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The map label for this gene is sigB

Identifier: 41408924

GI number: 41408924

Start: 3153258

End: 3154256

Strand: Direct

Name: sigB

Synonym: MAP2826

Alternate gene names: 41408924

Gene position: 3153258-3154256 (Clockwise)

Preceding gene: 41408923

Following gene: 41408927

Centisome position: 65.29

GC content: 66.57

Gene sequence:

>999_bases
TTGAACCCGATGACAGTACAAGCCGAACGGGAGGTCGCTATGGCGAACGCCAGCACGAGCAGATTTGACGGCGATCTGGA
TGCTCAAAGCCCCGCAGCGGACCTGGTGCGCGTGTATCTGAACGGGATCGGTAAGACGGCGTTGCTGAATGCGGCTGGCG
AAGTGGAACTCGCGAAGCGCATCGAAGCGGGGCTCTACGCCGAGCACCTGCTCGAAACGCGTAAGCGCCTCGGGGAGAAC
CGCAAACGCGATCTGGAGGCCGTGGTGCGCGACGGCCAGGCCGCGCGCCGTCATCTGCTGGAAGCGAACCTGCGCCTGGT
GGTGTCGCTGGCCAAGCGGTACACGGGTCGCGGCATGCCGTTGCTGGACCTGATCCAGGAGGGCAACCTCGGGCTGATCC
GGGCGATGGAAAAGTTCGACTACACAAAGGGATTCAAGTTCTCGACGTACGCGACGTGGTGGATCCGTCAGGCCATCACC
CGCGGCATGGCCGACCAGAGCCGCACCATCCGGCTGCCGGTGCACCTGGTCGAGCAGGTCAACAAGCTGGCGCGGATCAA
GCGGGAGATGCACCAGAACCTGGGCCGCGAGGCCACCGACGAGGAGCTGGCCGCCGAGTCGGGCATCCCGATCGACAAGA
TCAACGACCTGCTCGAGCACAGCCGCGACCCGGTGAGCCTGGACATGCCGGTGGGCTCGGAGGAGGAAGCCCCGCTGGGC
GACTTCATCGAGGACGCCGAGGCGATGTCCGCCGAGAACGCGGTGATCGCCGAGCTGCTGCACACCGACATCCGCAGCGT
GCTGGCCACCCTGGACGAGCGCGAGCACCAGGTGATCCGGCTGCGGTTCGGCCTCGACGACGGCCAGCCGCGCACGCTGG
ACCAGATCGGCAAGCTGTTCGGGCTGTCCCGGGAGCGGGTCCGCCAGATCGAGCGGGACGTGATGTCCAAACTCCGCAAC
GGGGAGCGGGCCGACCGGCTACGGTCGTACGCGAGCTGA

Upstream 100 bases:

>100_bases
CGGCGCACTTCTCAGGACATTCTCAGGTCAGCGACACATTTCTGCACGTCAACACGTGTGAGATGGGGAACCGCCGGGAA
CTCAGAAGGCTGGTCTGTCG

Downstream 100 bases:

>100_bases
TAGCTCACCCAAGCTAGGTAGCAGACGGTATGCCCGCCGCGTCAGCGGCGGGCATACTGCTTGCCGTCGACGCGTTACAC
CCATTCACGCAGCTGGCCAA

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNPMTVQAEREVAMANASTSRFDGDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKRIEAGLYAEHLLETRKRLGEN
RKRDLEAVVRDGQAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAIT
RGMADQSRTIRLPVHLVEQVNKLARIKREMHQNLGREATDEELAAESGIPIDKINDLLEHSRDPVSLDMPVGSEEEAPLG
DFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERDVMSKLRN
GERADRLRSYAS

Sequences:

>Translated_332_residues
MNPMTVQAEREVAMANASTSRFDGDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKRIEAGLYAEHLLETRKRLGEN
RKRDLEAVVRDGQAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAIT
RGMADQSRTIRLPVHLVEQVNKLARIKREMHQNLGREATDEELAAESGIPIDKINDLLEHSRDPVSLDMPVGSEEEAPLG
DFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERDVMSKLRN
GERADRLRSYAS
>Mature_332_residues
MNPMTVQAEREVAMANASTSRFDGDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKRIEAGLYAEHLLETRKRLGEN
RKRDLEAVVRDGQAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAIT
RGMADQSRTIRLPVHLVEQVNKLARIKREMHQNLGREATDEELAAESGIPIDKINDLLEHSRDPVSLDMPVGSEEEAPLG
DFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERDVMSKLRN
GERADRLRSYAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=48.3394833948339, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1789098, Length=286, Percent_Identity=39.5104895104895, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1789871, Length=234, Percent_Identity=29.0598290598291, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37390; Mature: 37390

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPMTVQAEREVAMANASTSRFDGDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKR
CCCCCCCHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHH
IEAGLYAEHLLETRKRLGENRKRDLEAVVRDGQAARRHLLEANLRLVVSLAKRYTGRGMP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQV
HHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
NKLARIKREMHQNLGREATDEELAAESGIPIDKINDLLEHSRDPVSLDMPVGSEEEAPLG
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCH
DFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHH
GLSRERVRQIERDVMSKLRNGERADRLRSYAS
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MNPMTVQAEREVAMANASTSRFDGDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKR
CCCCCCCHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHH
IEAGLYAEHLLETRKRLGENRKRDLEAVVRDGQAARRHLLEANLRLVVSLAKRYTGRGMP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQV
HHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
NKLARIKREMHQNLGREATDEELAAESGIPIDKINDLLEHSRDPVSLDMPVGSEEEAPLG
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCH
DFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHH
GLSRERVRQIERDVMSKLRNGERADRLRSYAS
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]