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The map label for this gene is leuB

Identifier: 41409130

GI number: 41409130

Start: 3372693

End: 3373703

Strand: Reverse

Name: leuB

Synonym: MAP3032c

Alternate gene names: 41409130

Gene position: 3373703-3372693 (Counterclockwise)

Preceding gene: 41409131

Following gene: 41409128

Centisome position: 69.85

GC content: 67.56

Gene sequence:

>1011_bases
ATGAAACTCGCGGTGATCGGCGGCGACGGGATCGGCCCGGAGGTGACGGCCGAGGCGCTCAAGGTGCTCGACGCGGTGCT
GCCCGGTGTGGACAAGACCGAATACGACCTGGGCGCAAGGCGTTACCACGCCACCGGTGAGCTGCTGCCGGACTCGGTGA
TCGACGAGTTGCGCGCGCATGACGCGATCCTGCTCGGCGCCATCGGCGACCCGTCGGTGCCCAGCGGCGTGCTGGAGCGA
GGTCTGTTGCTGCGCTTGCGTTTTGAGCTGGACCATCACATCAACTTGCGACCCGGCCGGCTGTATCCGGGAGTGAGCAG
CCCACTGGCCGGAAACCCCGACATCGATTTCGTGGTGGTGCGCGAGGGCACCGAAGGCCCCTACACGGGGACCGGCGGCG
CCATCCGCGTCGGCACGCCCAACGAAGTCGCGACCGAGGTCAGTCAGAACACCGCTTTCGGGGTGCGCCGGGTGGTCGTT
GACGCCTTCGAGCGCGCCCGGCGGCGCCGCAAGCATTTGACGCTGGTGCACAAGACGAACGTGCTGACCTTCGCCGGCAA
GTTGTGGTCGCGCATCGTGGCCGAGGTCGGCCGGGACTACCCCGATGTCGAGGTGGCATATCAGCACATCGACGCCGCCA
CCATCTTCATGGTCACCGATCCGGGCCGCTTCGACGTGATCGTCACCGACAACCTGTTCGGCGACATCATCACCGATCTG
TCGGCCGCGGTGTGCGGCGGAATTGGCTTGGCCGCCAGTGGAAATATCGACGGCACCCGGACCAATCCGTCGATGTTCGA
GCCCGTGCACGGCAGCGCGCCCGACATCGCCGGCCAAGGCATCGCCGATCCCACGGCCGCGATCATGTCGGTGGCGCTGC
TGCTCGCCCACCTCGGCGAGGACGCCGCCGCTACCCGGGTTGACCGGGCCGTCGAACGATATCTGGCAACGCGTGGGAAC
GAACGGCCCGCCACCACCGAAGTCGGCGAACGGATTGCCGCCGCGCTCTAG

Upstream 100 bases:

>100_bases
ATCGGCGCGGCGGTCGGCGCGAACAAGCTTGAGGTGGTTGATCTCTCGTGAACCGCCCCGACGACGATGCAGAGCGTAGC
GATGGGGAGGAGCGGCGCCG

Downstream 100 bases:

>100_bases
TTTCCAAGCCGGGCGGCAGCAGCTGCGTCGGCACCAGCGGCATCGCCGCCAAGGGGAACAACGCGCACAACAGCCATGCC
GGTGGATATTTCGCGGCCGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKLAVIGGDGIGPEVTAEALKVLDAVLPGVDKTEYDLGARRYHATGELLPDSVIDELRAHDAILLGAIGDPSVPSGVLER
GLLLRLRFELDHHINLRPGRLYPGVSSPLAGNPDIDFVVVREGTEGPYTGTGGAIRVGTPNEVATEVSQNTAFGVRRVVV
DAFERARRRRKHLTLVHKTNVLTFAGKLWSRIVAEVGRDYPDVEVAYQHIDAATIFMVTDPGRFDVIVTDNLFGDIITDL
SAAVCGGIGLAASGNIDGTRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLAHLGEDAAATRVDRAVERYLATRGN
ERPATTEVGERIAAAL

Sequences:

>Translated_336_residues
MKLAVIGGDGIGPEVTAEALKVLDAVLPGVDKTEYDLGARRYHATGELLPDSVIDELRAHDAILLGAIGDPSVPSGVLER
GLLLRLRFELDHHINLRPGRLYPGVSSPLAGNPDIDFVVVREGTEGPYTGTGGAIRVGTPNEVATEVSQNTAFGVRRVVV
DAFERARRRRKHLTLVHKTNVLTFAGKLWSRIVAEVGRDYPDVEVAYQHIDAATIFMVTDPGRFDVIVTDNLFGDIITDL
SAAVCGGIGLAASGNIDGTRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLAHLGEDAAATRVDRAVERYLATRGN
ERPATTEVGERIAAAL
>Mature_336_residues
MKLAVIGGDGIGPEVTAEALKVLDAVLPGVDKTEYDLGARRYHATGELLPDSVIDELRAHDAILLGAIGDPSVPSGVLER
GLLLRLRFELDHHINLRPGRLYPGVSSPLAGNPDIDFVVVREGTEGPYTGTGGAIRVGTPNEVATEVSQNTAFGVRRVVV
DAFERARRRRKHLTLVHKTNVLTFAGKLWSRIVAEVGRDYPDVEVAYQHIDAATIFMVTDPGRFDVIVTDNLFGDIITDL
SAAVCGGIGLAASGNIDGTRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLAHLGEDAAATRVDRAVERYLATRGN
ERPATTEVGERIAAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=328, Percent_Identity=33.2317073170732, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI4758582, Length=330, Percent_Identity=29.6969696969697, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178838, Length=322, Percent_Identity=29.8136645962733, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178821, Length=308, Percent_Identity=27.5974025974026, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178816, Length=308, Percent_Identity=27.5974025974026, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178819, Length=155, Percent_Identity=30.3225806451613, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788101, Length=309, Percent_Identity=44.6601941747573, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI87081683, Length=353, Percent_Identity=38.8101983002833, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787381, Length=357, Percent_Identity=31.3725490196078, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71986051, Length=311, Percent_Identity=35.048231511254, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17550882, Length=316, Percent_Identity=29.746835443038, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=336, Percent_Identity=29.1666666666667, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17505779, Length=334, Percent_Identity=27.8443113772455, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=33.5180055401662, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324709, Length=319, Percent_Identity=31.974921630094, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322097, Length=332, Percent_Identity=34.0361445783133, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=319, Percent_Identity=31.974921630094, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24643268, Length=342, Percent_Identity=33.0409356725146, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=33.0409356725146, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24661184, Length=316, Percent_Identity=32.2784810126582, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI281362242, Length=314, Percent_Identity=28.0254777070064, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648872, Length=314, Percent_Identity=28.0254777070064, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI161078633, Length=342, Percent_Identity=27.4853801169591, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24650122, Length=342, Percent_Identity=27.4853801169591, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078637, Length=342, Percent_Identity=27.4853801169591, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078635, Length=342, Percent_Identity=27.4853801169591, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=27.893175074184, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20130355, Length=314, Percent_Identity=23.2484076433121, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 35661; Mature: 35661

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVIGGDGIGPEVTAEALKVLDAVLPGVDKTEYDLGARRYHATGELLPDSVIDELRAH
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEECCCCCCCHHHHHHHHHC
DAILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPGRLYPGVSSPLAGNPDIDFVVV
CEEEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
REGTEGPYTGTGGAIRVGTPNEVATEVSQNTAFGVRRVVVDAFERARRRRKHLTLVHKTN
ECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
VLTFAGKLWSRIVAEVGRDYPDVEVAYQHIDAATIFMVTDPGRFDVIVTDNLFGDIITDL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEEEECCCCEEEEEECCHHHHHHHHH
SAAVCGGIGLAASGNIDGTRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLAHLGE
HHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DAAATRVDRAVERYLATRGNERPATTEVGERIAAAL
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLAVIGGDGIGPEVTAEALKVLDAVLPGVDKTEYDLGARRYHATGELLPDSVIDELRAH
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEECCCCCCCHHHHHHHHHC
DAILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPGRLYPGVSSPLAGNPDIDFVVV
CEEEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
REGTEGPYTGTGGAIRVGTPNEVATEVSQNTAFGVRRVVVDAFERARRRRKHLTLVHKTN
ECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
VLTFAGKLWSRIVAEVGRDYPDVEVAYQHIDAATIFMVTDPGRFDVIVTDNLFGDIITDL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEEEECCCCEEEEEECCHHHHHHHHH
SAAVCGGIGLAASGNIDGTRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLAHLGE
HHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DAAATRVDRAVERYLATRGNERPATTEVGERIAAAL
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA