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The map label for this gene is fadD19_3

Identifier: 41408486

GI number: 41408486

Start: 2683765

End: 2685402

Strand: Reverse

Name: fadD19_3

Synonym: MAP2388c

Alternate gene names: 41408486

Gene position: 2685402-2683765 (Counterclockwise)

Preceding gene: 41408487

Following gene: 41408485

Centisome position: 55.6

GC content: 68.01

Gene sequence:

>1638_bases
GTGAGCGAATGGACCATAGGGGCGGTTCTCGACGAAATCGCCGACGTCATCCCCGACCGCACGATGACGGTGTGCGGCGA
CCGCCGCAGCACGTTCGCCGAGTCGGCGGACCGCACCCGCCGGTTGGCGAACTTCCTGTCCGGCAACGGATTGGGCGTGC
ACCGGGAACGCGCGGTCCTGCAGAACTGGGAGTGCGGGCAGGACCGGGTCGCCCTGGTCATGCACAACGATCTGTACCCG
GACATGGTCGTCGGCTGCCTCAAGGCGCGCACCGTGCCGGTGAACGTCAACTACCACTACACGCCGCGTGAGGTCGGTGA
GCTCTTCGACTACCTGAGACCGCGGGCGGTCATCTACCACCGCGGGCTGGGGCCGAAGTTCGCCGACGTCCTCGGCCGCG
GCGACGTCGACCTGCTCATCGCGGTCGACGACGGCAGCGAGGCCGCGCAACTGCCGGGCGCGGTGTCGCTGGATGATGCT
CTGGCCCAAGGCGATACCGGTCACCCGGCGCCGGGATCGCCGGACGATCTGCTGATGATCTGCACCGGCGGGACGACGGG
CCGGCCCAAGGGGGTGCTGTGGCGCCAGTCCGACATCTACGTGTCGTCGATGGTGGGGGCGGATCACGCCTGCGCTCAGG
AGATCCGCGACAAGGTCAGCGGCGCCGCCGGGGCGCCGTGGTTCGCCGTCTCGCCGCTGATGCACGCGGCCGGCATGTGG
ACGGCGTTCGCGGCGATCATGGCGGGGACCACCGTGGTGCTCTACGACACCGGCAAGAAGCTCGACCCGCGATCGGTCTG
GGAGACCGCCCAGCGTGAGCGGGTCGGGATGATGACCATGGTCGGCGACGCCTATGCGGCACCGCTGGTCGCCGAATTGC
AGCGCGGCTCCTATGATTTGTCGTCGCTGTACGCCATCGGCACCGGGGGAGCGGCAACCAACCCCAAATACCAGCAGGCC
CTGCTGGAGCTGCTTCCCCACATCACTCTCATCAACGGTTACGGGTCGTCGGAGACCGGCAACATGGGATTCGGCCACAG
CCGAACCGGCACCCGAACCGACACCTTCACACTGCGCGAGGGTGGGCTGGTGCTCGCCGAGGACTACAGCCGCTTCCTTT
CCCCCGGCGAGCCGCAGCTGGGCTGGGTCGCCCGCGAGGGACGAATCCCGTTGGGGTACTTCGACGATCCCGACGCCACC
CGCAAGACCTTTCCGGTGATCGACGGCAAACAAGTGGTGATCTCCGGAGACCGCGCCGCCCTGGAACCCGACGGAACACT
GCGGTTGTTCGGGCGGGACTCGCTGGTGGTCAACACCGGTGGGGAGAAGGTCTTCGTCGAGGAGGTCGAGGAGGTGCTGC
GCGCCCACCCGGCCGTCGCCGACGCGCTGGTGGTCGGGCGGCCCAGCGGGCGTTGGGGCGAGGAAATCGTCGCGCTGGTG
GAACTTCGTGCCGGAACCGGTGCCGCCGCCGACGAGTTGCACGCGCACTGCACGTCGCGGCTGGCGCGGTTCAAGGCGCC
CAAGGAATTTCTGTTCGTCGCTGCGGTTCAGCGCTTGGGCAACGGTAAGGCGGATTACCGTTGGGCCAAACGTCACGCTG
TCACCGAAGCTAGCGAGAAGGCGCCGATGTCGACATGA

Upstream 100 bases:

>100_bases
AGCGAGTCCGACATTCGAAAGATCATGCGGGACAACGCCCTCGATCTGCTCGGCGTGCAAGTCGGCTCGGCCGCCTGAGT
ATCCGGGACCGACGACGCCC

Downstream 100 bases:

>100_bases
GAGCAATCGATTGTCTGGTCAACGTGCACTTCGGCGAGACCGAGAAGCAGCCGACGTGGATGCTCAAGGTGCGCGACGAC
TACTTCAAGGGACCGGCGTC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSEWTIGAVLDEIADVIPDRTMTVCGDRRSTFAESADRTRRLANFLSGNGLGVHRERAVLQNWECGQDRVALVMHNDLYP
DMVVGCLKARTVPVNVNYHYTPREVGELFDYLRPRAVIYHRGLGPKFADVLGRGDVDLLIAVDDGSEAAQLPGAVSLDDA
LAQGDTGHPAPGSPDDLLMICTGGTTGRPKGVLWRQSDIYVSSMVGADHACAQEIRDKVSGAAGAPWFAVSPLMHAAGMW
TAFAAIMAGTTVVLYDTGKKLDPRSVWETAQRERVGMMTMVGDAYAAPLVAELQRGSYDLSSLYAIGTGGAATNPKYQQA
LLELLPHITLINGYGSSETGNMGFGHSRTGTRTDTFTLREGGLVLAEDYSRFLSPGEPQLGWVAREGRIPLGYFDDPDAT
RKTFPVIDGKQVVISGDRAALEPDGTLRLFGRDSLVVNTGGEKVFVEEVEEVLRAHPAVADALVVGRPSGRWGEEIVALV
ELRAGTGAAADELHAHCTSRLARFKAPKEFLFVAAVQRLGNGKADYRWAKRHAVTEASEKAPMST

Sequences:

>Translated_545_residues
MSEWTIGAVLDEIADVIPDRTMTVCGDRRSTFAESADRTRRLANFLSGNGLGVHRERAVLQNWECGQDRVALVMHNDLYP
DMVVGCLKARTVPVNVNYHYTPREVGELFDYLRPRAVIYHRGLGPKFADVLGRGDVDLLIAVDDGSEAAQLPGAVSLDDA
LAQGDTGHPAPGSPDDLLMICTGGTTGRPKGVLWRQSDIYVSSMVGADHACAQEIRDKVSGAAGAPWFAVSPLMHAAGMW
TAFAAIMAGTTVVLYDTGKKLDPRSVWETAQRERVGMMTMVGDAYAAPLVAELQRGSYDLSSLYAIGTGGAATNPKYQQA
LLELLPHITLINGYGSSETGNMGFGHSRTGTRTDTFTLREGGLVLAEDYSRFLSPGEPQLGWVAREGRIPLGYFDDPDAT
RKTFPVIDGKQVVISGDRAALEPDGTLRLFGRDSLVVNTGGEKVFVEEVEEVLRAHPAVADALVVGRPSGRWGEEIVALV
ELRAGTGAAADELHAHCTSRLARFKAPKEFLFVAAVQRLGNGKADYRWAKRHAVTEASEKAPMST
>Mature_544_residues
SEWTIGAVLDEIADVIPDRTMTVCGDRRSTFAESADRTRRLANFLSGNGLGVHRERAVLQNWECGQDRVALVMHNDLYPD
MVVGCLKARTVPVNVNYHYTPREVGELFDYLRPRAVIYHRGLGPKFADVLGRGDVDLLIAVDDGSEAAQLPGAVSLDDAL
AQGDTGHPAPGSPDDLLMICTGGTTGRPKGVLWRQSDIYVSSMVGADHACAQEIRDKVSGAAGAPWFAVSPLMHAAGMWT
AFAAIMAGTTVVLYDTGKKLDPRSVWETAQRERVGMMTMVGDAYAAPLVAELQRGSYDLSSLYAIGTGGAATNPKYQQAL
LELLPHITLINGYGSSETGNMGFGHSRTGTRTDTFTLREGGLVLAEDYSRFLSPGEPQLGWVAREGRIPLGYFDDPDATR
KTFPVIDGKQVVISGDRAALEPDGTLRLFGRDSLVVNTGGEKVFVEEVEEVLRAHPAVADALVVGRPSGRWGEEIVALVE
LRAGTGAAADELHAHCTSRLARFKAPKEFLFVAAVQRLGNGKADYRWAKRHAVTEASEKAPMST

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=569, Percent_Identity=24.6045694200351, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI38505220, Length=518, Percent_Identity=23.7451737451737, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI187761345, Length=397, Percent_Identity=25.9445843828715, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI187761343, Length=397, Percent_Identity=25.9445843828715, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI115511026, Length=369, Percent_Identity=23.8482384823848, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI122937307, Length=370, Percent_Identity=22.7027027027027, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788107, Length=569, Percent_Identity=24.9560632688928, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI145693145, Length=353, Percent_Identity=26.0623229461756, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI221142682, Length=547, Percent_Identity=22.8519195612431, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790505, Length=412, Percent_Identity=22.8155339805825, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17531443, Length=366, Percent_Identity=24.3169398907104, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=355, Percent_Identity=28.4507042253521, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=394, Percent_Identity=23.6040609137056, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994694, Length=394, Percent_Identity=23.6040609137056, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994690, Length=394, Percent_Identity=23.6040609137056, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=397, Percent_Identity=23.1738035264484, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=495, Percent_Identity=22.8282828282828, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24581924, Length=353, Percent_Identity=28.0453257790368, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21355181, Length=489, Percent_Identity=22.2903885480573, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58783; Mature: 58652

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEWTIGAVLDEIADVIPDRTMTVCGDRRSTFAESADRTRRLANFLSGNGLGVHRERAVL
CCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
QNWECGQDRVALVMHNDLYPDMVVGCLKARTVPVNVNYHYTPREVGELFDYLRPRAVIYH
CCCCCCCCCEEEEEECCCCHHHHHHHHHCCEEEEEEEEEECHHHHHHHHHHHCCCEEEEE
RGLGPKFADVLGRGDVDLLIAVDDGSEAAQLPGAVSLDDALAQGDTGHPAPGSPDDLLMI
CCCCCHHHHHHCCCCEEEEEEECCCCCHHHCCCCCCHHHHHHCCCCCCCCCCCCCCEEEE
CTGGTTGRPKGVLWRQSDIYVSSMVGADHACAQEIRDKVSGAAGAPWFAVSPLMHAAGMW
EECCCCCCCCCEEEECCCEEEEHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TAFAAIMAGTTVVLYDTGKKLDPRSVWETAQRERVGMMTMVGDAYAAPLVAELQRGSYDL
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHHCCCCCH
SSLYAIGTGGAATNPKYQQALLELLPHITLINGYGSSETGNMGFGHSRTGTRTDTFTLRE
HHEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEC
GGLVLAEDYSRFLSPGEPQLGWVAREGRIPLGYFDDPDATRKTFPVIDGKQVVISGDRAA
CCEEEEEHHHHHCCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCEECCCEEEEECCCEE
LEPDGTLRLFGRDSLVVNTGGEKVFVEEVEEVLRAHPAVADALVVGRPSGRWGEEIVALV
ECCCCCEEEEECCEEEEECCCCEEHHHHHHHHHHHCCCHHHEEEEECCCCCHHHHHHHHH
ELRAGTGAAADELHAHCTSRLARFKAPKEFLFVAAVQRLGNGKADYRWAKRHAVTEASEK
HCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
APMST
CCCCC
>Mature Secondary Structure 
SEWTIGAVLDEIADVIPDRTMTVCGDRRSTFAESADRTRRLANFLSGNGLGVHRERAVL
CCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
QNWECGQDRVALVMHNDLYPDMVVGCLKARTVPVNVNYHYTPREVGELFDYLRPRAVIYH
CCCCCCCCCEEEEEECCCCHHHHHHHHHCCEEEEEEEEEECHHHHHHHHHHHCCCEEEEE
RGLGPKFADVLGRGDVDLLIAVDDGSEAAQLPGAVSLDDALAQGDTGHPAPGSPDDLLMI
CCCCCHHHHHHCCCCEEEEEEECCCCCHHHCCCCCCHHHHHHCCCCCCCCCCCCCCEEEE
CTGGTTGRPKGVLWRQSDIYVSSMVGADHACAQEIRDKVSGAAGAPWFAVSPLMHAAGMW
EECCCCCCCCCEEEECCCEEEEHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TAFAAIMAGTTVVLYDTGKKLDPRSVWETAQRERVGMMTMVGDAYAAPLVAELQRGSYDL
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHHCCCCCH
SSLYAIGTGGAATNPKYQQALLELLPHITLINGYGSSETGNMGFGHSRTGTRTDTFTLRE
HHEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEC
GGLVLAEDYSRFLSPGEPQLGWVAREGRIPLGYFDDPDATRKTFPVIDGKQVVISGDRAA
CCEEEEEHHHHHCCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCEECCCEEEEECCCEE
LEPDGTLRLFGRDSLVVNTGGEKVFVEEVEEVLRAHPAVADALVVGRPSGRWGEEIVALV
ECCCCCEEEEECCEEEEECCCCEEHHHHHHHHHHHCCCHHHEEEEECCCCCHHHHHHHHH
ELRAGTGAAADELHAHCTSRLARFKAPKEFLFVAAVQRLGNGKADYRWAKRHAVTEASEK
HCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
APMST
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]