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The map label for this gene is ispB [H]

Identifier: 52842862

GI number: 52842862

Start: 3003002

End: 3003970

Strand: Direct

Name: ispB [H]

Synonym: lpg2656

Alternate gene names: 52842862

Gene position: 3003002-3003970 (Clockwise)

Preceding gene: 52842861

Following gene: 52842867

Centisome position: 88.38

GC content: 40.25

Gene sequence:

>969_bases
ATGACTATTGACCGTATACGAGCGCTGGTTAGTGAAGATTTTAACGCGGTTAATAATTTAATTATTGATAAAATTCAATC
TCAAGTTGGTTTAATTCAGGACTTGTCACAACATATAGTCGAAAGTGGCGGCAAACGTTTACGGCCTTTGGTTGTCCTGT
TGGCAAGCAATGCTTGTGGATACAAAGGAAAAGACCATATTTCTCTTGCCGCTATGGTTGAGTTTTTCCATACTGCCACT
TTGTTGCATGATGATGTGATTGATGAATCCACTTTAAGAAGAGGTCGACAAACTGCAAATAGCATTTGGGGCAGTAAAGC
CAGTGTTTTAGTTGGTGATTATCTTTTTACCCAATCCGTAAATCTAATGGTAGAGGTTGGCATTAAAGAAATTCTCAATC
TACTGGCCAATACTTCCCACCAAATTACCTGTGGCGAAGTCAAACAACTTGCCAATCGCCACAATCCCGCTTTAACCCTT
GATGACTATTTCGACGTCATCCGGGCCAAAACAGCTCTTTTATTTGCTGCGGCTGCCTGCATAGGTCCTATACTCACGAA
TGCGCCTATTGAGCTTCGAAATAGTTTATATAATTACGGTTTACACTTGGGAAATGCCTTTCAATTGATTGATGATGCCT
TGGATTATTGCTCGGATGCTGCCACTTTAGGTAAAAACATTGGCGACGATTTGGCTGATGGAAAAGCGACTTTACCACTA
ATACATGCGTTACAAAATGGTAATGCGACTCAACAACAGCAAATAAGAGAAAGTTTAATGAAAGGCAGTCTAAGCCATCT
GCCTGATATTTTAATTGCTATTGAGCAAACCGAAGCCATACCGTTTACGAAACGAATTGCTGCCCAAGAAGTAGACTCAG
CACTATCTGCCCTGGAAATACTGCCTGACTCAGTTTATAAAGAAGCTTTAGTCGATTTGGCCAAATTTGCCTTGGAAAGA
AATTATTAA

Upstream 100 bases:

>100_bases
CTTCATTTTATCAAGCACAGGATGCAGCAATCTGACATTCACTTGACAATTAACTTTTACAACTTTAGCATTCATTGATT
AAATATCAGAGGGTAAATGA

Downstream 100 bases:

>100_bases
CCTGATAATTGCATCGATTCTAATAATTACACAACCGATTGATAATAATCTGGCGGAGTGCGGCATTTAAAGCAGGCAGT
TTTGCAATTGGCTCCTCCCT

Product: octaprenyl diphosphate synthase IspB

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGGKRLRPLVVLLASNACGYKGKDHISLAAMVEFFHTAT
LLHDDVIDESTLRRGRQTANSIWGSKASVLVGDYLFTQSVNLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTL
DDYFDVIRAKTALLFAAAACIGPILTNAPIELRNSLYNYGLHLGNAFQLIDDALDYCSDAATLGKNIGDDLADGKATLPL
IHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQTEAIPFTKRIAAQEVDSALSALEILPDSVYKEALVDLAKFALER
NY

Sequences:

>Translated_322_residues
MTIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGGKRLRPLVVLLASNACGYKGKDHISLAAMVEFFHTAT
LLHDDVIDESTLRRGRQTANSIWGSKASVLVGDYLFTQSVNLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTL
DDYFDVIRAKTALLFAAAACIGPILTNAPIELRNSLYNYGLHLGNAFQLIDDALDYCSDAATLGKNIGDDLADGKATLPL
IHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQTEAIPFTKRIAAQEVDSALSALEILPDSVYKEALVDLAKFALER
NY
>Mature_321_residues
TIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGGKRLRPLVVLLASNACGYKGKDHISLAAMVEFFHTATL
LHDDVIDESTLRRGRQTANSIWGSKASVLVGDYLFTQSVNLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTLD
DYFDVIRAKTALLFAAAACIGPILTNAPIELRNSLYNYGLHLGNAFQLIDDALDYCSDAATLGKNIGDDLADGKATLPLI
HALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQTEAIPFTKRIAAQEVDSALSALEILPDSVYKEALVDLAKFALERN
Y

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=297, Percent_Identity=29.2929292929293, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4758430, Length=186, Percent_Identity=27.4193548387097, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI83700220, Length=186, Percent_Identity=27.4193548387097, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=44.7204968944099, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1786623, Length=236, Percent_Identity=30.9322033898305, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17505681, Length=348, Percent_Identity=28.1609195402299, Blast_Score=141, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6319475, Length=297, Percent_Identity=28.956228956229, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6325188, Length=285, Percent_Identity=25.6140350877193, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24651612, Length=294, Percent_Identity=26.1904761904762, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35173; Mature: 35041

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGGKRLRPLVVLLASNACG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
YKGKDHISLAAMVEFFHTATLLHDDVIDESTLRRGRQTANSIWGSKASVLVGDYLFTQSV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTLDDYFDVIRAKTALLFAAAAC
HHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
IGPILTNAPIELRNSLYNYGLHLGNAFQLIDDALDYCSDAATLGKNIGDDLADGKATLPL
HHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHH
IHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQTEAIPFTKRIAAQEVDSALSALEI
HHHHHCCCCHHHHHHHHHHHHCCHHHCCHHHEEEHHCCCCCHHHHHHHHHHHHHHHHHHH
LPDSVYKEALVDLAKFALERNY
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGGKRLRPLVVLLASNACG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
YKGKDHISLAAMVEFFHTATLLHDDVIDESTLRRGRQTANSIWGSKASVLVGDYLFTQSV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTLDDYFDVIRAKTALLFAAAAC
HHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
IGPILTNAPIELRNSLYNYGLHLGNAFQLIDDALDYCSDAATLGKNIGDDLADGKATLPL
HHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHH
IHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQTEAIPFTKRIAAQEVDSALSALEI
HHHHHCCCCHHHHHHHHHHHHCCHHHCCHHHEEEHHCCCCCHHHHHHHHHHHHHHHHHHH
LPDSVYKEALVDLAKFALERNY
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]