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The map label for this gene is ybjS [C]

Identifier: 41408786

GI number: 41408786

Start: 3022665

End: 3023765

Strand: Direct

Name: ybjS [C]

Synonym: MAP2688

Alternate gene names: 41408786

Gene position: 3022665-3023765 (Clockwise)

Preceding gene: 41408785

Following gene: 41408790

Centisome position: 62.58

GC content: 66.67

Gene sequence:

>1101_bases
ATGATCGGCCCCATGGGTGATCCATCGCTGACGGCCGAACTGGGTCGCGTTCTGGTCACCGGGGGCTCCGGGTTCGTCGG
CGCCAACCTGGTGACCACCCTGCTCGAGCGCGGATATCAGGTTCGCTCCTTCGACCGCGCCCCCTCGCCGCTGCCGGCGC
ACCCACACCTGGAGGTGCTGCAGGGCGACATCACCGACGCCGGTGTGTGCGCCGCCGTGGTCGAGGGCATCGACACGATC
TTTCACACCGCGGCGATCATCGACCTGATGGGCGGCGCCTCGGTCACCGAGGAGTACCGGCAGCGCAGCTTCGCGGTGAA
CGTCGGCGGCACCGAGAACCTGGTCCGTGCCGGGCAGGCGGCCGGCGTGCAGCGCTTCGTCTACACCTCGTCGAACAGCG
TCGTCATGGGCGGCCAGAACATCGTCGGCGGCGACGAGACCTTGCCCTACACCGACCGGTTCAACGACCTCTACACCGAG
ACCAAGGTGCTCGCCGAGCGATTCGTCTTGGGCCAGAACGGCGTTGACGGCATGCTGACGTGTGCGATCCGGCCCAGTGG
CATCTGGGGCCGGGGCGACCAAACCATGTTCCGCAAGCTGTTCGAAAGCGTGATCGCCGGCCACGTCAAGGTGCTGATCG
GCCGCAAATCCGCCCGGTTGGACAACTCCTACGTGCACAACCTGATTCACGGTTTCATCCTGGCGGACCAGCACCTGACG
CCCGGCGGCACCGCGCCCGGTCAGGCGTACTTCATCAACGACGCCGAGCCGATCAACATGTTCGAGTTCGCCCGGCCGGT
GGTCGAGGCCTGCGGCGTGAACTGGCCCCGGGTCCGGGTCAACGGCCCCATCGTGCGCGCGGCGATGACGGGTTGGCAGC
GGCTGCATTTCAGGTTCGGGATCCCGGCGCCGCTGCTCGAGCCGCTCGCGGTCGAGCGGCTGTATCTGGACAACTTCTTC
TCCATCGCCAAGGCCTCGCGCGACCTCGGCTATCAGCCGCTGTTCACCACCGAGCAGGCGATGTCGGAATGCCTGCCGTA
CTACGTCGGCATGTTCGAACAGATGAAGCGACAAGCCTTGGCGGGCAAGGCATCCGCCTAG

Upstream 100 bases:

>100_bases
GAAGACGCCCTCGCCGCGGGCGAGGCTGACGACGACTGAATTCGACCCTATTGGAGAGCTTCGTTGCCACACTTTTTCGA
AACTGGAACACGTTTCAGTT

Downstream 100 bases:

>100_bases
CGGAAGCTGACCATCTCACTGCCCCAGGCGGCACGGGCCTTGGCGTCCACGTCGTGGGCCAGCACGTCGTTCTTGCCGAT
GATCAGGCAGGCGCGGTCCT

Product: hypothetical protein

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MIGPMGDPSLTAELGRVLVTGGSGFVGANLVTTLLERGYQVRSFDRAPSPLPAHPHLEVLQGDITDAGVCAAVVEGIDTI
FHTAAIIDLMGGASVTEEYRQRSFAVNVGGTENLVRAGQAAGVQRFVYTSSNSVVMGGQNIVGGDETLPYTDRFNDLYTE
TKVLAERFVLGQNGVDGMLTCAIRPSGIWGRGDQTMFRKLFESVIAGHVKVLIGRKSARLDNSYVHNLIHGFILADQHLT
PGGTAPGQAYFINDAEPINMFEFARPVVEACGVNWPRVRVNGPIVRAAMTGWQRLHFRFGIPAPLLEPLAVERLYLDNFF
SIAKASRDLGYQPLFTTEQAMSECLPYYVGMFEQMKRQALAGKASA

Sequences:

>Translated_366_residues
MIGPMGDPSLTAELGRVLVTGGSGFVGANLVTTLLERGYQVRSFDRAPSPLPAHPHLEVLQGDITDAGVCAAVVEGIDTI
FHTAAIIDLMGGASVTEEYRQRSFAVNVGGTENLVRAGQAAGVQRFVYTSSNSVVMGGQNIVGGDETLPYTDRFNDLYTE
TKVLAERFVLGQNGVDGMLTCAIRPSGIWGRGDQTMFRKLFESVIAGHVKVLIGRKSARLDNSYVHNLIHGFILADQHLT
PGGTAPGQAYFINDAEPINMFEFARPVVEACGVNWPRVRVNGPIVRAAMTGWQRLHFRFGIPAPLLEPLAVERLYLDNFF
SIAKASRDLGYQPLFTTEQAMSECLPYYVGMFEQMKRQALAGKASA
>Mature_366_residues
MIGPMGDPSLTAELGRVLVTGGSGFVGANLVTTLLERGYQVRSFDRAPSPLPAHPHLEVLQGDITDAGVCAAVVEGIDTI
FHTAAIIDLMGGASVTEEYRQRSFAVNVGGTENLVRAGQAAGVQRFVYTSSNSVVMGGQNIVGGDETLPYTDRFNDLYTE
TKVLAERFVLGQNGVDGMLTCAIRPSGIWGRGDQTMFRKLFESVIAGHVKVLIGRKSARLDNSYVHNLIHGFILADQHLT
PGGTAPGQAYFINDAEPINMFEFARPVVEACGVNWPRVRVNGPIVRAAMTGWQRLHFRFGIPAPLLEPLAVERLYLDNFF
SIAKASRDLGYQPLFTTEQAMSECLPYYVGMFEQMKRQALAGKASA

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=348, Percent_Identity=32.7586206896552, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI8393516, Length=348, Percent_Identity=32.7586206896552, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI310132178, Length=338, Percent_Identity=33.7278106508876, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI310113012, Length=338, Percent_Identity=33.7278106508876, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI239745448, Length=338, Percent_Identity=33.7278106508876, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI116268111, Length=332, Percent_Identity=35.2409638554217, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4504507, Length=381, Percent_Identity=29.9212598425197, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI260763931, Length=376, Percent_Identity=29.5212765957447, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4504509, Length=376, Percent_Identity=29.5212765957447, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI19923621, Length=351, Percent_Identity=32.7635327635328, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI218563686, Length=188, Percent_Identity=36.7021276595745, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI218563684, Length=188, Percent_Identity=36.7021276595745, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI87081792, Length=336, Percent_Identity=24.702380952381, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786974, Length=258, Percent_Identity=26.7441860465116, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17570557, Length=358, Percent_Identity=29.3296089385475, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17509709, Length=372, Percent_Identity=28.2258064516129, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI193211272, Length=334, Percent_Identity=26.9461077844311, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321437, Length=344, Percent_Identity=27.6162790697674, Blast_Score=112, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 39729; Mature: 39729

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGPMGDPSLTAELGRVLVTGGSGFVGANLVTTLLERGYQVRSFDRAPSPLPAHPHLEVL
CCCCCCCCHHHHHHCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHH
QGDITDAGVCAAVVEGIDTIFHTAAIIDLMGGASVTEEYRQRSFAVNVGGTENLVRAGQA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCHHHHHHHHHH
AGVQRFVYTSSNSVVMGGQNIVGGDETLPYTDRFNDLYTETKVLAERFVLGQNGVDGMLT
CCCEEEEEECCCCEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
CAIRPSGIWGRGDQTMFRKLFESVIAGHVKVLIGRKSARLDNSYVHNLIHGFILADQHLT
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCHHHCCHHHHHHHHHHHHHCCCCCC
PGGTAPGQAYFINDAEPINMFEFARPVVEACGVNWPRVRVNGPIVRAAMTGWQRLHFRFG
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHEEEECC
IPAPLLEPLAVERLYLDNFFSIAKASRDLGYQPLFTTEQAMSECLPYYVGMFEQMKRQAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGKASA
CCCCCC
>Mature Secondary Structure
MIGPMGDPSLTAELGRVLVTGGSGFVGANLVTTLLERGYQVRSFDRAPSPLPAHPHLEVL
CCCCCCCCHHHHHHCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHH
QGDITDAGVCAAVVEGIDTIFHTAAIIDLMGGASVTEEYRQRSFAVNVGGTENLVRAGQA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCHHHHHHHHHH
AGVQRFVYTSSNSVVMGGQNIVGGDETLPYTDRFNDLYTETKVLAERFVLGQNGVDGMLT
CCCEEEEEECCCCEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
CAIRPSGIWGRGDQTMFRKLFESVIAGHVKVLIGRKSARLDNSYVHNLIHGFILADQHLT
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCHHHCCHHHHHHHHHHHHHCCCCCC
PGGTAPGQAYFINDAEPINMFEFARPVVEACGVNWPRVRVNGPIVRAAMTGWQRLHFRFG
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHEEEECC
IPAPLLEPLAVERLYLDNFFSIAKASRDLGYQPLFTTEQAMSECLPYYVGMFEQMKRQAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGKASA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]