCGGGCAACACCCGGCTGGTCCTCGGCGCGATCGGGGAGTG
GCTGGTGGGCCCCAGCGCGCAGCTGCAGGCCGTCGGGTCGGCGATCGGCGGGCTGGTGACCAACTACGGCGAGCTGGTCG
ACGCCGGCCGGCGGGTCTTCGATTTCGTGCGCAACGTGGCCCGCGGCACCGCGCCCAGCAGCCCGCTCAACGCCACCGTG
TCGCGCCACCGGCGGTTCACCGTGGCCAGCGGCAGCCTGGAGGACTACCGCACCGTGCGGGCCCGCTACGACTGCGACGT
CAACGACGTGGTGCTGGCGGTGATCGCCGGCGCCCTGGGCAGCTGGCTGATGTCCCGCGGGGTGGCGGTGTCGCCCACCG
CGACGGTGCGGGCGATGGCGCCGCTGTCGGTGTACGCCGACGGCGACCTGGACGTCAGCGGCCCCGGTCAGGCGATCAGC
CAGGTGATGCCGTTTCTGGTCGACCTACCGGTGGGGGAGCCGAACGCGGTGGTGCGGCTCTCCCAGATCGCGCACGCCAC
CGAATCCAACCCGACGGCCGCGCATCTGGTGGACGCCAGGACCATCGTCACGCTGTCGGGTTTCGCCCCGCCCACCCTGC
ACGCCATGGGCATCCGGGTCGCGACCAGCTTCCCGAGCTTCTCCAAGCGCACCTTCAACCTGTTGATCACCAACGCGCCC
GGGGCGCAGTCGCAGATGTACGTCGCCGGCACCAAGCTGCTGGAGACCTACTCGGTGCCGCCGCTGCTGCACAACCAGGC
GCTGGCCATCGGCGTGACGTCCTACAACGGCATGCTCTACTACGGCATCAACGCCGACCGCGAAGCGATGAGCGACGTCG
ACCTGCTGCCCGCATTGCTGAACCAGGCGCTCGAGGAACTGGTGGAGGCCGCCCGGTCTTAG

Upstream 100 bases:

>100_bases
GTACCCGCGCTGACGGGCGCGCCGGCCGGACCACTACGGTGGGCGAGAAATACGGTCGCTGCGCGCACGGACGACCCACT
GACCAGGAGGACAGCGCTGC

Downstream 100 bases:

>100_bases
GCAGCGCTAGACAGCGCTGAAAATCCCTGCGTGGGGCTATCATTCGTGGCTGTGAGCAGCGCGAAAAATGACGGCGTTCC
CCTCAAGGCCGGCAAGAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: TGS3; Probable triacylglycerol synthase tgs3 [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MVSRLSPADASFYRLENTATPMYVGSLAILRRPRAGLSYETLLHTIEQRLPQIPRYRQKVREVPVGTARPVWIDDGDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKTRPLWEMYLVEGLSKNRLALYTKSHQALINGMTALEINHVIADRTKRP
PAFPEDIWVPERDPGNTRLVLGAIGEWLVGPSAQLQAVGSAIGGLVTNYGELVDAGRRVFDFVRNVARGTAPSSPLNATV
SRHRRFTVASGSLEDYRTVRARYDCDVNDVVLAVIAGALGSWLMSRGVAVSPTATVRAMAPLSVYADGDLDVSGPGQAIS
QVMPFLVDLPVGEPNAVVRLSQIAHATESNPTAAHLVDARTIVTLSGFAPPTLHAMGIRVATSFPSFSKRTFNLLITNAP
GAQSQMYVAGTKLLETYSVPPLLHNQALAIGVTSYNGMLYYGINADREAMSDVDLLPALLNQALEELVEAARS

Sequences:

>Translated_473_residues
MVSRLSPADASFYRLENTATPMYVGSLAILRRPRAGLSYETLLHTIEQRLPQIPRYRQKVREVPVGTARPVWIDDGDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKTRPLWEMYLVEGLSKNRLALYTKSHQALINGMTALEINHVIADRTKRP
PAFPEDIWVPERDPGNTRLVLGAIGEWLVGPSAQLQAVGSAIGGLVTNYGELVDAGRRVFDFVRNVARGTAPSSPLNATV
SRHRRFTVASGSLEDYRTVRARYDCDVNDVVLAVIAGALGSWLMSRGVAVSPTATVRAMAPLSVYADGDLDVSGPGQAIS
QVMPFLVDLPVGEPNAVVRLSQIAHATESNPTAAHLVDARTIVTLSGFAPPTLHAMGIRVATSFPSFSKRTFNLLITNAP
GAQSQMYVAGTKLLETYSVPPLLHNQALAIGVTSYNGMLYYGINADREAMSDVDLLPALLNQALEELVEAARS
>Mature_473_residues
MVSRLSPADASFYRLENTATPMYVGSLAILRRPRAGLSYETLLHTIEQRLPQIPRYRQKVREVPVGTARPVWIDDGDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKTRPLWEMYLVEGLSKNRLALYTKSHQALINGMTALEINHVIADRTKRP
PAFPEDIWVPERDPGNTRLVLGAIGEWLVGPSAQLQAVGSAIGGLVTNYGELVDAGRRVFDFVRNVARGTAPSSPLNATV
SRHRRFTVASGSLEDYRTVRARYDCDVNDVVLAVIAGALGSWLMSRGVAVSPTATVRAMAPLSVYADGDLDVSGPGQAIS
QVMPFLVDLPVGEPNAVVRLSQIAHATESNPTAAHLVDARTIVTLSGFAPPTLHAMGIRVATSFPSFSKRTFNLLITNAP
GAQSQMYVAGTKLLETYSVPPLLHNQALAIGVTSYNGMLYYGINADREAMSDVDLLPALLNQALEELVEAARS

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity to produce wax esters [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 51421; Mature: 51421

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSRLSPADASFYRLENTATPMYVGSLAILRRPRAGLSYETLLHTIEQRLPQIPRYRQKV
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
REVPVGTARPVWIDDGDFDITYHVRRSALPSPGSDEQLHELIARLAARPLDKTRPLWEMY
HHCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
LVEGLSKNRLALYTKSHQALINGMTALEINHVIADRTKRPPAFPEDIWVPERDPGNTRLV
HHHCCCCCEEEEEECCHHHHHCCCHHEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEE
LGAIGEWLVGPSAQLQAVGSAIGGLVTNYGELVDAGRRVFDFVRNVARGTAPSSPLNATV
EEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
SRHRRFTVASGSLEDYRTVRARYDCDVNDVVLAVIAGALGSWLMSRGVAVSPTATVRAMA
HHCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCHHEEEEC
PLSVYADGDLDVSGPGQAISQVMPFLVDLPVGEPNAVVRLSQIAHATESNPTAAHLVDAR
CEEEEECCCCCCCCCHHHHHHHHHHHEECCCCCCHHHEEHHHHHHHCCCCCCHHHEECCE
TIVTLSGFAPPTLHAMGIRVATSFPSFSKRTFNLLITNAPGAQSQMYVAGTKLLETYSVP
EEEEECCCCCCCHHHHCEEEEECCCCCCCEEEEEEEECCCCCCCEEEEHHHHHHHHCCCC
PLLHNQALAIGVTSYNGMLYYGINADREAMSDVDLLPALLNQALEELVEAARS
CCCCCCEEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSRLSPADASFYRLENTATPMYVGSLAILRRPRAGLSYETLLHTIEQRLPQIPRYRQKV
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
REVPVGTARPVWIDDGDFDITYHVRRSALPSPGSDEQLHELIARLAARPLDKTRPLWEMY
HHCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
LVEGLSKNRLALYTKSHQALINGMTALEINHVIADRTKRPPAFPEDIWVPERDPGNTRLV
HHHCCCCCEEEEEECCHHHHHCCCHHEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEE
LGAIGEWLVGPSAQLQAVGSAIGGLVTNYGELVDAGRRVFDFVRNVARGTAPSSPLNATV
EEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
SRHRRFTVASGSLEDYRTVRARYDCDVNDVVLAVIAGALGSWLMSRGVAVSPTATVRAMA
HHCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCHHEEEEC
PLSVYADGDLDVSGPGQAISQVMPFLVDLPVGEPNAVVRLSQIAHATESNPTAAHLVDAR
CEEEEECCCCCCCCCHHHHHHHHHHHEECCCCCCHHHEEHHHHHHHCCCCCCHHHEECCE
TIVTLSGFAPPTLHAMGIRVATSFPSFSKRTFNLLITNAPGAQSQMYVAGTKLLETYSVP
EEEEECCCCCCCHHHHCEEEEECCCCCCCEEEEEEEECCCCCCCEEEEHHHHHHHHCCCC
PLLHNQALAIGVTSYNGMLYYGINADREAMSDVDLLPALLNQALEELVEAARS
CCCCCCEEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]