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The map label for this gene is fadE28

Identifier: 41406621

GI number: 41406621

Start: 549244

End: 550332

Strand: Direct

Name: fadE28

Synonym: MAP0523

Alternate gene names: 41406621

Gene position: 549244-550332 (Clockwise)

Preceding gene: 41406620

Following gene: 41406622

Centisome position: 11.37

GC content: 70.06

Gene sequence:

>1089_bases
ATGGCGTCAGAAACGACGATGGACTTCGACCCCAGTCCGACACAACAGGCGGTCGCCGACGTCGTCACGTCGGTGCTGGA
CCGCGAATTGAGTTGGGAGGCACTGGTCGACGGGGGTGTGACGGCGCTGCCGGTGCCCGAGCGTCTCGGCGGCGACGGGG
TGGGTCTGCCCGAGGTCGCCACGGTGCTCACCGAAGTCGGTCGCCGCGGCGCCATCACGCCGGCGCTGGCCACGTTGGGT
TTTGCCGTGCTTCCGTTGCTCGAGCTGGCCTCCGAGGAGCAGCAGGACCGGTTCCTGGCCGGGGTCGCCAGGGGCGGGGT
GCTGACGGCCGCGCTCAACGAACCCGGTACCCCGCTGCCGGACCGGCCCGCGACCACCTTCGCCGACGGCCGGTTGTCGG
GCACCAAGATCGGCGTCGGCTATGCGGCACAAGCGGATTGGATGATCGTCACCGCCGACAGTGCGGTTGTCGTGGTCTCG
CCCAAGGCCGACGGCGTCCAGGTGGTGCAGACGCCGACCTCGAACGGCTCCGACGAGTACACGGTCAGCTTCACCGGCGT
CGCGGTGGCCGATTCCGACGTGCTGGCGGGCGCCACGGCGGCGCGGGTCAACCAGCTGGCGCTGGCGGCCGTGGGCGCCT
ACGCCGACGGGCTGGTCAGCGGGGCGCTGCGGTTGACCGCCGACTACGTGGCCAACCGCAAGCAGTTCGGCAAGCCGCTG
TCCACCTTCCAGACCGTGGCCGCGCAGCTCGCCGAGGTCTACATCGCCTCGCGCACCATCGATTTGGTGGCCAAGTCGGT
GGTGTGGGGGCTGTCGGAGGGCCGGGATGTCGATCACGACCTGGGCGTGCTGGGATACTGGGTGGCGTCGCAGGCGCCGC
CGGCGATGCAGCTCTGCCACCACCTGCACGGCGGCATGGGCATGGACATCACCTATCCGATGCATCGGTACTACTCCACG
ATCAAGGACCTGACCCGGCTGCTGGGCGGGCCGTCGCATCGCCTGGACCTCGTGGCGATCGCAAGCGCGGCGCAGCCGGG
CGCAGCGGGTCGGCACGCAGACGACCTGGTGGGAGCGCAATGTTCATAG

Upstream 100 bases:

>100_bases
AAGCCGCTGTCGGCGCCCGAGCGGTTGCGGTCGGGCTGGCTCAACGGCATCAAGCACTGGCAGGTCGACTACACCGGCAA
GTGCCCGGTGGCGCACTGAG

Downstream 100 bases:

>100_bases
ACCTGACCCCCGAACAGCGTCAGCTGCAAGCCGAACTGCGGGAATACTTTTCGAACCTGATCTCGTCCGACGAGATGAAG
GCGATGGAGCAGGACCGCCA

Product: FadE28

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MASETTMDFDPSPTQQAVADVVTSVLDRELSWEALVDGGVTALPVPERLGGDGVGLPEVATVLTEVGRRGAITPALATLG
FAVLPLLELASEEQQDRFLAGVARGGVLTAALNEPGTPLPDRPATTFADGRLSGTKIGVGYAAQADWMIVTADSAVVVVS
PKADGVQVVQTPTSNGSDEYTVSFTGVAVADSDVLAGATAARVNQLALAAVGAYADGLVSGALRLTADYVANRKQFGKPL
STFQTVAAQLAEVYIASRTIDLVAKSVVWGLSEGRDVDHDLGVLGYWVASQAPPAMQLCHHLHGGMGMDITYPMHRYYST
IKDLTRLLGGPSHRLDLVAIASAAQPGAAGRHADDLVGAQCS

Sequences:

>Translated_362_residues
MASETTMDFDPSPTQQAVADVVTSVLDRELSWEALVDGGVTALPVPERLGGDGVGLPEVATVLTEVGRRGAITPALATLG
FAVLPLLELASEEQQDRFLAGVARGGVLTAALNEPGTPLPDRPATTFADGRLSGTKIGVGYAAQADWMIVTADSAVVVVS
PKADGVQVVQTPTSNGSDEYTVSFTGVAVADSDVLAGATAARVNQLALAAVGAYADGLVSGALRLTADYVANRKQFGKPL
STFQTVAAQLAEVYIASRTIDLVAKSVVWGLSEGRDVDHDLGVLGYWVASQAPPAMQLCHHLHGGMGMDITYPMHRYYST
IKDLTRLLGGPSHRLDLVAIASAAQPGAAGRHADDLVGAQCS
>Mature_361_residues
ASETTMDFDPSPTQQAVADVVTSVLDRELSWEALVDGGVTALPVPERLGGDGVGLPEVATVLTEVGRRGAITPALATLGF
AVLPLLELASEEQQDRFLAGVARGGVLTAALNEPGTPLPDRPATTFADGRLSGTKIGVGYAAQADWMIVTADSAVVVVSP
KADGVQVVQTPTSNGSDEYTVSFTGVAVADSDVLAGATAARVNQLALAAVGAYADGLVSGALRLTADYVANRKQFGKPLS
TFQTVAAQLAEVYIASRTIDLVAKSVVWGLSEGRDVDHDLGVLGYWVASQAPPAMQLCHHLHGGMGMDITYPMHRYYSTI
KDLTRLLGGPSHRLDLVAIASAAQPGAAGRHADDLVGAQCS

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17508101, Length=330, Percent_Identity=23.3333333333333, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356377, Length=365, Percent_Identity=23.2876712328767, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 37459; Mature: 37327

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASETTMDFDPSPTQQAVADVVTSVLDRELSWEALVDGGVTALPVPERLGGDGVGLPEVA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCHHHCCCCCCCHHHHH
TVLTEVGRRGAITPALATLGFAVLPLLELASEEQQDRFLAGVARGGVLTAALNEPGTPLP
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCC
DRPATTFADGRLSGTKIGVGYAAQADWMIVTADSAVVVVSPKADGVQVVQTPTSNGSDEY
CCCCCCCCCCCCCCCEEECCEECCCCEEEEEECCEEEEEECCCCCEEEEECCCCCCCCCE
TVSFTGVAVADSDVLAGATAARVNQLALAAVGAYADGLVSGALRLTADYVANRKQFGKPL
EEEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
STFQTVAAQLAEVYIASRTIDLVAKSVVWGLSEGRDVDHDLGVLGYWVASQAPPAMQLCH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
HLHGGMGMDITYPMHRYYSTIKDLTRLLGGPSHRLDLVAIASAAQPGAAGRHADDLVGAQ
HHCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCC
CS
CC
>Mature Secondary Structure 
ASETTMDFDPSPTQQAVADVVTSVLDRELSWEALVDGGVTALPVPERLGGDGVGLPEVA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCHHHCCCCCCCHHHHH
TVLTEVGRRGAITPALATLGFAVLPLLELASEEQQDRFLAGVARGGVLTAALNEPGTPLP
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCC
DRPATTFADGRLSGTKIGVGYAAQADWMIVTADSAVVVVSPKADGVQVVQTPTSNGSDEY
CCCCCCCCCCCCCCCEEECCEECCCCEEEEEECCEEEEEECCCCCEEEEECCCCCCCCCE
TVSFTGVAVADSDVLAGATAARVNQLALAAVGAYADGLVSGALRLTADYVANRKQFGKPL
EEEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
STFQTVAAQLAEVYIASRTIDLVAKSVVWGLSEGRDVDHDLGVLGYWVASQAPPAMQLCH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
HLHGGMGMDITYPMHRYYSTIKDLTRLLGGPSHRLDLVAIASAAQPGAAGRHADDLVGAQ
HHCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCC
CS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]