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The map label for this gene is trmE

Identifier: 52843194

GI number: 52843194

Start: 3393934

End: 3395274

Strand: Reverse

Name: trmE

Synonym: lpg3001

Alternate gene names: 52843194

Gene position: 3395274-3393934 (Counterclockwise)

Preceding gene: 52843195

Following gene: 52843193

Centisome position: 99.93

GC content: 38.11

Gene sequence:

>1341_bases
ATGTCAATAGATACCATTGTTGCTATCGCTACCCCGCCAGGACGAGGCGGGGTAGGAATCGTCAGAATATCAGGTCCAAA
TGCTTATGCTATAGCCCTTTGTCTAAATGGGAACAAAGCATTGCAACCACGTTTAGCCACTTTTTGCTCTTTATATAAAG
GAAATAATGAAGTCCTTGATCAAGGATTGGTTCTTTATTTTAAAGGACCTCATTCTTTTACAGGTGAGGATGTTATTGAA
ATTCAAGCGCATGGCTCACCTGTAGTTTTGGATTTATTAATTAAGGAATCCATAGCAGCAGGCGCAAGGCTAGCCAGGCC
GGGTGAGTTTTCTGAAAGAGCATTTCTTAATGATAAAATCGATTTAATTCAAGCAGAAGCCATAGCTGATTTAATTCAAG
CCAGTTCAGATACTGCGGCTCGTATGGCTTTAAAATCACTGCAAGGTGATTTTTCTAAAAAAATAAATCAATTGAATGAA
GAACTAATTTACTTACGCATGTATGTTGAAGCTGCTATCGATTTTCCTGAAGAGGAAATTGATTTTCTTAATGATGGAAA
TGTCAGCCAATTACTACAAAAAATTATTGGACGATTAGAAGATATACGTTCACAAGCGAATCAAGGAGTTTTGTTGCGTG
AAGGGTTATCCTTGGTTATTGCTGGCAGGCCTAATGCAGGAAAATCAACCTTAATTAACAACCTGGCAGGGCGGGATGTT
GCCATTGTTACTGAAATTGCCGGTACTACGCGAGATATTATGCGAGAACATATCCTATTGGATGATATCCCTCTACACAT
TATCGATACGGCAGGATTAAGAGACAGCGATGATCTGGTGGAGAAAGAGGGAATAAAACGTGCTTGGCAAGAGTTGAAGC
GAGCTGATTGTGTGCTTCTTGTAGTAGATATTAACAATCCTGATCAACAAAATTCTCTGCTTAATGAATTGAGATTAACA
TTACCCAATAAAATACCCATAATCACGGTATACAATAAAATTGATACTACTAAACTTACAGCAAAATGTGATGAGCATAC
AGTTTATTTGTCTGCAAAAACTGGTGAGGGACTGGATGAGCTAAAAAAGGTGATTAAACAAGTGGTTGGATATCAACCAA
CAGAAGGCCAATTTTTAGCTCGCAGACGACATCTACAAGCGTTGGATGAAGCAAAAGCTTTGTTACTAACTGGGCAATCG
CAGCTAACAAATCATAAAGCTGGAGAATTGTTGGCAGAAGATTTACGGTTAGCTCATCAGACTTTATGTGAAATTACAGG
TGAGTTCACTTCAGACGATTTATTAGGAAAAATTTTTTCCAGTTTTTGTATTGGAAAATAA

Upstream 100 bases:

>100_bases
GATTGTGAATAATACTTTATCAATATTACAGCAATGGTACATCACCAGAAAATACTCAGATGAAAAGCCAGCTAAAAAGG
TTGTTGCCACAGCAAAATAA

Downstream 100 bases:

>100_bases
CGATGATGCAAGGCAGTCATCATGTGAATTTTCAATTTTTCTTCTTTTGAATACAGCAAAATTATTATTAAATATTTTGT
GGTGATGTGCTTTATTGGGT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIE
IQAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQKIIGRLEDIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDV
AIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLT
LPNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQS
QLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK

Sequences:

>Translated_446_residues
MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIE
IQAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQKIIGRLEDIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDV
AIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLT
LPNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQS
QLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK
>Mature_445_residues
SIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIEI
QAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNEE
LIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQKIIGRLEDIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDVA
IVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLTL
PNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQSQ
LTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=471, Percent_Identity=34.8195329087049, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI193082993, Length=462, Percent_Identity=35.0649350649351, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=31.9919517102616, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=59.8669623059867, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082120, Length=125, Percent_Identity=36.8, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=454, Percent_Identity=29.5154185022026, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=496, Percent_Identity=32.0564516129032, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI45550813, Length=478, Percent_Identity=33.6820083682008, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 48878; Mature: 48747

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLD
CCCCEEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHC
QGLVLYFKGPHSFTGEDVIEIQAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKI
CCEEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCH
DLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNEELIYLRMYVEAAIDFPEEEI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DFLNDGNVSQLLQKIIGRLEDIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDV
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHCCCCCE
AIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLL
EEEEHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
VVDINNPDQQNSLLNELRLTLPNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDE
EEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCEEEEEEECCCCCHHH
LKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQSQLTNHKAGELLAEDLRLAHQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHCCCHHHHHHHHHHHHHHH
TLCEITGEFTSDDLLGKIFSSFCIGK
HHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLD
CCCEEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHC
QGLVLYFKGPHSFTGEDVIEIQAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKI
CCEEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCH
DLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNEELIYLRMYVEAAIDFPEEEI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DFLNDGNVSQLLQKIIGRLEDIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDV
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHCCCCCE
AIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLL
EEEEHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
VVDINNPDQQNSLLNELRLTLPNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDE
EEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCEEEEEEECCCCCHHH
LKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQSQLTNHKAGELLAEDLRLAHQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHCCCHHHHHHHHHHHHHHH
TLCEITGEFTSDDLLGKIFSSFCIGK
HHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA