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The map label for this gene is priA [H]

Identifier: 52843061

GI number: 52843061

Start: 3242470

End: 3244647

Strand: Direct

Name: priA [H]

Synonym: lpg2866

Alternate gene names: 52843061

Gene position: 3242470-3244647 (Clockwise)

Preceding gene: 52843060

Following gene: 52843062

Centisome position: 95.43

GC content: 41.32

Gene sequence:

>2178_bases
ATGAACGATATTTATCAGGTTTGTATCCCCCATACCAATCGAGATTGTTTTGATTACGAAGCCCTGGATTTAACTCCATG
CATTGGCGGAAGAGTATGGGTTCCTTTTCGCAATCAAGCCCGAGTGGGCATTGTAATCAATAAAGTTTGTGACCAGCAAA
CGGGCCATTCGTTAAAGAAAATCACTTCAATAATCGATGAGAAACCTCTCGTTCCTGAAGATCTCTTAGAGCTTTGTCTA
TGGGTAGGAAGCTATTATCAGTCACCATTATCTGAAGTGATTCCTTTGGTAATACCAAAAAAATATCGCCTGGGTTTACC
CTGCTCACTACCTACAGAAGATTTTTATCAATTGAATATACCTGTTGAAAAGGCGAAGACACTCATCTCGAAAAAAGCAA
AAAAACAACATGAGTTGATTGATTTTTTAAGCAGGCACCAGAAAGGGATTTCCAAACAGACCCTAAAACAACATGGCTAC
AACGGAACTCAAATTGCGGCACTACTTGCTTGTCAAGCAATTAACCTGTCACAGCAAGTTACCTTGCCAACCAAAATTCC
TGAAAAACTCAGTCCACCGCTAACACTCAATCCTGAACAAGCCGTTGCCGTAGATTCCATTTTCGAACACATCCATCATT
ACCAATGCTTTTTACTGCAAGGTGTTACCGGGAGTGGTAAGACAGAGGTTTATTTGCATGTCATTGCCAAAATATTGGAG
CAAGGCAAACAAGTATTGGTGCTGGTACCAGAAATCGGGTTAACCCCTCAGCTTTTATCTCGTTTTACAGCCCGATTTAA
CCACCCCATAACCGTATTACATTCCAATTTGAATGAATCAGAAAGGCAAATTGCCTGGCAGCTGGCAAAAGAGAACAAAG
TTAAAATGGTGATTGGAACTCGTTCTGCCGTTTTTACCCCTCTACCCAATCTTGGTTTAATTATCATCGATGAAGAGCAT
GACAGTTCGCTCAAACAAATGGAAGGGGTGCGTTATTCTGCGCGAGATACTGCTTTAATGCGTGCTCATCTTGCCAATAT
TCCTATCATTCTTGGTACAGCCACTCCCAGTCTGGAAACCATCCACAACTACAAACAAAAAAAATATAATTTATTACGCT
TAACGCATAAAGCTGCCTCCACTACACCGTTACACTATCAACTGATTGATTTACGATCGCAAATGGTACAGCATGGCTTG
GCTCTCCCAACCCTTAAAATCATAGAAAATCATTTGCTGCAACAAAACCAGGTTCTTGTTTTCATTAACCGACGCGGCTT
TGCCCCAGTGTTACTTTGCCATCAATGTGGATGGATGGTTGATTGCAAAGCATGCGATAGCCATTTAACCTTGCATAAAC
AGGCAGGACAAATGATTTGTCATCATTGTGGATTAACCCAAAGAATACCCTTGTTTTGCAATAACTGCCAAAGTAAGGAA
TTAATCCCCGTAGGATCTGGCACCCAGAGAATTTATGAGTTTTTAAGCCATCAGTTTCCAGATACGAATGTTTTACGAAT
TGACCGAGATGAGGTTCGTAAAAAAAATTCTCTAAACAGCCATTTGGACAAAATTAATAAAGGGGAAGCTCAGCTGATCA
TAGGCACTCAAATGCTAGCCAAGGGACACCATTTCCCCCGTCTATCCCTGGTCGTTGTCGTAGATGCTGATGCAGGCTTT
TACAACCAGGATTTCCGGGCTATTGAACATTTGGGGCAATTATTAACTCAAGTATCTGGACGAGCAGGGCGAGCAGAACA
TCCCGGGCAAGTATTGATTCAAACTTATTTACCTGATCATCCCCTGCTTAATCTACTCATTCAGCATGGGTATGATGAAT
TTGCGAACGCTTTATTAATTTCAAGACAAGAGGCTGAAATGCCACCTTTTCAGTTTTTAGCGGTCATCAGAGCTCAGGGC
AAAACCGTCAACAAGGTGGAGCAATTCCTAAAAGCAACCAAAGATCAAATTCAAGCTCATCCTCTCACGGTACTGGGTCC
TGCACCAGCTCCTTTACCACGAAAAGCTAACCAGCATAGGATGCAACTATTAATTAAATCCCCTTCAAGAAAAGTTCTAA
AAAGTTCATTGACGCAATTAAGAGAGTGGTTAACAATGAATAAATTAAGTAATGGCATCCGCTGGAATGTGGATGTTGAT
CCTATGGACTTATCATGA

Upstream 100 bases:

>100_bases
AGAAAAAGAGGAGTTAGATAATCATCGTATCGAAAAAGTAGTAGTCAAAGTGTTTTCCGCGCCCGGCCAATCAGCGAGTC
ATGAATGGCTTAGAAGTTAA

Downstream 100 bases:

>100_bases
CTGAAATAAACAAAATAATACATAAAAAAACTTTTGATATCGCCTGGGGCGATATGGACGCATTAGGACATGTTAACAAT
GCTCGCTATTTTGACTATTT

Product: primosomal protein N' (replication factor Y)

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 725; Mature: 725

Protein sequence:

>725_residues
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Sequences:

>Translated_725_residues
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS
>Mature_725_residues
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=737, Percent_Identity=41.9267299864315, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1787357, Length=179, Percent_Identity=30.7262569832402, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81789; Mature: 81789

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKK
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHHHCCCCCHHHHH
ITSIIDEKPLVPEDLLELCLWVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNI
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEECC
PVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGYNGTQIAALLACQAINLSQQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
TLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
ECCCCCHHHCCCCCEECCHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHH
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGT
CCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEC
RSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLET
CCEEECCCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHH
IHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGLALPTLKIIENHLLQQNQVLV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEE
FINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
EEECCCCCHHHHHHHCCCEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLA
EEECCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
KGHHFPRLSLVVVVDADAGFYNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDH
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
PLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQGKTVNKVEQFLKATKDQIQAH
HHHHHHHHCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
PLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
CEEEECCCCCCCCCCCCHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
PMDLS
CCCCC
>Mature Secondary Structure
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKK
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHHHCCCCCHHHHH
ITSIIDEKPLVPEDLLELCLWVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNI
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEECC
PVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGYNGTQIAALLACQAINLSQQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
TLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
ECCCCCHHHCCCCCEECCHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHH
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGT
CCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEC
RSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLET
CCEEECCCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHH
IHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGLALPTLKIIENHLLQQNQVLV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEE
FINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
EEECCCCCHHHHHHHCCCEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLA
EEECCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
KGHHFPRLSLVVVVDADAGFYNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDH
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
PLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQGKTVNKVEQFLKATKDQIQAH
HHHHHHHHCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
PLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
CEEEECCCCCCCCCCCCHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
PMDLS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]