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The map label for this gene is ilvE

Identifier: 41408048

GI number: 41408048

Start: 2150947

End: 2152062

Strand: Reverse

Name: ilvE

Synonym: MAP1950c

Alternate gene names: 41408048

Gene position: 2152062-2150947 (Counterclockwise)

Preceding gene: 41408049

Following gene: 41408043

Centisome position: 44.56

GC content: 68.82

Gene sequence:

>1116_bases
ATGACCAGTGGCTCCCTCGAGTTCACGGTTTCGCGCTCGACACGTCCGGCGACCGGCGCCGAACGCGAAACCATCCTGGC
CGAGCCGGGTTTCGGCAAATACTTCACCGACCACATGGTGTCGATCGAGTACGACGAGACCGAGGCCGGACGGGGCTGGC
ATGACGCGCGCGTCATCCCGTACGGCCCGATCGAGCTGGACCCGTCGGCGATCGTGTTGCACTACGCGCAGGAGATCTTC
GAAGGGCTCAAGGCCTACCGCTGGGCGGACGGCTCCATCGTGTCGTTTCGCGCCCAGGCCAACGCCGCCCGGTTGCGGTC
CTCGGCGCGGCGGCTGGCGATTCCCGAGCTGCCCGAGGAGCTGTTCCTGGAATCGCTGCGCCAGCTGATCGCCGTCGACA
ACGCCTGGGTGCCGGCCGCCGGCGGCGAAGAGGCGCTGTACCTGCGGCCGTTCGTCATCGCCACCGAGCCCGGGCTCGGG
GTGCGGCCCTCCAAGCAATACCGCTACCTGCTGATCGCCTCCCCGGCCGGCGCCTACTTCAAGGGCGGCATCAGTCCGGT
CACCGTCTGGGTGTCGACGGAGTACGTGCGGGCCAGCCCGGGCGGCACCGGGGCGGCCAAGTTCGGCGGCAACTACGCGG
CGTCGCTGTCCGCGCAAGCCGAGGCCGCGGCCAACGGGTGCGACCAGGTGGTGTGGCTGGACGCCGTCGAGCGGCGCTTT
GTCGAGGAGATGGGCGGGATGAACATCTTCTTCGTGTTCGGCAGCGGCGGCTCGGCACGGCTGGTCACCCCCGAGCTGTC
CGGCTCGCTGCTGCCCGGTATCACGCGAGATTCGTTGCTGCAGTTGGCAATTGACGCCGGCTTCGCCGTCGAGGAACGCA
AGATCGACATCGACGAGTGGCAGAAGAAGGCCGCCGCCGGTGAGATCACCGAGGTGTTCGCCTGCGGCACCGCCGCCGTC
ATCACGCCGGTGTCGCACGTCAAGTACGGCGAGACGGAGTTCACCATCGCCGACGGCCAGCCCGGCGAAGTGACGATGGC
ATTGCGCGACACGCTCACCGGGATCCAGCGGGGCACCTTCGCCGACACCCACGGCTGGATGACCCGGCTCGGCTGA

Upstream 100 bases:

>100_bases
GTGCGAGGTGGTGCGGCCCCCGTTCGTCGCGGTGAAAACCCGGTAGCGGCGCGGTCGCGCGGGCAAAATCCGATCGGACG
CCCGGATATACAATCAGCGG

Downstream 100 bases:

>100_bases
TTTGCGGGCTCGCGGCCTGCGGGCGCCGGCTGCCCGCGACTGTCGGCGCCCCGCCCGCGACTGTCGGCTCGCCCGCGACT
GTCGGCTCGCCTGCGAGCGT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTSGSLEFTVSRSTRPATGAERETILAEPGFGKYFTDHMVSIEYDETEAGRGWHDARVIPYGPIELDPSAIVLHYAQEIF
EGLKAYRWADGSIVSFRAQANAARLRSSARRLAIPELPEELFLESLRQLIAVDNAWVPAAGGEEALYLRPFVIATEPGLG
VRPSKQYRYLLIASPAGAYFKGGISPVTVWVSTEYVRASPGGTGAAKFGGNYAASLSAQAEAAANGCDQVVWLDAVERRF
VEEMGGMNIFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERKIDIDEWQKKAAAGEITEVFACGTAAV
ITPVSHVKYGETEFTIADGQPGEVTMALRDTLTGIQRGTFADTHGWMTRLG

Sequences:

>Translated_371_residues
MTSGSLEFTVSRSTRPATGAERETILAEPGFGKYFTDHMVSIEYDETEAGRGWHDARVIPYGPIELDPSAIVLHYAQEIF
EGLKAYRWADGSIVSFRAQANAARLRSSARRLAIPELPEELFLESLRQLIAVDNAWVPAAGGEEALYLRPFVIATEPGLG
VRPSKQYRYLLIASPAGAYFKGGISPVTVWVSTEYVRASPGGTGAAKFGGNYAASLSAQAEAAANGCDQVVWLDAVERRF
VEEMGGMNIFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERKIDIDEWQKKAAAGEITEVFACGTAAV
ITPVSHVKYGETEFTIADGQPGEVTMALRDTLTGIQRGTFADTHGWMTRLG
>Mature_370_residues
TSGSLEFTVSRSTRPATGAERETILAEPGFGKYFTDHMVSIEYDETEAGRGWHDARVIPYGPIELDPSAIVLHYAQEIFE
GLKAYRWADGSIVSFRAQANAARLRSSARRLAIPELPEELFLESLRQLIAVDNAWVPAAGGEEALYLRPFVIATEPGLGV
RPSKQYRYLLIASPAGAYFKGGISPVTVWVSTEYVRASPGGTGAAKFGGNYAASLSAQAEAAANGCDQVVWLDAVERRFV
EEMGGMNIFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERKIDIDEWQKKAAAGEITEVFACGTAAVI
TPVSHVKYGETEFTIADGQPGEVTMALRDTLTGIQRGTFADTHGWMTRLG

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=333, Percent_Identity=40.5405405405405, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI296010906, Length=333, Percent_Identity=40.5405405405405, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI38176287, Length=333, Percent_Identity=40.5405405405405, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI50658084, Length=368, Percent_Identity=37.7717391304348, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=38.7755102040816, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI296010900, Length=333, Percent_Identity=36.036036036036, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI296010902, Length=333, Percent_Identity=36.036036036036, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI48994963, Length=329, Percent_Identity=29.483282674772, Blast_Score=118, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17568601, Length=348, Percent_Identity=37.9310344827586, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17565728, Length=367, Percent_Identity=34.6049046321526, Blast_Score=191, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6322002, Length=349, Percent_Identity=38.9684813753582, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6322608, Length=350, Percent_Identity=37.7142857142857, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24641779, Length=343, Percent_Identity=40.8163265306122, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39981; Mature: 39850

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGSLEFTVSRSTRPATGAERETILAEPGFGKYFTDHMVSIEYDETEAGRGWHDARVIP
CCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCHHCCEEEEEECCCCCCCCCCCCEEEE
YGPIELDPSAIVLHYAQEIFEGLKAYRWADGSIVSFRAQANAARLRSSARRLAIPELPEE
CCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHH
LFLESLRQLIAVDNAWVPAAGGEEALYLRPFVIATEPGLGVRPSKQYRYLLIASPAGAYF
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCHH
KGGISPVTVWVSTEYVRASPGGTGAAKFGGNYAASLSAQAEAAANGCDQVVWLDAVERRF
CCCCCEEEEEEEEEEEEECCCCCCCHHCCCCEEEECCCHHHHHHCCCCCEEEHHHHHHHH
VEEMGGMNIFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERKIDIDEW
HHHHCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHH
QKKAAAGEITEVFACGTAAVITPVSHVKYGETEFTIADGQPGEVTMALRDTLTGIQRGTF
HHHHHCCCHHHHHHCCCHHEECCHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHHHCCCC
ADTHGWMTRLG
CCCCCHHHCCC
>Mature Secondary Structure 
TSGSLEFTVSRSTRPATGAERETILAEPGFGKYFTDHMVSIEYDETEAGRGWHDARVIP
CCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCHHCCEEEEEECCCCCCCCCCCCEEEE
YGPIELDPSAIVLHYAQEIFEGLKAYRWADGSIVSFRAQANAARLRSSARRLAIPELPEE
CCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHH
LFLESLRQLIAVDNAWVPAAGGEEALYLRPFVIATEPGLGVRPSKQYRYLLIASPAGAYF
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCHH
KGGISPVTVWVSTEYVRASPGGTGAAKFGGNYAASLSAQAEAAANGCDQVVWLDAVERRF
CCCCCEEEEEEEEEEEEECCCCCCCHHCCCCEEEECCCHHHHHHCCCCCEEEHHHHHHHH
VEEMGGMNIFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERKIDIDEW
HHHHCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHH
QKKAAAGEITEVFACGTAAVITPVSHVKYGETEFTIADGQPGEVTMALRDTLTGIQRGTF
HHHHHCCCHHHHHHCCCHHEECCHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHHHCCCC
ADTHGWMTRLG
CCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]