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The map label for this gene is adhE2

Identifier: 41408106

GI number: 41408106

Start: 2217017

End: 2218102

Strand: Direct

Name: adhE2

Synonym: MAP2008

Alternate gene names: 41408106

Gene position: 2217017-2218102 (Clockwise)

Preceding gene: 41408103

Following gene: 41408107

Centisome position: 45.9

GC content: 69.61

Gene sequence:

>1086_bases
ATGAGTCAGACAGTGCGCGGCGTGATTTCACGCAAGAAGGGCGAACCCGTTGAGTTGGTCGACATCGTCGTTCCCGATCC
CGGGCCGGGTGAGGCGCTGGTCGACGTCATCGCCTGCGGGGTCTGCCACACCGACCTGACCTACCGCGAGGGCGGCATCA
ACGACGAGTACCCGTTTCTGCTCGGCCACGAGGCCGCCGGCCGGGTCGAGGCCGTCGGCCCCGACGTGACCGCGGTGCAG
CCGGGCGACTTCGTGGTGCTGAACTGGCGCGCGGTGTGCGGGCAGTGCCGGGCCTGCAAGCGCGGCCGCCCGCACCTGTG
CTTCGACACCTTCAACGCCACCCAGAAGATGACGCTGACCGACGGCACCGAGCTGACACCGGCGCTGGGCATCGGGGCGT
TCGCCGACAAGACACTGGTGGCCGCCGGCCAGTGCACCAAGGTCGACCCCGCCGCCGATCCCGCCGTCGCCGGGCTGCTG
GGTTGCGGGGTGATGGCCGGGCTGGGCGCGGCGATCAACACCGGTGCCGTCGCCCGCGATGACACCGTCGCGGTGATCGG
CTGCGGCGGCGTGGGCAATGCCGCGATCGCGGGCGCGGCGCTGGTGGGCGCCCGGCGCATCATCGCCGTCGACACCGACG
ACACCAAACTGGACTGGGCCCGCGGCTTCGGCGCCACCCACACCGTCAACGCCCGCCAGGCCGACGTGGTGGCCGCGATT
CAGGACCTCACCGACGGGTTCGGCGCCAACGTGGTGATCGACGCGGTGGGCCGCCCGGAAACCTGGAAGCAGGCCTTCTA
CGCCCGCGACCTGGCGGGAACCGTTGTGCTGGTGGGTGTTCCGACTCCCGACATGACGCTGGAGATGCCGCTGGTGGACT
TCTTCAGCCACGGCGGGTCGTTGAAGTCGTCCTGGTACGGCGACTGCCTGCCCGAACGCGACTTCCCCACGCTGATCGAG
CTGTATCTGCAGGGCCGGCTTCCCCTCGAAAAGTTCGTCTCCGAGCGCATCGGGCTCGGCGACGTCGAAGCCGCCTTCCA
CAACATGCACGACGGCAAGGTGCTGCGCTCGGTGGTGATGCTGTAA

Upstream 100 bases:

>100_bases
CGGTCGTCTCCAAAGCCGTCACGCCCAGGGACGCTACACCGGCTCGATGGGTGGACGGCCCGCCGCGAGATGTATGGCGG
ACGACAGTAGGTTGGAGCCC

Downstream 100 bases:

>100_bases
ATGGCCGGCATCGACAAGATCGTCACCCACGGCACCTTCGAACTCGACGGCGGCAGTTGGGAAGTCGACAACAACATCTG
GATCGTCGGCGACGACTCCG

Product: AdhE2

Products: NA

Alternate protein names: NAD/mycothiol-dependent formaldehyde dehydrogenase; MD-FALDH [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSQTVRGVISRKKGEPVELVDIVVPDPGPGEALVDVIACGVCHTDLTYREGGINDEYPFLLGHEAAGRVEAVGPDVTAVQ
PGDFVVLNWRAVCGQCRACKRGRPHLCFDTFNATQKMTLTDGTELTPALGIGAFADKTLVAAGQCTKVDPAADPAVAGLL
GCGVMAGLGAAINTGAVARDDTVAVIGCGGVGNAAIAGAALVGARRIIAVDTDDTKLDWARGFGATHTVNARQADVVAAI
QDLTDGFGANVVIDAVGRPETWKQAFYARDLAGTVVLVGVPTPDMTLEMPLVDFFSHGGSLKSSWYGDCLPERDFPTLIE
LYLQGRLPLEKFVSERIGLGDVEAAFHNMHDGKVLRSVVML

Sequences:

>Translated_361_residues
MSQTVRGVISRKKGEPVELVDIVVPDPGPGEALVDVIACGVCHTDLTYREGGINDEYPFLLGHEAAGRVEAVGPDVTAVQ
PGDFVVLNWRAVCGQCRACKRGRPHLCFDTFNATQKMTLTDGTELTPALGIGAFADKTLVAAGQCTKVDPAADPAVAGLL
GCGVMAGLGAAINTGAVARDDTVAVIGCGGVGNAAIAGAALVGARRIIAVDTDDTKLDWARGFGATHTVNARQADVVAAI
QDLTDGFGANVVIDAVGRPETWKQAFYARDLAGTVVLVGVPTPDMTLEMPLVDFFSHGGSLKSSWYGDCLPERDFPTLIE
LYLQGRLPLEKFVSERIGLGDVEAAFHNMHDGKVLRSVVML
>Mature_360_residues
SQTVRGVISRKKGEPVELVDIVVPDPGPGEALVDVIACGVCHTDLTYREGGINDEYPFLLGHEAAGRVEAVGPDVTAVQP
GDFVVLNWRAVCGQCRACKRGRPHLCFDTFNATQKMTLTDGTELTPALGIGAFADKTLVAAGQCTKVDPAADPAVAGLLG
CGVMAGLGAAINTGAVARDDTVAVIGCGGVGNAAIAGAALVGARRIIAVDTDDTKLDWARGFGATHTVNARQADVVAAIQ
DLTDGFGANVVIDAVGRPETWKQAFYARDLAGTVVLVGVPTPDMTLEMPLVDFFSHGGSLKSSWYGDCLPERDFPTLIEL
YLQGRLPLEKFVSERIGLGDVEAAFHNMHDGKVLRSVVML

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI34577061, Length=358, Percent_Identity=31.2849162011173, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI4501929, Length=358, Percent_Identity=31.2849162011173, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI71565154, Length=378, Percent_Identity=32.010582010582, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI4501933, Length=358, Percent_Identity=29.0502793296089, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI156523966, Length=370, Percent_Identity=30.8108108108108, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI262073058, Length=363, Percent_Identity=30.0275482093664, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI71743840, Length=363, Percent_Identity=30.0275482093664, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4501939, Length=362, Percent_Identity=30.939226519337, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI71565152, Length=372, Percent_Identity=29.5698924731183, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1786552, Length=368, Percent_Identity=34.2391304347826, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1790045, Length=359, Percent_Identity=25.0696378830084, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87081918, Length=334, Percent_Identity=29.3413173652695, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=26.3157894736842, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787863, Length=337, Percent_Identity=24.9258160237389, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786518, Length=290, Percent_Identity=28.9655172413793, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788407, Length=328, Percent_Identity=24.6951219512195, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87082125, Length=299, Percent_Identity=27.7591973244147, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788075, Length=355, Percent_Identity=26.7605633802817, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786825, Length=251, Percent_Identity=30.2788844621514, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI226510992, Length=356, Percent_Identity=24.438202247191, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790718, Length=323, Percent_Identity=23.2198142414861, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=391, Percent_Identity=30.690537084399, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71997431, Length=393, Percent_Identity=30.5343511450382, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=32.0699708454811, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17562582, Length=362, Percent_Identity=27.6243093922652, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562584, Length=368, Percent_Identity=29.6195652173913, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988145, Length=365, Percent_Identity=27.1232876712329, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17562878, Length=351, Percent_Identity=24.5014245014245, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=363, Percent_Identity=34.7107438016529, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319621, Length=352, Percent_Identity=29.5454545454545, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=369, Percent_Identity=27.9132791327913, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322619, Length=357, Percent_Identity=28.0112044817927, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324486, Length=287, Percent_Identity=28.2229965156794, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319955, Length=357, Percent_Identity=27.7310924369748, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=363, Percent_Identity=25.068870523416, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319257, Length=348, Percent_Identity=24.1379310344828, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319258, Length=324, Percent_Identity=24.0740740740741, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17737895, Length=378, Percent_Identity=32.5396825396825, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI17737897, Length=350, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17137530, Length=350, Percent_Identity=25.1428571428571, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR017816
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.306 [H]

Molecular weight: Translated: 37828; Mature: 37696

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTVRGVISRKKGEPVELVDIVVPDPGPGEALVDVIACGVCHTDLTYREGGINDEYPFL
CCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCEE
LGHEAAGRVEAVGPDVTAVQPGDFVVLNWRAVCGQCRACKRGRPHLCFDTFNATQKMTLT
ECCCCCCCEEECCCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEC
DGTELTPALGIGAFADKTLVAAGQCTKVDPAADPAVAGLLGCGVMAGLGAAINTGAVARD
CCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCEECC
DTVAVIGCGGVGNAAIAGAALVGARRIIAVDTDDTKLDWARGFGATHTVNARQADVVAAI
CCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCEECCCCHHHHHHHH
QDLTDGFGANVVIDAVGRPETWKQAFYARDLAGTVVLVGVPTPDMTLEMPLVDFFSHGGS
HHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEECHHHHHHHCCCC
LKSSWYGDCLPERDFPTLIELYLQGRLPLEKFVSERIGLGDVEAAFHNMHDGKVLRSVVM
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHC
L
C
>Mature Secondary Structure 
SQTVRGVISRKKGEPVELVDIVVPDPGPGEALVDVIACGVCHTDLTYREGGINDEYPFL
CHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCEE
LGHEAAGRVEAVGPDVTAVQPGDFVVLNWRAVCGQCRACKRGRPHLCFDTFNATQKMTLT
ECCCCCCCEEECCCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEC
DGTELTPALGIGAFADKTLVAAGQCTKVDPAADPAVAGLLGCGVMAGLGAAINTGAVARD
CCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCEECC
DTVAVIGCGGVGNAAIAGAALVGARRIIAVDTDDTKLDWARGFGATHTVNARQADVVAAI
CCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCEECCCCHHHHHHHH
QDLTDGFGANVVIDAVGRPETWKQAFYARDLAGTVVLVGVPTPDMTLEMPLVDFFSHGGS
HHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEECHHHHHHHCCCC
LKSSWYGDCLPERDFPTLIELYLQGRLPLEKFVSERIGLGDVEAAFHNMHDGKVLRSVVM
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9346279; 1597190; 9202149 [H]