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The map label for this gene is gltX

Identifier: 41409127

GI number: 41409127

Start: 3369077

End: 3370555

Strand: Reverse

Name: gltX

Synonym: MAP3029c

Alternate gene names: 41409127

Gene position: 3370555-3369077 (Counterclockwise)

Preceding gene: 41409128

Following gene: 41409124

Centisome position: 69.79

GC content: 70.18

Gene sequence:

>1479_bases
GTGACTGCACCCGCGAACGTCCGGGTCCGATTCTGCCCGTCGCCCACCGGCACCCCGCACGTCGGCATGGTCCGCACGGC
GCTGTTCAACTGGGCATACGCCCGCCACACCGGCGGCACCTTCGTGTTTCGCATCGAGGACACCGACGCGGCGCGCGACA
GCGAGGAAAGCTACCTGGCGCTGCTGGACGCGCTGCGCTGGCTCGGCCTGGACTGGGACGAGGGGCCGGAGGTGGGCGGC
CCGTACGGCCCCTACCGGCAGTCACAGCGCGGCGAGATCTACCGCGACGTGGTGGCCAAGCTGGTCGAGGCGGGCGAGGC
GTATTACGCCTTCTCCACCCCGGAGGAGGTCGAGGCCCGTCACATCGCCGCCGGCCGTAATCCCAAGCTGGGCTACGACA
ACTTCGATCGCCACCTCACCGATTCGCAGCGCGCCGCCTTCCTGGCCGAGGGCCGCCAACCGGTGGTGCGGCTGCGGATG
CCCGACGAAGACCTCGGCTGGGACGACCTGGTCCGCGGCAGCACCGTCTTCGCCGCCGGCTCGGTGCCCGACTTCGCGTT
GACCCGCGCCAACGGAGATCCCTTGTACACGTTGGTCAACCCGTGCGACGACGCGCTGATGAAGATCACCCACGTGCTGC
GCGGCGAGGACCTGCTGCCCTCGACACCGCGTCAGCTCGCGCTGTATCAGGCGCTGATCCGGATCGGAGTGGCCGAGCGC
ATCCCGCAGTTCGCCCACCTGCCAACGGTATTGGGGGAGGGCACCAAAAAGCTCTCCAAGCGCGACCCGCAGTCCAACCT
GTTCGCCCACCGCGACCGCGGCTTCATCCCCGAAGGGCTGCTCAATTACCTTGCGCTGCTGGGCTGGGCCATCGCCGACG
ACCGCGACGTGTTCAGCCTGGACGAGATGGTGGCCGCCTTCGACGTCGTCGACGTCAACTCCAATCCGGCCCGCTTCGAC
CAGAAGAAGGCCGACGCGCTCAACGCCGAGCACATCCGGATGCTGACCGCCGCGGACTTCGCCGCCAGGCTGCGCCACTA
CCTCGACGTCCACGGGCATCGCCTCGCGCTCGACGACGCGGCGTTCGCCACCGCCGCCGAGTTGGTGCAGACCCGCATCG
TGGTGCTCGGCGACGCGTGGGACCTGTTGAAGTTCCTCAACGACGACGAGTACGCGATCGACCCGAAGGCCGCCGCCAAG
GAACTGGGGCCCGACGGCGCCGCGGTGCTGGACGCGGCGCTGCCCGCGCTGGACGCCGTGACGGACTGGGCGGCGCCGCA
GATCGAGGCCGCGCTGAAGACCGCGCTCATCGACGGCCTGGGCCTCAAACCGCGCAAGGCGTTCGGACCGATCCGGGTGG
GCGCCACCGGGACGACGATCAGCCCGCCGCTGTTCGAATCGCTGCAGCTGCTGGGCCGGGACCGCAGCTTGCGGAGGTTG
CGCGCGGCGCGGGAGCGGGCCGGTCAGCACAGCGCCTGA

Upstream 100 bases:

>100_bases
CACCGGCGGGCGTGGGTCCCATCGAGGACGGCGACACCGTCTCCATCACCATCGAGGGCATCGGTACCCTCACCAATCCC
GTGGTCCGCAAAGGAAAATC

Downstream 100 bases:

>100_bases
GCGACGAGCACCACACGTGCGGATGGCCCCGGCGAGTCTTTGGTAGTCTGCACTGCGGTTCACAAAGGCCGAAGAACGAC
TGGCGGCGGCGGTCCCCGAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTAPANVRVRFCPSPTGTPHVGMVRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYLALLDALRWLGLDWDEGPEVGG
PYGPYRQSQRGEIYRDVVAKLVEAGEAYYAFSTPEEVEARHIAAGRNPKLGYDNFDRHLTDSQRAAFLAEGRQPVVRLRM
PDEDLGWDDLVRGSTVFAAGSVPDFALTRANGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALYQALIRIGVAER
IPQFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWAIADDRDVFSLDEMVAAFDVVDVNSNPARFD
QKKADALNAEHIRMLTAADFAARLRHYLDVHGHRLALDDAAFATAAELVQTRIVVLGDAWDLLKFLNDDEYAIDPKAAAK
ELGPDGAAVLDAALPALDAVTDWAAPQIEAALKTALIDGLGLKPRKAFGPIRVGATGTTISPPLFESLQLLGRDRSLRRL
RAARERAGQHSA

Sequences:

>Translated_492_residues
MTAPANVRVRFCPSPTGTPHVGMVRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYLALLDALRWLGLDWDEGPEVGG
PYGPYRQSQRGEIYRDVVAKLVEAGEAYYAFSTPEEVEARHIAAGRNPKLGYDNFDRHLTDSQRAAFLAEGRQPVVRLRM
PDEDLGWDDLVRGSTVFAAGSVPDFALTRANGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALYQALIRIGVAER
IPQFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWAIADDRDVFSLDEMVAAFDVVDVNSNPARFD
QKKADALNAEHIRMLTAADFAARLRHYLDVHGHRLALDDAAFATAAELVQTRIVVLGDAWDLLKFLNDDEYAIDPKAAAK
ELGPDGAAVLDAALPALDAVTDWAAPQIEAALKTALIDGLGLKPRKAFGPIRVGATGTTISPPLFESLQLLGRDRSLRRL
RAARERAGQHSA
>Mature_491_residues
TAPANVRVRFCPSPTGTPHVGMVRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYLALLDALRWLGLDWDEGPEVGGP
YGPYRQSQRGEIYRDVVAKLVEAGEAYYAFSTPEEVEARHIAAGRNPKLGYDNFDRHLTDSQRAAFLAEGRQPVVRLRMP
DEDLGWDDLVRGSTVFAAGSVPDFALTRANGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALYQALIRIGVAERI
PQFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWAIADDRDVFSLDEMVAAFDVVDVNSNPARFDQ
KKADALNAEHIRMLTAADFAARLRHYLDVHGHRLALDDAAFATAAELVQTRIVVLGDAWDLLKFLNDDEYAIDPKAAAKE
LGPDGAAVLDAALPALDAVTDWAAPQIEAALKTALIDGLGLKPRKAFGPIRVGATGTTISPPLFESLQLLGRDRSLRRLR
AARERAGQHSA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=339, Percent_Identity=37.1681415929204, Blast_Score=209, Evalue=6e-54,
Organism=Escherichia coli, GI1788743, Length=484, Percent_Identity=34.297520661157, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI226510925, Length=257, Percent_Identity=30.7392996108949, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71993394, Length=492, Percent_Identity=30.6910569105691, Blast_Score=209, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324540, Length=341, Percent_Identity=35.4838709677419, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24665321, Length=503, Percent_Identity=31.2127236580517, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_MYCPA (Q73VI4)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_961963.1
- ProteinModelPortal:   Q73VI4
- SMR:   Q73VI4
- EnsemblBacteria:   EBMYCT00000038150
- GeneID:   2718032
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP3029c
- NMPDR:   fig|262316.1.peg.3029
- GeneTree:   EBGT00050000016746
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54020; Mature: 53888

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPANVRVRFCPSPTGTPHVGMVRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYLA
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
LLDALRWLGLDWDEGPEVGGPYGPYRQSQRGEIYRDVVAKLVEAGEAYYAFSTPEEVEAR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHH
HIAAGRNPKLGYDNFDRHLTDSQRAAFLAEGRQPVVRLRMPDEDLGWDDLVRGSTVFAAG
HHHCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCEEEEEC
SVPDFALTRANGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALYQALIRIGVAER
CCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IPQFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWAIADDRDVFSL
CCHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
DEMVAAFDVVDVNSNPARFDQKKADALNAEHIRMLTAADFAARLRHYLDVHGHRLALDDA
HHHHHHHEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHH
AFATAAELVQTRIVVLGDAWDLLKFLNDDEYAIDPKAAAKELGPDGAAVLDAALPALDAV
HHHHHHHHHHHHEEEEECHHHHHHHHCCCCCEECCHHHHHHCCCCCHHHHHHHHHHHHHH
TDWAAPQIEAALKTALIDGLGLKPRKAFGPIRVGATGTTISPPLFESLQLLGRDRSLRRL
HHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCHHHHHH
RAARERAGQHSA
HHHHHHCCCCCC
>Mature Secondary Structure 
TAPANVRVRFCPSPTGTPHVGMVRTALFNWAYARHTGGTFVFRIEDTDAARDSEESYLA
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
LLDALRWLGLDWDEGPEVGGPYGPYRQSQRGEIYRDVVAKLVEAGEAYYAFSTPEEVEAR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHH
HIAAGRNPKLGYDNFDRHLTDSQRAAFLAEGRQPVVRLRMPDEDLGWDDLVRGSTVFAAG
HHHCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCEEEEEC
SVPDFALTRANGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALYQALIRIGVAER
CCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IPQFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWAIADDRDVFSL
CCHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
DEMVAAFDVVDVNSNPARFDQKKADALNAEHIRMLTAADFAARLRHYLDVHGHRLALDDA
HHHHHHHEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHH
AFATAAELVQTRIVVLGDAWDLLKFLNDDEYAIDPKAAAKELGPDGAAVLDAALPALDAV
HHHHHHHHHHHHEEEEECHHHHHHHHCCCCCEECCHHHHHHCCCCCHHHHHHHHHHHHHH
TDWAAPQIEAALKTALIDGLGLKPRKAFGPIRVGATGTTISPPLFESLQLLGRDRSLRRL
HHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCHHHHHH
RAARERAGQHSA
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA