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The map label for this gene is fadE22

Identifier: 41409213

GI number: 41409213

Start: 3462385

End: 3464550

Strand: Reverse

Name: fadE22

Synonym: MAP3115c

Alternate gene names: 41409213

Gene position: 3464550-3462385 (Counterclockwise)

Preceding gene: 41409214

Following gene: 41409211

Centisome position: 71.73

GC content: 71.61

Gene sequence:

>2166_bases
ATGGGTATCGCACTGACCGACGACCATCGTGAACTCGCCGAGGTGGCCCGCGGGTTTCTGACGTCGCAGAAGGCGCGCTG
GGCGGCGCGGTCGCTGCTGGATGCCACCGACGAGCCGCGGCCCGGGTTCTGGCCGAACCTGGTCGAGCTGGGCTGGCTCG
GCCTGCACATCGACGAAGAGTACGGCGGCTCCGGCTTCGGGCTGCCCGAACTGGTCGTGGTCGTCGAGGAGCTCGGCCGC
GCGGTGGCGCCCGGCCCGTTCGTGCCGACCGTCATCGCGTCGGCGGTGATCGCCAAAAACGGTACGGCAGAACAGAAGTC
GCGGCTGTTGCCCGGCTTGATCGACGGAACCGTCACCGCGGGTATCGGCCTGGACAGCCGGGTGCGGCTGGGCGACGGGG
CGGCCGACGGCGAGGCCGGGATCGTGCTGGGCGCCGGGCTGGCCGGGCTGTTGCTGATCGCCGCGGGCGACGACGTGCTG
GTGCTCGAGCGGGACCGCGACGGCGTCTCGGTCGACGTGCCGGACAACTTCGACCCGACCCGCCGGTCCGGGCGGGTGCG
GCTGGACAACGTGCGGGTGTCCGACGCCGACCTGCTCGCCGGCGCGCGGCCCTCGGCGCTGGCCCGGGCCCGCACCCTGC
TGGCCGCCGAGGCAGTGGGCGGGGCCTCGGACTGTGTCGACGCGGCCGTCGCCTACGCCAAGGTGCGCCAGCAGTTCGGC
CGCACCATCGCCACCTTCCAGGCGGTCAAGCATCACTGCGCAAACATGTTGGTGGGCGCCGAGTCCGGGATCGCCGCGGT
GTGGGACGCCGCCCGCGCGGCGGGCGAGGACGAAGACCAGTTCCACCTGATCGCCGCGGTCGCCGCGGCGCTGGCCTTCC
CGGCACATGTGCGCAACGCCGAACTCAACATCCAGGTGCACGGCGGCATCGGCTTCACCTGGGAACACGACGCGCACCTG
CACCTGCGCCGGGCGGTGGTGACCGCGGCCCTGTTCGGCGGGGACGCGCCGGCCGCCGACGTCTTCGAACGCACCGCCGC
CGGCACCGTCCGGGACAACAGCCTCGACCTGCCACCGGAGGCCGAGGAGTTGCGCACCACGATCCGCGCCGACGCCGCCG
CGATCGCCGCCCTGGACAAAGACGCCCAGCTCGACAAGCTCATCGAGACCGGTTACGTGATGCCGCACTGGCCCAAACCG
TGGGGCCGGGCGGCCGACGCCGTCGAGCAATTGGTCATCGAGGAGGAGTTCCGCGCGGCCGGCATCAAGCGGCCCGACTA
CGGCATCACCGGCTGGGTGATCCTGACCCTCATCCAGCACGGAACCCCTTGGCAAATCGAAAGATTCGTGGAGAAGGCGC
TGCGCAAAGACGAAATCTGGTGCCAGCTGTTCTCCGAGCCGGACGCCGGCTCGGACGCGGCGTCGATCAAGACGCGCGCC
ACCCGGGTGGAGGGCGGCTGGAAGATCAACGGGCAGAAGGTGTGGACCAGCGGGGCGCACTACTGCGCGCGCGGGCTGGC
CACCGTGCGCACCGACCCCGACGCACCCAAGCACGCCGGCATCACCACGGTGATCGTCGACATGAAGGCGCCCGAGGTCG
AGGTGCGGCCGCTGCGGCAGATCACCGGCGGATCGGACTTCAACGAGGTCTTCTTCAACGACCTGTTCGTGCCCGACGAG
GACGTGGTGGGAACGCCCAACTCCGGGTGGACGGTCGCCCGTGCGACGCTGGGCAACGAGCGGGTCAGCATCGGCGGCAG
CGGGTCGTTCTACGAAGGCCTGGCCGACCAACTGGTGCAGCTGGCCCAGCAGCGGCCGGATCGGTTGGCGGGCGGGAAGA
TTCGCGTCGGCTCCTACCTCGCCGAGGAGACCGCGCTGCGGCTGCTGAACCTGCGGCGCGCCGCGCGCAGCGTCGAGGGC
ACCGGGCCCGGCCCGGAGGGCAACGTCACCAAGCTGAAGCTGGCCGAACACATGGTGGAGGGCGCGGCGATCATGGCGGC
GCTGCTGGGCCCCGAGGTCGCGCTGACCGACGGTCCCGGCGCCCTGTCCGGCCGGCTGATGATGGGGGCCCGCGGCATGG
CGATCGCCGGCGGCACCTCGGAGGTGACGCGCAATCAGATCGCCGAGCGGATCCTCGGCATGCCGCGCGACCCGCTGATC
AACTAG

Upstream 100 bases:

>100_bases
CGGAACCCTCCTCGCATTAAGTACACTCTTTAGGCAGCCGCCCCGCTTCCCCTCGAAGCGGGGCAGGCACCCATCCCGGT
ACGGCGAGGAGGGCTCCCGC

Downstream 100 bases:

>100_bases
CGACCGGGCCGCGTCGGCTCGCGGCTCAGGCCGGGGCGAACCGCGGGGCCGCCACCGCTGCCGCGCTCCGGCAGCAGCGT
CACCGGCATCCCGCAGGCCA

Product: FadE22

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MGIALTDDHRELAEVARGFLTSQKARWAARSLLDATDEPRPGFWPNLVELGWLGLHIDEEYGGSGFGLPELVVVVEELGR
AVAPGPFVPTVIASAVIAKNGTAEQKSRLLPGLIDGTVTAGIGLDSRVRLGDGAADGEAGIVLGAGLAGLLLIAAGDDVL
VLERDRDGVSVDVPDNFDPTRRSGRVRLDNVRVSDADLLAGARPSALARARTLLAAEAVGGASDCVDAAVAYAKVRQQFG
RTIATFQAVKHHCANMLVGAESGIAAVWDAARAAGEDEDQFHLIAAVAAALAFPAHVRNAELNIQVHGGIGFTWEHDAHL
HLRRAVVTAALFGGDAPAADVFERTAAGTVRDNSLDLPPEAEELRTTIRADAAAIAALDKDAQLDKLIETGYVMPHWPKP
WGRAADAVEQLVIEEEFRAAGIKRPDYGITGWVILTLIQHGTPWQIERFVEKALRKDEIWCQLFSEPDAGSDAASIKTRA
TRVEGGWKINGQKVWTSGAHYCARGLATVRTDPDAPKHAGITTVIVDMKAPEVEVRPLRQITGGSDFNEVFFNDLFVPDE
DVVGTPNSGWTVARATLGNERVSIGGSGSFYEGLADQLVQLAQQRPDRLAGGKIRVGSYLAEETALRLLNLRRAARSVEG
TGPGPEGNVTKLKLAEHMVEGAAIMAALLGPEVALTDGPGALSGRLMMGARGMAIAGGTSEVTRNQIAERILGMPRDPLI
N

Sequences:

>Translated_721_residues
MGIALTDDHRELAEVARGFLTSQKARWAARSLLDATDEPRPGFWPNLVELGWLGLHIDEEYGGSGFGLPELVVVVEELGR
AVAPGPFVPTVIASAVIAKNGTAEQKSRLLPGLIDGTVTAGIGLDSRVRLGDGAADGEAGIVLGAGLAGLLLIAAGDDVL
VLERDRDGVSVDVPDNFDPTRRSGRVRLDNVRVSDADLLAGARPSALARARTLLAAEAVGGASDCVDAAVAYAKVRQQFG
RTIATFQAVKHHCANMLVGAESGIAAVWDAARAAGEDEDQFHLIAAVAAALAFPAHVRNAELNIQVHGGIGFTWEHDAHL
HLRRAVVTAALFGGDAPAADVFERTAAGTVRDNSLDLPPEAEELRTTIRADAAAIAALDKDAQLDKLIETGYVMPHWPKP
WGRAADAVEQLVIEEEFRAAGIKRPDYGITGWVILTLIQHGTPWQIERFVEKALRKDEIWCQLFSEPDAGSDAASIKTRA
TRVEGGWKINGQKVWTSGAHYCARGLATVRTDPDAPKHAGITTVIVDMKAPEVEVRPLRQITGGSDFNEVFFNDLFVPDE
DVVGTPNSGWTVARATLGNERVSIGGSGSFYEGLADQLVQLAQQRPDRLAGGKIRVGSYLAEETALRLLNLRRAARSVEG
TGPGPEGNVTKLKLAEHMVEGAAIMAALLGPEVALTDGPGALSGRLMMGARGMAIAGGTSEVTRNQIAERILGMPRDPLI
N
>Mature_720_residues
GIALTDDHRELAEVARGFLTSQKARWAARSLLDATDEPRPGFWPNLVELGWLGLHIDEEYGGSGFGLPELVVVVEELGRA
VAPGPFVPTVIASAVIAKNGTAEQKSRLLPGLIDGTVTAGIGLDSRVRLGDGAADGEAGIVLGAGLAGLLLIAAGDDVLV
LERDRDGVSVDVPDNFDPTRRSGRVRLDNVRVSDADLLAGARPSALARARTLLAAEAVGGASDCVDAAVAYAKVRQQFGR
TIATFQAVKHHCANMLVGAESGIAAVWDAARAAGEDEDQFHLIAAVAAALAFPAHVRNAELNIQVHGGIGFTWEHDAHLH
LRRAVVTAALFGGDAPAADVFERTAAGTVRDNSLDLPPEAEELRTTIRADAAAIAALDKDAQLDKLIETGYVMPHWPKPW
GRAADAVEQLVIEEEFRAAGIKRPDYGITGWVILTLIQHGTPWQIERFVEKALRKDEIWCQLFSEPDAGSDAASIKTRAT
RVEGGWKINGQKVWTSGAHYCARGLATVRTDPDAPKHAGITTVIVDMKAPEVEVRPLRQITGGSDFNEVFFNDLFVPDED
VVGTPNSGWTVARATLGNERVSIGGSGSFYEGLADQLVQLAQQRPDRLAGGKIRVGSYLAEETALRLLNLRRAARSVEGT
GPGPEGNVTKLKLAEHMVEGAAIMAALLGPEVALTDGPGALSGRLMMGARGMAIAGGTSEVTRNQIAERILGMPRDPLIN

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=356, Percent_Identity=26.685393258427, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI226958412, Length=313, Percent_Identity=27.4760383386581, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI226958414, Length=313, Percent_Identity=27.4760383386581, Blast_Score=80, Evalue=1e-14,
Organism=Homo sapiens, GI4557233, Length=296, Percent_Identity=28.3783783783784, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI4501857, Length=334, Percent_Identity=25.1497005988024, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI4557231, Length=284, Percent_Identity=25.7042253521127, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI187960098, Length=378, Percent_Identity=24.6031746031746, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI87081958, Length=262, Percent_Identity=29.3893129770992, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17506239, Length=356, Percent_Identity=26.4044943820225, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17508101, Length=310, Percent_Identity=26.7741935483871, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17538396, Length=400, Percent_Identity=26.5, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17570075, Length=349, Percent_Identity=26.3610315186246, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32563615, Length=245, Percent_Identity=26.9387755102041, Blast_Score=80, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71990804, Length=279, Percent_Identity=24.3727598566308, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17534899, Length=368, Percent_Identity=26.6304347826087, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24666513, Length=352, Percent_Identity=28.6931818181818, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355753, Length=406, Percent_Identity=27.3399014778325, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24646207, Length=297, Percent_Identity=26.9360269360269, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281363737, Length=207, Percent_Identity=27.0531400966184, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24660351, Length=195, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 76480; Mature: 76349

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00482 DIHYDROOROTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIALTDDHRELAEVARGFLTSQKARWAARSLLDATDEPRPGFWPNLVELGWLGLHIDEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCEEEEEEECC
YGGSGFGLPELVVVVEELGRAVAPGPFVPTVIASAVIAKNGTAEQKSRLLPGLIDGTVTA
CCCCCCCHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHCCCEEE
GIGLDSRVRLGDGAADGEAGIVLGAGLAGLLLIAAGDDVLVLERDRDGVSVDVPDNFDPT
CCCCCCEEEECCCCCCCCCCEEEECCHHHEEEEECCCCEEEEEECCCCCEEECCCCCCCC
RRSGRVRLDNVRVSDADLLAGARPSALARARTLLAAEAVGGASDCVDAAVAYAKVRQQFG
CCCCCEEEECEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RTIATFQAVKHHCANMLVGAESGIAAVWDAARAAGEDEDQFHLIAAVAAALAFPAHVRNA
HHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
ELNIQVHGGIGFTWEHDAHLHLRRAVVTAALFGGDAPAADVFERTAAGTVRDNSLDLPPE
EEEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCC
AEELRTTIRADAAAIAALDKDAQLDKLIETGYVMPHWPKPWGRAADAVEQLVIEEEFRAA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GIKRPDYGITGWVILTLIQHGTPWQIERFVEKALRKDEIWCQLFSEPDAGSDAASIKTRA
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHH
TRVEGGWKINGQKVWTSGAHYCARGLATVRTDPDAPKHAGITTVIVDMKAPEVEVRPLRQ
EEECCCEEECCEEEECCCHHHHHHCCCEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHH
ITGGSDFNEVFFNDLFVPDEDVVGTPNSGWTVARATLGNERVSIGGSGSFYEGLADQLVQ
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
LAQQRPDRLAGGKIRVGSYLAEETALRLLNLRRAARSVEGTGPGPEGNVTKLKLAEHMVE
HHHCCCCHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
GAAIMAALLGPEVALTDGPGALSGRLMMGARGMAIAGGTSEVTRNQIAERILGMPRDPLI
HHHHHHHHHCCCEEEECCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCCCC
N
C
>Mature Secondary Structure 
GIALTDDHRELAEVARGFLTSQKARWAARSLLDATDEPRPGFWPNLVELGWLGLHIDEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCEEEEEEECC
YGGSGFGLPELVVVVEELGRAVAPGPFVPTVIASAVIAKNGTAEQKSRLLPGLIDGTVTA
CCCCCCCHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHCCCEEE
GIGLDSRVRLGDGAADGEAGIVLGAGLAGLLLIAAGDDVLVLERDRDGVSVDVPDNFDPT
CCCCCCEEEECCCCCCCCCCEEEECCHHHEEEEECCCCEEEEEECCCCCEEECCCCCCCC
RRSGRVRLDNVRVSDADLLAGARPSALARARTLLAAEAVGGASDCVDAAVAYAKVRQQFG
CCCCCEEEECEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RTIATFQAVKHHCANMLVGAESGIAAVWDAARAAGEDEDQFHLIAAVAAALAFPAHVRNA
HHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
ELNIQVHGGIGFTWEHDAHLHLRRAVVTAALFGGDAPAADVFERTAAGTVRDNSLDLPPE
EEEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCC
AEELRTTIRADAAAIAALDKDAQLDKLIETGYVMPHWPKPWGRAADAVEQLVIEEEFRAA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GIKRPDYGITGWVILTLIQHGTPWQIERFVEKALRKDEIWCQLFSEPDAGSDAASIKTRA
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHH
TRVEGGWKINGQKVWTSGAHYCARGLATVRTDPDAPKHAGITTVIVDMKAPEVEVRPLRQ
EEECCCEEECCEEEECCCHHHHHHCCCEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHH
ITGGSDFNEVFFNDLFVPDEDVVGTPNSGWTVARATLGNERVSIGGSGSFYEGLADQLVQ
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
LAQQRPDRLAGGKIRVGSYLAEETALRLLNLRRAARSVEGTGPGPEGNVTKLKLAEHMVE
HHHCCCCHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
GAAIMAALLGPEVALTDGPGALSGRLMMGARGMAIAGGTSEVTRNQIAERILGMPRDPLI
HHHHHHHHHCCCEEEECCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]