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The map label for this gene is trpE2

Identifier: 41408303

GI number: 41408303

Start: 2450285

End: 2451637

Strand: Reverse

Name: trpE2

Synonym: MAP2205c

Alternate gene names: 41408303

Gene position: 2451637-2450285 (Counterclockwise)

Preceding gene: 41408305

Following gene: 41408302

Centisome position: 50.76

GC content: 69.4

Gene sequence:

>1353_bases
GTGACCGAGGTCAGCGTCGAGACAACTTCCGCCGGCTCTGAGTCGCCGTCGATTCCTCTTCCGGTGCACATCGACCCGGC
GGACCTGGCCGCCGAGCTGGCCGTGGTGCTCTCCGAGCGTGCCGGGGAAGAGTATTTGCTCTACGAGCGCGGCGGTGAAT
GGGTGCTGGCCACCGGGGTGCGCGCCATGATCGAGCTGGACAGCGACGAGCTGCGGGTGATCCGCGACGGCGTGACCCAG
CGCCAACACTGGTCGGGCCGGCCGGGGCCGGTGCTCGGTGAGGCCATCGATCGGCTGCTGCTGGAGACCGACCAGCTTTT
CGGCTGGATCGCCTTCGAGTTCGGCGTCTATCGCTACGGGCTGCAGCAGCGACTGGCGCCGGGCACCGCGCTGGCCCGGG
TGTTCTGGCCGAACGGACGCATCGTGGTGACCCGCGAGGCGATCCAGCTGTTCGGGACGTCGACCGGCCGGCGCGACGAC
GTGCTGGGCGTGCTCGGCGACGGGGTGCCGGGGCTGCGCGACGCCTCGGCGGTCGACGTCGTCACCGACCCATCGAACTA
TCGCGATCGGGTGGCCTCGGCCGTCGCGGAGATCGCTGCCGGCCGCTATCACAAGGTGATTCTTTCCCGTTGTCTCCAAG
TGCCTTTCGCGGTGGACTTCCCCTCCACCTACCGGCTGGCCCGTCGGCACAACACCCCGGTGCGGTCGTTTCTGCTGCGG
CTTGGCGGGATTCGCGCGGTGGGCTACAGCCCGGAACTGGTTGCGGCCGTGCGTCACGACGGCGTCGTGGTGACCGAACC
GCTGGCGGGCACCCGCGCGTTCGGCCGCGGCGCCCTGCACGACCGCCAGGCCCGTGACGACCTGGAGTCGAACTCCAAAG
AGATTGTCGAGCACGCGATTTCGGTGCGAAGCTCGCTGCAGGAGATGGCCGAGATCGCCGAGCCCGGCACCGCCGTGGTC
ACCGACTTCATGACGGTCCGGGAACGGGGCAGCGTCCAGCATCTCGGCTCCACGGTCAGCGGGCGGCTGGGCACGTCCAA
CGACCGGATGGACGCGCTGGAAGCGCTGTTCCCCGCCGTCACCGCCTCGGGCATCCCGAAAGCGGGCGGAGTGGAGGCGA
TCCTGCGTCTCGACGAGGGACCACGCGGGCTGTATTCGGGTGCGGTGGTGATGGTTTCGGCCGACGGCGCGCTGGACGCG
GCGTTGACGTTGCGCGCGGCCTACGAGCATGACGGCAAGACCTGGCTGCGGGCCGGCGCCGGCATCATCGAAGAGTCGAC
GCCCGAGCGCGAATTCGAGGAGACCTGCGAGAAGCTGTCCACCCTCGCGCCGTATCTCATTGCGCGCCAATAG

Upstream 100 bases:

>100_bases
CGGCGGGAAAACGTTGCGACGACGATCACCGCCCTTCTGGGAATCCGACTGACGAGATCGGTTGCGCAAAGCTATATAGG
TTAGGCTACATTTACTAACC

Downstream 100 bases:

>100_bases
CTTCCCGGGGCTGCCGCTGCCTGGCGTGTGCCGGGTCACGGTTCGGCGCTAAGGGACTTTCGGCCCTAGGTGGTCGCCTC
CGCCGCGTGTCATCTTCATG

Product: salicylate synthase MbtI

Products: NA

Alternate protein names: Mycobactin synthase protein I; Salicylate synthase mbtI

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTEVSVETTSAGSESPSIPLPVHIDPADLAAELAVVLSERAGEEYLLYERGGEWVLATGVRAMIELDSDELRVIRDGVTQ
RQHWSGRPGPVLGEAIDRLLLETDQLFGWIAFEFGVYRYGLQQRLAPGTALARVFWPNGRIVVTREAIQLFGTSTGRRDD
VLGVLGDGVPGLRDASAVDVVTDPSNYRDRVASAVAEIAAGRYHKVILSRCLQVPFAVDFPSTYRLARRHNTPVRSFLLR
LGGIRAVGYSPELVAAVRHDGVVVTEPLAGTRAFGRGALHDRQARDDLESNSKEIVEHAISVRSSLQEMAEIAEPGTAVV
TDFMTVRERGSVQHLGSTVSGRLGTSNDRMDALEALFPAVTASGIPKAGGVEAILRLDEGPRGLYSGAVVMVSADGALDA
ALTLRAAYEHDGKTWLRAGAGIIEESTPEREFEETCEKLSTLAPYLIARQ

Sequences:

>Translated_450_residues
MTEVSVETTSAGSESPSIPLPVHIDPADLAAELAVVLSERAGEEYLLYERGGEWVLATGVRAMIELDSDELRVIRDGVTQ
RQHWSGRPGPVLGEAIDRLLLETDQLFGWIAFEFGVYRYGLQQRLAPGTALARVFWPNGRIVVTREAIQLFGTSTGRRDD
VLGVLGDGVPGLRDASAVDVVTDPSNYRDRVASAVAEIAAGRYHKVILSRCLQVPFAVDFPSTYRLARRHNTPVRSFLLR
LGGIRAVGYSPELVAAVRHDGVVVTEPLAGTRAFGRGALHDRQARDDLESNSKEIVEHAISVRSSLQEMAEIAEPGTAVV
TDFMTVRERGSVQHLGSTVSGRLGTSNDRMDALEALFPAVTASGIPKAGGVEAILRLDEGPRGLYSGAVVMVSADGALDA
ALTLRAAYEHDGKTWLRAGAGIIEESTPEREFEETCEKLSTLAPYLIARQ
>Mature_449_residues
TEVSVETTSAGSESPSIPLPVHIDPADLAAELAVVLSERAGEEYLLYERGGEWVLATGVRAMIELDSDELRVIRDGVTQR
QHWSGRPGPVLGEAIDRLLLETDQLFGWIAFEFGVYRYGLQQRLAPGTALARVFWPNGRIVVTREAIQLFGTSTGRRDDV
LGVLGDGVPGLRDASAVDVVTDPSNYRDRVASAVAEIAAGRYHKVILSRCLQVPFAVDFPSTYRLARRHNTPVRSFLLRL
GGIRAVGYSPELVAAVRHDGVVVTEPLAGTRAFGRGALHDRQARDDLESNSKEIVEHAISVRSSLQEMAEIAEPGTAVVT
DFMTVRERGSVQHLGSTVSGRLGTSNDRMDALEALFPAVTASGIPKAGGVEAILRLDEGPRGLYSGAVVMVSADGALDAA
LTLRAAYEHDGKTWLRAGAGIIEESTPEREFEETCEKLSTLAPYLIARQ

Specific function: Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicyl

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family. Salicylate synthase subfamily

Homologues:

Organism=Escherichia coli, GI1787518, Length=250, Percent_Identity=32, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788114, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786809, Length=272, Percent_Identity=27.5735294117647, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082077, Length=267, Percent_Identity=28.4644194756554, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320935, Length=254, Percent_Identity=32.2834645669291, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): MBTI_MYCPA (Q73XV3)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_961139.1
- HSSP:   Q9X9I8
- ProteinModelPortal:   Q73XV3
- SMR:   Q73XV3
- EnsemblBacteria:   EBMYCT00000039365
- GeneID:   2718699
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2205c
- NMPDR:   fig|262316.1.peg.2205
- GeneTree:   EBGT00050000015088
- HOGENOM:   HBG326929
- OMA:   TPEREFE
- ProtClustDB:   PRK07912
- BRENDA:   5.4.4.2
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR015890
- InterPro:   IPR019996
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR03494

Pfam domain/function: PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =5.4.4.2

Molecular weight: Translated: 48636; Mature: 48504

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: BINDING 271-271 BINDING 334-334 BINDING 385-385 BINDING 405-405 BINDING 438-438

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVSVETTSAGSESPSIPLPVHIDPADLAAELAVVLSERAGEEYLLYERGGEWVLATGV
CCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCC
RAMIELDSDELRVIRDGVTQRQHWSGRPGPVLGEAIDRLLLETDQLFGWIAFEFGVYRYG
EEEEEECCHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQQRLAPGTALARVFWPNGRIVVTREAIQLFGTSTGRRDDVLGVLGDGVPGLRDASAVDV
HHHHCCCCHHEEEEECCCCCEEEEHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEEEE
VTDPSNYRDRVASAVAEIAAGRYHKVILSRCLQVPFAVDFPSTYRLARRHNTPVRSFLLR
EECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCHHHHHHHH
LGGIRAVGYSPELVAAVRHDGVVVTEPLAGTRAFGRGALHDRQARDDLESNSKEIVEHAI
HCCEEEECCCCHHHHHHHCCCEEEECCCCCCHHHCCCCCCCHHHHHHHHCCHHHHHHHHH
SVRSSLQEMAEIAEPGTAVVTDFMTVRERGSVQHLGSTVSGRLGTSNDRMDALEALFPAV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCHHCCCCCCCCCHHHHHHHHHHHH
TASGIPKAGGVEAILRLDEGPRGLYSGAVVMVSADGALDAALTLRAAYEHDGKTWLRAGA
HHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCC
GIIEESTPEREFEETCEKLSTLAPYLIARQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEVSVETTSAGSESPSIPLPVHIDPADLAAELAVVLSERAGEEYLLYERGGEWVLATGV
CCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCC
RAMIELDSDELRVIRDGVTQRQHWSGRPGPVLGEAIDRLLLETDQLFGWIAFEFGVYRYG
EEEEEECCHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQQRLAPGTALARVFWPNGRIVVTREAIQLFGTSTGRRDDVLGVLGDGVPGLRDASAVDV
HHHHCCCCHHEEEEECCCCCEEEEHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEEEE
VTDPSNYRDRVASAVAEIAAGRYHKVILSRCLQVPFAVDFPSTYRLARRHNTPVRSFLLR
EECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCHHHHHHHH
LGGIRAVGYSPELVAAVRHDGVVVTEPLAGTRAFGRGALHDRQARDDLESNSKEIVEHAI
HCCEEEECCCCHHHHHHHCCCEEEECCCCCCHHHCCCCCCCHHHHHHHHCCHHHHHHHHH
SVRSSLQEMAEIAEPGTAVVTDFMTVRERGSVQHLGSTVSGRLGTSNDRMDALEALFPAV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCHHCCCCCCCCCHHHHHHHHHHHH
TASGIPKAGGVEAILRLDEGPRGLYSGAVVMVSADGALDAALTLRAAYEHDGKTWLRAGA
HHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCC
GIIEESTPEREFEETCEKLSTLAPYLIARQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA