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The map label for this gene is pcaB

Identifier: 41408827

GI number: 41408827

Start: 3062970

End: 3064196

Strand: Reverse

Name: pcaB

Synonym: MAP2729c

Alternate gene names: 41408827

Gene position: 3064196-3062970 (Counterclockwise)

Preceding gene: 41408828

Following gene: 41408824

Centisome position: 63.44

GC content: 74.16

Gene sequence:

>1227_bases
ATGACCAACCTGTTGTGGCCGGGGGAGCACCGCGCCGGTGAGCACATGACCGACGCCGCGCTGCTGGATGCGATGGTGGC
GGTGGAATCGGCGTGGCTGGCCGCGCTGACGGGCGCCGGACTGGCACCGGCCGACTGCGCGGCGGCCGACCTGCGGAACC
TGGTGGCGGACAACGACTTCGAGACGCTAGCGGTCGGCGCCGAGGACGGCGGCAACCCCGTCATCGGGCTGGTGCGCCTG
CTGCGGGAGCGCGCCGACCCGGCCGTCGCGCCGTGGATTCACCGCGGGCTCACCAGCCAGGACGTGCTGGACACCACCCT
GATGCTGGGCGTGCGCGCGCTCGCCGACGACCTGATCGCCGAACTGCGAGAGCAGATCCGGGTGCTGTCCGCGCTGACCG
ACCGGCACCGGGCCACCCCCATGGTGGCCCGCACCCTCACCCAGCACGCCGCCCCCACCACCTTCGGCGGCAAGGCCGCC
GGCTGGCTGAACGGGGTCATCGACGCCTTCGACAATGTGATAGCCCTGGTCACCCCGGTCCAAATCGGCGGCGCGGCAGG
AACATTGGCCGCCAGCACCGAACTCGCGGCGCTGCGCACCGGTACCGCCGACCCGGCGGCGGTCTCCGTCGAGCTCGCTC
GCTGCACGGCGGCCGCGCTGGGCCTGGCGCCGCGGCCCCCCTGGCACACCGCCCGCGCACCGATCACCGCGGCCGCCGAC
GCTCTCGTCGGCTGCACCGACAGCTGGGGCCGTATCGCCGCGGACGTGGTCACCCTGGCCCGTCCGGAGATCGGCGAACT
CGGTGAGCCGGCGACCGCCACCCGCGGCGGCTCGTCGTCGATGCCGCACAAGCGAAACCCGGTGTTGTCCATCCTGATTC
GCCGGGCGGCCATCGCGGGCCCGCCGCTGGCCGCGACGCTGCACACCGCCGCCGCGCTGGCCGGCGACGAACGCCCGGAC
GGCGCCTGGCATGCCGAGTGGGACACGCTGCGCATCCTGGGCCGGCGCACCGCCGTGGCCGCATCCCAGTGCACGGAACT
GCTGGCCGGGCTGAGCGTGCACACCGCGCGGATGGCCGAAAACCTCGCAGCCGCAGACGTTTTGGGTGAACAACGGGCGA
TCGCCGAGCTGGCCGGCGGCGCACCGTCACCGACCTACTTCGGCGCCGTCGACCGGGTGATCGACGAGACGCTGCGCCGC
GCCGCGCGGGTGCGTCCCGAGCGGTGA

Upstream 100 bases:

>100_bases
TGCTGTGCGTCGCCGAAAACGGCGGCCGCGCTTACCGATTCGATATCCGCCTGCAGGGACCTGGCGAGACGGTGTTCCTG
GCCTACCGGACGGACGAGCG

Downstream 100 bases:

>100_bases
GCGATCCGGCGTCTCAGTCGGCCACCGCGATCTCGATGGACAGGCACTCGCCCAGGGCGTGCGCGGCCGCCCAGTCGCCG
CCGCGCACCGCGCGTTCGAG

Product: PcaB

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTNLLWPGEHRAGEHMTDAALLDAMVAVESAWLAALTGAGLAPADCAAADLRNLVADNDFETLAVGAEDGGNPVIGLVRL
LRERADPAVAPWIHRGLTSQDVLDTTLMLGVRALADDLIAELREQIRVLSALTDRHRATPMVARTLTQHAAPTTFGGKAA
GWLNGVIDAFDNVIALVTPVQIGGAAGTLAASTELAALRTGTADPAAVSVELARCTAAALGLAPRPPWHTARAPITAAAD
ALVGCTDSWGRIAADVVTLARPEIGELGEPATATRGGSSSMPHKRNPVLSILIRRAAIAGPPLAATLHTAAALAGDERPD
GAWHAEWDTLRILGRRTAVAASQCTELLAGLSVHTARMAENLAAADVLGEQRAIAELAGGAPSPTYFGAVDRVIDETLRR
AARVRPER

Sequences:

>Translated_408_residues
MTNLLWPGEHRAGEHMTDAALLDAMVAVESAWLAALTGAGLAPADCAAADLRNLVADNDFETLAVGAEDGGNPVIGLVRL
LRERADPAVAPWIHRGLTSQDVLDTTLMLGVRALADDLIAELREQIRVLSALTDRHRATPMVARTLTQHAAPTTFGGKAA
GWLNGVIDAFDNVIALVTPVQIGGAAGTLAASTELAALRTGTADPAAVSVELARCTAAALGLAPRPPWHTARAPITAAAD
ALVGCTDSWGRIAADVVTLARPEIGELGEPATATRGGSSSMPHKRNPVLSILIRRAAIAGPPLAATLHTAAALAGDERPD
GAWHAEWDTLRILGRRTAVAASQCTELLAGLSVHTARMAENLAAADVLGEQRAIAELAGGAPSPTYFGAVDRVIDETLRR
AARVRPER
>Mature_407_residues
TNLLWPGEHRAGEHMTDAALLDAMVAVESAWLAALTGAGLAPADCAAADLRNLVADNDFETLAVGAEDGGNPVIGLVRLL
RERADPAVAPWIHRGLTSQDVLDTTLMLGVRALADDLIAELREQIRVLSALTDRHRATPMVARTLTQHAAPTTFGGKAAG
WLNGVIDAFDNVIALVTPVQIGGAAGTLAASTELAALRTGTADPAAVSVELARCTAAALGLAPRPPWHTARAPITAAADA
LVGCTDSWGRIAADVVTLARPEIGELGEPATATRGGSSSMPHKRNPVLSILIRRAAIAGPPLAATLHTAAALAGDERPDG
AWHAEWDTLRILGRRTAVAASQCTELLAGLSVHTARMAENLAAADVLGEQRAIAELAGGAPSPTYFGAVDRVIDETLRRA
ARVRPER

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Drosophila melanogaster, GI24647570, Length=264, Percent_Identity=26.8939393939394, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 42331; Mature: 42199

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLLWPGEHRAGEHMTDAALLDAMVAVESAWLAALTGAGLAPADCAAADLRNLVADNDF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
ETLAVGAEDGGNPVIGLVRLLRERADPAVAPWIHRGLTSQDVLDTTLMLGVRALADDLIA
CEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ELREQIRVLSALTDRHRATPMVARTLTQHAAPTTFGGKAAGWLNGVIDAFDNVIALVTPV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QIGGAAGTLAASTELAALRTGTADPAAVSVELARCTAAALGLAPRPPWHTARAPITAAAD
EECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
ALVGCTDSWGRIAADVVTLARPEIGELGEPATATRGGSSSMPHKRNPVLSILIRRAAIAG
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PPLAATLHTAAALAGDERPDGAWHAEWDTLRILGRRTAVAASQCTELLAGLSVHTARMAE
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLAAADVLGEQRAIAELAGGAPSPTYFGAVDRVIDETLRRAARVRPER
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNLLWPGEHRAGEHMTDAALLDAMVAVESAWLAALTGAGLAPADCAAADLRNLVADNDF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
ETLAVGAEDGGNPVIGLVRLLRERADPAVAPWIHRGLTSQDVLDTTLMLGVRALADDLIA
CEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ELREQIRVLSALTDRHRATPMVARTLTQHAAPTTFGGKAAGWLNGVIDAFDNVIALVTPV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QIGGAAGTLAASTELAALRTGTADPAAVSVELARCTAAALGLAPRPPWHTARAPITAAAD
EECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
ALVGCTDSWGRIAADVVTLARPEIGELGEPATATRGGSSSMPHKRNPVLSILIRRAAIAG
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PPLAATLHTAAALAGDERPDGAWHAEWDTLRILGRRTAVAASQCTELLAGLSVHTARMAE
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLAAADVLGEQRAIAELAGGAPSPTYFGAVDRVIDETLRRAARVRPER
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9582432; 12597275 [H]