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The map label for this gene is gidB

Identifier: 52843085

GI number: 52843085

Start: 3272724

End: 3273350

Strand: Direct

Name: gidB

Synonym: lpg2890

Alternate gene names: 52843085

Gene position: 3272724-3273350 (Clockwise)

Preceding gene: 52843084

Following gene: 52843086

Centisome position: 96.32

GC content: 40.51

Gene sequence:

>627_bases
ATGATAGATAATACGAAAATAAGATCATTGCTTGAAAAGGGTTTAGCCGAGTTTAAACTCGATTCCATCGGCGACCTCTT
GCTTGATTTTTTGCTCCTGCTCAATAAATGGAATAAAACATATAACCTCACTGCAATACGTGATATTGAAACCATGGTCA
GCAAGCACCTGTTCGACAGTCTGGCTATTTTACCCTGGATAAAGGGAAACCATATTATCGATGTGGGTACTGGCCCGGGT
TTACCTGGCATCCCTTTGGCTATTGCCAAACCTGACTTACAATTTGTATTATTGGACAGTAATGGGAAAAAAATTCGCTT
TCTCAATGAAGTAAAACGTCAACTCAATATAAAAAACATTGAACCAATACAAATTCGAGTAGAGAACTACCACCCAAACC
AAGGTTTTGATACAGTAATAAGCAGAGCATTTAGCAGTCTCGAACAAATGATAAAATGGACTCAACATCTTGTCGCTCAG
GATGGTTTGTGGTTAGCCATGAAAGGGCGGTTTCCGGATACTGAATTGGTTCCGATTCATCAAACATACCGAGTTGAGAG
GTATGCTGTCCCGGGTATCGAGGGAGAGCGTTGCTGTGTGCTTATTAACAACACGAACAAGGAATAA

Upstream 100 bases:

>100_bases
TTAAACCAACTTCCTTGGCTCAAGCCGGGCGGATTTCTGGCGTAACACCTGCCGCGCTGTCTCTTTTACTTGTTCATCTG
AAAAAGAGTCGGCTTCCAGT

Downstream 100 bases:

>100_bases
TTATGGCGAAAGTCATAGCCATTGCCAACCAAAAAGGTGGAGTAGGTAAAACAACCACAGCCATCAATTTATCTGCATCT
CTTGCCGCATCCAAACAGCA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIDNTKIRSLLEKGLAEFKLDSIGDLLLDFLLLLNKWNKTYNLTAIRDIETMVSKHLFDSLAILPWIKGNHIIDVGTGPG
LPGIPLAIAKPDLQFVLLDSNGKKIRFLNEVKRQLNIKNIEPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTQHLVAQ
DGLWLAMKGRFPDTELVPIHQTYRVERYAVPGIEGERCCVLINNTNKE

Sequences:

>Translated_208_residues
MIDNTKIRSLLEKGLAEFKLDSIGDLLLDFLLLLNKWNKTYNLTAIRDIETMVSKHLFDSLAILPWIKGNHIIDVGTGPG
LPGIPLAIAKPDLQFVLLDSNGKKIRFLNEVKRQLNIKNIEPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTQHLVAQ
DGLWLAMKGRFPDTELVPIHQTYRVERYAVPGIEGERCCVLINNTNKE
>Mature_208_residues
MIDNTKIRSLLEKGLAEFKLDSIGDLLLDFLLLLNKWNKTYNLTAIRDIETMVSKHLFDSLAILPWIKGNHIIDVGTGPG
LPGIPLAIAKPDLQFVLLDSNGKKIRFLNEVKRQLNIKNIEPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTQHLVAQ
DGLWLAMKGRFPDTELVPIHQTYRVERYAVPGIEGERCCVLINNTNKE

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=197, Percent_Identity=45.1776649746193, Blast_Score=188, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 23743; Mature: 23743

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNTKIRSLLEKGLAEFKLDSIGDLLLDFLLLLNKWNKTYNLTAIRDIETMVSKHLFDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
LAILPWIKGNHIIDVGTGPGLPGIPLAIAKPDLQFVLLDSNGKKIRFLNEVKRQLNIKNI
HHHCCEECCCEEEECCCCCCCCCCCEEEECCCEEEEEECCCCCEEHHHHHHHHHCCCCCC
EPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTQHLVAQDGLWLAMKGRFPDTELVPIH
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECH
QTYRVERYAVPGIEGERCCVLINNTNKE
HHHEEEEEECCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MIDNTKIRSLLEKGLAEFKLDSIGDLLLDFLLLLNKWNKTYNLTAIRDIETMVSKHLFDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
LAILPWIKGNHIIDVGTGPGLPGIPLAIAKPDLQFVLLDSNGKKIRFLNEVKRQLNIKNI
HHHCCEECCCEEEECCCCCCCCCCCEEEECCCEEEEEECCCCCEEHHHHHHHHHCCCCCC
EPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTQHLVAQDGLWLAMKGRFPDTELVPIH
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECH
QTYRVERYAVPGIEGERCCVLINNTNKE
HHHEEEEEECCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA