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The map label for this gene is proB

Identifier: 41408361

GI number: 41408361

Start: 2527447

End: 2528550

Strand: Reverse

Name: proB

Synonym: MAP2263c

Alternate gene names: 41408361

Gene position: 2528550-2527447 (Counterclockwise)

Preceding gene: 41408362

Following gene: 41408359

Centisome position: 52.35

GC content: 73.55

Gene sequence:

>1104_bases
GTGACCGCCCACCGCGACGCCATCCGCACGGCGCGCAGCATGGTGGTCAAGGTGGGCACCAACGCGTTGACCACCCCGGC
CGGGGTGTTCGACGCCGGCCGGCTGGCCGGGCTCGCCGACGCCATCGAGGCGCGGATGAAGGCCGGCACCGACGTCGTCA
TCGTCTCGTCGGGTGCCATCGCCGCGGGCATCGAGCCGCTCGGATTGTCCCGTCGCCCCAGGGATCTGGCGACCAAGCAG
GCCGCGGCCAGCGTCGGTCAGGTGGCACTGGTGAATTCCTGGAGCGCGGCGTTCGCCCGCTACGGCCGCACCGTCGGGCA
GGTGCTGCTGAGCGCGCACGACATCTCGATGCGCGCCCAGCACACCAACGCCCAGCGCACCCTGGATCGGCTGCGCGCCC
TGCATGCGGTGGCGATCGTCAACGAGAACGACACCGTGGCCACCAACGAGATCCGGTTCGGCGACAACGACCGGCTCTCG
GCGCTGGTGGCCCACCTGGTCGGCGCCGAGGCGCTGGTCTTGCTGTCCGACATCGACGGCCTTTACGACTCGGACCCGCG
AAAGACCAAGGGAGCCAAGTTCATTCCGGAGGTGACGGGCGCGGAGGATCTGGCCGGCGTGGTCGCCGGCCCGGGCAGCG
ACCTGGGCACCGGCGGCATGACGTCGAAGATGTCCTCGGCGCTGCTGGCCGCCGACGCCGGGGTGCCCGTGCTGCTGGCC
GCCGCGGCCGACGCCGCCAGCGCGCTCACCGACGCTTCGGTCGGCACGGTGTTCGCCGCCCGGCCGGTGCGGATGTCGGC
CCGGCGGTTCTGGGTGCGCTACGCCGCCGAGGCGGCCGGCGCGCTGACCCTGGACGAGGGCGCGGTGCGCGCCGTGGTGC
ATCAGCGCCGCTCGCTGCTGCCCGCGGGCATCACCGCGGTGTCCGGCCGGTTCTACGCCGGCGACGTCGTGGAACTGCGC
GGGCCGGACGCGGTGCCGGTGGCCCGCGGCGTGGTGGCCTACGACACCACCGAACTGGCGACCATGATGGGGCGCTCGAC
CAGTGAGCTGCCCGGCGAGCTGCGCAGGCCCGCGGTGCACGCCGACGACCTGGTGGCGGTGTGA

Upstream 100 bases:

>100_bases
GCACCGAGCGGGTCGGCGCCGCCGAGCGCAAGGCCGCCCGCAGGCAGCGCCGCACCGGCGACGATGCAGAGCGCGGAACC
ACAGAACGCGGTGAGAACAC

Downstream 100 bases:

>100_bases
GCGGTGCCGTGCGGTCAGATCCCGGAGGCGGGCTTGAGATACAGTGCGCCCCAAACGATTTCGGCCAGCGTGTCGGCCAG
CTCCGCGTCGTAGGCGGGCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTAHRDAIRTARSMVVKVGTNALTTPAGVFDAGRLAGLADAIEARMKAGTDVVIVSSGAIAAGIEPLGLSRRPRDLATKQ
AAASVGQVALVNSWSAAFARYGRTVGQVLLSAHDISMRAQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLS
ALVAHLVGAEALVLLSDIDGLYDSDPRKTKGAKFIPEVTGAEDLAGVVAGPGSDLGTGGMTSKMSSALLAADAGVPVLLA
AAADAASALTDASVGTVFAARPVRMSARRFWVRYAAEAAGALTLDEGAVRAVVHQRRSLLPAGITAVSGRFYAGDVVELR
GPDAVPVARGVVAYDTTELATMMGRSTSELPGELRRPAVHADDLVAV

Sequences:

>Translated_367_residues
MTAHRDAIRTARSMVVKVGTNALTTPAGVFDAGRLAGLADAIEARMKAGTDVVIVSSGAIAAGIEPLGLSRRPRDLATKQ
AAASVGQVALVNSWSAAFARYGRTVGQVLLSAHDISMRAQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLS
ALVAHLVGAEALVLLSDIDGLYDSDPRKTKGAKFIPEVTGAEDLAGVVAGPGSDLGTGGMTSKMSSALLAADAGVPVLLA
AAADAASALTDASVGTVFAARPVRMSARRFWVRYAAEAAGALTLDEGAVRAVVHQRRSLLPAGITAVSGRFYAGDVVELR
GPDAVPVARGVVAYDTTELATMMGRSTSELPGELRRPAVHADDLVAV
>Mature_366_residues
TAHRDAIRTARSMVVKVGTNALTTPAGVFDAGRLAGLADAIEARMKAGTDVVIVSSGAIAAGIEPLGLSRRPRDLATKQA
AASVGQVALVNSWSAAFARYGRTVGQVLLSAHDISMRAQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSA
LVAHLVGAEALVLLSDIDGLYDSDPRKTKGAKFIPEVTGAEDLAGVVAGPGSDLGTGGMTSKMSSALLAADAGVPVLLAA
AADAASALTDASVGTVFAARPVRMSARRFWVRYAAEAAGALTLDEGAVRAVVHQRRSLLPAGITAVSGRFYAGDVVELRG
PDAVPVARGVVAYDTTELATMMGRSTSELPGELRRPAVHADDLVAV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=295, Percent_Identity=30.1694915254237, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI62912457, Length=293, Percent_Identity=30.0341296928328, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=39.1184573002755, Blast_Score=229, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17569967, Length=275, Percent_Identity=32.7272727272727, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=275, Percent_Identity=32.7272727272727, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=403, Percent_Identity=30.0248138957816, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=356, Percent_Identity=31.7415730337079, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=32.6460481099656, Blast_Score=121, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 37890; Mature: 37758

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHRDAIRTARSMVVKVGTNALTTPAGVFDAGRLAGLADAIEARMKAGTDVVIVSSGAI
CCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCC
AAGIEPLGLSRRPRDLATKQAAASVGQVALVNSWSAAFARYGRTVGQVLLSAHDISMRAQ
EECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGAEALVLLSDIDG
HCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCHHHHHHHHHHHCHHHEEHHHHCCC
LYDSDPRKTKGAKFIPEVTGAEDLAGVVAGPGSDLGTGGMTSKMSSALLAADAGVPVLLA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
AAADAASALTDASVGTVFAARPVRMSARRFWVRYAAEAAGALTLDEGAVRAVVHQRRSLL
EHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCC
PAGITAVSGRFYAGDVVELRGPDAVPVARGVVAYDTTELATMMGRSTSELPGELRRPAVH
CCCHHHHCCCEEECCEEEECCCCCCHHHCCEEEECHHHHHHHHCCCCCCCCHHHHCCCCC
ADDLVAV
CCCEECC
>Mature Secondary Structure 
TAHRDAIRTARSMVVKVGTNALTTPAGVFDAGRLAGLADAIEARMKAGTDVVIVSSGAI
CCHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCC
AAGIEPLGLSRRPRDLATKQAAASVGQVALVNSWSAAFARYGRTVGQVLLSAHDISMRAQ
EECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGAEALVLLSDIDG
HCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCHHHHHHHHHHHCHHHEEHHHHCCC
LYDSDPRKTKGAKFIPEVTGAEDLAGVVAGPGSDLGTGGMTSKMSSALLAADAGVPVLLA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
AAADAASALTDASVGTVFAARPVRMSARRFWVRYAAEAAGALTLDEGAVRAVVHQRRSLL
EHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCC
PAGITAVSGRFYAGDVVELRGPDAVPVARGVVAYDTTELATMMGRSTSELPGELRRPAVH
CCCHHHHCCCEEECCEEEECCCCCCHHHCCEEEECHHHHHHHHCCCCCCCCHHHHCCCCC
ADDLVAV
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA