| Definition | Mycobacterium avium subsp. paratuberculosis K-10, complete genome. |
|---|---|
| Accession | NC_002944 |
| Length | 4,829,781 |
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The map label for this gene is nadE
Identifier: 41408356
GI number: 41408356
Start: 2521878
End: 2523920
Strand: Reverse
Name: nadE
Synonym: MAP2258c
Alternate gene names: 41408356
Gene position: 2523920-2521878 (Counterclockwise)
Preceding gene: 41408359
Following gene: 41408350
Centisome position: 52.26
GC content: 67.3
Gene sequence:
>2043_bases ATGGACTTTTACAACGCTTACAGCCAGGGCTTCGCCCGCGTCGCCGCGTGCACGCATCGCACCGTCATCGGTGACCCCGC CGCGAACGCCGAGTCGGTGCTGCGGCTGGCCCGCGCCTGCCACGACGACAGCGCGGCGCTGGCCGTCTTCCCGGAACTGA CGCTGTCCGGGTATTCCATCGAAGACATCGTGCTGCAGGACCTGTTGCTCGACGACGTGGAGCAGGCCATCGCCGCGATC GTGGCGGCCTCGGCCGATCTGTTGCCGGTCTTGGTGGTCGGGGCCCCGGTGCGGCACCGGCACCGGATCTACAACGCCGC GCTGGTGATCCATCGCGGCGCGCTGCTCGGTGTGGTGCCGAAGTCCTACCTGCCCACCTATCGGGAGTTCTACGAGCGCC GTCAGATCGCGCCGGGCGACGACGAGCGCGGCACCGTCCGCGTCGCCGGCCTCGAGGCGCCGTTCGGCCCGGATCTGCTC TTCGCCGCCTCCGACCTGCCCGGTTTCGTGCTGCACGTGGAGATCTCCGAGGACATGTTCGTGCCGATTCCGCCCAGCGC GCAGGCCGCGCTGGCCGGCGCGACGGTGCTGGCCAACCTGTCCGGCAGCCCCATCACGATCGGACGTGCCGAGGACCGTT GCCTGCTGGCGCGTTCCGCGTCCTCGCGGTGCTTGGCCGCCTACGTGTATTCCGCTGCCGGCGAAGGGGAATCCACCACC GATCTGGCCTGGGACGGTCAGACCATGGTGTGGGAAAACGGTGTGCTGCTGGCGATGTCGGAGCGGTTCCCGAAGGGGGA GCGGCGCAGCATCGCCGACATCGACACCGAATTGCTTCGCTCGGAACGACTTCGGATGGGCACCTTCGACGACAACCGGC GCCATCACCGGATCGCGTCGGAGTCGTTCCGGCGCATCGAGTTCCGGCTCGACCCACCGGCCGGTGACATCGGCTTGCGG CGTGAGATCGAGCGCTTTCCCTTCGTGCCCGCCGATCGCGAACGGCTGCAACAGGATTGCTTCGAGGCCTACAACATTCA GGTCGCCGGGCTGGAACAGCGGCTGCGCGCCCTGGACTACCCCAAGCTCGTCATCGGGATTTCCGGCGGGCTGGACTCGA CACACGCGTTGATCGTCGCCGCCCGGGCCATGGACCGCGAACAACGGCCGCGCAGCGACATTCTGGCGTTCACGTTGCCC GGCTTCGCCACTGGGGACCGCACCAAACGCAACGCGATCGAGCTGTGCCGCGCACTCGGGGTGACGTTCTCCGAGATCGA CATCCGCGAGACCGCCCAGCTGATGCTCAAGGAGATGGACCATCCCTTCGCGCGCGGCGAAAAGGTGTACGACGTCACCT TCGAAAACGTGCAGGCCGGCTTGCGCACCGATTATCTGTTCCGGCTGGCCAACCAGCGCGGCGGCATCGTGCTGGGCACC GGCGATCTCTCCGAACTGGGGCTGGGCTGGTCGACCTACGGTGTCGGCGACCAGATGTCGCACTACAACGTCAACGCCGG GGTGCCCAAGACGCTGATCCAGCACCTGATCCGCTGGGTCATCTCGTCGGAGGAGTTCGCGCCGGAGGTCGGCGCGGTGC TGCAGTCGGTGCTCGACACCGAGATCACCCCGGAGCTGGTGCCCAGCGGCGAGGAGGAGGAACTGCAGAGCAGCGAGGCC AAGGTGGGTCCGTTTGCGCTGCAGGACTTTTCGCTGTTCCATGTGCTGCGCTACGGGTTCAGGCCGTCCAAGATCGCGTT CCTGGCCTGGCATGCGTGGAGCGATCCCGAGCAGGGCAACTGGCCGCCCGGCTTCCCCGAGGACAAGCGACCGTCCTACT CGCTCAAGGAAATCCGGCACTGGCTGCAGGTCTTCGTACAGCGGTTCTATTCGTTCAGCCAGTTCAAGCGCTCGGCGCTG CCCAACGGCCCCAAGGTGTCGCACGGCGGTGCGCTCTCGCCGCGCGGCGACTGGCGTGCCCCGTCGGACATGTCGGCGCG CATCTGGCTCGACGAGATCGAACGCGAAATCCCCGAGGACTAG
Upstream 100 bases:
>100_bases GAATTCATCATGTCGACCACCAACACAGCAGTGTCGCTCATGCCGTCCGGGATACCCGGCGGCCAGCAGTTGACACGCCT GGGGCGACAATGGAAGGGCG
Downstream 100 bases:
>100_bases CAACGGCGGCGGTGGGCCCGCCCGGGTTCGCCGAGCGGGCCCCGAACCTCACGCCCTGGCGCGCCGCCAGCCAAAGTATT GCAGCTCGGCGAACACCAGC
Product: NAD synthetase
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]
Number of amino acids: Translated: 680; Mature: 680
Protein sequence:
>680_residues MDFYNAYSQGFARVAACTHRTVIGDPAANAESVLRLARACHDDSAALAVFPELTLSGYSIEDIVLQDLLLDDVEQAIAAI VAASADLLPVLVVGAPVRHRHRIYNAALVIHRGALLGVVPKSYLPTYREFYERRQIAPGDDERGTVRVAGLEAPFGPDLL FAASDLPGFVLHVEISEDMFVPIPPSAQAALAGATVLANLSGSPITIGRAEDRCLLARSASSRCLAAYVYSAAGEGESTT DLAWDGQTMVWENGVLLAMSERFPKGERRSIADIDTELLRSERLRMGTFDDNRRHHRIASESFRRIEFRLDPPAGDIGLR REIERFPFVPADRERLQQDCFEAYNIQVAGLEQRLRALDYPKLVIGISGGLDSTHALIVAARAMDREQRPRSDILAFTLP GFATGDRTKRNAIELCRALGVTFSEIDIRETAQLMLKEMDHPFARGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT GDLSELGLGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISSEEFAPEVGAVLQSVLDTEITPELVPSGEEEELQSSEA KVGPFALQDFSLFHVLRYGFRPSKIAFLAWHAWSDPEQGNWPPGFPEDKRPSYSLKEIRHWLQVFVQRFYSFSQFKRSAL PNGPKVSHGGALSPRGDWRAPSDMSARIWLDEIEREIPED
Sequences:
>Translated_680_residues MDFYNAYSQGFARVAACTHRTVIGDPAANAESVLRLARACHDDSAALAVFPELTLSGYSIEDIVLQDLLLDDVEQAIAAI VAASADLLPVLVVGAPVRHRHRIYNAALVIHRGALLGVVPKSYLPTYREFYERRQIAPGDDERGTVRVAGLEAPFGPDLL FAASDLPGFVLHVEISEDMFVPIPPSAQAALAGATVLANLSGSPITIGRAEDRCLLARSASSRCLAAYVYSAAGEGESTT DLAWDGQTMVWENGVLLAMSERFPKGERRSIADIDTELLRSERLRMGTFDDNRRHHRIASESFRRIEFRLDPPAGDIGLR REIERFPFVPADRERLQQDCFEAYNIQVAGLEQRLRALDYPKLVIGISGGLDSTHALIVAARAMDREQRPRSDILAFTLP GFATGDRTKRNAIELCRALGVTFSEIDIRETAQLMLKEMDHPFARGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT GDLSELGLGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISSEEFAPEVGAVLQSVLDTEITPELVPSGEEEELQSSEA KVGPFALQDFSLFHVLRYGFRPSKIAFLAWHAWSDPEQGNWPPGFPEDKRPSYSLKEIRHWLQVFVQRFYSFSQFKRSAL PNGPKVSHGGALSPRGDWRAPSDMSARIWLDEIEREIPED >Mature_680_residues MDFYNAYSQGFARVAACTHRTVIGDPAANAESVLRLARACHDDSAALAVFPELTLSGYSIEDIVLQDLLLDDVEQAIAAI VAASADLLPVLVVGAPVRHRHRIYNAALVIHRGALLGVVPKSYLPTYREFYERRQIAPGDDERGTVRVAGLEAPFGPDLL FAASDLPGFVLHVEISEDMFVPIPPSAQAALAGATVLANLSGSPITIGRAEDRCLLARSASSRCLAAYVYSAAGEGESTT DLAWDGQTMVWENGVLLAMSERFPKGERRSIADIDTELLRSERLRMGTFDDNRRHHRIASESFRRIEFRLDPPAGDIGLR REIERFPFVPADRERLQQDCFEAYNIQVAGLEQRLRALDYPKLVIGISGGLDSTHALIVAARAMDREQRPRSDILAFTLP GFATGDRTKRNAIELCRALGVTFSEIDIRETAQLMLKEMDHPFARGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT GDLSELGLGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISSEEFAPEVGAVLQSVLDTEITPELVPSGEEEELQSSEA KVGPFALQDFSLFHVLRYGFRPSKIAFLAWHAWSDPEQGNWPPGFPEDKRPSYSLKEIRHWLQVFVQRFYSFSQFKRSAL PNGPKVSHGGALSPRGDWRAPSDMSARIWLDEIEREIPED
Specific function: Can use both glutamine or ammonia as a nitrogen source [H]
COG id: COG0171
COG function: function code H; NAD synthase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
Organism=Homo sapiens, GI41393551, Length=624, Percent_Identity=22.9166666666667, Blast_Score=79, Evalue=2e-14, Organism=Saccharomyces cerevisiae, GI6321865, Length=654, Percent_Identity=23.0886850152905, Blast_Score=100, Evalue=1e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 75503; Mature: 75503
Theoretical pI: Translated: 5.14; Mature: 5.14
Prosite motif: PS00591 GLYCOSYL_HYDROL_F10 ; PS50263 CN_HYDROLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDFYNAYSQGFARVAACTHRTVIGDPAANAESVLRLARACHDDSAALAVFPELTLSGYSI CCCHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCEECCCCH EDIVLQDLLLDDVEQAIAAIVAASADLLPVLVVGAPVRHRHRIYNAALVIHRGALLGVVP HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHEEECCCCEECCC KSYLPTYREFYERRQIAPGDDERGTVRVAGLEAPFGPDLLFAASDLPGFVLHVEISEDMF HHHCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEECCCEE VPIPPSAQAALAGATVLANLSGSPITIGRAEDRCLLARSASSRCLAAYVYSAAGEGESTT EECCCCHHHHHHHHHHEEECCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCC DLAWDGQTMVWENGVLLAMSERFPKGERRSIADIDTELLRSERLRMGTFDDNRRHHRIAS EEEECCCEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH ESFRRIEFRLDPPAGDIGLRREIERFPFVPADRERLQQDCFEAYNIQVAGLEQRLRALDY HHHEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCEEEECHHHHHHHCCC PKLVIGISGGLDSTHALIVAARAMDREQRPRSDILAFTLPGFATGDRTKRNAIELCRALG CEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHC VTFSEIDIRETAQLMLKEMDHPFARGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT CCHHHCCHHHHHHHHHHHCCCCHHCCCEEEEEEHHHHHHCHHHHHHHHHHCCCCCEEEEC GDLSELGLGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISSEEFAPEVGAVLQSVLDT CCHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHCC EITPELVPSGEEEELQSSEAKVGPFALQDFSLFHVLRYGFRPSKIAFLAWHAWSDPEQGN CCCHHHCCCCCHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC WPPGFPEDKRPSYSLKEIRHWLQVFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRA CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC PSDMSARIWLDEIEREIPED CCCCCCEEEHHHHHHHCCCC >Mature Secondary Structure MDFYNAYSQGFARVAACTHRTVIGDPAANAESVLRLARACHDDSAALAVFPELTLSGYSI CCCHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCEECCCCH EDIVLQDLLLDDVEQAIAAIVAASADLLPVLVVGAPVRHRHRIYNAALVIHRGALLGVVP HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHEEECCCCEECCC KSYLPTYREFYERRQIAPGDDERGTVRVAGLEAPFGPDLLFAASDLPGFVLHVEISEDMF HHHCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEECCCEE VPIPPSAQAALAGATVLANLSGSPITIGRAEDRCLLARSASSRCLAAYVYSAAGEGESTT EECCCCHHHHHHHHHHEEECCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCC DLAWDGQTMVWENGVLLAMSERFPKGERRSIADIDTELLRSERLRMGTFDDNRRHHRIAS EEEECCCEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH ESFRRIEFRLDPPAGDIGLRREIERFPFVPADRERLQQDCFEAYNIQVAGLEQRLRALDY HHHEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCEEEECHHHHHHHCCC PKLVIGISGGLDSTHALIVAARAMDREQRPRSDILAFTLPGFATGDRTKRNAIELCRALG CEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHC VTFSEIDIRETAQLMLKEMDHPFARGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT CCHHHCCHHHHHHHHHHHCCCCHHCCCEEEEEEHHHHHHCHHHHHHHHHHCCCCCEEEEC GDLSELGLGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISSEEFAPEVGAVLQSVLDT CCHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHCC EITPELVPSGEEEELQSSEAKVGPFALQDFSLFHVLRYGFRPSKIAFLAWHAWSDPEQGN CCCHHHCCCCCHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC WPPGFPEDKRPSYSLKEIRHWLQVFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRA CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC PSDMSARIWLDEIEREIPED CCCCCCEEEHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11234002 [H]