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The map label for this gene is gpsI

Identifier: 41408989

GI number: 41408989

Start: 3220788

End: 3223061

Strand: Reverse

Name: gpsI

Synonym: MAP2891c

Alternate gene names: 41408989

Gene position: 3223061-3220788 (Counterclockwise)

Preceding gene: 41408990

Following gene: 41408988

Centisome position: 66.73

GC content: 68.47

Gene sequence:

>2274_bases
ATGTCTGTCGCTGAAATTGAAGAAGGCGTGTTCGAGGCGACCGCCACCATCGACAACGGGAGCTTCGGCACCCGCACCAT
CCGCTTCGAGACCGGCCGGTTGGCCCAGCAGGCCGCCGGCGCCGTCGTCGCCTACCTCGACGACGAGAACATGCTGTTGT
CGGCGACCACCGCCAGCAAGAGCCCCAAGGAGCACTTCGACTTCTTCCCGCTTACCGTCGACGTCGAGGAGCGGATGTAC
GCCGCGGGCCGCATCCCCGGCTCGTTCTTCCGCCGCGAGGGCCGCCCCTCCACCGACGCCATCCTGACCTGCCGGCTCAT
CGACCGCCCGCTGCGGCCGTCGTTCGTCGACGGCCTGCGCAACGAAATCCAGGTCGTCGTCACGATTTTGAGCCTGGACC
CCAACGACCTGTACGACGTGCTGGCGATCAACGCCGCGTCGGCCTCCACCCAGCTGGGCGGTCTGCCGTTCTCCGGCCCC
ATCGGCGGGGTGCGGGTGGCGCTGATCGACGGCACCTGGGTGGCGTTCCCGACGGTCGAGCAGCTCGAGCGCGCGGTGTT
CGACATGGTGGTGGCCGGGCGCAAGGTCGACGGCGCCGACGGGCCCGACGTCGCCATCATGATGGTCGAGGCCGAGGCCA
CCTCCAACGTCATCGAACTCATCGACGGCGGCGCCCAGGCGCCCACGGAAACCGTTGTGGCCCAAGGCCTTGAGGCCGCC
AAGCCGTTCATCGAGGTGCTGTGCACCGCGCAGCAGGAGCTCGCCGACAAGGCCGCCAGGCCCACCAGCGACTACCCGAC
CTTCCCCGACTACGGCGACGACGTCTACTACTCGGTGGCGTCGGTGGCCACCGACGAGCTGTCCAAGGCGCTGACCATCG
GCGGCAAGGCCGAACGCGACGCGCGCACCGACGAGCTCAAGGCCGAGGTGCTGGCCCGACTGGCCGAAACCTATGAGGGC
CGCGAGAAAGAGGTCAGCGCCGCGTTCCGCTCGCTGACCAAAAAGCTGGTGCGCCAGCGCATCCTGACCGACCACTTCCG
CATCGACGGCCGCGGCATCACCGACATCCGCGCGCTGTCCGCCGAGGTCGCCGTGGTTCCGCGGGCACACGGCAGCGCGC
TGTTCCAGCGCGGTGAAACCCAGATCCTGGGGGTGACCACGCTGGACATGGTCAAGATGGCCCAGCAGATCGACTCGCTG
GGGCCCGAGACCACGAAACGCTATATGCACCACTACAATTTCCCGCCGTTCTCCACTGGCGAGACCGGCCGGGTGGGCTC
GCCCAAGCGCCGCGAGATCGGCCACGGCGCGCTGGCCGAGCGCGCCCTGGTGCCGGTGCTGCCGAGCCTCGAGGACTTCC
CGTATGCCATCCGGCAGGTGTCCGAGGCGCTGGGCTCCAACGGCTCGACGTCGATGGGCTCGGTCTGCGCGTCCACGCTG
GCGCTGCTCAACGCCGGGGTGCCGCTCAAGGCGCCGGTGGCAGGCATCGCGATGGGCCTGGTGTCCGACGACATAGAAGT
TGAGGCGGGAGACGGCACAAAATCGCTTGAGCGCCGCTTCGTCACCCTGACCGACATCCTGGGCGCCGAGGACGCGTTCG
GCGACATGGACTTCAAGGTGGCCGGCACCAAGGACTTCGTCACCGCGCTGCAGCTGGACACCAAGCTCGACGGCATCCCG
TCGCAGGTGCTGGCCGGGGCGCTGAGCCAGGCCAAGGATGCCCGCCTGACCATCCTCGAGGTGATGGCCGAGGCCATCGA
CGAGCCCGACGAGATGAGCCCGTACGCGCCGCGGGTCACCACCATCCGGGTCCCGGTGGACAAGATCGGCGAGGTGATCG
GCCCCAAGGGCAAGATCATCAACGCCATCACCGAGGAGACCGGCGCCCAGATCTCCATCGAGGACGACGGCACCGTGTTC
GTCGGGGCCACCGACGGCCCGTCGGCGCAGGCCGCGATCGACCGGATCAACGCCATCGCCAACCCGCAGCTGCCCACCGT
CGGTGAGCGGTTCCTCGGAACCGTGGTCAAGACAACGGATTTCGGCGCGTTCGTGTCGCTGCTGCCCGGGCGTGACGGCC
TGGTGCACATCTCCAAACTCGGCAAGGGCAAGCGCATCGCCAAGGTCGAGGACGTGGTGAACGTCGGCGACAAGCTGCGC
GTGGAGATCGCCGACATCGACAAGCGCGGCAAGATCTCGCTGGTCCTGGTGGAGGAAGACAACTCGGCACCCGCCGACAC
CCCGGCGGCCGCCCCTGCCGATGCCACCAGCTGA

Upstream 100 bases:

>100_bases
GCTCGCAGGCCCATTCGGGTCGGTGAGTGATCCTGCAAACCCTCTCCAGTTGCGCGTGATGACGCGAAACAGCCGAATAA
GAGAGGCCGTACGGACGTCT

Downstream 100 bases:

>100_bases
CAGCAGAAGGCGAGCAGCTGACTCATCGACCGGCGCCCTGCGCGCCGGCAAGGTCGTCGAGGCGCAACCGCACCCGCACG
CCGCCCTGCGGCGCAGCACG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MSVAEIEEGVFEATATIDNGSFGTRTIRFETGRLAQQAAGAVVAYLDDENMLLSATTASKSPKEHFDFFPLTVDVEERMY
AAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQVVVTILSLDPNDLYDVLAINAASASTQLGGLPFSGP
IGGVRVALIDGTWVAFPTVEQLERAVFDMVVAGRKVDGADGPDVAIMMVEAEATSNVIELIDGGAQAPTETVVAQGLEAA
KPFIEVLCTAQQELADKAARPTSDYPTFPDYGDDVYYSVASVATDELSKALTIGGKAERDARTDELKAEVLARLAETYEG
REKEVSAAFRSLTKKLVRQRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFQRGETQILGVTTLDMVKMAQQIDSL
GPETTKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSLEDFPYAIRQVSEALGSNGSTSMGSVCASTL
ALLNAGVPLKAPVAGIAMGLVSDDIEVEAGDGTKSLERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIP
SQVLAGALSQAKDARLTILEVMAEAIDEPDEMSPYAPRVTTIRVPVDKIGEVIGPKGKIINAITEETGAQISIEDDGTVF
VGATDGPSAQAAIDRINAIANPQLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLR
VEIADIDKRGKISLVLVEEDNSAPADTPAAAPADATS

Sequences:

>Translated_757_residues
MSVAEIEEGVFEATATIDNGSFGTRTIRFETGRLAQQAAGAVVAYLDDENMLLSATTASKSPKEHFDFFPLTVDVEERMY
AAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQVVVTILSLDPNDLYDVLAINAASASTQLGGLPFSGP
IGGVRVALIDGTWVAFPTVEQLERAVFDMVVAGRKVDGADGPDVAIMMVEAEATSNVIELIDGGAQAPTETVVAQGLEAA
KPFIEVLCTAQQELADKAARPTSDYPTFPDYGDDVYYSVASVATDELSKALTIGGKAERDARTDELKAEVLARLAETYEG
REKEVSAAFRSLTKKLVRQRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFQRGETQILGVTTLDMVKMAQQIDSL
GPETTKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSLEDFPYAIRQVSEALGSNGSTSMGSVCASTL
ALLNAGVPLKAPVAGIAMGLVSDDIEVEAGDGTKSLERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIP
SQVLAGALSQAKDARLTILEVMAEAIDEPDEMSPYAPRVTTIRVPVDKIGEVIGPKGKIINAITEETGAQISIEDDGTVF
VGATDGPSAQAAIDRINAIANPQLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLR
VEIADIDKRGKISLVLVEEDNSAPADTPAAAPADATS
>Mature_756_residues
SVAEIEEGVFEATATIDNGSFGTRTIRFETGRLAQQAAGAVVAYLDDENMLLSATTASKSPKEHFDFFPLTVDVEERMYA
AGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQVVVTILSLDPNDLYDVLAINAASASTQLGGLPFSGPI
GGVRVALIDGTWVAFPTVEQLERAVFDMVVAGRKVDGADGPDVAIMMVEAEATSNVIELIDGGAQAPTETVVAQGLEAAK
PFIEVLCTAQQELADKAARPTSDYPTFPDYGDDVYYSVASVATDELSKALTIGGKAERDARTDELKAEVLARLAETYEGR
EKEVSAAFRSLTKKLVRQRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFQRGETQILGVTTLDMVKMAQQIDSLG
PETTKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSLEDFPYAIRQVSEALGSNGSTSMGSVCASTLA
LLNAGVPLKAPVAGIAMGLVSDDIEVEAGDGTKSLERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPS
QVLAGALSQAKDARLTILEVMAEAIDEPDEMSPYAPRVTTIRVPVDKIGEVIGPKGKIINAITEETGAQISIEDDGTVFV
GATDGPSAQAAIDRINAIANPQLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLRV
EIADIDKRGKISLVLVEEDNSAPADTPAAAPADATS

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=707, Percent_Identity=35.5021216407355, Blast_Score=384, Evalue=1e-106,
Organism=Escherichia coli, GI145693187, Length=720, Percent_Identity=45.2777777777778, Blast_Score=578, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI115534063, Length=749, Percent_Identity=32.4432576769025, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI281362905, Length=726, Percent_Identity=36.3636363636364, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24651641, Length=726, Percent_Identity=36.3636363636364, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24651643, Length=726, Percent_Identity=36.3636363636364, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI161079377, Length=660, Percent_Identity=36.5151515151515, Blast_Score=361, Evalue=1e-99,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_MYCPA (Q73VX0)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_961825.1
- HSSP:   P05055
- ProteinModelPortal:   Q73VX0
- SMR:   Q73VX0
- EnsemblBacteria:   EBMYCT00000040342
- GeneID:   2719781
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2891c
- NMPDR:   fig|262316.1.peg.2891
- GeneTree:   EBGT00050000017412
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591
- TIGRFAMs:   TIGR02696

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 80543; Mature: 80412

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAEIEEGVFEATATIDNGSFGTRTIRFETGRLAQQAAGAVVAYLDDENMLLSATTASK
CCHHHHHHHHHEEEEEECCCCCCCEEEEEECCHHHHHHCCEEEEEECCCCEEEEECCCCC
SPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLR
CCHHHCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEHHHCCCCCCHHHHHHH
NEIQVVVTILSLDPNDLYDVLAINAASASTQLGGLPFSGPIGGVRVALIDGTWVAFPTVE
HHEEEEEEEEECCCCHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHH
QLERAVFDMVVAGRKVDGADGPDVAIMMVEAEATSNVIELIDGGAQAPTETVVAQGLEAA
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHCHHHH
KPFIEVLCTAQQELADKAARPTSDYPTFPDYGDDVYYSVASVATDELSKALTIGGKAERD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
ARTDELKAEVLARLAETYEGREKEVSAAFRSLTKKLVRQRILTDHFRIDGRGITDIRALS
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHC
AEVAVVPRAHGSALFQRGETQILGVTTLDMVKMAQQIDSLGPETTKRYMHHYNFPPFSTG
CCEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
ETGRVGSPKRREIGHGALAERALVPVLPSLEDFPYAIRQVSEALGSNGSTSMGSVCASTL
CCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALLNAGVPLKAPVAGIAMGLVSDDIEVEAGDGTKSLERRFVTLTDILGAEDAFGDMDFKV
HHHHCCCCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
AGTKDFVTALQLDTKLDGIPSQVLAGALSQAKDARLTILEVMAEAIDEPDEMSPYAPRVT
ECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEE
TIRVPVDKIGEVIGPKGKIINAITEETGAQISIEDDGTVFVGATDGPSAQAAIDRINAIA
EEECCHHHHHHHHCCCCCEEHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHC
NPQLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLR
CCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEE
VEIADIDKRGKISLVLVEEDNSAPADTPAAAPADATS
EEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVAEIEEGVFEATATIDNGSFGTRTIRFETGRLAQQAAGAVVAYLDDENMLLSATTASK
CHHHHHHHHHEEEEEECCCCCCCEEEEEECCHHHHHHCCEEEEEECCCCEEEEECCCCC
SPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLR
CCHHHCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEHHHCCCCCCHHHHHHH
NEIQVVVTILSLDPNDLYDVLAINAASASTQLGGLPFSGPIGGVRVALIDGTWVAFPTVE
HHEEEEEEEEECCCCHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHH
QLERAVFDMVVAGRKVDGADGPDVAIMMVEAEATSNVIELIDGGAQAPTETVVAQGLEAA
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHCHHHH
KPFIEVLCTAQQELADKAARPTSDYPTFPDYGDDVYYSVASVATDELSKALTIGGKAERD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
ARTDELKAEVLARLAETYEGREKEVSAAFRSLTKKLVRQRILTDHFRIDGRGITDIRALS
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHC
AEVAVVPRAHGSALFQRGETQILGVTTLDMVKMAQQIDSLGPETTKRYMHHYNFPPFSTG
CCEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
ETGRVGSPKRREIGHGALAERALVPVLPSLEDFPYAIRQVSEALGSNGSTSMGSVCASTL
CCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALLNAGVPLKAPVAGIAMGLVSDDIEVEAGDGTKSLERRFVTLTDILGAEDAFGDMDFKV
HHHHCCCCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
AGTKDFVTALQLDTKLDGIPSQVLAGALSQAKDARLTILEVMAEAIDEPDEMSPYAPRVT
ECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEE
TIRVPVDKIGEVIGPKGKIINAITEETGAQISIEDDGTVFVGATDGPSAQAAIDRINAIA
EEECCHHHHHHHHCCCCCEEHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHC
NPQLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLR
CCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEE
VEIADIDKRGKISLVLVEEDNSAPADTPAAAPADATS
EEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA