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The map label for this gene is fadD31

Identifier: 41407745

GI number: 41407745

Start: 1800662

End: 1802500

Strand: Direct

Name: fadD31

Synonym: MAP1647

Alternate gene names: 41407745

Gene position: 1800662-1802500 (Clockwise)

Preceding gene: 41407743

Following gene: 41407746

Centisome position: 37.28

GC content: 70.64

Gene sequence:

>1839_bases
ATGGATGACGCCCTTCGGCGCGACGACGGTGTGCCCGGCCTGCTCCGGATCGAGGACTGCCTGGACGCCGACGGCGGGGT
CGCACTGCCGCCCGGCGTCAACCTGATTTCGCTCATCGACCGCAACATCGCCAACGTGGGGGACACCGTCGCGTATCGCT
ACCTCGACTACAGCGGTTCGGACGACGGCACCGCGCACGAGGTGACCTGGAGCCAGTTCGGTGTCCGGCTGGAAGCCATC
GGCGCGCGCATCCAGCAGGTGGCCAGTCGCGGTGAGCGCGTTGCGGTGCTGGCGCCGCAGGGCATCGACTACGTCGCCGG
GTTCTACGCGGCGGTCAAGGCGGGCACCATCGCGGTCCCGTTGTTCGCCCCGGAACTGCCCGGGCATACCGAGCGGCTGG
ACACGGCGCTGCGCGATTCGCAGCCCAGCGTCCTGCTCACCACCACCGTCGCGCGGGATGCGGTCGAGCAGTTCCTGGCC
GGCCACCCGCACCTGCACCGGCCGCGGGTGATCGCCATCGACGAGATACCCGACTCGGCCGCCGAGTCGTTCGCGCCGAC
CGAACTCGGCATGGACGACGTCTCGCATCTGCAGTACACGTCGGGCTCCACCCGGCCACCGGTCGGCGTGGAGATCACCC
ACCGCGCGGTCGGCACCAACCTGGTGCAGATGATCCTGTCCATCGACCTGCTGGACCGAAACACCCACGGCGTCAGCTGG
TTACCGCTCTACCACGACATGGGGTTGTCGATGATCGGCTTCCCCGCGGTGTACGGCGGCCACTCCACGCTGATGTCACC
CGCGGCGTTCGTCCGCCGGCCGCAGCGCTGGATCAAGGCCCTGTCCGACGGGTCGCGGCACGGCAACGTCGTCACCGCGG
CGCCCAACTTCGCCTACGAGTGGGCCGCGCAGCGCGGGCTGCCCGGCGGCGGCGAGGACATCAACCTGCGCAATGTGGTG
ATGATCATCGGCTCCGAACCGGTCAGCATGGATGCGATCCGGACTTTCAACAAGGCGTTCGCTCCATACGGGTTGCCGCG
GACGGCGTTCAAGCCGTCCTACGGCATCGCCGAGGCGACGCTGTTCGTTGCGACCATCGCGCCGCAGGCCGAGGCGACGG
CGGTGTATTTCGACCGCCGGCAGTTGGGCGCCGGACATGCGGTGCGGGTGCCGGCCAACGCCCCCGATGCCGTCGCCGCG
GTGTCCTGTGGCCAGGTGGCGCGGAGTGAATGGGCGGTGATCGTCGCCGACGGGGCCGAGTTGCCGGACGGCCAGGTGGG
CGAAATCTGGTTGCAGGGCAACAACATTGGCCGCGGCTACTGGGGAATGCCGGAGGAGACTCGGCGGGTGTTCGGCGCCA
CGCTGCGGTCGCGGCTGCCGGACGGCAGCCACGCCGCGGGGGCCGCTCCCGACGGCAGCTGGCTGCGCACCGGCGATTTG
GGTGTGTATCTCGACGGCGAGCTGTACGTGACGGGCCGGATCGCGGACCTGGTGCGCATCGATGGCCGCAACCACTATCC
GCAGGACATCGAGGCCACCGTCGCCGAGGCGTCGCCGATGGTTCGGCGTGGTTATGTGACGGCGTTCTCGGTGCCGGCCG
TGGACGATTCCGAAGCGGGCCGGCTGGTCGTGGTCGCCGAGCGCGCTGCGGGCACCAGCCGTCAGGACCCGCGGCCGGCG
ATCGAGGTCATCCGCGCCGCCGTCGCGCAGCGGCACGAGTTGGCCGTCGCCGACGTGCGGCTGCTGCCCGCCGGCGCCAT
CCCGCGGACTACCAGCGGCAAGCTGGCCCGGCGGGCCTGCCGCGCCCAATACCTCGACGGCAGCCTAGGCGCCCGCTGA

Upstream 100 bases:

>100_bases
CGCGCTACCGCCGGAATGAGCGAATACGGCCGTCGCTCTTGTGGTTTGCCCGAACGTCACCGACCCCGGCCGGCCGCATC
GCGTGCCTAGACTGCACGGT

Downstream 100 bases:

>100_bases
TCGGGCGGGTCGACGGCACCGCACCGGCCCGGCGCCGGTGGCATCCTGGCCAGGTGGGGTTGCTGATCAGAGTGGCCGAG
CTGTTGATCCTGGTGCTGCC

Product: putative fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MDDALRRDDGVPGLLRIEDCLDADGGVALPPGVNLISLIDRNIANVGDTVAYRYLDYSGSDDGTAHEVTWSQFGVRLEAI
GARIQQVASRGERVAVLAPQGIDYVAGFYAAVKAGTIAVPLFAPELPGHTERLDTALRDSQPSVLLTTTVARDAVEQFLA
GHPHLHRPRVIAIDEIPDSAAESFAPTELGMDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLDRNTHGVSW
LPLYHDMGLSMIGFPAVYGGHSTLMSPAAFVRRPQRWIKALSDGSRHGNVVTAAPNFAYEWAAQRGLPGGGEDINLRNVV
MIIGSEPVSMDAIRTFNKAFAPYGLPRTAFKPSYGIAEATLFVATIAPQAEATAVYFDRRQLGAGHAVRVPANAPDAVAA
VSCGQVARSEWAVIVADGAELPDGQVGEIWLQGNNIGRGYWGMPEETRRVFGATLRSRLPDGSHAAGAAPDGSWLRTGDL
GVYLDGELYVTGRIADLVRIDGRNHYPQDIEATVAEASPMVRRGYVTAFSVPAVDDSEAGRLVVVAERAAGTSRQDPRPA
IEVIRAAVAQRHELAVADVRLLPAGAIPRTTSGKLARRACRAQYLDGSLGAR

Sequences:

>Translated_612_residues
MDDALRRDDGVPGLLRIEDCLDADGGVALPPGVNLISLIDRNIANVGDTVAYRYLDYSGSDDGTAHEVTWSQFGVRLEAI
GARIQQVASRGERVAVLAPQGIDYVAGFYAAVKAGTIAVPLFAPELPGHTERLDTALRDSQPSVLLTTTVARDAVEQFLA
GHPHLHRPRVIAIDEIPDSAAESFAPTELGMDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLDRNTHGVSW
LPLYHDMGLSMIGFPAVYGGHSTLMSPAAFVRRPQRWIKALSDGSRHGNVVTAAPNFAYEWAAQRGLPGGGEDINLRNVV
MIIGSEPVSMDAIRTFNKAFAPYGLPRTAFKPSYGIAEATLFVATIAPQAEATAVYFDRRQLGAGHAVRVPANAPDAVAA
VSCGQVARSEWAVIVADGAELPDGQVGEIWLQGNNIGRGYWGMPEETRRVFGATLRSRLPDGSHAAGAAPDGSWLRTGDL
GVYLDGELYVTGRIADLVRIDGRNHYPQDIEATVAEASPMVRRGYVTAFSVPAVDDSEAGRLVVVAERAAGTSRQDPRPA
IEVIRAAVAQRHELAVADVRLLPAGAIPRTTSGKLARRACRAQYLDGSLGAR
>Mature_612_residues
MDDALRRDDGVPGLLRIEDCLDADGGVALPPGVNLISLIDRNIANVGDTVAYRYLDYSGSDDGTAHEVTWSQFGVRLEAI
GARIQQVASRGERVAVLAPQGIDYVAGFYAAVKAGTIAVPLFAPELPGHTERLDTALRDSQPSVLLTTTVARDAVEQFLA
GHPHLHRPRVIAIDEIPDSAAESFAPTELGMDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLDRNTHGVSW
LPLYHDMGLSMIGFPAVYGGHSTLMSPAAFVRRPQRWIKALSDGSRHGNVVTAAPNFAYEWAAQRGLPGGGEDINLRNVV
MIIGSEPVSMDAIRTFNKAFAPYGLPRTAFKPSYGIAEATLFVATIAPQAEATAVYFDRRQLGAGHAVRVPANAPDAVAA
VSCGQVARSEWAVIVADGAELPDGQVGEIWLQGNNIGRGYWGMPEETRRVFGATLRSRLPDGSHAAGAAPDGSWLRTGDL
GVYLDGELYVTGRIADLVRIDGRNHYPQDIEATVAEASPMVRRGYVTAFSVPAVDDSEAGRLVVVAERAAGTSRQDPRPA
IEVIRAAVAQRHELAVADVRLLPAGAIPRTTSGKLARRACRAQYLDGSLGAR

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI225735629, Length=596, Percent_Identity=23.993288590604, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI45827692, Length=596, Percent_Identity=23.993288590604, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI44888818, Length=601, Percent_Identity=23.2945091514143, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI55749758, Length=558, Percent_Identity=22.5806451612903, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI225735625, Length=565, Percent_Identity=23.1858407079646, Blast_Score=80, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193202679, Length=579, Percent_Identity=23.3160621761658, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24654656, Length=434, Percent_Identity=23.963133640553, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 65385; Mature: 65385

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDALRRDDGVPGLLRIEDCLDADGGVALPPGVNLISLIDRNIANVGDTVAYRYLDYSGS
CCCCCCCCCCCCCEEEEHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCC
DDGTAHEVTWSQFGVRLEAIGARIQQVASRGERVAVLAPQGIDYVAGFYAAVKAGTIAVP
CCCCEEEEEHHHHCEEHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCEEEEE
LFAPELPGHTERLDTALRDSQPSVLLTTTVARDAVEQFLAGHPHLHRPRVIAIDEIPDSA
EECCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHH
AESFAPTELGMDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLDRNTHGVSW
HHHCCCCCCCCHHHHHEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEE
LPLYHDMGLSMIGFPAVYGGHSTLMSPAAFVRRPQRWIKALSDGSRHGNVVTAAPNFAYE
EEHHHHCCCCCCCCCCEECCCHHHHCCHHHHHCHHHHHHHHHCCCCCCCEEEECCCCHHH
WAAQRGLPGGGEDINLRNVVMIIGSEPVSMDAIRTFNKAFAPYGLPRTAFKPSYGIAEAT
HHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHE
LFVATIAPQAEATAVYFDRRQLGAGHAVRVPANAPDAVAAVSCGQVARSEWAVIVADGAE
EEEEEECCCCCCEEEEEEHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCC
LPDGQVGEIWLQGNNIGRGYWGMPEETRRVFGATLRSRLPDGSHAAGAAPDGSWLRTGDL
CCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCE
GVYLDGELYVTGRIADLVRIDGRNHYPQDIEATVAEASPMVRRGYVTAFSVPAVDDSEAG
EEEECCEEEEEEEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHCEEEEEECCCCCCCCCC
RLVVVAERAAGTSRQDPRPAIEVIRAAVAQRHELAVADVRLLPAGAIPRTTSGKLARRAC
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHH
RAQYLDGSLGAR
HHHHCCCCCCCC
>Mature Secondary Structure
MDDALRRDDGVPGLLRIEDCLDADGGVALPPGVNLISLIDRNIANVGDTVAYRYLDYSGS
CCCCCCCCCCCCCEEEEHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCC
DDGTAHEVTWSQFGVRLEAIGARIQQVASRGERVAVLAPQGIDYVAGFYAAVKAGTIAVP
CCCCEEEEEHHHHCEEHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCEEEEE
LFAPELPGHTERLDTALRDSQPSVLLTTTVARDAVEQFLAGHPHLHRPRVIAIDEIPDSA
EECCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHH
AESFAPTELGMDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLDRNTHGVSW
HHHCCCCCCCCHHHHHEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEE
LPLYHDMGLSMIGFPAVYGGHSTLMSPAAFVRRPQRWIKALSDGSRHGNVVTAAPNFAYE
EEHHHHCCCCCCCCCCEECCCHHHHCCHHHHHCHHHHHHHHHCCCCCCCEEEECCCCHHH
WAAQRGLPGGGEDINLRNVVMIIGSEPVSMDAIRTFNKAFAPYGLPRTAFKPSYGIAEAT
HHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHE
LFVATIAPQAEATAVYFDRRQLGAGHAVRVPANAPDAVAAVSCGQVARSEWAVIVADGAE
EEEEEECCCCCCEEEEEEHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCC
LPDGQVGEIWLQGNNIGRGYWGMPEETRRVFGATLRSRLPDGSHAAGAAPDGSWLRTGDL
CCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCE
GVYLDGELYVTGRIADLVRIDGRNHYPQDIEATVAEASPMVRRGYVTAFSVPAVDDSEAG
EEEECCEEEEEEEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHCEEEEEECCCCCCCCCC
RLVVVAERAAGTSRQDPRPAIEVIRAAVAQRHELAVADVRLLPAGAIPRTTSGKLARRAC
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHH
RAQYLDGSLGAR
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]