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The map label for this gene is cysT

Identifier: 41408310

GI number: 41408310

Start: 2459266

End: 2460138

Strand: Reverse

Name: cysT

Synonym: MAP2212c

Alternate gene names: 41408310

Gene position: 2460138-2459266 (Counterclockwise)

Preceding gene: 41408311

Following gene: 41408309

Centisome position: 50.94

GC content: 69.87

Gene sequence:

>873_bases
ATGACGGCCATCACTCCCGACCCGCTGGCCATCCGGCCCGAACTCGGCGGCGACCCCGGACACCAGCCGGTGCCCGGACG
CGGCCGCGGCACCACCGCGCTGCGGGTGGGGGTGGCCACGGTGTGGCTGTCGGTGATCGTGCTGCTGCCGCTGGCCGCCA
TCGCCTGGCAGTCCGCCGGCGGCGGATGGCACGTGTTCTGGCTGGCGGTTACGTCGAACGCCGCGCTGGAATCGTTCCGG
GTGACGCTGACGATCTCGGCCGGGGTGACCGTGCTCAACCTGGTCTTCGGCCTGGTGATCGCCTGGGTGCTGGTGCGCGA
CGAGTTCCCCGGCAAGCGGCTGGTCGACGCGATCATCGACCTGCCGTTCGCGCTGCCCACCATCGTGGCCAGCCTGGTGA
TGTTGGCGCTGTACGGCAACAACAGCCCGGTGGGTCTGCACCTGCAGCACACCGCGGCGGGTGTGGCGGTGGCGTTGGCC
TTCGTCACGCTGCCGTTCGTGGTGCGTGCGGTCCAGCCGGTGCTGCTGGAGCTGGATCGCGAAACCGAGGAGGCGGCCGC
GTCGCTGGGCGCCAGCGGCCCGAAGATCTTCACCTCCGTGGTGCTGCCCGCGTTGCTGCCCGCGTTGTTGTCCGGTGCGG
GCCTGGCGTTTTCACGCGCGATCGGCGAGTTCGGCTCGGTGGTGCTGATCGGAGGTGCGGTGCCGGGCAAGACCGAAGTG
TCCTCGCAGTGGATCCGGACCCTGATCGAGAACGACGACCGGACCGGCGCCGCCGCGATATCGATTGTGCTGTTGGCGAT
TTCCTTTGTGGTGCTGCTGATCCTGCGGGTGGTCGGTGGGCGCGCGGCCAAGCGTGAGGAGCTGGCCGCATGA

Upstream 100 bases:

>100_bases
TGTGGACCATCGCCGATCTGGGCGGCTGGAGCGCCGCGGACCCGCAGCTGTTCGACAAGAACACCGGCAGCATCACCAAG
ATCTACACGCAGGCCACCGG

Downstream 100 bases:

>100_bases
CGTCGTCGCCCGGGGTCCGCTATGGCCTGCGATTCGTGGCGCTGGCCTACATTTTCGTGCTGCTCGTCATTCCGGTGAGC
CTGATCCTGTGGCGGACGTT

Product: CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTAITPDPLAIRPELGGDPGHQPVPGRGRGTTALRVGVATVWLSVIVLLPLAAIAWQSAGGGWHVFWLAVTSNAALESFR
VTLTISAGVTVLNLVFGLVIAWVLVRDEFPGKRLVDAIIDLPFALPTIVASLVMLALYGNNSPVGLHLQHTAAGVAVALA
FVTLPFVVRAVQPVLLELDRETEEAAASLGASGPKIFTSVVLPALLPALLSGAGLAFSRAIGEFGSVVLIGGAVPGKTEV
SSQWIRTLIENDDRTGAAAISIVLLAISFVVLLILRVVGGRAAKREELAA

Sequences:

>Translated_290_residues
MTAITPDPLAIRPELGGDPGHQPVPGRGRGTTALRVGVATVWLSVIVLLPLAAIAWQSAGGGWHVFWLAVTSNAALESFR
VTLTISAGVTVLNLVFGLVIAWVLVRDEFPGKRLVDAIIDLPFALPTIVASLVMLALYGNNSPVGLHLQHTAAGVAVALA
FVTLPFVVRAVQPVLLELDRETEEAAASLGASGPKIFTSVVLPALLPALLSGAGLAFSRAIGEFGSVVLIGGAVPGKTEV
SSQWIRTLIENDDRTGAAAISIVLLAISFVVLLILRVVGGRAAKREELAA
>Mature_289_residues
TAITPDPLAIRPELGGDPGHQPVPGRGRGTTALRVGVATVWLSVIVLLPLAAIAWQSAGGGWHVFWLAVTSNAALESFRV
TLTISAGVTVLNLVFGLVIAWVLVRDEFPGKRLVDAIIDLPFALPTIVASLVMLALYGNNSPVGLHLQHTAAGVAVALAF
VTLPFVVRAVQPVLLELDRETEEAAASLGASGPKIFTSVVLPALLPALLSGAGLAFSRAIGEFGSVVLIGGAVPGKTEVS
SQWIRTLIENDDRTGAAAISIVLLAISFVVLLILRVVGGRAAKREELAA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=236, Percent_Identity=42.7966101694915, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI87082099, Length=265, Percent_Identity=33.2075471698113, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1786980, Length=203, Percent_Identity=33.4975369458128, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30085; Mature: 29954

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITPDPLAIRPELGGDPGHQPVPGRGRGTTALRVGVATVWLSVIVLLPLAAIAWQSAG
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGWHVFWLAVTSNAALESFRVTLTISAGVTVLNLVFGLVIAWVLVRDEFPGKRLVDAIID
CCEEEEEEEEECCHHHHHEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LPFALPTIVASLVMLALYGNNSPVGLHLQHTAAGVAVALAFVTLPFVVRAVQPVLLELDR
CHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETEEAAASLGASGPKIFTSVVLPALLPALLSGAGLAFSRAIGEFGSVVLIGGAVPGKTEV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCHH
SSQWIRTLIENDDRTGAAAISIVLLAISFVVLLILRVVGGRAAKREELAA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
TAITPDPLAIRPELGGDPGHQPVPGRGRGTTALRVGVATVWLSVIVLLPLAAIAWQSAG
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGWHVFWLAVTSNAALESFRVTLTISAGVTVLNLVFGLVIAWVLVRDEFPGKRLVDAIID
CCEEEEEEEEECCHHHHHEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LPFALPTIVASLVMLALYGNNSPVGLHLQHTAAGVAVALAFVTLPFVVRAVQPVLLELDR
CHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETEEAAASLGASGPKIFTSVVLPALLPALLSGAGLAFSRAIGEFGSVVLIGGAVPGKTEV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCHH
SSQWIRTLIENDDRTGAAAISIVLLAISFVVLLILRVVGGRAAKREELAA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]