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The map label for this gene is tcmG [H]

Identifier: 41408099

GI number: 41408099

Start: 2208108

End: 2209628

Strand: Reverse

Name: tcmG [H]

Synonym: MAP2001c

Alternate gene names: 41408099

Gene position: 2209628-2208108 (Counterclockwise)

Preceding gene: 41408100

Following gene: 41408091

Centisome position: 45.75

GC content: 69.56

Gene sequence:

>1521_bases
ATGACCACGCGAGTGCCTGTGCTCATCGTCGGGGCCGGCGTCGGCGGCCTGTCCATGTCGGCGCTGCTGACCCAACACGG
TGTCCGGTCGCTGCTGGTGGAGAAGCGGCGCGAGGTCTTCCTGTACCCGAAGGCCCGCAACCTGAGCTTTCGCAGCTTGG
AGATCCTGCGCGGGATGGGTCTGGGCGACGAGGTGCATGCGGTCGCCGAGCACGTCTCGACCGTGGTGGCCAAGCCGGCG
CTCAACAGCCCCGAGGAGCGACCGGCGCTCGACCTCGAGGGGATCTTCGCCGGTCTGGAGCGGTTCAGCCCCGAACCGGC
GGCCCAGTACTGCCCGCAGAGCCGGCTCGAGCCGATCCTGCTCGCCGCGACCCGGCGCGGCGGCAGCGAGGTGCGCTACC
GGACCGAGCTGTCCTCGATCGACATGGACGAGGCCGGCGTCACCGCGGTGGTTCGCGACGCCGACTCGGGCACCGCCGAG
ACGATCCGCGCCGACTATCTGATCGGCGCCGACGGGGTGCACAGTCCGATCCGTAACCTGTTGGGAATCACCACGTCTGG
ATACGGCGCGCTGCCCATCTACGTCGTCTTCGTCTACTTCCGGGCGCCCTGGCGAAAGTTCATCCCGCACTTGCACGACG
GCGACGGCGTGCAGGTCAAGAACGCCGACGTGGACGGCATCTTCCTGGTCGCCGAGGGTGACCTCGGCATGTTCATCACC
ACCTACTTTCCCGACGACGGCGAGACGGCCGAGCAGTTCACGCCGCAGCGCTGCCGCGAGCTGCTCACCAAGGCGATCGG
CGAGCCGCTCGACATCGACGTCATCGAGGTCGCGGCATGGCAGCCCTACGAGCAGGTCGCCGACCGATTCGATTGCGGGC
GAGCGTTTTTGGTGGGCGATTCGGCGCACACCATGCCGCCGTTCAAGGCCGGGGGAGCCAACACCGCGATCCAGAGCGCG
CACAACCTGGCCTGGAAACTCGCCGCCGTGCTGCGAGGCGCGGCCGGACCGGCGTTGCTGGACACCTACCACGCCGAGCG
CCACCCCGTCGGGGTCTTCGCCGCGCGCCAGTCGCTCACCGGGCCGTCGATGGCCCTGCTGAGACCGGGTGGGAAGCGGC
CGCAACTGCCGGCCGACGAAGAGGCACCGATCTTTGCCCTGATGATCGGATACCGCTACGGCGCGAACGGCATCGAGCTG
GTGGACGAGTTGCGCGCCCAGCCGGGCACCCGGGTGCCACACGTCTGGGTGCGCGACGGTGTCTCGACCCTCGACCTGGT
GGGCCCGGAGTTCACCGTGCTCACCGGCGACGAACGCTGGTGTGCCGCAGCGGTTTCCGTGTCGCTACCGGCGCACCGGG
TGTGCAACGACCAGTGGGCGGCGATCACCGGGCTGGCGCCGGACGGGGCACTGCTGATCCGGCCCGACGATTTCGTCGGG
TGGCGCGCCGACGAGCTGCCCACCGACCCGCAAACCGCTCTCCGCCAAGCGCTTTCGCTGTGCCTGGGCCGGGCACGTTA
G

Upstream 100 bases:

>100_bases
TCGGGTAGGGCGGCTGGTCACCCCCCACCCGTCAGGTATTGATAGTTCCTATCGAATGATAGAAACTGTCAGTTCTGGTG
GGGTGTCGAAAGGGGACGAG

Downstream 100 bases:

>100_bases
GGCGCGAGCAGACGCAAAAGCCCCTGATGCGCGGCGTGTCGCGGGGCTTTTGCGTCTGCTCGCGCGGGAAAAGTGGGGTT
ACAGCGGGATGTTCTTGTGA

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MTTRVPVLIVGAGVGGLSMSALLTQHGVRSLLVEKRREVFLYPKARNLSFRSLEILRGMGLGDEVHAVAEHVSTVVAKPA
LNSPEERPALDLEGIFAGLERFSPEPAAQYCPQSRLEPILLAATRRGGSEVRYRTELSSIDMDEAGVTAVVRDADSGTAE
TIRADYLIGADGVHSPIRNLLGITTSGYGALPIYVVFVYFRAPWRKFIPHLHDGDGVQVKNADVDGIFLVAEGDLGMFIT
TYFPDDGETAEQFTPQRCRELLTKAIGEPLDIDVIEVAAWQPYEQVADRFDCGRAFLVGDSAHTMPPFKAGGANTAIQSA
HNLAWKLAAVLRGAAGPALLDTYHAERHPVGVFAARQSLTGPSMALLRPGGKRPQLPADEEAPIFALMIGYRYGANGIEL
VDELRAQPGTRVPHVWVRDGVSTLDLVGPEFTVLTGDERWCAAAVSVSLPAHRVCNDQWAAITGLAPDGALLIRPDDFVG
WRADELPTDPQTALRQALSLCLGRAR

Sequences:

>Translated_506_residues
MTTRVPVLIVGAGVGGLSMSALLTQHGVRSLLVEKRREVFLYPKARNLSFRSLEILRGMGLGDEVHAVAEHVSTVVAKPA
LNSPEERPALDLEGIFAGLERFSPEPAAQYCPQSRLEPILLAATRRGGSEVRYRTELSSIDMDEAGVTAVVRDADSGTAE
TIRADYLIGADGVHSPIRNLLGITTSGYGALPIYVVFVYFRAPWRKFIPHLHDGDGVQVKNADVDGIFLVAEGDLGMFIT
TYFPDDGETAEQFTPQRCRELLTKAIGEPLDIDVIEVAAWQPYEQVADRFDCGRAFLVGDSAHTMPPFKAGGANTAIQSA
HNLAWKLAAVLRGAAGPALLDTYHAERHPVGVFAARQSLTGPSMALLRPGGKRPQLPADEEAPIFALMIGYRYGANGIEL
VDELRAQPGTRVPHVWVRDGVSTLDLVGPEFTVLTGDERWCAAAVSVSLPAHRVCNDQWAAITGLAPDGALLIRPDDFVG
WRADELPTDPQTALRQALSLCLGRAR
>Mature_505_residues
TTRVPVLIVGAGVGGLSMSALLTQHGVRSLLVEKRREVFLYPKARNLSFRSLEILRGMGLGDEVHAVAEHVSTVVAKPAL
NSPEERPALDLEGIFAGLERFSPEPAAQYCPQSRLEPILLAATRRGGSEVRYRTELSSIDMDEAGVTAVVRDADSGTAET
IRADYLIGADGVHSPIRNLLGITTSGYGALPIYVVFVYFRAPWRKFIPHLHDGDGVQVKNADVDGIFLVAEGDLGMFITT
YFPDDGETAEQFTPQRCRELLTKAIGEPLDIDVIEVAAWQPYEQVADRFDCGRAFLVGDSAHTMPPFKAGGANTAIQSAH
NLAWKLAAVLRGAAGPALLDTYHAERHPVGVFAARQSLTGPSMALLRPGGKRPQLPADEEAPIFALMIGYRYGANGIELV
DELRAQPGTRVPHVWVRDGVSTLDLVGPEFTVLTGDERWCAAAVSVSLPAHRVCNDQWAAITGLAPDGALLIRPDDFVGW
RADELPTDPQTALRQALSLCLGRAR

Specific function: Hydroxylates tetracenomycin A2 at positions C-4, C-4A, and C12A to give tetracenomycin C (TCM C). Hydroxylates as well 9- carboxymethyl-TCM B3 to yield 8-demethyl-TMC C, a side reaction [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=350, Percent_Identity=29.1428571428571, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 54695; Mature: 54564

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRVPVLIVGAGVGGLSMSALLTQHGVRSLLVEKRREVFLYPKARNLSFRSLEILRGMG
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCC
LGDEVHAVAEHVSTVVAKPALNSPEERPALDLEGIFAGLERFSPEPAAQYCPQSRLEPIL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHCCCEEE
LAATRRGGSEVRYRTELSSIDMDEAGVTAVVRDADSGTAETIRADYLIGADGVHSPIRNL
EEEECCCCCCEEEEEHHCCCCCCCCCCEEEEEECCCCCHHHEEEEEEECCCCHHHHHHHH
LGITTSGYGALPIYVVFVYFRAPWRKFIPHLHDGDGVQVKNADVDGIFLVAEGDLGMFIT
HCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEECCCCCEEEEEEECCCCEEEE
TYFPDDGETAEQFTPQRCRELLTKAIGEPLDIDVIEVAAWQPYEQVADRFDCGRAFLVGD
EEECCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEECC
SAHTMPPFKAGGANTAIQSAHNLAWKLAAVLRGAAGPALLDTYHAERHPVGVFAARQSLT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEHHHCCC
GPSMALLRPGGKRPQLPADEEAPIFALMIGYRYGANGIELVDELRAQPGTRVPHVWVRDG
CCCEEEECCCCCCCCCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHCCCCCCCCEEEECCC
VSTLDLVGPEFTVLTGDERWCAAAVSVSLPAHRVCNDQWAAITGLAPDGALLIRPDDFVG
CCEEECCCCCEEEEECCCCEEEEEEEECCCHHHHCCCCCEEEEECCCCCEEEECCCCCCC
WRADELPTDPQTALRQALSLCLGRAR
CCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTRVPVLIVGAGVGGLSMSALLTQHGVRSLLVEKRREVFLYPKARNLSFRSLEILRGMG
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCC
LGDEVHAVAEHVSTVVAKPALNSPEERPALDLEGIFAGLERFSPEPAAQYCPQSRLEPIL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHCCCEEE
LAATRRGGSEVRYRTELSSIDMDEAGVTAVVRDADSGTAETIRADYLIGADGVHSPIRNL
EEEECCCCCCEEEEEHHCCCCCCCCCCEEEEEECCCCCHHHEEEEEEECCCCHHHHHHHH
LGITTSGYGALPIYVVFVYFRAPWRKFIPHLHDGDGVQVKNADVDGIFLVAEGDLGMFIT
HCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEECCCCCEEEEEEECCCCEEEE
TYFPDDGETAEQFTPQRCRELLTKAIGEPLDIDVIEVAAWQPYEQVADRFDCGRAFLVGD
EEECCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEECC
SAHTMPPFKAGGANTAIQSAHNLAWKLAAVLRGAAGPALLDTYHAERHPVGVFAARQSLT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEHHHCCC
GPSMALLRPGGKRPQLPADEEAPIFALMIGYRYGANGIELVDELRAQPGTRVPHVWVRDG
CCCEEEECCCCCCCCCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHCCCCCCCCEEEECCC
VSTLDLVGPEFTVLTGDERWCAAAVSVSLPAHRVCNDQWAAITGLAPDGALLIRPDDFVG
CCEEECCCCCEEEEECCCCEEEEEEEECCCHHHHCCCCCEEEEECCCCCEEEECCCCCCC
WRADELPTDPQTALRQALSLCLGRAR
CCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]