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The map label for this gene is nirA_1

Identifier: 41408133

GI number: 41408133

Start: 2243311

End: 2244978

Strand: Direct

Name: nirA_1

Synonym: MAP2035

Alternate gene names: 41408133

Gene position: 2243311-2244978 (Clockwise)

Preceding gene: 41408131

Following gene: 41408134

Centisome position: 46.45

GC content: 66.91

Gene sequence:

>1668_bases
ATGACCACCGCACGTCCCGTCAAGACCCGCAACGAGGGTCAGTGGGCGCTGGGAGATCGCGAACCGCTCAACGACACCGA
GAAGATCAAGCTGGCCGACGGGCCGCTGAACGTGCGCGAGCGCATCATCAACGTCTACGCCAAGCAGGGTTTCGACAGCA
TCGACAAGTCCGACCTGCGCGGCCGCTTCCGGTGGATGGGCCTGTACACCCAACGCGAGCAGGGCTACGACGGCAGCTGG
ACCGGTGACGACAACACCGACAAGATCGAAGCCAAGTACTTCATGATGCGGGTCCGCTCCGACGGCAAGGCGATGTCGGC
GCACACCATGCGCACGCTCGGCCAGATCTCCACCGAATTCGCCCGCGACACCGCCGATATCAGCGACCGGGAAAACCTGC
AGCTGCACTGGATTCGCATCGAGGACGTGCCCGAGATCTGGCGACGGCTCGAGTCCGTCGGGCTGCAGACCACCGAGGCC
TGCGGCGACTGCCCGCGCGGCATCCACGGCTCGCCGCTGGCCGGCGACTCGCTCGACGAGGTGCTCGATCCGTCGCCCGC
GATCGAGGAGATCGTCCGTCGCTCCCTGAACAACCCCGAATACGCCAACCTGCCGCGCAAGTACAAAACCGCGGTCTCGG
GCCTGCAGGACGTCTCGCACGAGACCCACGACGTCGCGTTCGTCGGCGTCGAGCACCCCGAGCACGGACCCGGCCTGGAC
CTGTGGGTGGGCGGCGGCCTGTCCACCAACCCGATGCTGGCCCAGCGGCTCAGCGTGTGGGTTCCGCTGGACGAGGTGCC
CGACGTGTGGGAAGCCGTCACCCAGCTGTTCCGCGACTACGGCTACCGGCGGCTGCGCGCCAAGGCCCGGCTGAAGTTCC
TGGTCAAGGACTGGGGCATAGAAAAGTTCCGTGAAATTCTCGAACAGGAATACCTCAACCGGCGGCTGATCGATGGACCG
GCGCCCGCGCCGGTCAAGCACACCATCGACCACGTCGGGGTGCAGAAGATCAAGAACGGGCTCAACGCCGTCGGCGTCGC
GCCCATCGCCGGGCGCGTCTCGGGCACCACCCTGTCCGCGGTGGCCGACCTGATGGAACAGGTCGGCTCCGATCGGGCGC
GCTGGACGCCGTTTCAGAAGCTGGTCATCCTCGACGTGCCCGACGACAAGGTCGACGAACTCGTCACTGGCCTGGACGCG
CTGGGCCTGCCGTCGCGGCCGTCGTCGTGGCGCAAGAACACCATGGCCTGCACCGGGATCGAGTTCTGCAAGCTGTCGTT
CGCCGAAACCCGGGTGCGCACCCAGACTTTGGTGCCCGAGCTGGAGCGGCGCCTGGCCGACGTCGACGCCCAGCTCGACG
CGCCGATCAGCGTCCACCTCAACGGATGTCCGAACTCGTGCGCCCGAATCCAGGTGGCCGACATCGGCTTCAAGGGCCAA
TGGATCGACAACGGCGACGGCACATCGGTCGAGGGCTTCCAGGTTCACCTGGGCGGCGGCCTGGGCGAGCAGAGCGGCTT
CGGCCGAAAACTGCGGCAGCACAAGGTCACCAGCGAAGAACTGGGCGACTACATCGACCGGGTGACGCGCAAGTACCTCG
AGGGCCGCAACGACGGTGAGACGTTCGCGTCCTGGGCGTTGCGCGCCGACGAGGAAGAGCTGCGCTGA

Upstream 100 bases:

>100_bases
ACACAGCAACCACATGAAAGCCATACCCGACGTCCCTGGGCAATTCGAAGCCTAAGGCCGACGGCCCCGAACACCCTTCA
AGAAAGGTAAGGAACACCAC

Downstream 100 bases:

>100_bases
GGTCCGAGGTCGAAATGAGCGACGTGGCAACCGACTTCACCGAGGAACAGCTGCGCGAGCTGGCCGAGCGCGGCGCCTCG
GAGCTGGAGGGCGCCAGCGC

Product: NirA_1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTTARPVKTRNEGQWALGDREPLNDTEKIKLADGPLNVRERIINVYAKQGFDSIDKSDLRGRFRWMGLYTQREQGYDGSW
TGDDNTDKIEAKYFMMRVRSDGKAMSAHTMRTLGQISTEFARDTADISDRENLQLHWIRIEDVPEIWRRLESVGLQTTEA
CGDCPRGIHGSPLAGDSLDEVLDPSPAIEEIVRRSLNNPEYANLPRKYKTAVSGLQDVSHETHDVAFVGVEHPEHGPGLD
LWVGGGLSTNPMLAQRLSVWVPLDEVPDVWEAVTQLFRDYGYRRLRAKARLKFLVKDWGIEKFREILEQEYLNRRLIDGP
APAPVKHTIDHVGVQKIKNGLNAVGVAPIAGRVSGTTLSAVADLMEQVGSDRARWTPFQKLVILDVPDDKVDELVTGLDA
LGLPSRPSSWRKNTMACTGIEFCKLSFAETRVRTQTLVPELERRLADVDAQLDAPISVHLNGCPNSCARIQVADIGFKGQ
WIDNGDGTSVEGFQVHLGGGLGEQSGFGRKLRQHKVTSEELGDYIDRVTRKYLEGRNDGETFASWALRADEEELR

Sequences:

>Translated_555_residues
MTTARPVKTRNEGQWALGDREPLNDTEKIKLADGPLNVRERIINVYAKQGFDSIDKSDLRGRFRWMGLYTQREQGYDGSW
TGDDNTDKIEAKYFMMRVRSDGKAMSAHTMRTLGQISTEFARDTADISDRENLQLHWIRIEDVPEIWRRLESVGLQTTEA
CGDCPRGIHGSPLAGDSLDEVLDPSPAIEEIVRRSLNNPEYANLPRKYKTAVSGLQDVSHETHDVAFVGVEHPEHGPGLD
LWVGGGLSTNPMLAQRLSVWVPLDEVPDVWEAVTQLFRDYGYRRLRAKARLKFLVKDWGIEKFREILEQEYLNRRLIDGP
APAPVKHTIDHVGVQKIKNGLNAVGVAPIAGRVSGTTLSAVADLMEQVGSDRARWTPFQKLVILDVPDDKVDELVTGLDA
LGLPSRPSSWRKNTMACTGIEFCKLSFAETRVRTQTLVPELERRLADVDAQLDAPISVHLNGCPNSCARIQVADIGFKGQ
WIDNGDGTSVEGFQVHLGGGLGEQSGFGRKLRQHKVTSEELGDYIDRVTRKYLEGRNDGETFASWALRADEEELR
>Mature_554_residues
TTARPVKTRNEGQWALGDREPLNDTEKIKLADGPLNVRERIINVYAKQGFDSIDKSDLRGRFRWMGLYTQREQGYDGSWT
GDDNTDKIEAKYFMMRVRSDGKAMSAHTMRTLGQISTEFARDTADISDRENLQLHWIRIEDVPEIWRRLESVGLQTTEAC
GDCPRGIHGSPLAGDSLDEVLDPSPAIEEIVRRSLNNPEYANLPRKYKTAVSGLQDVSHETHDVAFVGVEHPEHGPGLDL
WVGGGLSTNPMLAQRLSVWVPLDEVPDVWEAVTQLFRDYGYRRLRAKARLKFLVKDWGIEKFREILEQEYLNRRLIDGPA
PAPVKHTIDHVGVQKIKNGLNAVGVAPIAGRVSGTTLSAVADLMEQVGSDRARWTPFQKLVILDVPDDKVDELVTGLDAL
GLPSRPSSWRKNTMACTGIEFCKLSFAETRVRTQTLVPELERRLADVDAQLDAPISVHLNGCPNSCARIQVADIGFKGQW
IDNGDGTSVEGFQVHLGGGLGEQSGFGRKLRQHKVTSEELGDYIDRVTRKYLEGRNDGETFASWALRADEEELR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=532, Percent_Identity=24.0601503759398, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322597, Length=553, Percent_Identity=26.5822784810127, Blast_Score=138, Evalue=2e-33,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SIR1_MYCPA (Q73YC1)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_960969.1
- ProteinModelPortal:   Q73YC1
- SMR:   Q73YC1
- EnsemblBacteria:   EBMYCT00000040300
- GeneID:   2719754
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2035
- NMPDR:   fig|262316.1.peg.2035
- GeneTree:   EBGT00050000015728
- HOGENOM:   HBG578916
- OMA:   GMHVPVG
- ProtClustDB:   CLSK872057
- BRENDA:   1.8.7.1
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.7.1

Molecular weight: Translated: 62150; Mature: 62019

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTARPVKTRNEGQWALGDREPLNDTEKIKLADGPLNVRERIINVYAKQGFDSIDKSDLR
CCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHC
GRFRWMGLYTQREQGYDGSWTGDDNTDKIEAKYFMMRVRSDGKAMSAHTMRTLGQISTEF
CEEEEEEEEEHHCCCCCCCCCCCCCCCHHHHHHHEEHECCCCCHHHHHHHHHHHHHHHHH
ARDTADISDRENLQLHWIRIEDVPEIWRRLESVGLQTTEACGDCPRGIHGSPLAGDSLDE
HHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCHHH
VLDPSPAIEEIVRRSLNNPEYANLPRKYKTAVSGLQDVSHETHDVAFVGVEHPEHGPGLD
HCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCE
LWVGGGLSTNPMLAQRLSVWVPLDEVPDVWEAVTQLFRDYGYRRLRAKARLKFLVKDWGI
EEECCCCCCCHHHHHHHHEECCCHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCH
EKFREILEQEYLNRRLIDGPAPAPVKHTIDHVGVQKIKNGLNAVGVAPIAGRVSGTTLSA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
VADLMEQVGSDRARWTPFQKLVILDVPDDKVDELVTGLDALGLPSRPSSWRKNTMACTGI
HHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCH
EFCKLSFAETRVRTQTLVPELERRLADVDAQLDAPISVHLNGCPNSCARIQVADIGFKGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCC
WIDNGDGTSVEGFQVHLGGGLGEQSGFGRKLRQHKVTSEELGDYIDRVTRKYLEGRNDGE
EECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCH
TFASWALRADEEELR
HHHHHHHCCCHHHCC
>Mature Secondary Structure 
TTARPVKTRNEGQWALGDREPLNDTEKIKLADGPLNVRERIINVYAKQGFDSIDKSDLR
CCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHC
GRFRWMGLYTQREQGYDGSWTGDDNTDKIEAKYFMMRVRSDGKAMSAHTMRTLGQISTEF
CEEEEEEEEEHHCCCCCCCCCCCCCCCHHHHHHHEEHECCCCCHHHHHHHHHHHHHHHHH
ARDTADISDRENLQLHWIRIEDVPEIWRRLESVGLQTTEACGDCPRGIHGSPLAGDSLDE
HHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCHHH
VLDPSPAIEEIVRRSLNNPEYANLPRKYKTAVSGLQDVSHETHDVAFVGVEHPEHGPGLD
HCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCE
LWVGGGLSTNPMLAQRLSVWVPLDEVPDVWEAVTQLFRDYGYRRLRAKARLKFLVKDWGI
EEECCCCCCCHHHHHHHHEECCCHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCH
EKFREILEQEYLNRRLIDGPAPAPVKHTIDHVGVQKIKNGLNAVGVAPIAGRVSGTTLSA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
VADLMEQVGSDRARWTPFQKLVILDVPDDKVDELVTGLDALGLPSRPSSWRKNTMACTGI
HHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCH
EFCKLSFAETRVRTQTLVPELERRLADVDAQLDAPISVHLNGCPNSCARIQVADIGFKGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCC
WIDNGDGTSVEGFQVHLGGGLGEQSGFGRKLRQHKVTSEELGDYIDRVTRKYLEGRNDGE
EECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCH
TFASWALRADEEELR
HHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA