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The map label for this gene is fadE10 [H]

Identifier: 52842439

GI number: 52842439

Start: 2515574

End: 2517265

Strand: Reverse

Name: fadE10 [H]

Synonym: lpg2226

Alternate gene names: 52842439

Gene position: 2517265-2515574 (Counterclockwise)

Preceding gene: 52842440

Following gene: 52842438

Centisome position: 74.09

GC content: 39.78

Gene sequence:

>1692_bases
ATGACCTCTTATGAAAATTTGATACAACTGTCTGAATTTTTTGAGTCATGCCTGGGTAATCCTCTGGAAAACAATTCCCC
TGTTAATTTTCAACAAATTTTGCTCGCTGATGAGCAGGAAGTATTAGCTTCGTCAGCAATAGAATTTATTAAACAATGGG
GGTATTTGGACTATACCATACCTCATTATCTAGGGGGTAAGCTGAGTTCCCTGGATGAGCTATATACCTTAACCAGGTTG
TTGGCCAGACGTGACATCACGATGGCTATTATGTTTGGATTGGCTTTCTTTGCTGCATTGCCAATATGGATAGCTGGAAA
CTCAAAGCAAAAGAAACATTTGAGTGAGAGCCTTCGCCAGGGAAAGATAGGTGCTTTGGCTTTGACCGAAGAAGAGCATG
GAACTGATTTGACTTCCAATAAGATGAATGCCAAGCCAATTGAAGATGGCTGGGAAATTTCGGGGCGAAAATGGTGTGTT
AATTTTGCAACCTTGAGCGAGTACGCTGTGGTGTTGTGTCGTACTAATGAAAAGGGCGGGTTATTAGGTTTTTCTTTGTT
TTATATCGATAAAAAATTAGCCGAAAAGGGTATTTCTCCTACGCCCAAGCTGCCGACTCATGGAGTCAGAGGGTTAGATA
TCAGTGGTTTTTCTTTTGATAAAGTGAAAGTATCTAAAGACGCATTAATAGGGAAGGAACAACGTGGTTTGGAAATAACC
TATAAGGTATTCCAGATTTCGCGAACCTTATGCGCCTGTTTGGCTATTGGGGGCGCTGATACTGCTTTGAGGCTGGCATT
ATCATATAGCTTGGAAAGACAACTGTATGGCAAATCAGTGTTTGAAATACCCGCAGTAAAACAGCGGCTTGGCGAATTGT
TTACTCTTTTATTAATCGCGGATTGCACTGCTCAGGTTATGGTTCGCGCCTGTACGGTAATACCGAAAAAAATGAGTCTT
TGGTCAGCAATTATTAAATTTCTTATTCCGCATATTGGAGAAGATATAGCAGAACAGTGTGCCATTATTCTAGGTGCCAG
AGCTTATCTGAGAACCACGCAATGGGCGATTTTTCAGAAAATAAGACGCGACATTCAAGTGGTTGGCTTGTTTGATGGAA
GTACTCAAGTTAATTTATCACTTATTGCTGGTAATTTGTTGCCTCAGGCAGGCATGCGGGGTAAACATCAGGCAGAGCAT
TCCGAAAAAATTGAGAAAATTTTTAATGTTCGTTTGTCTTGTCCTCCTTTTAAGGAGGATAGACTCGGTTTGTTTACTCA
TGAGGAAGATGATGTATTGGCTGGTATCATGCGTTTAAACTCACCTGCAATCAATCCACTGATTACTACAATACGTCATG
AAATTGAAAAATTGGATCAGGAAGTTATCCATTTACATGATCAGAAATTATTTGACCCACGCAGCTTAACTGTTTTTCGT
TTGGCAGAAAAATATTGCTGGATTTTTGCAGCCAGCTGTTGTTTGCAGTTCTGGCACTATAATCAGGAGATACTTTCTGA
TGATTTGCAAAATACAGATTGGCTGGATTTAGCTATGCAGTTAATTCTTAAGCGATTGAATTCATGTTCGATGATTGATA
GCTTGTTACAAGAAGCTATGAGTGAGCGTTTGTATTCTTTTTATCAGGAAAAACAGCTGTTTTCTATTATGCCAATACAT
ATTGCGGGGTAA

Upstream 100 bases:

>100_bases
CAGCGCAAATTCGCAGTCGTCTTGCCTCCCATTTGGAGTTTTTATATAGAAGCATGGAAACCATGCCCCCGGCAAAACAC
TCTATTGTATAAGGAAAATA

Downstream 100 bases:

>100_bases
TGAAGGATGAATTGGAAAAGGTTTTTAATTGCTGCCATGACAAAGAAAAAATACCAATTTTTTCTGAATGATACCAAACA
TCCTAAAGAGGCCAGAGAAC

Product: isovaleryl CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTSYENLIQLSEFFESCLGNPLENNSPVNFQQILLADEQEVLASSAIEFIKQWGYLDYTIPHYLGGKLSSLDELYTLTRL
LARRDITMAIMFGLAFFAALPIWIAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPIEDGWEISGRKWCV
NFATLSEYAVVLCRTNEKGGLLGFSLFYIDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEIT
YKVFQISRTLCACLAIGGADTALRLALSYSLERQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPKKMSL
WSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHQAEH
SEKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGIMRLNSPAINPLITTIRHEIEKLDQEVIHLHDQKLFDPRSLTVFR
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSCSMIDSLLQEAMSERLYSFYQEKQLFSIMPIH
IAG

Sequences:

>Translated_563_residues
MTSYENLIQLSEFFESCLGNPLENNSPVNFQQILLADEQEVLASSAIEFIKQWGYLDYTIPHYLGGKLSSLDELYTLTRL
LARRDITMAIMFGLAFFAALPIWIAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPIEDGWEISGRKWCV
NFATLSEYAVVLCRTNEKGGLLGFSLFYIDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEIT
YKVFQISRTLCACLAIGGADTALRLALSYSLERQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPKKMSL
WSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHQAEH
SEKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGIMRLNSPAINPLITTIRHEIEKLDQEVIHLHDQKLFDPRSLTVFR
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSCSMIDSLLQEAMSERLYSFYQEKQLFSIMPIH
IAG
>Mature_562_residues
TSYENLIQLSEFFESCLGNPLENNSPVNFQQILLADEQEVLASSAIEFIKQWGYLDYTIPHYLGGKLSSLDELYTLTRLL
ARRDITMAIMFGLAFFAALPIWIAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPIEDGWEISGRKWCVN
FATLSEYAVVLCRTNEKGGLLGFSLFYIDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEITY
KVFQISRTLCACLAIGGADTALRLALSYSLERQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPKKMSLW
SAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHQAEHS
EKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGIMRLNSPAINPLITTIRHEIEKLDQEVIHLHDQKLFDPRSLTVFRL
AEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSCSMIDSLLQEAMSERLYSFYQEKQLFSIMPIHI
AG

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501857, Length=285, Percent_Identity=27.3684210526316, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI4557233, Length=370, Percent_Identity=23.7837837837838, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI187960098, Length=290, Percent_Identity=25.8620689655172, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI4557231, Length=290, Percent_Identity=25.8620689655172, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI76496475, Length=380, Percent_Identity=18.6842105263158, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4557235, Length=380, Percent_Identity=18.6842105263158, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI226958412, Length=298, Percent_Identity=23.8255033557047, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI226958414, Length=276, Percent_Identity=23.9130434782609, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI4501859, Length=356, Percent_Identity=21.9101123595506, Blast_Score=66, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17508101, Length=352, Percent_Identity=21.0227272727273, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17570075, Length=361, Percent_Identity=22.7146814404432, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71985184, Length=405, Percent_Identity=20.4938271604938, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17534899, Length=357, Percent_Identity=24.0896358543417, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538396, Length=278, Percent_Identity=21.5827338129496, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17569725, Length=351, Percent_Identity=22.5071225071225, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982178, Length=223, Percent_Identity=24.6636771300448, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71990804, Length=260, Percent_Identity=20.7692307692308, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17506239, Length=334, Percent_Identity=20.6586826347305, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356377, Length=363, Percent_Identity=25.068870523416, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24660351, Length=354, Percent_Identity=25.4237288135593, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24646207, Length=360, Percent_Identity=22.7777777777778, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24666513, Length=282, Percent_Identity=21.9858156028369, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355753, Length=284, Percent_Identity=22.1830985915493, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920834, Length=272, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 63585; Mature: 63453

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYENLIQLSEFFESCLGNPLENNSPVNFQQILLADEQEVLASSAIEFIKQWGYLDYTI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEC
PHYLGGKLSSLDELYTLTRLLARRDITMAIMFGLAFFAALPIWIAGNSKQKKHLSESLRQ
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHC
GKIGALALTEEEHGTDLTSNKMNAKPIEDGWEISGRKWCVNFATLSEYAVVLCRTNEKGG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHCCEEEEEEEECCCCC
LLGFSLFYIDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEIT
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHCCCCCCCCCHH
YKVFQISRTLCACLAIGGADTALRLALSYSLERQLYGKSVFEIPAVKQRLGELFTLLLIA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DCTAQVMVRACTVIPKKMSLWSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHQAEHSEKIEKIFNVRLSCPPFKED
HHHCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
RLGLFTHEEDDVLAGIMRLNSPAINPLITTIRHEIEKLDQEVIHLHDQKLFDPRSLTVFR
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSCSMIDSLLQEAM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SERLYSFYQEKQLFSIMPIHIAG
HHHHHHHHHHHHHHEECCEEECC
>Mature Secondary Structure 
TSYENLIQLSEFFESCLGNPLENNSPVNFQQILLADEQEVLASSAIEFIKQWGYLDYTI
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEC
PHYLGGKLSSLDELYTLTRLLARRDITMAIMFGLAFFAALPIWIAGNSKQKKHLSESLRQ
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHC
GKIGALALTEEEHGTDLTSNKMNAKPIEDGWEISGRKWCVNFATLSEYAVVLCRTNEKGG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHCCEEEEEEEECCCCC
LLGFSLFYIDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEIT
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHCCCCCCCCCHH
YKVFQISRTLCACLAIGGADTALRLALSYSLERQLYGKSVFEIPAVKQRLGELFTLLLIA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DCTAQVMVRACTVIPKKMSLWSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHQAEHSEKIEKIFNVRLSCPPFKED
HHHCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
RLGLFTHEEDDVLAGIMRLNSPAINPLITTIRHEIEKLDQEVIHLHDQKLFDPRSLTVFR
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSCSMIDSLLQEAM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SERLYSFYQEKQLFSIMPIHIAG
HHHHHHHHHHHHHHEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]