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The map label for this gene is citE

Identifier: 41408408

GI number: 41408408

Start: 2590380

End: 2591201

Strand: Reverse

Name: citE

Synonym: MAP2310c

Alternate gene names: 41408408

Gene position: 2591201-2590380 (Counterclockwise)

Preceding gene: 41408409

Following gene: 41408407

Centisome position: 53.65

GC content: 73.48

Gene sequence:

>822_bases
GTGAACCCGCACGCCGCCGGTCCGGCATGGCTGTTCTGCCCGGCCGACCGTCCCGAGCGTTTCGCCAAGGCAGCCGCGGC
CGCCGACGTGGTGATCCTCGACCTGGAGGACGGCGTGGCCGAGGCGGACAAGCCCGCCGCCCGCAAGGCCTTGCTGGAGA
CGCCGCTGGACCCCGAGCGCACCGTGGTGCGGGTCAACGCCGCCGACACCGACGAGTACGCCCGCGATCTGGAGGCGTTG
GCCGGTACCGCGTACACCACGGTGATGCTGTCCAAGACAGAATCGGCGGCCCAGGTGCAGACCCTGGCGCCGCGCGAGGT
GATCGCGCTGCTGGAAACCCCGCGCGGGGCGGTGTTCGCGACCGAGATCGCGGCGGCCCAGAACACGGTGGCGCTGATGT
GGGGCGCCGAGGACCTGGTCGCCACGCTCGGCGGCAGCTCGAGCCGCCGGGCCGACGGCAGCTACCGGGACGTCGCCCAC
CACGTGCGCTCGACCGCCCTGCTGACCGCGTCGGCCTTCGGCCGGGCCGCGCTCGACGCCGTCCACTTGGACATCCGCGA
CCTCGACGGGTTGCGGGCCGAGGCCGAAGACGCCGTCGCGCTGGGCTTTGCCGGAACGGTGTGCATCCACCCGAGCCAGG
TTCCGGTGGTGCGCCAGGCGTACCGCCCGCCCGAGGAGAAGCTGGACTGGGCGCGACGGGTGCTGGCGGCCGCCCGCAGC
GAACGCGGGGTGTTCGCGTTCGAGGGACAGATGGTCGACTCGCCGGTGCTCAAGCACGCCCAGATGATGCTGCGCCGGGC
GGGTGAGACGGCCGCCGGCTGA

Upstream 100 bases:

>100_bases
TCACCCTCGAGCACACCGGGCGCAACCAGCACGGCGACGTCGTGGCCCGCGCCGTGCGCACCACGCTGGTGCAGAAGCGC
CCAAGCGACCAGGAGGCTCC

Downstream 100 bases:

>100_bases
CCGTCCCGCCGCGGCCGGCCCGCCCCGCTGAGCGGCGACACGTCCGGGCCGTCAGTGCAATCAACGCGGTCTCTTCTGAT
TCGGGCGCATAATGGTGAGT

Product: hypothetical protein

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNPHAAGPAWLFCPADRPERFAKAAAAADVVILDLEDGVAEADKPAARKALLETPLDPERTVVRVNAADTDEYARDLEAL
AGTAYTTVMLSKTESAAQVQTLAPREVIALLETPRGAVFATEIAAAQNTVALMWGAEDLVATLGGSSSRRADGSYRDVAH
HVRSTALLTASAFGRAALDAVHLDIRDLDGLRAEAEDAVALGFAGTVCIHPSQVPVVRQAYRPPEEKLDWARRVLAAARS
ERGVFAFEGQMVDSPVLKHAQMMLRRAGETAAG

Sequences:

>Translated_273_residues
MNPHAAGPAWLFCPADRPERFAKAAAAADVVILDLEDGVAEADKPAARKALLETPLDPERTVVRVNAADTDEYARDLEAL
AGTAYTTVMLSKTESAAQVQTLAPREVIALLETPRGAVFATEIAAAQNTVALMWGAEDLVATLGGSSSRRADGSYRDVAH
HVRSTALLTASAFGRAALDAVHLDIRDLDGLRAEAEDAVALGFAGTVCIHPSQVPVVRQAYRPPEEKLDWARRVLAAARS
ERGVFAFEGQMVDSPVLKHAQMMLRRAGETAAG
>Mature_273_residues
MNPHAAGPAWLFCPADRPERFAKAAAAADVVILDLEDGVAEADKPAARKALLETPLDPERTVVRVNAADTDEYARDLEAL
AGTAYTTVMLSKTESAAQVQTLAPREVIALLETPRGAVFATEIAAAQNTVALMWGAEDLVATLGGSSSRRADGSYRDVAH
HVRSTALLTASAFGRAALDAVHLDIRDLDGLRAEAEDAVALGFAGTVCIHPSQVPVVRQAYRPPEEKLDWARRVLAAARS
ERGVFAFEGQMVDSPVLKHAQMMLRRAGETAAG

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=287, Percent_Identity=31.3588850174216, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI87081764, Length=265, Percent_Identity=34.3396226415094, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557458, Length=298, Percent_Identity=29.5302013422819, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 29032; Mature: 29032

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPHAAGPAWLFCPADRPERFAKAAAAADVVILDLEDGVAEADKPAARKALLETPLDPER
CCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCC
TVVRVNAADTDEYARDLEALAGTAYTTVMLSKTESAAQVQTLAPREVIALLETPRGAVFA
EEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCHHHHHHHHCCCCCCEEE
TEIAAAQNTVALMWGAEDLVATLGGSSSRRADGSYRDVAHHVRSTALLTASAFGRAALDA
HHHHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHH
VHLDIRDLDGLRAEAEDAVALGFAGTVCIHPSQVPVVRQAYRPPEEKLDWARRVLAAARS
HEEEHHHCCCCCCCCCCEEEEECCCEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHC
ERGVFAFEGQMVDSPVLKHAQMMLRRAGETAAG
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPHAAGPAWLFCPADRPERFAKAAAAADVVILDLEDGVAEADKPAARKALLETPLDPER
CCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCC
TVVRVNAADTDEYARDLEALAGTAYTTVMLSKTESAAQVQTLAPREVIALLETPRGAVFA
EEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCHHHHHHHHCCCCCCEEE
TEIAAAQNTVALMWGAEDLVATLGGSSSRRADGSYRDVAHHVRSTALLTASAFGRAALDA
HHHHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHH
VHLDIRDLDGLRAEAEDAVALGFAGTVCIHPSQVPVVRQAYRPPEEKLDWARRVLAAARS
HEEEHHHCCCCCCCCCCEEEEECCCEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHC
ERGVFAFEGQMVDSPVLKHAQMMLRRAGETAAG
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]