Click here to switch to the map view.

The map label for this gene is echA1_1

Identifier: 41408743

GI number: 41408743

Start: 2978520

End: 2979311

Strand: Reverse

Name: echA1_1

Synonym: MAP2645c

Alternate gene names: 41408743

Gene position: 2979311-2978520 (Counterclockwise)

Preceding gene: 41408744

Following gene: 41408739

Centisome position: 61.69

GC content: 72.47

Gene sequence:

>792_bases
GTGACCGACGCGCCGGGGGCCCTGGCCGAACGCCGGGGCAACGTGATGGTGATAACCATCAACCGGCCGGAAGCCCGCAA
CGCGATCAACGCCGCGGTCAGCATCGGCGTCGGGGATGCGCTCGAAGAGGCGCAACACGACCCCGAGGTGCGGGCGGTGG
TGCTCACCGGCGCGGGCGACAAGTCGTTCTGCGCCGGGGCCGACCTCAAGGCCATCGCGCGGCGGGAGAACCTGTATCAC
CCCGACCATCCCGAATGGGGCTTCGCCGGCTACGTGCGTCACTTCATCGACAAGCCCACCATCGCGGCGGTCAACGGCAC
CGCCCTGGGCGGGGGCACCGAGCTGGCGCTGGCCAGCGACCTGGTGGTGGCCGACGAGCGGGCCCAGTTCGGGCTGCCGG
AGGTCAAGCGCGGCCTGATCGCCGCCGCCGGTGGGGTGTTCCGGATCGCCGAGCAGTTACCCCGCAAGGTGGCGATGCGG
CTGCTGCTGACCGGCGAGCCGCTGTCCGCGGCCGCTGCCCGCGACTGGGGCCTGATCAACGAGGTGGTCGAGGCCGGCTC
GGTGCTGGACGCGGCGCTGGCGCTGGCCTCGGCGATCACCGTGAACGCCCCGCTGTCGGTGCAGGCCAGCAAGCGGATCG
CCTACGGCGTCGACGACGGCGTGGTGGTCGGCGACGAACCCGGCTGGGACCGCACCATGCGCGAGATGCGGGCCCTGCTC
AAATCGGAGGACGCCAAGGAGGGCCCGCGGGCGTTCGCCGAGAAGCGGGAGCCGGTCTGGCAGGCGCGCTGA

Upstream 100 bases:

>100_bases
CCACGCTGCTGTATCACATGCGGGACAAGGGAATTCGTTACGGCCTGCAGACCATGTGCGAGGGCGGCGGGCAGGCCAAC
GCCACCATCGTGGAACTGTT

Downstream 100 bases:

>100_bases
ACCCACGCCCTAGCGGGCCAGGATGTTCACCCCGGCGCGGAACGCGCCGGGCAACAGCGCGCCGGCCGGCACGATGGCGC
GGCGCACCGCCCGCGGTGTG

Product: EchA1_1

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTDAPGALAERRGNVMVITINRPEARNAINAAVSIGVGDALEEAQHDPEVRAVVLTGAGDKSFCAGADLKAIARRENLYH
PDHPEWGFAGYVRHFIDKPTIAAVNGTALGGGTELALASDLVVADERAQFGLPEVKRGLIAAAGGVFRIAEQLPRKVAMR
LLLTGEPLSAAAARDWGLINEVVEAGSVLDAALALASAITVNAPLSVQASKRIAYGVDDGVVVGDEPGWDRTMREMRALL
KSEDAKEGPRAFAEKREPVWQAR

Sequences:

>Translated_263_residues
MTDAPGALAERRGNVMVITINRPEARNAINAAVSIGVGDALEEAQHDPEVRAVVLTGAGDKSFCAGADLKAIARRENLYH
PDHPEWGFAGYVRHFIDKPTIAAVNGTALGGGTELALASDLVVADERAQFGLPEVKRGLIAAAGGVFRIAEQLPRKVAMR
LLLTGEPLSAAAARDWGLINEVVEAGSVLDAALALASAITVNAPLSVQASKRIAYGVDDGVVVGDEPGWDRTMREMRALL
KSEDAKEGPRAFAEKREPVWQAR
>Mature_262_residues
TDAPGALAERRGNVMVITINRPEARNAINAAVSIGVGDALEEAQHDPEVRAVVLTGAGDKSFCAGADLKAIARRENLYHP
DHPEWGFAGYVRHFIDKPTIAAVNGTALGGGTELALASDLVVADERAQFGLPEVKRGLIAAAGGVFRIAEQLPRKVAMRL
LLTGEPLSAAAARDWGLINEVVEAGSVLDAALALASAITVNAPLSVQASKRIAYGVDDGVVVGDEPGWDRTMREMRALLK
SEDAKEGPRAFAEKREPVWQAR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=36.078431372549, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=29.6, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI37594471, Length=186, Percent_Identity=30.1075268817204, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=186, Percent_Identity=30.1075268817204, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=31.2252964426877, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=29.2134831460674, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4502327, Length=269, Percent_Identity=31.5985130111524, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=31.413612565445, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=42.578125, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=35.9375, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1788597, Length=247, Percent_Identity=29.5546558704453, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=28.2868525896414, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=31.6602316602317, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790281, Length=199, Percent_Identity=28.1407035175879, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=36.8627450980392, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=36.9047619047619, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=35.1485148514851, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=209, Percent_Identity=32.0574162679426, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144160, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=262, Percent_Identity=32.0610687022901, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=28.8770053475936, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=184, Percent_Identity=29.8913043478261, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=184, Percent_Identity=29.8913043478261, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=184, Percent_Identity=29.8913043478261, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=28.8288288288288, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=208, Percent_Identity=27.4038461538462, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=224, Percent_Identity=26.7857142857143, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=213, Percent_Identity=29.1079812206573, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=35.8870967741936, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=35.8870967741936, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24653139, Length=273, Percent_Identity=34.7985347985348, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21357171, Length=199, Percent_Identity=34.1708542713568, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650670, Length=195, Percent_Identity=32.8205128205128, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571730, Length=197, Percent_Identity=28.4263959390863, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571729, Length=197, Percent_Identity=28.4263959390863, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24654903, Length=236, Percent_Identity=26.6949152542373, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=24.2424242424242, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921000, Length=177, Percent_Identity=32.7683615819209, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583077, Length=177, Percent_Identity=32.7683615819209, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583079, Length=177, Percent_Identity=32.7683615819209, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27846; Mature: 27715

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAPGALAERRGNVMVITINRPEARNAINAAVSIGVGDALEEAQHDPEVRAVVLTGAGD
CCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEECCCC
KSFCAGADLKAIARRENLYHPDHPEWGFAGYVRHFIDKPTIAAVNGTALGGGTELALASD
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHC
LVVADERAQFGLPEVKRGLIAAAGGVFRIAEQLPRKVAMRLLLTGEPLSAAAARDWGLIN
EEEECCHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EVVEAGSVLDAALALASAITVNAPLSVQASKRIAYGVDDGVVVGDEPGWDRTMREMRALL
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCEEECCCCCHHHHHHHHHHHH
KSEDAKEGPRAFAEKREPVWQAR
HCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDAPGALAERRGNVMVITINRPEARNAINAAVSIGVGDALEEAQHDPEVRAVVLTGAGD
CCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEECCCC
KSFCAGADLKAIARRENLYHPDHPEWGFAGYVRHFIDKPTIAAVNGTALGGGTELALASD
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHC
LVVADERAQFGLPEVKRGLIAAAGGVFRIAEQLPRKVAMRLLLTGEPLSAAAARDWGLIN
EEEECCHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EVVEAGSVLDAALALASAITVNAPLSVQASKRIAYGVDDGVVVGDEPGWDRTMREMRALL
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCEEECCCCCHHHHHHHHHHHH
KSEDAKEGPRAFAEKREPVWQAR
HCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA