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The map label for this gene is subI

Identifier: 41408311

GI number: 41408311

Start: 2460135

End: 2461166

Strand: Reverse

Name: subI

Synonym: MAP2213c

Alternate gene names: 41408311

Gene position: 2461166-2460135 (Counterclockwise)

Preceding gene: 41408312

Following gene: 41408310

Centisome position: 50.96

GC content: 67.25

Gene sequence:

>1032_bases
ATGGACATCAGGACCGCAGCGCGCTGGCGGCCTGTTCTCGCCCTTGTTCTCACCGCCGGTGTCGTGGCCGGATGCCACGG
CGGCGCCAGCGATGCCGTCGGCGGCACCGGGCCGGCCGACGCGCGCACCAGCATCACGCTGGTCGCCTACTCGGTCCCAG
AACCCGGATGGAGCAAGATAATTCCGGCGTTCAACGCCTCCGACGAGGGCAAGGGCATCCAGGTGGTCACCTCCTACGGG
GCCTCCGGTGACCAATCCCGCGGCGTGGTGGACGGCAAACCCGCCGACGTGGTGAACTTCTCCGTCGAACCCGACATCGC
TCGCCTGGTCAAGGCCGGCAAGGTCGCCAAGGATTGGAACACCGACGCCACCAAGGGGATCCCGTTCGGCTCGGTGGTCA
CGCTGGTGGTGCGCAAGGGCAATCCGAAGCACATCAAGGATTGGGACGACCTGTTGCGGCCCGGCGTCGAGGTGATCACG
CCCAGCCCGCTCAGTTCCGGGTCGGCCAAGTGGAATCTGCTGGCGCCGTACGCCGTCAAGAGCGAGGGCGGCGCGCACGG
CGACGCCGGCGTGGACTTCATCCGGAAGTTGGTGACCGAACACGTCAAGTTGCGCCCGGGATCGGGGCGCGAGGCCACCG
ACGTCTTCGTGCAGGGCAGCGGTGACGTGTTGATCAGCTACGAGAACGAGGCCATCGCCACCGAGCGGGCGGGAAAGCCG
GTCGAGCACCTCAATCTGGCGCAGACCTTCAAGATCGACAACCCGGTCGCGGTGGTCAACACCAGCCCGCACCTGCAGGC
CGCCGTCGCGTTCAAGAACTTCCAGTACACCGCGGCGGCCCAAAAGGTTTGGGCGCAAGCCGGTTTCCGGCCGGTCGACC
CGGCCGTCGCCGCCGACTTCCGCGACCAGTATCCGGTGCCGGCGAAACTGTGGACCATCGCCGATCTGGGCGGCTGGAGC
GCCGCGGACCCGCAGCTGTTCGACAAGAACACCGGCAGCATCACCAAGATCTACACGCAGGCCACCGGATGA

Upstream 100 bases:

>100_bases
GTCTGGTTCCGGAGTTCTCGAATTCTCCTGACGTTCTAGTTGCCTTTCCGCCGAGACGGGACACCGAGATCCGCTAAGTT
CTCCCACCGGAAGGCCATCG

Downstream 100 bases:

>100_bases
CGGCCATCACTCCCGACCCGCTGGCCATCCGGCCCGAACTCGGCGGCGACCCCGGACACCAGCCGGTGCCCGGACGCGGC
CGCGGCACCACCGCGCTGCG

Product: SubI

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDIRTAARWRPVLALVLTAGVVAGCHGGASDAVGGTGPADARTSITLVAYSVPEPGWSKIIPAFNASDEGKGIQVVTSYG
ASGDQSRGVVDGKPADVVNFSVEPDIARLVKAGKVAKDWNTDATKGIPFGSVVTLVVRKGNPKHIKDWDDLLRPGVEVIT
PSPLSSGSAKWNLLAPYAVKSEGGAHGDAGVDFIRKLVTEHVKLRPGSGREATDVFVQGSGDVLISYENEAIATERAGKP
VEHLNLAQTFKIDNPVAVVNTSPHLQAAVAFKNFQYTAAAQKVWAQAGFRPVDPAVAADFRDQYPVPAKLWTIADLGGWS
AADPQLFDKNTGSITKIYTQATG

Sequences:

>Translated_343_residues
MDIRTAARWRPVLALVLTAGVVAGCHGGASDAVGGTGPADARTSITLVAYSVPEPGWSKIIPAFNASDEGKGIQVVTSYG
ASGDQSRGVVDGKPADVVNFSVEPDIARLVKAGKVAKDWNTDATKGIPFGSVVTLVVRKGNPKHIKDWDDLLRPGVEVIT
PSPLSSGSAKWNLLAPYAVKSEGGAHGDAGVDFIRKLVTEHVKLRPGSGREATDVFVQGSGDVLISYENEAIATERAGKP
VEHLNLAQTFKIDNPVAVVNTSPHLQAAVAFKNFQYTAAAQKVWAQAGFRPVDPAVAADFRDQYPVPAKLWTIADLGGWS
AADPQLFDKNTGSITKIYTQATG
>Mature_343_residues
MDIRTAARWRPVLALVLTAGVVAGCHGGASDAVGGTGPADARTSITLVAYSVPEPGWSKIIPAFNASDEGKGIQVVTSYG
ASGDQSRGVVDGKPADVVNFSVEPDIARLVKAGKVAKDWNTDATKGIPFGSVVTLVVRKGNPKHIKDWDDLLRPGVEVIT
PSPLSSGSAKWNLLAPYAVKSEGGAHGDAGVDFIRKLVTEHVKLRPGSGREATDVFVQGSGDVLISYENEAIATERAGKP
VEHLNLAQTFKIDNPVAVVNTSPHLQAAVAFKNFQYTAAAQKVWAQAGFRPVDPAVAADFRDQYPVPAKLWTIADLGGWS
AADPQLFDKNTGSITKIYTQATG

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=304, Percent_Identity=33.5526315789474, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788765, Length=293, Percent_Identity=30.7167235494881, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR006311
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36119; Mature: 36119

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRTAARWRPVLALVLTAGVVAGCHGGASDAVGGTGPADARTSITLVAYSVPEPGWSKI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHC
IPAFNASDEGKGIQVVTSYGASGDQSRGVVDGKPADVVNFSVEPDIARLVKAGKVAKDWN
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCC
TDATKGIPFGSVVTLVVRKGNPKHIKDWDDLLRPGVEVITPSPLSSGSAKWNLLAPYAVK
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCEEEECCEEEC
SEGGAHGDAGVDFIRKLVTEHVKLRPGSGREATDVFVQGSGDVLISYENEAIATERAGKP
CCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCEEEEECCCEEEHHHCCCC
VEHLNLAQTFKIDNPVAVVNTSPHLQAAVAFKNFQYTAAAQKVWAQAGFRPVDPAVAADF
HHHCCCHHEEECCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHCC
RDQYPVPAKLWTIADLGGWSAADPQLFDKNTGSITKIYTQATG
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MDIRTAARWRPVLALVLTAGVVAGCHGGASDAVGGTGPADARTSITLVAYSVPEPGWSKI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHC
IPAFNASDEGKGIQVVTSYGASGDQSRGVVDGKPADVVNFSVEPDIARLVKAGKVAKDWN
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCC
TDATKGIPFGSVVTLVVRKGNPKHIKDWDDLLRPGVEVITPSPLSSGSAKWNLLAPYAVK
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCEEEECCEEEC
SEGGAHGDAGVDFIRKLVTEHVKLRPGSGREATDVFVQGSGDVLISYENEAIATERAGKP
CCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCEEEEECCCEEEHHHCCCC
VEHLNLAQTFKIDNPVAVVNTSPHLQAAVAFKNFQYTAAAQKVWAQAGFRPVDPAVAADF
HHHCCCHHEEECCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHCC
RDQYPVPAKLWTIADLGGWSAADPQLFDKNTGSITKIYTQATG
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]