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The map label for this gene is sseB

Identifier: 41408144

GI number: 41408144

Start: 2254614

End: 2255468

Strand: Direct

Name: sseB

Synonym: MAP2046

Alternate gene names: 41408144

Gene position: 2254614-2255468 (Clockwise)

Preceding gene: 41408143

Following gene: 41408145

Centisome position: 46.68

GC content: 73.33

Gene sequence:

>855_bases
GTGGGGGCACGCGATCAGGTATTGATCACCGCGACCGAGTTGGCCGACGTCATCGAGGCCGGAGACCCGGTGAGCATCCT
GGACGTGCGGTGGAGGCTCGACGAGCCCGACGGCCGCGCGGCCTACCTGCAGGGCCACCTTCCCGACGCGGTGTACGTCT
CGCTCGAGGACGAGCTCAGCGACCACACGGTGTCCGGGCGGGGCCGTCACCCGCTTCCGTCGGGACCCAGCCTGCAGGCG
GCCGCCCGCCGATGGGGCATCCGGCAGGACACACCGGTGGTGGTCTACGACGACTGGAACCGCGCCGGCTCCGCGCGCGC
CTGGTGGCTGCTGCGCGCGGCCGGGCTGGACAACGTCCGCATTCTGGACGGCGGGCTGGCCGCCTGGCGGGCGACCGGCG
GCCGACTCGTGTCCGGGCCCGTCGAACCAGTGCCCGGGAATGTGACTGTGCCGCATGGTGATTTGCATTCCGGCAACCGT
CCGACCGTGACGACCGAGCAGGTGGCCGCGGGCGCCGCCACACTCATCGACGCCCGGGCGCCGGAACGCTACCGCGGCGA
AATGGAACCACTCGATCCCGTCGCCGGGCACATTCCCGGCGCCGAGAATCTGCCCAGCGGCGAGGTTTTGGCGGCCGACG
GCACCTTCCTCGGCGACGACGCGCTCGCCCGGGTGTTCGCCGAGCACCGAATCGAGCGGCACGGCGCGGTGGCCGCCTAT
TGCGGCTCGGGCGTCACCGCCACGGTGACGATCGCCGCGCTCGCGGCGGTGGGCCGAACGGCGGCGCTGTACCCGGGATC
GTGGTCGGAGTGGTGCGCGGACCCGGCCCGCCCGGTGGAACGCGGCGGCGCGTAG

Upstream 100 bases:

>100_bases
CGGCCCCCGTCACCCGGGCCTTTTCGCCGCGCCGCTCGGGCGGGCGCTGGTTTGCCCGACCGCCCTGGTTAGTCTCGCTA
CAGACGACAAGGAGGATCCG

Downstream 100 bases:

>100_bases
GCGGCTGCACCACCTGTTAGCCTGTTCCCGACAGCGCCTAGGGTTCCGGCACCACGCGGTGTGACTGGTCCGAGCGGCGC
CACCGCCTTCCCCGACGGAA

Product: SseB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MGARDQVLITATELADVIEAGDPVSILDVRWRLDEPDGRAAYLQGHLPDAVYVSLEDELSDHTVSGRGRHPLPSGPSLQA
AARRWGIRQDTPVVVYDDWNRAGSARAWWLLRAAGLDNVRILDGGLAAWRATGGRLVSGPVEPVPGNVTVPHGDLHSGNR
PTVTTEQVAAGAATLIDARAPERYRGEMEPLDPVAGHIPGAENLPSGEVLAADGTFLGDDALARVFAEHRIERHGAVAAY
CGSGVTATVTIAALAAVGRTAALYPGSWSEWCADPARPVERGGA

Sequences:

>Translated_284_residues
MGARDQVLITATELADVIEAGDPVSILDVRWRLDEPDGRAAYLQGHLPDAVYVSLEDELSDHTVSGRGRHPLPSGPSLQA
AARRWGIRQDTPVVVYDDWNRAGSARAWWLLRAAGLDNVRILDGGLAAWRATGGRLVSGPVEPVPGNVTVPHGDLHSGNR
PTVTTEQVAAGAATLIDARAPERYRGEMEPLDPVAGHIPGAENLPSGEVLAADGTFLGDDALARVFAEHRIERHGAVAAY
CGSGVTATVTIAALAAVGRTAALYPGSWSEWCADPARPVERGGA
>Mature_283_residues
GARDQVLITATELADVIEAGDPVSILDVRWRLDEPDGRAAYLQGHLPDAVYVSLEDELSDHTVSGRGRHPLPSGPSLQAA
ARRWGIRQDTPVVVYDDWNRAGSARAWWLLRAAGLDNVRILDGGLAAWRATGGRLVSGPVEPVPGNVTVPHGDLHSGNRP
TVTTEQVAAGAATLIDARAPERYRGEMEPLDPVAGHIPGAENLPSGEVLAADGTFLGDDALARVFAEHRIERHGAVAAYC
GSGVTATVTIAALAAVGRTAALYPGSWSEWCADPARPVERGGA

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=276, Percent_Identity=31.5217391304348, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI61835204, Length=276, Percent_Identity=31.5217391304348, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI194473681, Length=276, Percent_Identity=31.5217391304348, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI17402865, Length=294, Percent_Identity=30.6122448979592, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI87082121, Length=248, Percent_Identity=37.9032258064516, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI87081967, Length=258, Percent_Identity=25.968992248062, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71997283, Length=252, Percent_Identity=30.5555555555556, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17561888, Length=247, Percent_Identity=27.1255060728745, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115534702, Length=280, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 29915; Mature: 29783

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGARDQVLITATELADVIEAGDPVSILDVRWRLDEPDGRAAYLQGHLPDAVYVSLEDELS
CCCCCCEEEEHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEECCCCCEEEEEECCCCC
DHTVSGRGRHPLPSGPSLQAAARRWGIRQDTPVVVYDDWNRAGSARAWWLLRAAGLDNVR
CCEECCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCEE
ILDGGLAAWRATGGRLVSGPVEPVPGNVTVPHGDLHSGNRPTVTTEQVAAGAATLIDARA
EEECCCEEECCCCCEEECCCCCCCCCCEECCCCCCCCCCCCCEEHHHHHCCHHHEEECCC
PERYRGEMEPLDPVAGHIPGAENLPSGEVLAADGTFLGDDALARVFAEHRIERHGAVAAY
CHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCEECHHHHHHHHHHHHHHHCCCEEEE
CGSGVTATVTIAALAAVGRTAALYPGSWSEWCADPARPVERGGA
CCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCHHHCCCC
>Mature Secondary Structure 
GARDQVLITATELADVIEAGDPVSILDVRWRLDEPDGRAAYLQGHLPDAVYVSLEDELS
CCCCCEEEEHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEECCCCCEEEEEECCCCC
DHTVSGRGRHPLPSGPSLQAAARRWGIRQDTPVVVYDDWNRAGSARAWWLLRAAGLDNVR
CCEECCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCEE
ILDGGLAAWRATGGRLVSGPVEPVPGNVTVPHGDLHSGNRPTVTTEQVAAGAATLIDARA
EEECCCEEECCCCCEEECCCCCCCCCCEECCCCCCCCCCCCCEEHHHHHCCHHHEEECCC
PERYRGEMEPLDPVAGHIPGAENLPSGEVLAADGTFLGDDALARVFAEHRIERHGAVAAY
CHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCEECHHHHHHHHHHHHHHHCCCEEEE
CGSGVTATVTIAALAAVGRTAALYPGSWSEWCADPARPVERGGA
CCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]