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The map label for this gene is mdh

Identifier: 52842562

GI number: 52842562

Start: 2656503

End: 2657495

Strand: Reverse

Name: mdh

Synonym: lpg2352

Alternate gene names: 52842562

Gene position: 2657495-2656503 (Counterclockwise)

Preceding gene: 52842563

Following gene: 52842560

Centisome position: 78.21

GC content: 43.4

Gene sequence:

>993_bases
ATGACAAATAATCGAGTAAGAGTTGCTGTCACAGGTGCAGCAGGGCAAATAGGTTATGCCTTGGTGTTTCGTATTGCTTC
CGGTCAAATGTTTGGGCCAAATACTGAAGTGGAATTGAATTTACTTGAACTTGAGCCGGCACTTCCCTCACTGGAAGGAG
TCGCTATGGAGCTTGATGATTGCGCATTTCCCTTATTAAAACGCATTGTTTGTACTGCAGATTTGAATAAGGCAATGGAT
GGTGTTAACTGGGCATTATTAGTCGGTTCAGTTCCGCGAAAGCAAGGCATGGAGCGTTCTGATTTATTACAAATCAATGG
TGGTATATTCACCAAACAAGGTCAGGCGATTAATGATTATGCCAGTGATGATGTACGGGTATTTGTTGTGGGAAACCCTT
GCAATACCAATTGCCTAATTGCTATGAATCACGCAAAAGACGTGCCTTCTGATCGTTTTTATGCAATGACCACATTGGAT
GAGTTAAGAGCTCGTACTCAATTGGCTAAAAAAGCAGGCGTTGACATTACTGCAGTGACTCAAATGACGATTTGGGGTAA
TCATTCCGCCACCCAATATCCCGATTTTTATAACGCAAAAATCAATGGAACTTCTGCTGCGCAAGTCATTAATGATGAAA
CCTGGTTAAAAGAGACTTTTGTATCAACCGTCCAGCAACGAGGAGCCGCAGTGATCAAGGCACGGGGTTCATCTTCTGCT
GCTTCTGCCGCCAATGCGATAATTACAGGAGTTAATCATTTGGTTACGGATACTCCTGCTGGCGAATCGTTTTCAATGTG
CCGTCGCTCAAAGGGAGAGTACGGAGTCGATGAAGGTTTGATTTTCTCTTTCCCATGCCGAAGAGAACATGGCGAATTAA
AAGTGGTCGAGAACTTGGAGTTCAACGATTTTGGCCGCGAGAGATTCAATACCACTTTAAACGAATTACGAAGTGAGCGT
GACACGGTCAAGTCTTTAGGCTTATTGGATTAA

Upstream 100 bases:

>100_bases
CACGCTGCTTATGAGTTATTGGAAAAACCGGATTTTACCGAGGCGCGCAGTATTGCTGCGCTATTTTTAGTTAGAGAGTG
GTTGAATAAGAGGAAATCAT

Downstream 100 bases:

>100_bases
CCAGACCGGGTTAATTCTTGATAGATTTGATTCTGTTGTTTTTGCTGATTACCCTGCTTTGTGAATTTTTCAGGGCAGGG
TGCTGTCGCACCTTGGTTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMD
GVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
ELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKETFVSTVQQRGAAVIKARGSSSA
ASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENLEFNDFGRERFNTTLNELRSER
DTVKSLGLLD

Sequences:

>Translated_330_residues
MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMD
GVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
ELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKETFVSTVQQRGAAVIKARGSSSA
ASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENLEFNDFGRERFNTTLNELRSER
DTVKSLGLLD
>Mature_329_residues
TNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMDG
VNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLDE
LRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKETFVSTVQQRGAAVIKARGSSSAA
SAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENLEFNDFGRERFNTTLNELRSERD
TVKSLGLLD

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=322, Percent_Identity=47.5155279503106, Blast_Score=294, Evalue=9e-80,
Organism=Homo sapiens, GI89886456, Length=330, Percent_Identity=23.3333333333333, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1789632, Length=277, Percent_Identity=27.4368231046931, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17561064, Length=321, Percent_Identity=46.7289719626168, Blast_Score=270, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI32566798, Length=235, Percent_Identity=47.6595744680851, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=44.1176470588235, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24583394, Length=329, Percent_Identity=47.4164133738602, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24647881, Length=334, Percent_Identity=26.9461077844311, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_LEGPA (Q5X2T6)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_124612.1
- ProteinModelPortal:   Q5X2T6
- SMR:   Q5X2T6
- STRING:   Q5X2T6
- GeneID:   3119651
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp2301
- LegioList:   lpp2301
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   LPNE297246:LPP2301-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 36007; Mature: 35876

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 188-188 BINDING 93-93 BINDING 99-99 BINDING 106-106 BINDING 113-113 BINDING 132-132 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDD
CCCCEEEEEEECCCCCCCEEEEEEEECCCEECCCCCEEEEEEEECCCCCCCCCEEEEHHH
CAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDY
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHEEEECCCEEECCCCCCCCC
ASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLDELRARTQLAKKAGVDITAVT
CCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEE
QMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKETFVSTVQQRGAAVIKARGSSSA
EEEEECCCCCCCCCCCCCCEECCCHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCH
ASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENLE
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCC
FNDFGRERFNTTLNELRSERDTVKSLGLLD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDD
CCCEEEEEEECCCCCCCEEEEEEEECCCEECCCCCEEEEEEEECCCCCCCCCEEEEHHH
CAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDY
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHEEEECCCEEECCCCCCCCC
ASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLDELRARTQLAKKAGVDITAVT
CCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEE
QMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKETFVSTVQQRGAAVIKARGSSSA
EEEEECCCCCCCCCCCCCCEECCCHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCH
ASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENLE
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCC
FNDFGRERFNTTLNELRSERDTVKSLGLLD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA