Click here to switch to the map view.

The map label for this gene is dagK [H]

Identifier: 41408103

GI number: 41408103

Start: 2213465

End: 2215045

Strand: Direct

Name: dagK [H]

Synonym: MAP2005

Alternate gene names: 41408103

Gene position: 2213465-2215045 (Clockwise)

Preceding gene: 41408102

Following gene: 41408106

Centisome position: 45.83

GC content: 69.64

Gene sequence:

>1581_bases
GTGACGTCCCCGGCTGCACGGCTGCGCCGGTACGAGATCGGCAAGGTGATCGCGCTGACCAACCCGGTCTCCGGGCACGG
CGCCGCGGTGGCCGCCGCGCAGCGGGCGATCGCCCGGCTGCACCGGCGCGGGGTGGAGGTCGTCGAAATCATCGGCGACG
ACGCGCAAGACGCCCGGCACCTGGTCGGCGCGGCACTGGACAAGGGTGCCGACGCGGTCATGGTGACCGGGGGCGACGGG
GTGTTCTCCAACGCGCTACAAGTGTTGGCGGGCACCGACATTCCCGCCGGCATCGTCCCGGCCGGCACCGGCAACGACCA
CGCCCGTGAATTCGGGATCCCCACCAAGGATCCCGAGGCCGCCGCCGACATCATCGTCGACGGCTGGGCCGAAACCGTTG
ACCTGGGCCGGATTCGGGCCGACAACGGATTCGACAAGTGGTTCGGCACCGTGGCGGCCACCGGCTTCGACTCGCTGGTC
ACCGATCGCGCCAACCGGATGCGCTGGCCGCACGGCCGGCTGCGCTATTACGTGGCCATGCTCGCCGAGCTGTCCCAGTT
GCGGCTCTTACCTTTTCGCCTGGTGCTCGACGGCGCGCAGGAGATCGACGCCGAGATCACACTGGCCGCCTTCGGCAACA
CCCGCAGCTACGGCGGCGGAATGCGGATCTGCCCGGCCGCCGACCACGCCGACGGCCTGCTCGACATCACCATGGTGCAC
GAGGCATCGCGCGCCAAGCTGGTCCGCCTGTTCCCCACCGTAATGACGGGCACGCACGTCGAACTCGAGCAGGTGAGCAC
GGTGCGGGCCAAATCCATCCACGTCGAGTGTCCGGGGATCAACGTCTACGCCGACGGCGACTTCGCCTGCCCGCTGCCGG
CGGAGATCTCCGCCGGCAGCGGGCGCGCTGCGGATCCTGCGGGCCGGTCCCTAACCCACGCCCCGGCTCAGCCAGGTCAC
CTCGCCGGCGTCGCCGCCGTCGCGGAATGCCTCGAGCGCCTCGTCCCAGGCCGTCCCCAGCACGGAATCCAGCTCGGCGG
CCAGCGTGTCGGCGCCCTGGGCCATCATCGCGCGCAGCCGCATCTCCCCGACCATGATGTCGCCGTTGGCGCTCATCGCC
CCGCTCCACAGACCCAGTTGCGGGGTGTGGCTGAAGCGGTGGCCGTCGACTCCGGGGCTGGGGTCCTCGGTGACCTCGAA
CCGCAGCACGGACCAGGAGCGCAACGCGTTGGCCAGCTTGGCGCCGGTGCCCACCGGACCGACCCAGTTGGTGACCGCGC
GCAGCTGTCCGGGCATCGCCGGCTGGGGCGTCCAGGTCAGGTTGGCCTTGGCCCCCAGGGTCGACGACAACGCCCACTCG
ACATGCGGGCACACCGCCGCCGGCGAGGCGTGCACGTACACCACGCCAGTCGTCACGTCGGCGAATTGGTTCGACGCTCG
CATTTCCTGCTCCTTCGGTTCCACGAGGGACGTCTTCCCCAACGACCTGTGGACCCGGCAAGCAGGGCAGCTTGCGCAAA
TCTTCGATTTTTTCTGTGTCGCGCGTGTCTATTGTGCCCTGTGATACCCATGTTGCGCTAG

Upstream 100 bases:

>100_bases
GGATGCGCGAGGAGGTCGGCGAGTTGGGAGTGCAGGTGTTACGCGCGGTCAAGGCCACCCTGGACCCGGCCGGAATCCTC
AACCCCGGCAAGCTGATTCC

Downstream 100 bases:

>100_bases
TGTGCGATCCGATTTAGCTGTATTCGGATATCACGGCGTCCGAAATCGCCGGCCACCGGTCCATCGCCCACTCCCCGAAA
TCGCGGTCGGTGAGCACCAC

Product: hypothetical protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTSPAARLRRYEIGKVIALTNPVSGHGAAVAAAQRAIARLHRRGVEVVEIIGDDAQDARHLVGAALDKGADAVMVTGGDG
VFSNALQVLAGTDIPAGIVPAGTGNDHAREFGIPTKDPEAAADIIVDGWAETVDLGRIRADNGFDKWFGTVAATGFDSLV
TDRANRMRWPHGRLRYYVAMLAELSQLRLLPFRLVLDGAQEIDAEITLAAFGNTRSYGGGMRICPAADHADGLLDITMVH
EASRAKLVRLFPTVMTGTHVELEQVSTVRAKSIHVECPGINVYADGDFACPLPAEISAGSGRAADPAGRSLTHAPAQPGH
LAGVAAVAECLERLVPGRPQHGIQLGGQRVGALGHHRAQPHLPDHDVAVGAHRPAPQTQLRGVAEAVAVDSGAGVLGDLE
PQHGPGAQRVGQLGAGAHRTDPVGDRAQLSGHRRLGRPGQVGLGPQGRRQRPLDMRAHRRRRGVHVHHASRHVGELVRRS
HFLLLRFHEGRLPQRPVDPASRAACANLRFFLCRACLLCPVIPMLR

Sequences:

>Translated_526_residues
MTSPAARLRRYEIGKVIALTNPVSGHGAAVAAAQRAIARLHRRGVEVVEIIGDDAQDARHLVGAALDKGADAVMVTGGDG
VFSNALQVLAGTDIPAGIVPAGTGNDHAREFGIPTKDPEAAADIIVDGWAETVDLGRIRADNGFDKWFGTVAATGFDSLV
TDRANRMRWPHGRLRYYVAMLAELSQLRLLPFRLVLDGAQEIDAEITLAAFGNTRSYGGGMRICPAADHADGLLDITMVH
EASRAKLVRLFPTVMTGTHVELEQVSTVRAKSIHVECPGINVYADGDFACPLPAEISAGSGRAADPAGRSLTHAPAQPGH
LAGVAAVAECLERLVPGRPQHGIQLGGQRVGALGHHRAQPHLPDHDVAVGAHRPAPQTQLRGVAEAVAVDSGAGVLGDLE
PQHGPGAQRVGQLGAGAHRTDPVGDRAQLSGHRRLGRPGQVGLGPQGRRQRPLDMRAHRRRRGVHVHHASRHVGELVRRS
HFLLLRFHEGRLPQRPVDPASRAACANLRFFLCRACLLCPVIPMLR
>Mature_525_residues
TSPAARLRRYEIGKVIALTNPVSGHGAAVAAAQRAIARLHRRGVEVVEIIGDDAQDARHLVGAALDKGADAVMVTGGDGV
FSNALQVLAGTDIPAGIVPAGTGNDHAREFGIPTKDPEAAADIIVDGWAETVDLGRIRADNGFDKWFGTVAATGFDSLVT
DRANRMRWPHGRLRYYVAMLAELSQLRLLPFRLVLDGAQEIDAEITLAAFGNTRSYGGGMRICPAADHADGLLDITMVHE
ASRAKLVRLFPTVMTGTHVELEQVSTVRAKSIHVECPGINVYADGDFACPLPAEISAGSGRAADPAGRSLTHAPAQPGHL
AGVAAVAECLERLVPGRPQHGIQLGGQRVGALGHHRAQPHLPDHDVAVGAHRPAPQTQLRGVAEAVAVDSGAGVLGDLEP
QHGPGAQRVGQLGAGAHRTDPVGDRAQLSGHRRLGRPGQVGLGPQGRRQRPLDMRAHRRRRGVHVHHASRHVGELVRRSH
FLLLRFHEGRLPQRPVDPASRAACANLRFFLCRACLLCPVIPMLR

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derive

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Secreted, cell wall (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=209, Percent_Identity=29.6650717703349, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 55960; Mature: 55828

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPAARLRRYEIGKVIALTNPVSGHGAAVAAAQRAIARLHRRGVEVVEIIGDDAQDARH
CCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHH
LVGAALDKGADAVMVTGGDGVFSNALQVLAGTDIPAGIVPAGTGNDHAREFGIPTKDPEA
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHCCCCCCCCHH
AADIIVDGWAETVDLGRIRADNGFDKWFGTVAATGFDSLVTDRANRMRWPHGRLRYYVAM
HHHHEECCCHHHHHHCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHH
LAELSQLRLLPFRLVLDGAQEIDAEITLAAFGNTRSYGGGMRICPAADHADGLLDITMVH
HHHHHHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCEEEEEEH
EASRAKLVRLFPTVMTGTHVELEQVSTVRAKSIHVECPGINVYADGDFACPLPAEISAGS
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHEEEEEEECCCEEEEECCCCCCCCCCCCCCCC
GRAADPAGRSLTHAPAQPGHLAGVAAVAECLERLVPGRPQHGIQLGGQRVGALGHHRAQP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHCCCCCCC
HLPDHDVAVGAHRPAPQTQLRGVAEAVAVDSGAGVLGDLEPQHGPGAQRVGQLGAGAHRT
CCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCC
DPVGDRAQLSGHRRLGRPGQVGLGPQGRRQRPLDMRAHRRRRGVHVHHASRHVGELVRRS
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHCC
HFLLLRFHEGRLPQRPVDPASRAACANLRFFLCRACLLCPVIPMLR
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSPAARLRRYEIGKVIALTNPVSGHGAAVAAAQRAIARLHRRGVEVVEIIGDDAQDARH
CCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHH
LVGAALDKGADAVMVTGGDGVFSNALQVLAGTDIPAGIVPAGTGNDHAREFGIPTKDPEA
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHCCCCCCCCHH
AADIIVDGWAETVDLGRIRADNGFDKWFGTVAATGFDSLVTDRANRMRWPHGRLRYYVAM
HHHHEECCCHHHHHHCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHH
LAELSQLRLLPFRLVLDGAQEIDAEITLAAFGNTRSYGGGMRICPAADHADGLLDITMVH
HHHHHHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCEEEEEEH
EASRAKLVRLFPTVMTGTHVELEQVSTVRAKSIHVECPGINVYADGDFACPLPAEISAGS
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHEEEEEEECCCEEEEECCCCCCCCCCCCCCCC
GRAADPAGRSLTHAPAQPGHLAGVAAVAECLERLVPGRPQHGIQLGGQRVGALGHHRAQP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHCCCCCCC
HLPDHDVAVGAHRPAPQTQLRGVAEAVAVDSGAGVLGDLEPQHGPGAQRVGQLGAGAHRT
CCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCC
DPVGDRAQLSGHRRLGRPGQVGLGPQGRRQRPLDMRAHRRRRGVHVHHASRHVGELVRRS
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHCC
HFLLLRFHEGRLPQRPVDPASRAACANLRFFLCRACLLCPVIPMLR
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]