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The map label for this gene is prfB

Identifier: 41409273

GI number: 41409273

Start: 3525690

End: 3526811

Strand: Reverse

Name: prfB

Synonym: MAP3175c

Alternate gene names: 41409273

Gene position: 3526811-3525690 (Counterclockwise)

Preceding gene: 41409276

Following gene: 41409272

Centisome position: 73.02

GC content: 66.13

Gene sequence:

>1122_bases
TTGTCGGCTGTGGAACCCGACCGTCAAGCCGACATCGCCGCCCTCGACTCCACCCTTACCACGGTGGAGCGGGTGCTCGA
TGTGGACGGTCTGCGCGCCCGCATCGAGAAGCTCGAGCACGAGGCGTCCGATCCGCAGCTGTGGGATGACCAGGCCCGCG
CCCAGCGGGTGACCAGCGAGTTGTCCCACGCCCAGGGCGAGCTGCGCCGCATCGAGGAGCTGCGCGCGCGGTTGGACGAT
CTGCCGGTGCTCTACGAGCTGGCCGCGGAGGATGGCGAGGGCGCGGCCGAGGCGCTGGCGGAGGCCGACGCCGAGCTCAA
GGCGCTGCGGGCCGACATCGAGGCCACCGAGGTGCGCACCCTGCTGTCGGGCGAGTACGACGAGCGCGAGGCGCTGGTCA
CCATCCGGTCCGGCGCGGGCGGGGTGGACGCCGCCGACTGGGCCGAGATGCTGATGCGCATGTACATCCGGTGGGCCGAG
CAGCACAAGTATCCCGTCGAGGTGTTCGACACGTCCTACGCCGAAGAGGCGGGCATCAAGAGCGCGACGTTCGCCGTGCA
CGCCCCGTTCGCCTACGGCACGCTGTCGGTGGAGCAGGGCACCCACCGGCTGGTGCGCATCAGCCCGTTCGACAACCAGA
GCCGGCGTCAGACGTCGTTCGCCGAAGTCGAGGTGCTGCCGGTGGTGGAGACCACCGACCACATCGACATCCCGGAAGGC
GATGTGCGCGTTGACGTTTACCGCTCCAGCGGGCCGGGCGGCCAATCGGTGAACACCACCGACTCCGCGGTACGTTTAAC
GCACATCCCAACGGGTATCGTCGTGACTTGCCAGAACGAAAAATCGCAGTTGCAGAACAAGATCTCGGCGATGCGGGTGC
TTCAGGCAAAGTTGTTGGAGCGCAAGCGATCCGAAGAACGCGCTGAGCTGGACGCGTTAAAGGGCGAGGGTGGCAGTTCG
TGGGGCAACCAGATGCGCTCCTACGTGCTGCACCCCTATCAGATGGTCAAGGATCTGCGAACCGAGTACGAGGTTGGCAA
TCCCGCGGCCGTCCTGGACGGAGACATCGACGGCTTCCTGGAAGCGGGAATCCGTTGGCGCAACAGAAAAGATGACGAAT
AA

Upstream 100 bases:

>100_bases
CGCGGGCGCACATGATCGTGACTCGACTCTAAACGCATGACCGCTGGTCGTGACCTCAGCACTCGCCGGTGGCGGGGCCG
CTTGGCGGTCCCCGGTAACG

Downstream 100 bases:

>100_bases
CACAAGCGTTCTCGCCGGAGCCATGACGACAGCGGCGCACCGCTGGCATGACTTCTGGCGGGGGGAACTCGGTGAGTGGA
TCATCACCAGGGGTCTGCGG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSAVEPDRQADIAALDSTLTTVERVLDVDGLRARIEKLEHEASDPQLWDDQARAQRVTSELSHAQGELRRIEELRARLDD
LPVLYELAAEDGEGAAEALAEADAELKALRADIEATEVRTLLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMYIRWAE
QHKYPVEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHIDIPEG
DVRVDVYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLERKRSEERAELDALKGEGGSS
WGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGDIDGFLEAGIRWRNRKDDE

Sequences:

>Translated_373_residues
MSAVEPDRQADIAALDSTLTTVERVLDVDGLRARIEKLEHEASDPQLWDDQARAQRVTSELSHAQGELRRIEELRARLDD
LPVLYELAAEDGEGAAEALAEADAELKALRADIEATEVRTLLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMYIRWAE
QHKYPVEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHIDIPEG
DVRVDVYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLERKRSEERAELDALKGEGGSS
WGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGDIDGFLEAGIRWRNRKDDE
>Mature_372_residues
SAVEPDRQADIAALDSTLTTVERVLDVDGLRARIEKLEHEASDPQLWDDQARAQRVTSELSHAQGELRRIEELRARLDDL
PVLYELAAEDGEGAAEALAEADAELKALRADIEATEVRTLLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMYIRWAEQ
HKYPVEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHIDIPEGD
VRVDVYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLERKRSEERAELDALKGEGGSSW
GNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGDIDGFLEAGIRWRNRKDDE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=235, Percent_Identity=34.468085106383, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI34577120, Length=237, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=34.2281879194631, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=45.0292397660819, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=38.2716049382716, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17542784, Length=213, Percent_Identity=30.9859154929577, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321295, Length=309, Percent_Identity=33.9805825242718, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921226, Length=233, Percent_Identity=32.1888412017167, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41521; Mature: 41389

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVEPDRQADIAALDSTLTTVERVLDVDGLRARIEKLEHEASDPQLWDDQARAQRVTSE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LSHAQGELRRIEELRARLDDLPVLYELAAEDGEGAAEALAEADAELKALRADIEATEVRT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMYIRWAEQHKYPVEVFDTSYAEEAGIK
HHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHCCCC
SATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHIDIPEG
CCEEEEECCCEECEEEECCCCCEEEEECCCCCCHHHHCCHHEEEEEEEEECCCCCCCCCC
DVRVDVYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLE
CEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
RKRSEERAELDALKGEGGSSWGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGDIDGFL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEECCCHHHHH
EAGIRWRNRKDDE
HHCCCCCCCCCCC
>Mature Secondary Structure 
SAVEPDRQADIAALDSTLTTVERVLDVDGLRARIEKLEHEASDPQLWDDQARAQRVTSE
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LSHAQGELRRIEELRARLDDLPVLYELAAEDGEGAAEALAEADAELKALRADIEATEVRT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLSGEYDEREALVTIRSGAGGVDAADWAEMLMRMYIRWAEQHKYPVEVFDTSYAEEAGIK
HHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHCCCC
SATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVETTDHIDIPEG
CCEEEEECCCEECEEEECCCCCEEEEECCCCCCHHHHCCHHEEEEEEEEECCCCCCCCCC
DVRVDVYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKISAMRVLQAKLLE
CEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
RKRSEERAELDALKGEGGSSWGNQMRSYVLHPYQMVKDLRTEYEVGNPAAVLDGDIDGFL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEECCCHHHHH
EAGIRWRNRKDDE
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA