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The map label for this gene is fadD21_1

Identifier: 41408333

GI number: 41408333

Start: 2497365

End: 2499101

Strand: Direct

Name: fadD21_1

Synonym: MAP2235

Alternate gene names: 41408333

Gene position: 2497365-2499101 (Clockwise)

Preceding gene: 41408332

Following gene: 41408334

Centisome position: 51.71

GC content: 65.92

Gene sequence:

>1737_bases
ATGTCCCAGTCATCTATTCTGTCGATGCTGCACGCACGGGCCAGCCTGCGTCCCGCTGACATCGCGTTCACCTTCACCGA
TTACGAGCGCGACTGGGCGGGTGTTCGGGAGAGCCTCACCTGGTCGCAGTTGTCGCGCAGAACCATCAACGTGGCGCGTG
AGCTTCACCTTCATGGTTCGGTGGGAGACCGGGCGGTAATCCTTGCGCCCCAAAGCCTCGACTACATCGCGGCGTTCCTC
GGCTCGATGCAGGCCGGGCTCGTCGCGGTTCCACTCCCGTTGCCGCACCGCGGCTCGAGTCATGAGCGGGTCAGCGCGGT
GCTTGCCGACACCTCGCCGTCCGTCGTCCTGACGACGTCCGCGGTCGCCGAAGACATCACCGAATACGTGGACAAGGCCT
GCCTGGACGCGGTTCCGAAGATCGTCGAGATCGATTCGCTGAACCTCGACGTCGACGGCGGACCACACGTGCGGGCGGCC
GACCTGCCCAGCGTCGCCTATCTGCAGTACAGCTCGGGTTCGACGCGGCAGCCCACCGGGGTGATGATCTCGCACCGCAA
CCTCAAGGTGAATTTCGAACAGTTGATGCGCGGCTTTTTCGCCGACGCCCACGTCAAGGCCCCCTCGGATCTAACCATCG
TGTCCTGGCTGCCCTTCTACCACGACATGGGTTTGGTGCTCGGGGTCTGCGCCCCGGTCCTGGGTGGCTACCGGGGCGAG
TTGAGCAGTCCGCTTTCGTTTTTGGAGCGGCCGGCCCGATGGGTGCAGGCCCTGGCCAGCAGCCCGCACGCGTGGTCGGC
GGCACCTAACTTCGCCTTCGACTTAGCGGCCCGCAAGACAACCGACGGCGACCTGGCCGGCCTGGACCTCGGTGGGGTGC
TGGGCATCATCAGCGGCGCCGAACGGGTGGAGCCGGCCACCTTGCAACGCTTCGTTGATCGGTTCGCGCACTTCAATTTT
CGCGACGAGATGGTGCGTCCGTCGTACGGCTTGGCGGAGGCGACCGTCTTCGCGGCGGCCGGCACCTGGAATGAGTCGTC
GGGAGCGGTCCGCTTCGATACCGACGAACTGTCCGCCGGACGCGCCCGGCGGTGCGCGGCCGGGTCCGGCACCGCGTTGG
TCAAATACCAAGTGCCGCAGTCACCGACGCTGCGCATCGTCGACGGCGACACCCAGCGGGAGTGCCCGCCCGGCGTGGTC
GGCGAGATCTGGGTGCATGGCGAAAACGTGGCCGACGGCTACTGGCGCAAGCTGCCGCACGAACAGTCCTGCTTCGGCGC
CACGCTGCGGGATCCCTCGCCCGGCACGCCCGGCGGGCCCTGGCTGAAAACCGGCGATCAGGGTTTCCTTTTCGACGGCG
CGCTTTTCATCGTCGGCCGCATCAAGGACCTGCTGATCATCAACGGCCGCAATCACTATCCCGAGGACATCGAGGCGACC
GTGCACGAGATCACCCGCGGCCGGGTCGCGGCGATATCGGTCCCGGCGAACGGCACCGAAAAGCTGGTCACCATCATCGA
ATTCAAAGAGCGCAGCGATTCGGCGGACACGTCGCACCGAATCGCGAGCGTCAAAAGCGATGTCACGGCGGCGATATCCA
ACGCGCACGGCCTGAACGTCGACGACGTCGTGCTGGTATCGCCCGGATCCATTCCCACCACGACGAGCGGCAAGATCCGC
CGCGCCGGCTGTATCGAGCAGTACCGTCAGCGGCAATTCACCCGGTTGGACAGCTGA

Upstream 100 bases:

>100_bases
GGCCGGGGCCATGTCCGTGTTGTCGCACGGCGCGGGCGAAGCCGACCACTGACTCCGAGCACGAAAAACCGACTATCAGT
ACGGACGTGATGTGATGCCT

Downstream 100 bases:

>100_bases
GGGAGGCGCTCGCCGATCGGGCCGCCTCACAGGCATATCGATGTTGATCACCGTGCTGGTCATGGCCGCGGCCGTCAGCG
TCGAACCGTTCCGCATCGGT

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSQSSILSMLHARASLRPADIAFTFTDYERDWAGVRESLTWSQLSRRTINVARELHLHGSVGDRAVILAPQSLDYIAAFL
GSMQAGLVAVPLPLPHRGSSHERVSAVLADTSPSVVLTTSAVAEDITEYVDKACLDAVPKIVEIDSLNLDVDGGPHVRAA
DLPSVAYLQYSSGSTRQPTGVMISHRNLKVNFEQLMRGFFADAHVKAPSDLTIVSWLPFYHDMGLVLGVCAPVLGGYRGE
LSSPLSFLERPARWVQALASSPHAWSAAPNFAFDLAARKTTDGDLAGLDLGGVLGIISGAERVEPATLQRFVDRFAHFNF
RDEMVRPSYGLAEATVFAAAGTWNESSGAVRFDTDELSAGRARRCAAGSGTALVKYQVPQSPTLRIVDGDTQRECPPGVV
GEIWVHGENVADGYWRKLPHEQSCFGATLRDPSPGTPGGPWLKTGDQGFLFDGALFIVGRIKDLLIINGRNHYPEDIEAT
VHEITRGRVAAISVPANGTEKLVTIIEFKERSDSADTSHRIASVKSDVTAAISNAHGLNVDDVVLVSPGSIPTTTSGKIR
RAGCIEQYRQRQFTRLDS

Sequences:

>Translated_578_residues
MSQSSILSMLHARASLRPADIAFTFTDYERDWAGVRESLTWSQLSRRTINVARELHLHGSVGDRAVILAPQSLDYIAAFL
GSMQAGLVAVPLPLPHRGSSHERVSAVLADTSPSVVLTTSAVAEDITEYVDKACLDAVPKIVEIDSLNLDVDGGPHVRAA
DLPSVAYLQYSSGSTRQPTGVMISHRNLKVNFEQLMRGFFADAHVKAPSDLTIVSWLPFYHDMGLVLGVCAPVLGGYRGE
LSSPLSFLERPARWVQALASSPHAWSAAPNFAFDLAARKTTDGDLAGLDLGGVLGIISGAERVEPATLQRFVDRFAHFNF
RDEMVRPSYGLAEATVFAAAGTWNESSGAVRFDTDELSAGRARRCAAGSGTALVKYQVPQSPTLRIVDGDTQRECPPGVV
GEIWVHGENVADGYWRKLPHEQSCFGATLRDPSPGTPGGPWLKTGDQGFLFDGALFIVGRIKDLLIINGRNHYPEDIEAT
VHEITRGRVAAISVPANGTEKLVTIIEFKERSDSADTSHRIASVKSDVTAAISNAHGLNVDDVVLVSPGSIPTTTSGKIR
RAGCIEQYRQRQFTRLDS
>Mature_577_residues
SQSSILSMLHARASLRPADIAFTFTDYERDWAGVRESLTWSQLSRRTINVARELHLHGSVGDRAVILAPQSLDYIAAFLG
SMQAGLVAVPLPLPHRGSSHERVSAVLADTSPSVVLTTSAVAEDITEYVDKACLDAVPKIVEIDSLNLDVDGGPHVRAAD
LPSVAYLQYSSGSTRQPTGVMISHRNLKVNFEQLMRGFFADAHVKAPSDLTIVSWLPFYHDMGLVLGVCAPVLGGYRGEL
SSPLSFLERPARWVQALASSPHAWSAAPNFAFDLAARKTTDGDLAGLDLGGVLGIISGAERVEPATLQRFVDRFAHFNFR
DEMVRPSYGLAEATVFAAAGTWNESSGAVRFDTDELSAGRARRCAAGSGTALVKYQVPQSPTLRIVDGDTQRECPPGVVG
EIWVHGENVADGYWRKLPHEQSCFGATLRDPSPGTPGGPWLKTGDQGFLFDGALFIVGRIKDLLIINGRNHYPEDIEATV
HEITRGRVAAISVPANGTEKLVTIIEFKERSDSADTSHRIASVKSDVTAAISNAHGLNVDDVVLVSPGSIPTTTSGKIRR
AGCIEQYRQRQFTRLDS

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=513, Percent_Identity=25.3411306042885, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI225735629, Length=511, Percent_Identity=25.8317025440313, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI45827692, Length=511, Percent_Identity=25.8317025440313, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI225735625, Length=629, Percent_Identity=24.0063593004769, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI55749758, Length=575, Percent_Identity=24.3478260869565, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788107, Length=574, Percent_Identity=25.0871080139373, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=489, Percent_Identity=24.9488752556237, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI193202679, Length=561, Percent_Identity=22.9946524064171, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24654656, Length=432, Percent_Identity=22.4537037037037, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62447; Mature: 62316

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSSILSMLHARASLRPADIAFTFTDYERDWAGVRESLTWSQLSRRTINVARELHLHGS
CCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHEEECCC
VGDRAVILAPQSLDYIAAFLGSMQAGLVAVPLPLPHRGSSHERVSAVLADTSPSVVLTTS
CCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHEEEEECCCCCEEEEHH
AVAEDITEYVDKACLDAVPKIVEIDSLNLDVDGGPHVRAADLPSVAYLQYSSGSTRQPTG
HHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCEEECCCCCEEEEEECCCCCCCCCC
VMISHRNLKVNFEQLMRGFFADAHVKAPSDLTIVSWLPFYHDMGLVLGVCAPVLGGYRGE
EEEECCCEEECHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
LSSPLSFLERPARWVQALASSPHAWSAAPNFAFDLAARKTTDGDLAGLDLGGVLGIISGA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHCCCCCCCCCCCCCHHHHHHHHCCC
ERVEPATLQRFVDRFAHFNFRDEMVRPSYGLAEATVFAAAGTWNESSGAVRFDTDELSAG
HHCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEECHHHCCCC
RARRCAAGSGTALVKYQVPQSPTLRIVDGDTQRECPPGVVGEIWVHGENVADGYWRKLPH
CCHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHCCC
EQSCFGATLRDPSPGTPGGPWLKTGDQGFLFDGALFIVGRIKDLLIINGRNHYPEDIEAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHEECCCEEEEECCCCCCCHHHHHH
VHEITRGRVAAISVPANGTEKLVTIIEFKERSDSADTSHRIASVKSDVTAAISNAHGLNV
HHHHCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DDVVLVSPGSIPTTTSGKIRRAGCIEQYRQRQFTRLDS
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQSSILSMLHARASLRPADIAFTFTDYERDWAGVRESLTWSQLSRRTINVARELHLHGS
CHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHEEECCC
VGDRAVILAPQSLDYIAAFLGSMQAGLVAVPLPLPHRGSSHERVSAVLADTSPSVVLTTS
CCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHEEEEECCCCCEEEEHH
AVAEDITEYVDKACLDAVPKIVEIDSLNLDVDGGPHVRAADLPSVAYLQYSSGSTRQPTG
HHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCEEECCCCCEEEEEECCCCCCCCCC
VMISHRNLKVNFEQLMRGFFADAHVKAPSDLTIVSWLPFYHDMGLVLGVCAPVLGGYRGE
EEEECCCEEECHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
LSSPLSFLERPARWVQALASSPHAWSAAPNFAFDLAARKTTDGDLAGLDLGGVLGIISGA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHCCCCCCCCCCCCCHHHHHHHHCCC
ERVEPATLQRFVDRFAHFNFRDEMVRPSYGLAEATVFAAAGTWNESSGAVRFDTDELSAG
HHCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEECHHHCCCC
RARRCAAGSGTALVKYQVPQSPTLRIVDGDTQRECPPGVVGEIWVHGENVADGYWRKLPH
CCHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHCCC
EQSCFGATLRDPSPGTPGGPWLKTGDQGFLFDGALFIVGRIKDLLIINGRNHYPEDIEAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHEECCCEEEEECCCCCCCHHHHHH
VHEITRGRVAAISVPANGTEKLVTIIEFKERSDSADTSHRIASVKSDVTAAISNAHGLNV
HHHHCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DDVVLVSPGSIPTTTSGKIRRAGCIEQYRQRQFTRLDS
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]