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The map label for this gene is dus1 [H]

Identifier: 52843056

GI number: 52843056

Start: 3236727

End: 3237716

Strand: Direct

Name: dus1 [H]

Synonym: lpg2861

Alternate gene names: 52843056

Gene position: 3236727-3237716 (Clockwise)

Preceding gene: 52843045

Following gene: 52843059

Centisome position: 95.26

GC content: 41.62

Gene sequence:

>990_bases
TTGATGCACACTTTTATTAACAGTCCTCTGCAAATCGGCTCTTTAAAACTCCCTCATCGACTGATTCAAGGACCTTTGGC
AGGATACAGTTGCGCCCCTTTCAGAGATCTATTCAATATTTATACACCGCCTGCTTATTGCGTTTCTGAAATGTGTTCTG
CCATCGACATTCTTCATAAACATTCCTCTCAATCCCGGTATATTTACAGAGCTCCCACAGAAAAATTCCTGGCTTACCAA
ATCTCCGGAAATGAGCCACATGTCCTTGCAGAAGCAGCAGCTAAACTGCAATCACTGGGTGCGGATCTAATTGATATTAA
TTGCGGCTGCCCTAAAAGCAAAATTCGTAAAAAAGGGGCTGGTAGCGCTTTATTGGAAGATCCACAAAAATTGATTGCCA
TTATCTCTAGGGTACGCGCAGCGATCAGCATTCCGCTGACTATTAAAATACGCATTCAAAACAATGAAAAGGATTTAACC
TTAGCCAAACAAATAGAGGACGCTGGTGCTCATGCCCTGATAGTGCATGGCAGGCGATGGACAGAAGATTATGATATCCC
ATGCAATTGGCAGCAAATTGCAAATATCAAGAATGCTGTCAATATACCAGTCATTGTAAATGGTGATATTAACAATATAT
TCAGTTTAAGAAAGGCCCTGGAAATCAGCGCGTGCGATGGTTTTATGATTTCTCGAGCAGGTACGGGCAAACCCTGGCTA
TATCGGGATTTATTGAATCTGGAAACAAATGATGTCGCTTTTGATGAGAAATTAAAACTGTTTATGCTCCACCTCGAAGG
ATTGTCATGCCTTGAAAACGAGTATAAAGCGGTACTGCAAAGCAAGTCCCTGGTTCGCTATTACTTCAAAAAGCAACTGA
ACGAACAACAACTGCAACAGTTCTATCAATTAGGTTCATTAATGGAAATCAAGGATTTTTTAGCTGAGGCAACTCACCGA
GTTTCCCCCATATTAGTTGCCGCCTCATGA

Upstream 100 bases:

>100_bases
GTGACAAATACCTCCTGTCCATATGGAAGCCTCTCCCTTTTTTCTGGTTCTTCATGATGAATCATGTTAAAATAAAAAGC
GATTATAGCCTTTAATTCCA

Downstream 100 bases:

>100_bases
TATAAGGTCAGGATTTGTATATTATTTCTATAATTTGATAACCATTACATTCCGCTGTTTAGTTCAGCTCACGCTGAAGA
TTAAAGGGTTATGAGGAGAA

Product: nitrogen regulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MMHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHKHSSQSRYIYRAPTEKFLAYQ
ISGNEPHVLAEAAAKLQSLGADLIDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLT
LAKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWL
YRDLLNLETNDVAFDEKLKLFMLHLEGLSCLENEYKAVLQSKSLVRYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHR
VSPILVAAS

Sequences:

>Translated_329_residues
MMHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHKHSSQSRYIYRAPTEKFLAYQ
ISGNEPHVLAEAAAKLQSLGADLIDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLT
LAKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWL
YRDLLNLETNDVAFDEKLKLFMLHLEGLSCLENEYKAVLQSKSLVRYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHR
VSPILVAAS
>Mature_329_residues
MMHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHKHSSQSRYIYRAPTEKFLAYQ
ISGNEPHVLAEAAAKLQSLGADLIDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLT
LAKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWL
YRDLLNLETNDVAFDEKLKLFMLHLEGLSCLENEYKAVLQSKSLVRYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHR
VSPILVAAS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=253, Percent_Identity=27.6679841897233, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI239788462, Length=258, Percent_Identity=27.1317829457364, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI8923374, Length=204, Percent_Identity=32.3529411764706, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI40807366, Length=181, Percent_Identity=32.0441988950276, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1789660, Length=304, Percent_Identity=30.9210526315789, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=31.1965811965812, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693211, Length=205, Percent_Identity=24.390243902439, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=263, Percent_Identity=29.6577946768061, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=217, Percent_Identity=31.7972350230415, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=181, Percent_Identity=35.3591160220994, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=159, Percent_Identity=27.6729559748428, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=172, Percent_Identity=31.3953488372093, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=278, Percent_Identity=23.021582733813, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=281, Percent_Identity=24.9110320284698, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24580595, Length=176, Percent_Identity=31.8181818181818, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920448, Length=176, Percent_Identity=31.8181818181818, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585320, Length=241, Percent_Identity=29.4605809128631, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19921524, Length=231, Percent_Identity=25.974025974026, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI45549423, Length=157, Percent_Identity=33.1210191082803, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36912; Mature: 36912

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHK
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
HSSQSRYIYRAPTEKFLAYQISGNEPHVLAEAAAKLQSLGADLIDINCGCPKSKIRKKGA
CCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCC
GSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTLAKQIEDAGAHALIVHGRRW
CCHHHHCHHHHHHHHHHHHHHEECCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCC
TEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWL
CCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
YRDLLNLETNDVAFDEKLKLFMLHLEGLSCLENEYKAVLQSKSLVRYYFKKQLNEQQLQQ
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FYQLGSLMEIKDFLAEATHRVSPILVAAS
HHHHCCHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MMHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHK
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
HSSQSRYIYRAPTEKFLAYQISGNEPHVLAEAAAKLQSLGADLIDINCGCPKSKIRKKGA
CCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCC
GSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTLAKQIEDAGAHALIVHGRRW
CCHHHHCHHHHHHHHHHHHHHEECCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCC
TEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWL
CCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
YRDLLNLETNDVAFDEKLKLFMLHLEGLSCLENEYKAVLQSKSLVRYYFKKQLNEQQLQQ
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FYQLGSLMEIKDFLAEATHRVSPILVAAS
HHHHCCHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]